Study Of In Vitro Toxicity Of Glucose Capped Gold Nanoparticles In Malignant And Normal Cell Lines

Green synthesis of gold nanoparticles (GNPs) with plant extracts has gained considerable interest in the field of biomedicine. Recently, the bioreduction nature of herbal extracts has helped to synthesize spherical GNPs of different potential from gold salt. In this study, a fast ecofriendly method was adopted for the synthesis of GNPs using fresh peel (aqueous) extracts of Benincasa hispida, which acted as reducing and stabilizing agents. The biosynthesized GNPs were characterized by UV–VIS and Fourier transform infrared spectroscopy, transmission electron microscopy (TEM), and dynamic light scattering. In addition, the in vitro antibacterial and anticancer activities of synthesized GNPs were investigated. The formation of gold nanoparticles was confirmed by the existence of a sharp absorption peak at 520 nm, corresponding to the surface plasmon resonance (SPR) band of the GNPs. TEM analysis revealed that the prepared GNPs were spherical in shape and had an average particle size of 22.18 ± 2 nm. Most importantly, the synthesized GNPs exhibited considerable antibacterial activity against different Gram-positive and Gram-negative bacteria. Furthermore, the biosynthesized GNPs exerted remarkable in vitro cytotoxicity against human cervical cancer cell line, while sparing normal human primary osteoblast cells. Such cytotoxic effect was attributed to the increased production of reactive oxygen species (ROS) that contributed to the damage of HeLa cells. Collectively, peel extracts of B. hispida can be efficiently used for the synthesis of GNPs, which can be adopted as a natural source of antimicrobial and anticancer agent.

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Synthesis of gold nanoparticles and assessment of in vitro toxicity against plant pathogens

Indian Phytopathology ◽

10.1007/s42360-021-00444-x ◽

2021 ◽

Author(s):

Rajni Kant Thakur ◽

Pramod Prasad

Keyword(s):

Gold Nanoparticles ◽

Plant Pathogens ◽

In Vitro Toxicity

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In vitro cell cytotoxicity profile and morphological response to polyoxometalate-stabilised gold nanoparticles

New Journal of Chemistry ◽

10.1039/c5nj02775f ◽

2016 ◽

Vol 40 (2) ◽

pp. 1039-1047 ◽

Cited By ~ 8

Author(s):

Isabel Maicas Gabas ◽

Grazyna Stepien ◽

María Moros ◽

Scott G. Mitchell ◽

Jesús M. de la Fuente

Keyword(s):

Gold Nanoparticles ◽

Cell Lines ◽

Melanoma Cell ◽

Cell Cytotoxicity ◽

Skin Melanoma ◽

Morphological Response ◽

Antiproliferative Action ◽

Melanoma Cell Lines

Polyoxometalate-stabilised gold nanoparticles internalise in vast quantities into kidney epithelial and skin melanoma cell lines causing antiproliferative action on tumoural cells.

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Ellagic Acid Cytotoxic and Metalloproteinases Inhibitory Effects Against Oral Squamous Cell Carcinoma: An in Vitro Study

10.21203/rs.3.rs-879044/v1 ◽

2021 ◽

Author(s):

Patricia Maria Wiziack Zago ◽

Luiza Rodrigues Hellmeister ◽

Lucas Novaes Teixeira ◽

Rui Barbosa de Brito Junior ◽

Elizabeth Ferreira Martinez

Keyword(s):

Oral Cancer ◽

Cell Lines ◽

Ellagic Acid ◽

Normal Cell ◽

In Vitro Study ◽

Significance Level ◽

Viability Analysis ◽

Anticancer Properties

Abstract ObjectivesThis study aimed to evaluate the in vitro antitumoral potential of different concentrations of EA against two OSCC cell lines with distinct tissue invasiveness profiles. Material and methodsNormal keratinocytes (NOK) and OSCC´s cells CAL-27 and SCC-9 were treated with concentrations of EA varying from 5 to 662 µM during 24, 48 or 72h. After each time of treatment, cells were submitted to viability analysis using MTT and the secretion of metalloproteinases (MMP-2 and MMP-9) and tissue metalloproteinases inhibitors (TIMP-1 and TIMP-2) were performed by Enzyme-Linked Immunoassay (ELISA). Data were submitted to ANOVA, followed by Bonferroni´s test, considering 5% as significance level. ResultsEA was cytotoxic to OSCC cells in all exposure times, rarely affecting normal cell viability, except for concentrations higher than 82 µM and after 72h treatment. For OSCC cells, EA decreased MMPs and increased TIMPs´s expression without effect on those enzymes for normal cell lines during all times of exposure. ConclusionEA is a promising therapeutic adjuvant to treat oral cancer, however, further in vivo studies are required to clinically validate its potential. Clinical RelevanceThe in vitro anticancer properties showed by Ellagic acid, a phenolic compound that could easily be accessed by oral cancer patients, provides data to base future clinical studies intended to develop a safe topical oral anticancer product.

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IN VITRO CYTOTOXICITY AND ANTIOXIDANT EVALUATION OF BIOGENIC SYNTHESIZED GOLD NANOPARTICLES FROM MARSILEA QUADRIFOLIA ON LUNG AND OVARIAN CANCER CELLS

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2018v10i5.27999 ◽

2018 ◽

Vol 10 (5) ◽

pp. 153 ◽

Cited By ~ 1

Author(s):

Balashanmugam P. ◽

Mosa Christas K. ◽

Kowsalya E.

Keyword(s):

Gold Nanoparticles ◽

Cell Lines ◽

Aqueous Extract ◽

A549 Cell ◽

In Vitro Cytotoxicity ◽

Spherical Particles ◽

X Ray ◽

Marsilea Quadrifolia ◽

Wide Range

Objective: The biogenic gold nanoparticles are considered to be extremely impressive for its wide range of applications in pharmaceutics and therapeutics. The present study was aimed at the biogenic synthesis of gold nanoparticles (AuNPs) from Marsilea quadrifolia aqueous extract and to investigate its antioxidant property and cytotoxic effect on human ovarian teratocarcinoma (PA-1) and lung adenocarcinoma (A549) cell lines.Methods: The biogenic AuNPs was synthesized using an aqueous extract of Marsilea quadrifolia. The synthesized biogenic AuNPs were characterized by ultraviolet (UV) visible spectroscopy, transmission electron microscopy (TEM), energy dispersive X-ray analysis (EDX) and X-ray diffraction (XRD). The biogenic AuNPs was assessed for its stability over a period of time and antioxidant activity. The cytotoxicity of biogenic AuNPs against PA-1 and A549 cell lines was studied using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.Results: The synthesized biogenic AuNPs showed peculiar ruby red color and a surface plasmon resonance (SPR) peak at 544 nm in the UV-Vis spectrum. The characterization of biogenic AuNPs by TEM, EDX and XRD revealed well dispersed spherical particles ranging from 10-40 nm and the presence of elemental gold and its crystalline nature, respectively. The AuNPs showed good stability and the scavenging activity at 50 μg/ml. The in vitro cytotoxicity of biogenic AuNPs against PA-1 and A549 cell lines recorded half maximal inhibitory concentration (IC50) of 45.88 μg/ml and 52.015 μg/ml, respectively.Conclusion: The biogenic AuNPs demonstrated superior antioxidant and antiproliferative activities against cancer cell lines.

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Synthesis, Characterization, and Biological Activity of a Novel Series of Benzo[4,5]imidazo[2,1-b]thiazole Derivatives as Potential Epidermal Growth Factor Receptor Inhibitors

Molecules ◽

10.3390/molecules24040682 ◽

2019 ◽

Vol 24 (4) ◽

pp. 682 ◽

Cited By ~ 3

Author(s):

Xinshan Deng ◽

Xiaoyu Tan ◽

Tiantian An ◽

Qingqing Ma ◽

Zhe Jin ◽

...

Keyword(s):

Antitumor Activity ◽

Cell Lines ◽

Normal Cell ◽

Growth Factor Receptor ◽

Human Cell Line ◽

Thiazole Derivatives ◽

Cellular Toxicity ◽

Epidermal Growth ◽

Inhibitory Activities

Based on the analysis of epidermal growth factor receptor (EGFR) complexes with gefitinib with molecular docking, the scaffold-hopping strategy, combination of the active substructures, and structural optimization of EGFR inhibitors, a novel series of benzo[4,5]imidazo[2,1-b]thiazole derivatives was designed, synthesized, and evaluated for antitumor activity in human cancer cell lines and cellular toxicity against human normal cell lines using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay and EGFR inhibitory activities in vitro. Some target compounds such as 2-(benzo[4,5]imidazo[2,1-b]thiazol-3-yl)-N-(2-hydroxyphenyl)acetamide (D04) and 2-(benzo[4,5]imidazo[2,1-b]thiazol-3-yl)-N-(naphthalen-1-yl)acetamide (D08) have shown significant antitumor activity against the EGFR high-expressed human cell line HeLa. All the target compounds showed hardly any antitumor activity against the EGFR low-expressed human cell line HepG2, and nearly no cellular toxicity against the human normal cell lines HL7702 and human umbilical vein endothelial cell lines (HUVEC). The inhibitory activities against EGFR kinase in vitro of the three target compounds were greatly consistent with the anti-proliferative activities. The preliminary structure–activity relationships of the target compounds were summarized. Conclusively, the novel benzo[4,5]imidazo[2,1-b]thiazole derivatives as novel potential EGFR inhibitors may be used as the potential lead compounds for the development of antitumor agents.

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Protein Precipitation In Vitro as a Measure of Chemical-induced Cytotoxicity: An EDIT Sub-programme

Alternatives to Laboratory Animals ◽

10.1177/026119290102900315 ◽

2001 ◽

Vol 29 (3) ◽

pp. 309-324

Author(s):

Apolonia Novillo ◽

Barbro Ekwall ◽

Argelia Castaño

Keyword(s):

Cell Lines ◽

Human Cell ◽

Exposure Period ◽

Negative Control ◽

In Vitro Cytotoxicity ◽

Protein Precipitation ◽

In Vitro Toxicity ◽

Human Cell Lines ◽

Response Curves

As a priority area of the Evaluation-Guided Development of In Vitro Toxicity and Toxicokinetic Tests (EDIT) programme, an in vitro protein precipitation (PP) assay was used on the 50 reference chemicals of the Multicentre Evaluation of In Vitro Cytotoxicity (MEIC) project, to confirm and extend the MEIC results. Dose–response curves were generated for only 30 of the chemicals, and the concentrations causing 10% (EC10) and 50% (EC50) protein precipitation versus the positive control were chosen as endpoints. The number of chemicals with a positive response increased to 46 when a new endpoint, the minimum effect concentration (MEC) that induces protein precipitation with respect to the negative control, was used. When the results were correlated with in vitro cytotoxicity in human cell lines, a similarly good correlation was found between the various endpoints of the PP assay at 5 hours and the 24-hour IC50 average cytotoxicity in human cell lines, even though the number of chemicals included in the correlation was larger for the MEC. Using the prediction error, the endpoint that gave the best correlation between the PP assay and human cell cytotoxicity was once more found to be the 5-hour MEC, and this was chosen for the PP assay. The sensitivity of the PP assay is lower than that of the in vitro cell-line cytotoxicity assay, possibly due to its shorter exposure period and because precipitation is the ultimate event in the sequence of a protein disturbance. It is expected that earlier denaturation steps would give better sensitivity. However, this simple, inexpensive and rapid assay could be useful in the early stages of testing chemicals.

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Experimental Evidence for Killing the Resistant Cells and Raising the Efficacy and Decreasing the Toxicity of Cytostatics and Irradiation by Mixtures of the Agents of the Passive Antitumor Defense System in the Case of Various Tumor and Normal Cell Lines in Vitro

Cancer Biotherapy & Radiopharmaceuticals ◽

10.1089/cbr.2008.0561 ◽

2009 ◽

Vol 24 (1) ◽

pp. 67-80 ◽

Cited By ~ 3

Author(s):

Gyula Kulcsár

Keyword(s):

Experimental Evidence ◽

Cell Lines ◽

Normal Cell ◽

Defense System ◽

Resistant Cells

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Evaluation of a nonbiologic nanoparticle form of paclitaxel in metastatic pancreatic cancer.

Journal of Clinical Oncology ◽

10.1200/jco.2013.31.15_suppl.e15076 ◽

2013 ◽

Vol 31 (15_suppl) ◽

pp. e15076-e15076 ◽

Cited By ~ 1

Author(s):

Kouros Motamed ◽

Larn Hwang ◽

Chao Hsiao ◽

Vuong N. Trieu

Keyword(s):

Pancreatic Cancer ◽

Phase I ◽

Cell Lines ◽

Advanced Pancreatic Cancer ◽

Metastatic Pancreatic Cancer ◽

In Vitro Toxicity ◽

Pancreatic Cell ◽

Anti Tumor Activity

e15076 Background: The (nab-Pac)/Gemcitabine (Gem) combination has recently been shown to impart a significant survival advantage over Gem alone in patients with metastatic pancreatic cancer. The goal of this study was to define a non-biologic, nanoparticle paclitaxel (NBN-Pac) which has a similar toxicity profile and utilizes the same albumin-mediated transport mechanism. Herein, we report in vitro, preclinical and phase I clinical results for this NBN-Pac in metastatic pancreatic cancer. Methods: In vitro drug cytotoxicity was measured as mean IC50 values following a 72-h exposure in four pancreatic cell lines (MIA Paca-2 and Capan-1 and multi-drug resistant cell lines PANC-1 and ASPC-1). In vivo anti-tumor activities were assessed in xenografted MIA PaCa-2 and PANC-1 models in nude mice treated with three i.v. doses of NBN-Pac (20, 50 mg/kg) and Taxol (20 mg/kg) on days 0, 3, and 6 (q3dx3), and twelve i.v. doses of Gem (140 mg/kg) on every 3 days (q3dx12). A phase I clinical trial (N=18) was conducted to determine the MTD and the recommended phase II dose of the combination therapy with NBN-Pac (220-300 mg/m2, q3w) and Gem (1250 mg/m2) as primary endpoints in first line treatment of subjects with advanced pancreatic cancer. Reduction in the plasma levels of CA19-9 was measured as a PD biomarker. Results: The mean IC50 value of NBN-Pac in four pancreatic cell lines was approximately 30-fold lower than that of Gem. NBN-Pac formulation (50 mg/kg) produced superior anti-tumor activity in the two xenograft models tested over Taxol and Gem at clinically equivalent doses. Our phase I trial established the MTD of this NBN-Pac formulation as 300mg/m2. Moreover, 5 out of 16 subjects (31.3%) were CR or PR with 95% exact confidence interval of (11.0%, 58.7%). The median PFS time was 5.6 month (95% C.I = 2.9). The median OS time could not be estimated as the survival rate did not fall below 50%. Other safety variables revealed no significant abnormality that may have affected the result of the study. Conclusions: NBN-Paclitaxel formulation has superior anti-tumor activity vs. Taxol and Gem in in vitro toxicity assays, preclinical models of pancreatic cancer, as well in a phase I clinical study in patients with advanced pancreatic cancer.

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