scholarly journals Effects of low-pH stress on shell traits of the dove snail, <i>Anachis misera</i>, inhabiting shallow-vent environments off Kueishan Islet, Taiwan

2015 ◽  
Vol 12 (9) ◽  
pp. 2631-2639 ◽  
Author(s):  
Y. J. Chen ◽  
J. Y. Wu ◽  
C. T. A. Chen ◽  
L. L. Liu
Keyword(s):  
Shell Length ◽  
Shell Thickness ◽  
Expression Profiles ◽  
Shell Shape ◽  
Natural Environments ◽  
Additional Information ◽  
Shell Width ◽  
T1 And T2 ◽  
Body Whorl ◽  
Protein Expression Profiles

Abstract. The effects of naturally acidified seawater on shell traits were quantified through the comparison of dove snails (Family: Columbellidae) Anachis misera from vent environments with Euplica sp. from non-vent sites in northeastern Taiwan. Samples of A. misera were collected around a shallow vent (24.8341° N, 121.96191° E), which included the east, south, southwest, and northwest sites. An absence of Anachis snails was found in the most acidic north site (pH 7.19–7.25). Based on the similarities of protein expression profiles, the Anachis snails were classified into two groups, i.e., V-South (pH 7.78–7.82) and V-Rest (pH 7.31–7.83). Comparing their shell traits to the non-vent Euplica sp. from Da-xi (DX) and Geng-fang (GF) (pH 8.1–8.2), a difference in shell shape (shell width : shell length) was found, with the populations having more globular shells than the non-vent ones. The means of shell width were significantly different among sites (p < 0.01), with a descending order of GF > DX > V-South and V-Rest. The relationships of shell length to total weight were curvilinear for both Anachis and Euplica snails. The logarithmically transformed slopes differed significantly among sites, and the mean body weight of the GF population was greater than that of the others (p < 0.01). Positive correlations between shell length and shell thickness of body whorl (T1) and penultimate whorl (T2) were only observed in non-vent GF and DX populations. Anachis snails from vent sites were thinner in T1 and T2 compared to the Euplica snails from non-vent sites (p < 0.05). Within each vent group, shell thickness between T1 and T2 was insignificantly different. Between vent groups, T1 and T2 from V-Rest showed a decrease of 10.6 and 10.2%, respectively, compared to V-South ones. The decrease of T1 and T2 between vent Anachis snails and non-vent Euplica snails was as great as 55.6 and 29.0%, respectively. This was the first study to compare snail's morphological traits under varying shallow-vent stresses with populations previously classified by biochemical responses. Overall, the shallow-vent-based findings provide additional information from subtropics on the effects of acidified seawater on gastropod snails in natural environments.

scholarly journals Effects of low pH stress on shell traits and proteomes of the dove snail, <i>Anachis misera</i> inhabiting shallow vent environments off Kueishan Islet, Taiwan

2014 ◽  
Vol 11 (12) ◽  
pp. 17207-17226 ◽  
Author(s):  
Y. J. Chen ◽  
J. Y. Wu ◽  
C. T. A. Chen ◽  
L. L. Liu
Keyword(s):  
Shell Length ◽  
Size Structure ◽  
Expression Profiles ◽  
Coefficient Of Determination ◽  
Snail Species ◽  
Natural Environments ◽  
The South ◽  
Similar Vein ◽  
Body Whorl ◽  
Protein Expression Profiles

Abstract. The effects of naturally acidified seawater on a snail species, Anachis misera (Family: Columbellidae) were quantified in five shallow vent-based environments off Kueishan Islet, Taiwan. An absence of Anachis snails was observed in the most acidic North site (pH 7.22), and the size structure differed among the remaining East, South, Southwest and Northwest sites. If a positive correlation between shell length and shell width or total weight existed, the coefficient of determination (R2) of the equations was low, i.e., 0.207–0.444. Snails from the Northwest site (pH 7.33) exhibited a more globular shape than those of the South ones (pH 7.80). Standardized shell thickness T1 (thickness of body whorl : shell length) and T2 (thickness of penultimate whorl : shell length) from the Northwest site showed a decrease of 6.3 and 9.4%, respectively, compared to the South ones. In a similar vein, based on the 16 examined protein spots, protein expression profiles of snails in the South were distinct. With further characterization by principle component analysis, the separation was mainly contributed by the first (i.e., spots 8, 1, 15, and 12) and second (i.e., spots 15, 13, 12, 1, and 11) principal-components. As a whole, the shallow vent-based findings provide new information from subtropics on the effects of ocean acidification on gastropod snails in natural environments.

Effect of replacing maize with plantain peels on the performance traits and haematological characteristics of African giant land snail

2022 ◽  
Vol 4 (1) ◽  
pp. 001-006
Author(s):  
Yeigba, B Japhet ◽  
Kpun, IP ◽  
Birigeni, D Charity
Keyword(s):  
Weight Gain ◽  
Blood Cell ◽  
Feed Intake ◽  
Shell Length ◽  
Niger Delta ◽  
Shell Thickness ◽  
Hematological Parameters ◽  
Land Snail ◽  
Land Snails ◽  
Shell Width

The study was carried out at the Niger Delta University, Faculty of Agriculture Teaching and Research Farm, Bayelsa State. A total of Ninety six (96) growing snails (Archachatina marginata). Using Plantain peel as an additive, four diets were formulated at the levels of 0%, 3%, 6%, and 9% to form the treatment diets. The experiment lasted for eight (8) weeks during which the weight gain, feed intake, shell width, shell length, shell thickness and hematological parameters were obtained. The weight gain, shell width, shell length and shell thickness are not significantly different (p<0.05) whereas, the feed intake differed significantly (p<0.05) for all the treatment diets in the experiment. From the results, it was concluded that concentrate with 0% inclusion of Plantain peel meal was suitable for snail diets as it gave the best performance. The hematological parameters measured in the African giant land snails are White blood cell, Neutrophile and Lymphocyte differed significantly (p<0.05). Plantain peel can serve as source of potassium for other animals, as the highest percentage of potassium was found in the hemolymph of snails fed with 9% inclusion of plantain peel. It is recommended that; there should be no inclusion of plantain peels in the diet of the African giant land snails in order to achieve higher weight gain and feed intake.

scholarly journals Prediction Accuracies of Genomic Selection for Nine Commercially Important Traits in the Portuguese Oyster (Crassostrea angulata) Using DArT-Seq Technology

Genes ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 210
Author(s):  
Sang V. Vu ◽  
Cedric Gondro ◽  
Ngoc T. H. Nguyen ◽  
Arthur R. Gilmour ◽  
Rick Tearle ◽  
...  
Keyword(s):  
Moisture Content ◽  
Genomic Selection ◽  
Shell Length ◽  
Prediction Accuracy ◽  
Genomic Information ◽  
Morphometric Traits ◽  
Shell Weight ◽  
Shell Width ◽  
Crassostrea Angulata ◽  
Selection For

Genomic selection has been widely used in terrestrial animals but has had limited application in aquaculture due to relatively high genotyping costs. Genomic information has an important role in improving the prediction accuracy of breeding values, especially for traits that are difficult or expensive to measure. The purposes of this study were to (i) further evaluate the use of genomic information to improve prediction accuracies of breeding values from, (ii) compare different prediction methods (BayesA, BayesCπ and GBLUP) on prediction accuracies in our field data, and (iii) investigate the effects of different SNP marker densities on prediction accuracies of traits in the Portuguese oyster (Crassostrea angulata). The traits studied are all of economic importance and included morphometric traits (shell length, shell width, shell depth, shell weight), edibility traits (tenderness, taste, moisture content), and disease traits (Polydora sp. and Marteilioides chungmuensis). A total of 18,849 single nucleotide polymorphisms were obtained from genotyping by sequencing and used to estimate genetic parameters (heritability and genetic correlation) and the prediction accuracy of genomic selection for these traits. Multi-locus mixed model analysis indicated high estimates of heritability for edibility traits; 0.44 for moisture content, 0.59 for taste, and 0.72 for tenderness. The morphometric traits, shell length, shell width, shell depth and shell weight had estimated genomic heritabilities ranging from 0.28 to 0.55. The genomic heritabilities were relatively low for the disease related traits: Polydora sp. prevalence (0.11) and M. chungmuensis (0.10). Genomic correlations between whole weight and other morphometric traits were from moderate to high and positive (0.58–0.90). However, unfavourably positive genomic correlations were observed between whole weight and the disease traits (0.35–0.37). The genomic best linear unbiased prediction method (GBLUP) showed slightly higher accuracy for the traits studied (0.240–0.794) compared with both BayesA and BayesCπ methods but these differences were not significant. In addition, there is a large potential for using low-density SNP markers for genomic selection in this population at a number of 3000 SNPs. Therefore, there is the prospect to improve morphometric, edibility and disease related traits using genomic information in this species.

SOLVING THE PUZZLE OF THE BIVALVE SHELL FRAGMENT: INFERRING THE ORIGINAL ABUNDANCE AND SIZE FREQUENCY FROM THE FRAGMENTED RECORD

Palaios ◽  
2020 ◽  
Vol 35 (10) ◽  
pp. 432-445
Author(s):  
ERIC N. POWELL
Keyword(s):  
Shell Model ◽  
Shell Length ◽  
Shell Thickness ◽  
Conservative Estimate ◽  
Potential Donor ◽  
Size Frequency ◽  
Shell Fragment ◽  
Length Width ◽  
Bivalve Shell ◽  
Original Size

ABSTRACT Much of the contribution of bivalves to paleoecology is based on the fraction preserved whole. The use of fragments has been limited by the inability to reconstruct the whole shell. For this study, shells of selected species were broken, metrics obtained for shell reconstruction, and a stepwise model developed and tested to evaluate the potential of reconstructing whole shells from fragments. The model is based on the facts that shell thickness is well correlated with shell length, width, and weight, that fragments can be assigned to shell areas, and that each shell area can be matched to a potential donor shell by combinations of length, width, and weight. Simulations using the model show that shell reconstructions are feasible and that the size frequency can be reconstructed more accurately than the number of shells. If all fragments are preserved, shell number tends to be modestly overestimated. Assuming fragment loss, the shell number estimated will be a conservative estimate of original abundance. Reconstructed size frequencies did not differ significantly from the original size frequencies of the whole shells even if 40% of the fragments were not preserved. Biovolume, consequently, may also be estimable. Standardly, bivalve fragments are enumerated by counting intact umbos. If all fragments are preserved, this approach is preferable, as shell model reconstructions overestimate abundance under that circumstance. However, the expectation that fragments with intact umbos are uniquely well preserved is unrealistic and if fragment loss has occurred, relying on all fragments may provide an improved estimate of the original abundance and, in addition, an estimate of the size frequency and the latter proves to be robust even with 40% of the fragments lost.

P-652 Protein expression profiles in non-small cell lung cancer. A tissue microarray-based study of 142 cases

Lung Cancer ◽  
2005 ◽  
Vol 49 ◽  
pp. S290 ◽  
Author(s):  
E. Conde ◽  
R. García Luján ◽  
A. López Encuentra ◽  
L. Sánchez ◽  
M. Sánchez-Céspedes ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Protein Expression ◽  
Small Cell Lung Cancer ◽  
Tissue Microarray ◽  
Cell Lung Cancer ◽  
Expression Profiles ◽  
Small Cell ◽  
Small Cell Lung ◽  
Protein Expression Profiles

scholarly journals Brain death induces the alteration of liver protein expression profiles in rabbits

2014 ◽  
Vol 34 (2) ◽  
pp. 578-584 ◽  
Author(s):  
BING DU ◽  
LING LI ◽  
ZHIBIAO ZHONG ◽  
XIAOLI FAN ◽  
BINGBING QIAO ◽  
...  
Keyword(s):  
Protein Expression ◽  
Brain Death ◽  
Expression Profiles ◽  
Liver Protein ◽  
Protein Expression Profiles

Fluoride exposure inhibits protein expression and enzyme activity in the lung-stage larvae ofAscaris suum

Parasitology ◽  
2006 ◽  
Vol 133 (4) ◽  
pp. 497-508 ◽  
Author(s):  
M. K. ISLAM ◽  
T. MIYOSHI ◽  
M. YAMADA ◽  
M. A. ALIM ◽  
X. HUANG ◽  
...  
Keyword(s):  
Protein Expression ◽  
Expression Profiles ◽  
Immunoblot Analysis ◽  
Specific Protein ◽  
Inorganic Pyrophosphatase ◽  
2D Electrophoresis ◽  
Peptide Mass ◽  
Enzyme Families ◽  
Protein Expression Profiles ◽  
Peptide Mass Spectrometry

Sodium fluoride (NaF) is an anion that has been previously shown to block the moulting process ofAscaris suumlarvae. This study describes moulting and development-specific protein expression profiles ofA. suumlung-stage L3 (AsLL3) following NaF exposure. AsLL3s cultured in the presence or absence of NaF were prepared for protein analysis using two-dimensional (2D) electrophoresis. NaF exposure inhibited at least 22 proteins in AsLL3 compared with moulted larvae (i.e. AsLL4). A further comparison of AsLL4 with those of pre-cultured AsLL3 and NaF-exposed AsLL3 revealed 8 stage-specifically and 4 over-expressed proteins. Immunoblot analysis revealed an inhibition by NaF of 19 immunoreactive proteins. Enzyme assay and immunochemical data showed an inhibition of the moulting-specific inorganic pyrophosphatase activity by 41% and a decreased expression in NaF-treated larvae, indicating its significance in the moulting process. A protein spot associated with NaF inhibition was isolated and identified by peptide mass spectrometry and bioinformatics approaches to be a member of 3–hydroxyacyl–CoA dehydrogenase/short-chain dehydrogenase enzyme families. These results have implications for the identification of proteins specific to the moulting process as potential chemotherapeutic targets.

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