scholarly journals Growth of the coccolithophore <i>Emiliania huxleyi</i> in light- and nutrient-limited reactors: relevance for the BIOSOPE deep ecological niche of coccolithophoresbatch

2016 ◽  
Author(s):  
L. Perrin ◽  
I. Probert ◽  
G. Langer ◽  
G. Aloisi

Abstract. Coccolithophores are unicellular, calcifying marine algae that play an important role in the oceanic carbon cycle via their cellular processes of photosynthesis (a CO2 sink) and calcification (a CO2 source). Alongside the well-known, shallow-water coccolithophore blooms visible from satellites, deep niches of coccolithophores are a poorly known but potentially important coccolithophore ecosystem. We investigated the conditions that regulate the development of a deep coccolithophore niche (150–200 m depth) along the BIOSOPE transect in the South Pacific oceanic gyre. We carried out batch culture experiments with a coccolithophore strain isolated from the BIOSOPE transect, reproducing the in situ conditions of light- and nutrient- (nitrate and phosphate) limitation. By simulating coccolithophore physiology using an internal stores (Droop) physiological model, we were able to constrain fundamental physiological parameters for this BIOSOPE coccolithophore strain. We show that simple batch experiments, in conjunction with physiological modelling, can provide reliable estimates of fundamental physiological parameters that are usually obtained in more time consuming and costly chemostat experiments. The combination of culture experiments, physiological modelling and in situ data from the BIOSOPE cruise show that coccolithophore growth in the deep BIOSOPE niche is co-limited by availability of light and nitrate. This study contributes to the understanding of Emiliania huxleyi physiology, metabolism and behavior in a disadvantageous ecosystem of the ocean.

2016 ◽  
Vol 13 (21) ◽  
pp. 5983-6001 ◽  
Author(s):  
Laura Perrin ◽  
Ian Probert ◽  
Gerald Langer ◽  
Giovanni Aloisi

Abstract. Coccolithophores are unicellular calcifying marine algae that play an important role in the oceanic carbon cycle via their cellular processes of photosynthesis (a CO2 sink) and calcification (a CO2 source). In contrast to the well-studied, surface-water coccolithophore blooms visible from satellites, the lower photic zone is a poorly known but potentially important ecological niche for coccolithophores in terms of primary production and carbon export to the deep ocean. In this study, the physiological responses of an Emiliania huxleyi strain to conditions simulating the deep niche in the oligotrophic gyres along the BIOSOPE transect in the South Pacific Gyre were investigated. We carried out batch culture experiments with an E. huxleyi strain isolated from the BIOSOPE transect, reproducing the in situ conditions of light and nutrient (nitrate and phosphate) limitation. By simulating coccolithophore growth using an internal stores (Droop) model, we were able to constrain fundamental physiological parameters for this E. huxleyi strain. We show that simple batch experiments, in conjunction with physiological modelling, can provide reliable estimates of fundamental physiological parameters for E. huxleyi that are usually obtained experimentally in more time-consuming and costly chemostat experiments. The combination of culture experiments, physiological modelling and in situ data from the BIOSOPE cruise show that E. huxleyi growth in the deep BIOSOPE niche is limited by availability of light and nitrate. This study contributes more widely to the understanding of E. huxleyi physiology and behaviour in a low-light and oligotrophic environment of the ocean.


2019 ◽  
Vol 12 (1) ◽  
pp. 77 ◽  
Author(s):  
Jaime Pitarch ◽  
Marco Bellacicco ◽  
Emanuele Organelli ◽  
Gianluca Volpe ◽  
Simone Colella ◽  
...  

Particulate optical backscattering (bbp) is a crucial parameter for the study of ocean biology and oceanic carbon estimations. In this work, bbp retrieval, by the quasi-analytical algorithm (QAA), is assessed using a large in situ database of matched bbp and remote-sensing reflectance (Rrs). The QAA is also applied to satellite Rrs (ESA OC-CCI project) as well, after their validation against in situ Rrs. Additionally, the effect of Raman Scattering on QAA retrievals is studied. Results show negligible biases above random noise when QAA-derived bbp is compared to in situ bbp. In addition, Rrs from the CCI archive shows good agreement with in situ data. The QAA’s functional form of spectral backscattering slope, as derived from in situ radiometry, is validated. Finally, we show the importance of correcting for Raman Scattering over clear waters prior to semi-analytical retrieval. Overall, this work demonstrates the high efficiency of QAA in the bbp detection in case of both in situ and ocean color data, but it also highlights the necessity to increase the number of observations that are severely under-sampled in respect to others environmental parameters.


2020 ◽  
Vol 158 ◽  
pp. 111392 ◽  
Author(s):  
Vladimir Silkin ◽  
Larisa Pautova ◽  
Mario Giordano ◽  
Marina Kravchishina ◽  
Vladimir Artemiev

Author(s):  
Alexander Myasoedov ◽  
Alexander Myasoedov ◽  
Sergey Azarov ◽  
Sergey Azarov ◽  
Ekaterina Balashova ◽  
...  

Working with satellite data, has long been an issue for users which has often prevented from a wider use of these data because of Volume, Access, Format and Data Combination. The purpose of the Storm Ice Oil Wind Wave Watch System (SIOWS) developed at Satellite Oceanography Laboratory (SOLab) is to solve the main issues encountered with satellite data and to provide users with a fast and flexible tool to select and extract data within massive archives that match exactly its needs or interest improving the efficiency of the monitoring system of geophysical conditions in the Arctic. SIOWS - is a Web GIS, designed to display various satellite, model and in situ data, it uses developed at SOLab storing, processing and visualization technologies for operational and archived data. It allows synergistic analysis of both historical data and monitoring of the current state and dynamics of the "ocean-atmosphere-cryosphere" system in the Arctic region, as well as Arctic system forecasting based on thermodynamic models with satellite data assimilation.


2020 ◽  
Vol 9 (1) ◽  
pp. 64
Author(s):  
Maija Nuppunen-Puputti ◽  
Riikka Kietäväinen ◽  
Lotta Purkamo ◽  
Pauliina Rajala ◽  
Merja Itävaara ◽  
...  

Fungi have an important role in nutrient cycling in most ecosystems on Earth, yet their ecology and functionality in deep continental subsurface remain unknown. Here, we report the first observations of active fungal colonization of mica schist in the deep continental biosphere and the ability of deep subsurface fungi to attach to rock surfaces under in situ conditions in groundwater at 500 and 967 m depth in Precambrian bedrock. We present an in situ subsurface biofilm trap, designed to reveal sessile microbial communities on rock surface in deep continental groundwater, using Outokumpu Deep Drill Hole, in eastern Finland, as a test site. The observed fungal phyla in Outokumpu subsurface were Basidiomycota, Ascomycota, and Mortierellomycota. In addition, significant proportion of the community represented unclassified Fungi. Sessile fungal communities on mica schist surfaces differed from the planktic fungal communities. The main bacterial phyla were Firmicutes, Proteobacteria, and Actinobacteriota. Biofilm formation on rock surfaces is a slow process and our results indicate that fungal and bacterial communities dominate the early surface attachment process, when pristine mineral surfaces are exposed to deep subsurface ecosystems. Various fungi showed statistically significant cross-kingdom correlation with both thiosulfate and sulfate reducing bacteria, e.g., SRB2 with fungi Debaryomyces hansenii.


Materials ◽  
2021 ◽  
Vol 14 (10) ◽  
pp. 2554
Author(s):  
Oleg Naimark ◽  
Vladimir Oborin ◽  
Mikhail Bannikov ◽  
Dmitry Ledon

An experimental methodology was developed for estimating a very high cycle fatigue (VHCF) life of the aluminum alloy AMG-6 subjected to preliminary deformation. The analysis of fatigue damage staging is based on the measurement of elastic modulus decrement according to “in situ” data of nonlinear dynamics of free-end specimen vibrations at the VHCF test. The correlation of fatigue damage staging and fracture surface morphology was studied to establish the scaling properties and kinetic equations for damage localization, “fish-eye” nucleation, and transition to the Paris crack kinetics. These equations, based on empirical parameters related to the structure of the material, allows us to estimate the number of cycles for the nucleation and advance of fatigue crack.


2021 ◽  
Author(s):  
Antonio Pol ◽  
Fabio Gabrieli ◽  
Lorenzo Brezzi

AbstractIn this work, the mechanical response of a steel wire mesh panel against a punching load is studied starting from laboratory test conditions and extending the results to field applications. Wire meshes anchored with bolts and steel plates are extensively used in rockfall protection and slope stabilization. Their performances are evaluated through laboratory tests, but the mechanical constraints, the geometry and the loading conditions may strongly differ from the in situ conditions leading to incorrect estimations of the strength of the mesh. In this work, the discrete element method is used to simulate a wire mesh. After validation of the numerical mesh model against experimental data, the punching behaviour of an anchored mesh panel is investigated in order to obtain a more realistic characterization of the mesh mechanical response in field conditions. The dimension of the punching element, its position, the anchor plate size and the anchor spacing are varied, providing analytical relationships able to predict the panel response in different loading conditions. Furthermore, the mesh panel aspect ratio is analysed showing the existence of an optimal value. The results of this study can provide useful information to practitioners for designing secured drapery systems, as well as for the assessment of their safety conditions.


2020 ◽  
pp. 1-18
Author(s):  
Lander Van Tricht ◽  
Philippe Huybrechts ◽  
Jonas Van Breedam ◽  
Johannes J. Fürst ◽  
Oleg Rybak ◽  
...  

Abstract Glaciers in the Tien Shan mountains contribute considerably to the fresh water used for irrigation, households and energy supply in the dry lowland areas of Kyrgyzstan and its neighbouring countries. To date, reconstructions of the current ice volume and ice thickness distribution remain scarce, and accurate data are largely lacking at the local scale. Here, we present a detailed ice thickness distribution of Ashu-Tor, Bordu, Golubin and Kara-Batkak glaciers derived from radio-echo sounding measurements and modelling. All the ice thickness measurements are used to calibrate three individual models to estimate the ice thickness in inaccessible areas. A cross-validation between modelled and measured ice thickness for a subset of the data is performed to attribute a weight to every model and to assemble a final composite ice thickness distribution for every glacier. Results reveal the thickest ice on Ashu-Tor glacier with values up to 201 ± 12 m. The ice thickness measurements and distributions are also compared with estimates composed without the use of in situ data. These estimates approach the total ice volume well, but local ice thicknesses vary substantially.


2016 ◽  
Vol 16 (14) ◽  
pp. 9435-9455 ◽  
Author(s):  
Matthew J. Alvarado ◽  
Chantelle R. Lonsdale ◽  
Helen L. Macintyre ◽  
Huisheng Bian ◽  
Mian Chin ◽  
...  

Abstract. Accurate modeling of the scattering and absorption of ultraviolet and visible radiation by aerosols is essential for accurate simulations of atmospheric chemistry and climate. Closure studies using in situ measurements of aerosol scattering and absorption can be used to evaluate and improve models of aerosol optical properties without interference from model errors in aerosol emissions, transport, chemistry, or deposition rates. Here we evaluate the ability of four externally mixed, fixed size distribution parameterizations used in global models to simulate submicron aerosol scattering and absorption at three wavelengths using in situ data gathered during the 2008 Arctic Research of the Composition of the Troposphere from Aircraft and Satellites (ARCTAS) campaign. The four models are the NASA Global Modeling Initiative (GMI) Combo model, GEOS-Chem v9-02, the baseline configuration of a version of GEOS-Chem with online radiative transfer calculations (called GC-RT), and the Optical Properties of Aerosol and Clouds (OPAC v3.1) package. We also use the ARCTAS data to perform the first evaluation of the ability of the Aerosol Simulation Program (ASP v2.1) to simulate submicron aerosol scattering and absorption when in situ data on the aerosol size distribution are used, and examine the impact of different mixing rules for black carbon (BC) on the results. We find that the GMI model tends to overestimate submicron scattering and absorption at shorter wavelengths by 10–23 %, and that GMI has smaller absolute mean biases for submicron absorption than OPAC v3.1, GEOS-Chem v9-02, or GC-RT. However, the changes to the density and refractive index of BC in GC-RT improve the simulation of submicron aerosol absorption at all wavelengths relative to GEOS-Chem v9-02. Adding a variable size distribution, as in ASP v2.1, improves model performance for scattering but not for absorption, likely due to the assumption in ASP v2.1 that BC is present at a constant mass fraction throughout the aerosol size distribution. Using a core-shell mixing rule in ASP overestimates aerosol absorption, especially for the fresh biomass burning aerosol measured in ARCTAS-B, suggesting the need for modeling the time-varying mixing states of aerosols in future versions of ASP.


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