Patterns of longer-term climate change effects on CO₂ efflux from biocrusted soils differ from those observed in the short-term

Biological soil crusts (biocrusts) are predicted to be sensitive to the increased temperature and altered precipitation associated with climate change. We assessed the effects of these factors on soil carbon dioxide (CO2) balance in biocrusted soils using a sequence of manipulations over a nine-year period. We warmed biocrusted soils by 2 and, later, by 4 °C to better capture updated forecasts of future temperature, as well as altered monsoon-season precipitation at a site on the Colorado Plateau, USA. Within treatment plots, we used 20 automated flux chambers to monitor net soil exchange (NSE) of CO2 hourly, first in 2006–2007 and then again in 2013–2014, for a total of 39 months. Net CO2 efflux from biocrusted soils in the warming treatment increased a year after the experiment began (2006–2007). However, after 9 years and even greater warming (4 °C), results were more mixed, with a reversal of the increase in 2013 (i.e., controls showed higher net CO2 efflux than treatment plots) and with similarly high rates in all treatments during 2014, a wet year. Over the longer-term, we saw evidence of reduced photosynthetic capacity of the biocrusts in response to both the temperature and altered precipitation treatments. Patterns in biocrusted soil CO2 exchange under experimentally altered climate suggest that (1) warming effects were diminished later in the experiment, even in the face of larger warming and (2) likely drivers of the treatment effects were changes in biocrust species composition and changes in root respiration due to vascular plant responses.