scholarly journals Effect Of Withania Coagulans And Liraglutide On Serum Glp-1, Postprandial And Fasting Blood Glucose In Streptozotocin Induced Diabetic Rats

2019 ◽  
Vol 09 (02) ◽  
pp. 120-123
Author(s):  
Abdul Samad ◽  
Noor Nasir Rajpoot ◽  
Hira Ayaz ◽  
Noman Sadiq
Keyword(s):  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Postprandial Blood Glucose ◽  
Controlled Study ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Withania Coagulans ◽  
Group B ◽  
Experimental Group

Objective: To evaluate effect of Withania coagulans and liraglutide on serum Glucagon like peptide-1, Postprandial and Fasting Blood Glucose levels in streptozotocin induced diabetic rat. Study Design and Setting: This randomize control trile was conducted at Islamic International Medical College in collaboration with National Institute of Health Islamabad. Methodology: This randomized controlled study was performed on a total of forty male Sprague dawly rats, which were initially divided into two groups; Group A (n=10) and Experimental Group (n=30). Diabetes in the Experimental group B was induced by intraperitoneal administration of streptozotocin for 5 days (30mg/kg/day). Diabetes was checked in experimental group by measuring fasting blood glucose (mg/dl) on day 6. Experimental group was further divided into Group B (Diabetic control), Group C (Withania coagulans-treated) and Group D (Liraglutide-treated). Blood sampling was done at day 30 and serum GLP-1, postprandial and fasting blood glucose levels were measured and compared in all groups. Results: Fasting and postprandial blood glucose levels of group C and D were significantly reduced as compared to group B. Serum GLP-1 levels were significantly increased in group C and D as compared to group B. Conclusion: Withania coagulans reduces hyperglycemia in diabetic rats through increasing GLP-1 hormone.

scholarly journals The Effect of Belimbing Wuluh (Averrhoa Bilimbi L.) and Red Guava (Psidium Guajava L.) Extract on Fasting Blood Glucose Levels of Diabetic Wistar Rats Model

2019 ◽  
Vol 5 (2) ◽  
pp. 57
Author(s):  
Yesi Warisman ◽  
Arinda Lironika Suryana ◽  
Zora Olivia
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Wistar Rats ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Control Group ◽  
Standard Diet ◽  
Glucose Levels ◽  
Blood Glucose Levels

Diabetes mellitus is a metabolic disease with special characteristic which increased blood glucose level (hiperglicemic), its happens because of the abnormality of insulin secretion, insulin mechanisme or both of them. Belimbing wuluh and red guava extract can become alternative functional drink that contain rich of vitamin C to help controlling blood glucose level. The aimed of this study was to determined the effect of belimbing wuluh and red guava extract on blood glucose levels of Rattus novergicus strain Wistar diabetic model. This research was an true experimental study with a randomized post-test control group design. Twentyfour (24) male wistar strain rats weighing 200-250 grams, aged 2-3 months, were divided into 4 groups. The control group (K-) non-diabetic rats, the control group (K+) diabetic rats given standard diet, and the treatment group diabetic rats given standard diet and belimbing wuluh and red guava extract intervetion doses 10,7 ml/day (P1) and 21ml/day (P2). Measurement of pretest posttest fasting blood glucose levels was carried out using biolyzer. The data were analyzed by One Way Anova test. The results showed that there were significant differences in delta pretest and posttest fasting blood glucose levels between all groups. Blood glucose levels decreased at 55.6%. In conclusion, belimbing wuluh and red guava extract 21ml/day most effectively decreased fasting blood glucose levels in diabetic wistar rats moldel.   Keywords: belimbing wuluh, blood glucose level, diabetes mellitus, red guava

scholarly journals Comprehensive Evaluation of Anti-hyperglycemic Activity of FractionatedMomordica charantiaSeed Extract in Alloxan-Induced Diabetic Rats

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Shailesh Kumar Choudhary ◽  
Gagan Chhabra ◽  
Dipali Sharma ◽  
Aruna Vashishta ◽  
Sujata Ohri ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Comprehensive Evaluation ◽  
Fasting Blood Glucose ◽  
Protein S ◽  
Protein Band ◽  
Diabetic Rats ◽  
Momordica Charantia ◽  
Size Exclusion ◽  
Glucose Levels ◽  
Blood Glucose Levels

The present study evaluates anti-hyperglycemic activity of fractionatedMomordica charantia(bitter gourd) seed extracts. Fasting blood glucose levels were evaluated before and after administration of different fractions of the seed extract. Among the three fractions tested, fraction Mc-3 (15 mg/kg b.wt.) showed the maximum anti-hyperglycemic activity and reduced blood glucose levels in experimental diabetic rats significantly. The activities of the key regulatory enzymes of glucose metabolism (hexokinase, pyruvate kinase, lactate dehydrogenase, and glucose-6-phosphate dehydrogenase) were determined in Mc-3-treated diabetic animals. Once-daily administration of the fraction Mc-3 for prolonged period of 18 days to the experimental diabetic animals did not result in any nephrotoxicity or hepatotoxicity as evident from insignificant changes in biochemical parameters indicative of liver and kidney functions. Further fractionation of the fraction Mc-3 by size exclusion chromatography resulted in a fraction, designated Mc-3.2, possessing anti-hyperglycemic activity. The fraction Mc-3.2 showed the presence of a predominant protein band of ~11 kDa on SDS-PAGE. Loss in anti-hyperglycemic activity of the Mc-3.2 upon protease treatment indicates the proteinaceous nature of the anti-hyperglycemic principles. Overall, the results suggest thatMomordica charantiaseeds contain an effective anti-hyperglycemic protein(s) which may find application in treatment of diabetes without evident toxic effects.

scholarly journals Evaluation ofMallotus oppositifoliusMethanol Leaf Extract on the Glycaemia and Lipid Peroxidation in Alloxan-Induced Diabetic Rats: A Preliminary Study

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
C. O. Nwaehujor ◽  
I. I. Ezeigbo ◽  
F. C. Nwinyi
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Blood Glucose ◽  
Oral Treatment ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Albino Rats ◽  
Glucose Levels ◽  
Blood Glucose Levels

Objective. Mallotus oppositifolius(Geiseler) Müll. Arg. (Euphorbiaceae) is folklorically used to “treat” diabetic conditions in some parts of Nigeria therefore the study, to investigate the extract of the leaves for activities on hyperglycaemia, lipid peroxidation, and increased cholesterol levelsin vivoin alloxan diabetic rats as well as its potential antioxidant activityin vitro.Methods. Albino rats (240–280 g) were given an injection of 120 mg/kg body weight, i.p. of alloxan monohydrate. After 8 days, diabetic animals with elevated fasting blood glucose levels (>9 mmol/L) were considered and selected for the study.Results. Oral treatment with the extract administered every 12 h by gavage at doses of 100, 200, and 400 mg/kg of the extract to the test rats, for 14 days, resulted in a significant dose-dependent decrease in blood glucose levels from 12.82 ± 1.02 mmol/dL to 4.92 ± 2.01 mmol/dL at the highest dose of 400 mg/kg compared to the control drug and glibenclamide as well as attendant significant decline in diabetic rats employed in the study.Conclusion. The extract also showedin vitroconcentration-dependent antioxidant activity following the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and ferric reducing assays. Findings further suggest the presence of active antidiabetic and antioxidant principles inM. oppositifoliusleaves.

scholarly journals Effect of Sorghum-tigernut Ibyer (A Traditional Gruel) on the Fasting Blood Glucose Levels of Alloxan-induced Diabetic Rats

2019 ◽  
pp. 260-268
Author(s):  
Shalem Shiekuma ◽  
Moses Ukeyima ◽  
Msendoo, Janet Ahuah ◽  
Idoko Blessing ◽  
Terzungwe Tughgba
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Saline Water ◽  
Fasting Blood Glucose ◽  
Sensory Attributes ◽  
Diabetic Rats ◽  
Glucose Levels ◽  
Sorghum Flour ◽  
Blood Glucose Levels ◽  
Alloxan Monohydrate

Background: There is growing interest in the use of natural foods in the management of chronic diseases like diabetes. Ibyer is a fibre rich gruel consumed amongst the Tiv people of Benue State made from whole sorghum or millet flours. Aim: The aim of the study was to evaluate the effect of sorghum-tigernut ibyer on the fasting blood glucose levels and body weight of alloxan monohydrate-induced diabetic rats. Methods: Sorghum flour (SF) and tigernut flour (TNF) were blended at different proportions (100:00; 90:10; 80:20; 70:30) for the purpose of ibyer production. The flour samples were subjected to proximate analysis using standard analytical procedures, the sensory attributes of ibyer produced from the different flour samples was evaluated on a 9-point hedonic scale. Thirty (30) male Wistar rats (100–180 g body weight) were grouped into five (1-5) each group containing six rats. They were induced with diabetes by injecting them with 150ml/kg of body weight with alloxan monohydrate dissolved in saline water (0.9% NaCl) except for group 1. Blood samples were collected from the tail of the rats, prior to induction, 48hrs after induction and 72 hrs after three days of continuous feeding with test diet. Fasting blood glucose was measured using a standard glucometer and test strips. Results: The sensory attributes indicated that ibyer produced from the flour samples were generally acceptable. Fasting blood glucose levels after 72 hrs of feeding were found to be lowered more in groups giving flours with a higher proportion of Tigernut. Conclusion: The results indicated that sorghum-tigernut ibyer exerted hypoglycaemic effect on the experimental animals.

scholarly journals Antidiabetic, Antihyperlipidemic and Renal Protective Activities of Garcinia indica Linn. Clusiaceae

2018 ◽  
Vol 10 (01) ◽  
Author(s):  
Amol Bhalchandra Deore ◽  
Vinayak Dnyandev Sapakal ◽  
Nilofer S. Naikwade
Keyword(s):  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Plasma Lipid ◽  
Ethanol Extract ◽  
Hdl Cholesterol ◽  
Diabetic Rats ◽  
Lipid Levels ◽  
Glucose Levels ◽  
Garcinia Indica ◽  
Blood Glucose Levels

To investigate the antidiabetic, antihyperlipidemic and renal protective activities of the aqueous and ethanol extract of Garcinia indica fruit rinds against alloxan induced diabetes in rats. Wistar rats were made diabetic by a single dose of alloxan hydrate [130 mg/kg i.p.]. After the successful induction of experimental diabetes, rats were divided into five groups each comprising a minimum of six rats. The effects of extracts and glibenclamide on fasting blood glucose, plasma lipid levels and renal profile were examined for 21 days. Blood glucose levels and biochemical parameters such as serum cholesterol, triglycerides, HDL-cholesterol, LDL-cholesterol, urea and creatinine levels of rats were measured using on weekly intervals i.e day 0, 7, 14 and 21 after daily administration of all extracts at dose of 500 mg/kg. Statistical analysis was performed using Dunnett’s test. p less than 0.01 was taken as the criterion of significance. Oral administration of both aqueous and ethanol extract for 21 days caused a significant [p less than 0.01] reduction in blood glucose levels, lipid profile except HDL; urea and creatinine in diabetic rats. Garcinia indica fruit rind possesses antihyperglycemic activity as well improves total lipid levels and renal profile. It can justify folklore uses of the plant in diabetes.

scholarly journals Pengaruh lama mengunyah terhadap kadar glukosa postprandial dewasa obesitas

2020 ◽  
Vol 8 (1) ◽  
pp. 24
Author(s):  
Arin Wulansari ◽  
Fryta Ameilia Luthfinnisa ◽  
Fuadah Uyun ◽  
Dwi Retnoningrum ◽  
Fifin Luthfia Rahmi ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Postprandial Glucose ◽  
Postprandial Blood Glucose ◽  
Glucose Levels ◽  
Glucose Testing ◽  
Blood Glucose Levels ◽  
Cephalic Phase ◽  
Significant Difference ◽  
The Mean

Background: Obesity cause various physiological changes in the body, one of which is insulin resistance causes high blood glucose levels. Chewing is a stimulus of cephalic phase responses and sensory stimulation that can increase hormones releasing such as insulin, ghrelin, cholecystokinin (CCK) and glucagon like peptide-1 (GLP-1). Chewing plays important role in determining postprandial plasma glucose concentration.Objective: Investigate the effect of chewing on postprandial blood glucose in obese adults.Method: This was true experimental research. Research subjects were treated in the form of chewing 22 times and 40 times each mouthful. Blood glucose levels were measured using glucometer on fasting blood glucose and postprandial blood glucose 15 minutes, 30 minutes, 60 minutes, and 120 minutes. Statistical test using Independent t-test.Results: The mean postprandial glucose levels in the 22 chews group at 15 minutes, 30 minutes, 60 minutes, and 120 minutes were 112.11 ± 14.3328, 126.11 ± 15.667, 116.94 ± 15.539, and 89.67 ± 11.668 . While the mean postprandial blood glucose levels in the 40 chews group at 15 minutes, 30 minutes, 60 minutes, and 120 minutes were 122.22 ± 14.381, 129.61 ± 15.112, 109.50 ± 14.995, and 85.83 ± 13.963. There were statistically significant differences between chewing groups 22 times and chewing 40 times on fasting blood glucose and 15 minutes postprandial blood glucose (p = 0.041 and p = 0.042), while on 30 minutes postprandial glucose testing, 60 minutes , and 120 minutes there was no significant difference (p> 0.05).Conclusion: There was significant differences in 15 minutes postprandial blood glucose level between group 22 times chewing and 40 times chewing each mouthful.

scholarly journals Diabetogenic effect of gluten in Wistar albino rats: a preliminary preclinical screening

2020 ◽  
Author(s):  
Robin Shetty ◽  
Mohandas Rai ◽  
Rajan Chandrashekar ◽  
Bhuvanesh Sukhlal Kalal
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Postprandial Blood Glucose ◽  
Albino Rats ◽  
The Public ◽  
Glucose Levels ◽  
Special Diet ◽  
Blood Glucose Levels ◽  
Wistar Albino Rats

Background and objectives. Gluten-related disease affects less than 1% population and is not considered of relevance at the public health level. However, the consumption of a gluten-free diet has been most commonly adopted as a special diet worldwide in the recent past. In the present study, we investigated the association of gluten intake and diabetic Wistar albino rats. Methods. Thirty adult Wistar rats were randomly divided into five groups: control, diabetic, and test treated with pure gluten (100, 200 and 400 mg/kg body weight). Diabetes was induced in rats by intraperitoneal injection of Streptozotocin (65 mg/kg) after a dose of nicotinamide (110 mg/kg). Body weight, fasting blood glucose levels, postprandial blood glucose levels and histopathology of the pancreas were compared. Results. Fasting blood glucose levels and postprandial blood glucose were significantly higher in diabetes animals but there were no significant changes in gluten treated groups. Other parameters were not significantly changed among different groups. Conclusions. Gluten at doses 100 mg/kg, 200 mg/kg and 400 mg/kg is not a diabetogenic diet and hence it needs not be excluded from diet for the prevention and management of Type 2 diabetes mellitus.

scholarly journals Prophylactic Effect of Ethanol Extract of Azadirachta indica Leaf in Streptozotocin-induced Diabetic Rats

2020 ◽  
pp. 15-27
Author(s):  
O. C. Ezeigwe ◽  
C. J. Ononamadu ◽  
M. E. Onuegbu ◽  
O. R. Ikpeogu ◽  
I. C. Agugom ◽  
...  
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Ethanol Extract ◽  
Diabetic Rats ◽  
Control Group ◽  
Prophylactic Effect ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Pre Treatment

Background: Medicinal plants are now becoming indispensable in the treatment and management of many ailments. The unaffordability, unavailability and adverse effects of conventional therapy in the treatment and management of many diseases have geared keen interest in the use of herbal medicine. This work was carried out to investigate the prophylactic effect of the ethanol extract of Azadirachta indica leaf in streptozotocin-induced diabetic rats.   Methods: A total of one hundred (100) rats were randomized into four (4) groups (n=25) and used for the study. Each group of 25 rats was sub-divided into five (5) groups (n=5). The sub-groups comprise: Group A-normal control that was not treated, group B-100 mg/kg body weight of metformin and groups C to E - graded doses (100 mg/kg, 200 mg/kg and 400 mg/kg body weight) of the ethanol leaf extracts of A. indica leaves. The standard drug and the extracts were consecutively administered to groups B-E for 7, 14, 21 and 28 days before the induction of diabetes. Diabetes was induced intraperitoneally using 50 mg/kg bodyweight of streptozotocin. Results: The groups that were administered 100, 200 and 400 mg/kg bw of ethanol extract of A. indica showed a significant (p<0.05) increase in their weight after 21 and 28 days of pre-treatment compared with the control group that was not treated. The graded doses of the extract also have a remarkable effect in the fasting blood glucose levels which was made visible by the significant (p<0.05) reduction recorded in the fasting blood glucose levels compared with the control group that was not pre-treated and the group pre-treated with metformin. Conclusion: The results obtained in this research suggest that ethanol extract of A. indica has the potential to protect against diabetes by delaying its onset. However, the longer the period of pre-treatment, the better the condition of the animals pre-treated as well as the protection as can be seen from the results of the weight and fasting blood glucose levels.

scholarly journals Clinical Update on Optimal Prandial Insulin Dosing Using a Refined Run-to-Run Control Algorithm

2009 ◽  
Vol 3 (3) ◽  
pp. 487-491 ◽  
Author(s):  
Howard Zisser ◽  
Cesar C. Palerm ◽  
Wendy C. Bevier ◽  
Francis J. Doyle ◽  
Lois Jovanovic
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Concentration ◽  
Fasting Blood Glucose ◽  
Glucose Monitoring ◽  
Postprandial Glucose ◽  
Carbohydrate Content ◽  
Postprandial Blood Glucose ◽  
Glucose Levels ◽  
Prandial Insulin ◽  
Blood Glucose Levels

Background: This article provides a clinical update using a novel run-to-run algorithm to optimize prandial insulin dosing based on sparse glucose measurements from the previous day's meals. The objective was to use a refined run-to-run algorithm to calculate prandial insulin-to-carbohydrate ratios (I:CHO) for meals of variable carbohydrate content in subjects with type 1 diabetes (T1DM). Method: The open-labeled, nonrandomized study took place over a 6-week period in a nonprofit research center. Nine subjects with T1DM using continuous subcutaneous insulin infusion participated. Basal insulin rates were optimized using continuous glucose monitoring, with a target fasting blood glucose of 90 mg/dl. Subjects monitored blood glucose concentration at the beginning of the meal and at 60 and 120 minutes after the start of the meal. They were instructed to start meals with blood glucose levels between 70 and 130 mg/dl. Subjects were contacted daily to collect data for the previous 24-hour period and to give them the physician-approved, algorithm-derived I:CHO ratios for the next 24 hours. Subjects calculated the amount of the insulin bolus for each meal based on the corresponding I:CHO and their estimate of the meal's carbohydrate content. One- and 2-hour postprandial glucose concentrations served as the main outcome measures. Results: The mean 1-hour postprandial blood glucose level was 104 ± 19 mg/dl. The 2-hour postprandial levels (96.5 ± 18 mg/dl) approached the preprandial levels (90.1 ± 13 mg/dl). Conclusions: Run-to-run algorithms are able to improve postprandial blood glucose levels in subjects with T1DM.

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