Anti-inflammatory Activity Test for Ethanol Extract Moon Flower (Tithonia diversifolia) Leaves to Male White Mice

Objective: This study aims to investigate the anti-inflammatory effect of the ethanolic extract of Callyspongia sp. using stabilization of the human red blood cell (HRBC) membrane method and its acute toxicity using brine shrimp lethality test (BSLT) method. Methods: Callyspongia sp. was macerated with 96% ethanol. Extract characterized and screened for the secondary metabolite. Anti-inflammatory activity by stabilization of the HRBC membrane method with a varied dose of 50 ppm; 100 ppm; 200 ppm; 400 ppm; 800 ppm; 1600 ppm; and 3200 ppm. Solutions observed using a photometer to describing stability and ability in preventing membranes hemolytic and statistically analyzed using SPSS. Acute toxicity carried out by the BSLT method and analyzed using Minitab®ver. 17.2.1. Results: The phytochemical screening was indicating that Callyspongia sp. contains flavonoid, alkaloid, and terpenoid. The results of the anti-inflammatory activity test showed that the percentage value of stability and hemolysis of extracts with doses of 50, 100, 200, 400, 800, 1600, and 3200 ppm were 55% and 45%, 63% and 37%, 70% and 30%, 74% and 26%, 80% and 20%, 87% and 13%, and 97% and 3%, respectively. It showed that extract of sponge Callyspongia sp. in all varied dose has activity in stabilizing the HRBC membrane thus can be potential as an anti-inflammatory. The results of acute toxicity assay showed that the value of LC50 was 1281.45 μg/ml and categorized as nontoxic to Artemia salina Leach. Conclusion: Various concentrations of Callyspongia sp. effective as an anti-inflammatory in stabilizing HRBC, and categorized as safe.

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Anti-inflammatory Activity of Ethanol Extract of Lotus (Nelumbo nucifera G.) Seed Against White Male Rats Using Paw Edema Method

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v11i4.4918 ◽

2021 ◽

Vol 11 (4) ◽

pp. 1-4

Author(s):

Khairani Fitri ◽

Tetty Noverita Khairani ◽

Kristin Tiurma Sianturi ◽

Leny Leny ◽

Ihsanul Hafiz

Keyword(s):

White Male ◽

Ethanol Extract ◽

Nelumbo Nucifera ◽

Inflammatory Activity ◽

Male Rats ◽

Control Group ◽

Paw Edema ◽

White Rats ◽

Activity Test ◽

Anti Inflammatory

The purpose of this study was to determine the anti-inflammatory activity test of ethanol extract of lotus seeds in rats induced by carrageenan. The research method used was experimental. The object used in this study was male white rats. Observations were made for 6 hours and then the data were analyzed using the one way analysis of variance (ANOVA) test. The anti-inflammatory activity test was carried out on the test animals which were divided into 5 groups, 3 each with the suspension test group with a dose of 200, 300, 400 mg/kg bw, the negative control group was the CMC Na 1% suspension and the positive control was diclofenac Na 2, 25 mg/kg bw. The results showed that the ethanol extract of the seeds of lotus (Nelumbo nucifera G) was proven to have an anti-inflammatory effect on carrageenan-induced male white rats at doses of 200, 300 and 400 mg/kg BW with the percentage value of inflammation inhibition at the 6th hour was 65.79, 74.65 and 80.77%, with the most effective dose in reducing anti-inflammatory drugs being 400 mg/kg bw. The ethanol extract of the seeds of lotus (Nelumbo nucifera) has anti-inflammatory activity. Keywords: anti-inflammatory activity, Nelumbo nucifera, paw edema

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Anti-Inflammation Activity Test of Nanoparticles Ethanol Extract of Temulawak Rhizome (Curcuma xanthorrhiza Roxb.) with Protein Denaturation Inhibition Method

JURNAL ILMU KEFARMASIAN INDONESIA ◽

10.35814/jifi.v16i2.569 ◽

2018 ◽

Vol 16 (2) ◽

pp. 225

Author(s):

Yunahara Farida ◽

Deni Rahmat ◽

Agi Widia Amanda

Keyword(s):

Antiinflammatory Activity ◽

Protein Denaturation ◽

Ethanol Extract ◽

Inflammatory Activity ◽

Ionic Gelation ◽

Activity Test ◽

Curcuma Xanthorrhiza ◽

Anti Inflammatory ◽

Anti Inflammation ◽

Better Than

Javanese turmeric rhizome (Curcuma xanthorrhiza Roxb.) has been known to have many health benefits as antibacterial, anti-inflammatory, antidiabetic and anticancer. The purpose of this study was to compare the anti-inflammatory activity of temulawak rhizome extract with its nanoparticles. Temulawak rhizome extract was obatained by maceration using 96% (v/v) ethanol as a solvent. The extracts were made into nanoparticles by ionic gelation method which used chitosan and tripolyphosphate. The results of antiinflammatory activity test using a method of inhibiting protein denaturation showed the persentage of inhibition of protein denaturation (IC50) of the extract of temulawak rhizome of 521.67±5.80 ppm while the IC50 of the nanoparticles was 398.02±1.78 ppm. Accordingly, the antiinflammatory activity of the nanoparticles was better than the extract of temulawak rhizome.

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Scientific Evaluation of anti-inflammatory potential of Macrotyloma uniflorum seeds extracts

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11ispl4.4393 ◽

2020 ◽

Vol 11 (SPL4) ◽

pp. 1883-1888

Author(s):

Anandarajagopal K ◽

Abdullah Khan ◽

Sugalia S ◽

Bama Menon ◽

Tan Ching Siang ◽

...

Keyword(s):

Antioxidant Properties ◽

Water Extract ◽

Ethanol Extract ◽

Inflammatory Activity ◽

Leguminous Plant ◽

Paw Edema ◽

Reference Drug ◽

Macrotyloma Uniflorum ◽

Phenolic Constituents ◽

Anti Inflammatory

Phytochemicals possessing the antioxidant properties naturally present in food attract a greater interest to healthcare researchers due to their desirable health effects on human health as they can be explored for protection against oxidative deterioration. Macrtotyloma uniflorum is a leguminous plant belonging to the family Fabaceae and commonly known as Horse gram. Aqueous and ethanol extracts of seeds of Macrotyloma uniflorum were evaluated for their anti-inflammatory effects using the scientific protocol on experimental rats. Extraction was carried out using the cold maceration method, and the anti-inflammatory activity was evaluated using a digital plethysmometer in the experimental rats injected with carrageenan to produce paw edema. Preliminary phytochemical studies confirmed the presence of various bioactive compounds such as alkaloids, glycosides, carbohydrates, proteins and amino acids, terpenoids, tannins, and phenolic compounds in both extracts while flavonoids were found only in ethanol extract. Both extracts of M. uniflorum seeds (200 mg/ml) significantly (p<0.01) reduced the paw edema volume induced by carrageenan. The ethanol extract of M. uniflorum seeds exhibited more potent anti-inflammatory activity than water extract, that might be due to the presence of flavonoids in ethanol extract. The activity of the extracts was compared with diclofenac sodium (10mg/kg b.wt.) as a reference drug. From the results, it may be suggested that the antioxidative potential of phenolic constituents and flavonoids is the primary factors for the anti-inflammatory activity of M. uniflorum seeds extracts.

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AKTIVITAS ANTIINFLAMASI EKSTRAK ETANOL 70% DAUN ASHITABA (Angelica keiskei) SECARA IN VIVO DENGAN PENGINDUKSI KARAGENAN

JFL : Jurnal Farmasi Lampung ◽

10.37090/jfl.v7i2.56 ◽

2019 ◽

Author(s):

Haka As'ada ◽

Yardi Saibi ◽

Hendri Aldrat

Keyword(s):

Dose Range ◽

White Male ◽

Ethanol Extract ◽

Negative Control ◽

Inflammatory Activity ◽

Male Rat ◽

Control Group ◽

Angelica Keiskei ◽

Anti Inflammatory

Ashitaba leaves (Angelica keiskei) or also known as tommorow's leaf is plant that known to have various health benefit, one of them is as an anti-inflammatory activity. The anti-inflammatory activity of ashitaba leaves has been known through in vitro assays. This study aims to determine the anti-inflammatory activity of 70% ethanol extract of ashitaba leaves through in vivo assay. Anti-inflammatory activity was performed on white male rat of Sprague dawley strain with induction method of edema on rat's foot using 1% carrageenan 0.2 ml. Rats were divided into 5 groups. The negative control group was given a 0.5% Na-CMC suspension, a positive control group was given sodium diclofenac suspension of 5.14 mg / kgBW, and the test group was given 70% ethanol extract of ashitaba leaves at a dose of 1000; 2000; and 4000 mg / kgBW suspended in 0.5% Na-CMC. The results showed that in that dose range the 70% ethanol extract of ashitaba leaves had anti-inflammatory activity that did not depend on the dose. Percentage of edema of 70% ethanol extract of ashitaba leaves dose 1000; 2000; 4000 mg / kgBB was significantly different with negative control (p ≤ 0,05) and had percentage of edema inhibition respectively 83,95%, 79,01%, and 80,25%. The results of this study showed that 70% ethanol extract of ashitaba leaves have anti-inflammatory activity. Keywords: Ashitaba, Angelica keiskei, tommorow's leaf, anti-inflammatory, carrageenan.

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Topical anti-inflammatory activity test and identification of anti-inflammatory active compounds of ether extract of stem bark and leaves resin of tenggulun protium javanicum burm

International Journal of Current Advanced Research ◽

10.24327/ijcar.2017.2630.0060 ◽

2017 ◽

Vol 6 (3) ◽

pp. 2626-2630

Author(s):

Suirta I.W ◽

◽

Puspawati N.M ◽

Raka Astiti A I.A ◽

Sahara E ◽

...

Keyword(s):

Stem Bark ◽

Inflammatory Activity ◽

Ether Extract ◽

Active Compounds ◽

Activity Test ◽

Anti Inflammatory

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Anti-Inflammatory Activity of Ethanol Extract of Marine Sponge Petrosia Sp. by Supression the Level of Tumor Necrosis Factor-alpha

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00770 ◽

2021 ◽

pp. 4435-4439

Author(s):

Adryan Fristiohady ◽

Muhammad Hajrul Malaka ◽

Andi Rizqa Wahyuni Safitri ◽

Dewo Diha ◽

Saripuddin Saripuddin ◽

...

Keyword(s):

Inflammatory Process ◽

Oral Treatment ◽

Ethanol Extract ◽

The Body ◽

Inflammatory Activity ◽

Inflammatory Agent ◽

Edema Volume ◽

Group I ◽

Tnf Α ◽

Anti Inflammatory

Inflammation is the host's protective response to any stimulus that harms the body. Excessive inflammatory process causes tissue damage. Therefore, an anti-inflammatory agent is needed. The use of natural ingredients, especially sea sponges, is an option to reduce the side effects of anti-inflammatory agents. This utilization is related to the discovery of new agents. So, we tested the effect of the ethanol extract of Petrosia sp. as an anti-inflammatory agent. Animal induced with 1% carrageenan and left for 1 hour. After that the animals were divided into 6 groups (n = 4) and given oral treatment, namely: Group I (normal group); Group II (negative group); Group III (ethanol extract of Petrosia sp. Concentration of 0.05mg/ml); Group IV (ethanol extract of Petrosia sp. Concentration 0.1mg/ml); Group V (ethanol extract of Petrosia sp. Concentration 0.2mg/ml); and Group VI (positive group, Diclofenac Sodium). After 1 hour, the animals were measured for edema volume and plasma TNF-α levels. Based on the research conducted, the ethanol extract of Petrosia sp. decreased edema volume and plasma TNF-α levels in inflammatory mice. The concentration of 0.2mg/mL had a significant effect on the negative control used (p <0.05). On the other hand, Petrosia sp. indicates the presence of alkaloids, flavonoids, and steroids. They may play an important role in the anti-inflammatory process. Thus, it can be concluded that the ethanol extract of Petrosia sp. has anti-inflammatory activity.

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