scholarly journals THE PREVALENCE OF Chlamydia trachomatis AMONGST ASYMPTOMATIC PREGNANT WOMEN IN FOUR LOCAL GOVERNMENT AREAS OF DELTA STATE

2020 ◽  
Vol 1 (2) ◽  
pp. 13-23
Author(s):  
H. T. Bamikole ◽  
I. B. Idemudia ◽  
E. E. Imariagbe ◽  
F. O. Ekhaise
Keyword(s):  
Chlamydia Trachomatis ◽  
Local Government ◽  
Pregnant Women ◽  
Age Groups ◽  
Sexually Active ◽  
Chain Reaction ◽  
Men And Women ◽  
Primary Health ◽  
Significant Difference ◽  
Polymerase Chain

The prevalence of Chlamydia trachomatis among asymptomatic pregnant women in four Local Government Areas (LGAs) in Delta State, Nigeria was investigated in this study. The four LGAs were Ethiope West, Sapele, Warri South and Warri North. A total of 200 pregnant women who visited the Primary Health Care Centres for antenatal care aged between 16 and 45 years were sampled across the four LGAs comprising 50 pregnant women from each of the LGAs between October 2017 and February 2018. The women were categorized into six age groups: 16 – 20, 21 – 25, 26 – 30, 31 – 35, 36 – 40 and 41 – 45. Collected urine samples were examined in the laboratory for the presence of C. trachomatis using sedimentation and microscopy. Polymerase Chain Reaction (PCR) was used to identify the DNA of the isolated bacteria specimens. Results from the four LGAs showed that 93 pregnant women (46.5%) tested positive for C. trachomatis. Prevalence was highest in the subjects from Warri North LGA (27/93) (29.03%), while the least prevalent was the subjects from Warri South (19/93) (20.04%). Women aged 26 – 30 had the highest prevalence (38/93) (40.86%), while prevalence was least in women aged 41 – 45 (3/93) (3.23%). There was no significant difference in number of infected pregnant women in the four Local Government Areas (p>0.05). Findings from this study are important, considering the growing concern of cases of infertility and death of newborn. Thus, there is a need for sexually active men and women to embark on routine check up to ascertain their health status.

Detection of Chlamydia trachomatis in Pregnant Women by the Papanicolaou Technique, Enzyme Immunoassay and Polymerase Chain Reaction

2000 ◽  
Vol 44 (2) ◽  
pp. 114-123 ◽  
Author(s):  
Carmen Adriana Bañuelos Pánuco ◽  
Irma Deleón Rodríguez ◽  
José Tomás Hernández Méndez ◽  
Lydia Alejandra Martínez Guzmán ◽  
David Akle Fierro ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chlamydia Trachomatis ◽  
Pregnant Women ◽  
Enzyme Immunoassay ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Detection of Brucella sp. and Leptospira sp. in dogs using conventional polymerase chain reaction

2014 ◽  
Vol 58 (4) ◽  
pp. 527-531
Author(s):  
Faham Khamesipour ◽  
Abbas Doosti ◽  
Mohsen Fard Emadi ◽  
Babafela Awosile
Keyword(s):  
Polymerase Chain Reaction ◽  
Age Groups ◽  
Conventional Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Blood Samples ◽  
Stray Dogs ◽  
Supplementary Method ◽  
Significant Difference ◽  
Polymerase Chain ◽  
First Time

Abstract The study was conducted to detect Brucella sp. and Leptospira sp. in blood samples of dogs in Isfahan and Shahrekord province in Iran. A total of 94 blood samples were collected from dogs of different breed, age, sex, and dogs’ type (stray or nonstray). The samples were examined using conventional polymerase chain reaction (PCR). Fourteen (14.89%) dogs were positive for Brucella sp. and 18 (19.15%). dogs for Leptospira sp. There were no significant differences between the prevalence of the pathogens, provinces, sex, and age groups (P > 0.05). However, there was a statistically significant difference in prevalence of Brucella sp. and Leptospira sp. between stray and non-stray dogs (P < 0.0001; χ2 = 30.3767). The study also demonstrated that PCR was successfully used for the first time in Iran for the detection of Brucella sp. and Leptospira sp. in blood samples of dogs. Therefore, we recommend the PCR as a supplementary method with other commonly recognised methods (e.g. serological methods) for the diagnosis of subclinical infections with the microorganisms. Strict measures for the control of stray dogs are also highly recommended.

Mycoplasma genitalium infections in Cuba: surveillance of urogenital syndromes, 2014–2015

2018 ◽  
Vol 29 (10) ◽  
pp. 994-998 ◽  
Author(s):  
Brian A Mondeja ◽  
Nadia M Rodríguez ◽  
Orestes Blanco ◽  
Carmen Fernández ◽  
Jørgen S Jensen
Keyword(s):  
Polymerase Chain Reaction ◽  
Reproductive Tract ◽  
Mycoplasma Genitalium ◽  
Reference Laboratory ◽  
Sexually Active ◽  
Chain Reaction ◽  
Transmitted Infection ◽  
Men And Women ◽  
Sexually Transmitted ◽  
Polymerase Chain

Mycoplasma genitalium is an emerging sexually transmitted pathogen implicated in urethritis in men and several inflammatory reproductive tract syndromes in women. The prevalence of M. genitalium infections in Cuban patients with urogenital syndromes is unknown. The aim of this study was to analyse the prevalence of M. genitalium infection in sexually-active Cuban men and women with urogenital syndromes as a part of aetiological surveillance of urogenital syndromes in Cuba. Samples from men and women with urogenital syndromes submitted to the Mycoplasma Reference Laboratory for mycoplasma diagnosis from 1 January 2014 to 1 June 2015 were analysed by polymerase chain reaction (PCR) for detection of M. genitalium. A total of 971 samples were received and processed. Of the patients tested, 5.7% (47/824) of women and 27.9% (41/147) of men were positive for M. genitalium. This paper presents the largest study of M. genitalium infections among Cuban patients with urogenital syndromes and is Cuba’s first M. genitalium survey. We suggest that M. genitalium should be considered in the Cuban sexually transmitted infection management protocols as an important pathogen, particularly in men.

scholarly journals Non-detection of Chlamydia trachomatis infection by polymerase chain reaction in pregnant Iranian women

2012 ◽  
Vol 3 (1) ◽  
pp. 8 ◽  
Author(s):  
Parvin Hassanzadeh ◽  
Hosein Sharifi ◽  
Abdollah Bazargani ◽  
Reza Khashei ◽  
Amir Emami ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Chlamydia Trachomatis ◽  
Pregnant Women ◽  
Sensitivity And Specificity ◽  
Urine Samples ◽  
World Health ◽  
Chain Reaction ◽  
Transmitted Infection ◽  
Polymerase Chain ◽  
Pcr Test

<em>Chlamydia trachomatis</em> is the most common cause of sexually transmitted infection. In 75% of women and 50% of men infection is asymptomatic. According to World Health Organization reports, the number of new genital infections with <em>Chlamydia trachomatis</em> reaches 100 million annually. The sensitivity and specificity of nacid amplification tests are 95% and 99%, respectively. Urine samples can provide a non-invasive method of testing for the detection of <em>Chlamydia trachomatis</em> by polymerase chain reaction (PCR). The aim of this study was to evaluate the prevalence of <em>Chlamydia trachomatis</em> infection in pregnant women referred to a teaching hospital affiliated to Shiraz University of Medical Sciences. Urine samples were obtained from 210 pregnant women and investigated microscopically and macroscopically by urinalysis. Precipitants were also used for DNA extraction and PCR test for detecting <em>Chlamydia trachomatis.</em> Among 210 urine specimens from women aged 15-39 years, none were positive for <em>Chlamydia trachomatis </em>by PCR. In spite of the high sensitivity and specificity of PCR, and the elimination of inhibitory effects on PCR test, no pregnant woman was positive for <em>Chlamydia trachomatis</em>. Here, we suggest that a larger sample should be studied and other sensitive methods could also be used in the future.

Comparison of laboratory diagnostic methods for urogenital infections caused by Chlamydia trachomatis

2021 ◽  
Vol 50 (4) ◽  
pp. 77-82
Author(s):  
K. V. Shalepo ◽  
E. V. Shipitsina ◽  
A. M. Savicheva ◽  
M. Domeyka
Keyword(s):  
Chlamydia Trachomatis ◽  
Chlamydial Infection ◽  
Diagnostic Methods ◽  
Direct Immunofluorescence ◽  
Chain Reaction ◽  
Men And Women ◽  
Urogenital Infections ◽  
Group I ◽  
High Prevalence ◽  
Polymerase Chain

According to the Russian-Swedish project there was performed a comparison of methods used for Chlamydia trachomatis detection in cervical samples, obtained from 397women and urethral samples from 253 men. All specimens were examined by direct immunofluorescence (DIF), polymerase chain reaction (PCR) and cell culture (CC). In high-prevalence group (group I) chlamydiae were detected in 17,8% and 28,0% of cases in men and women, respectively. Ingroup II containing patients who were subjected to screening examination, chlamydiae were found in 5% of cases both in men and women. PCR was shown to be the most sensitive when cervical samples in group I and cervical and urethral samples in group II were investigated. When urethral samples in group I were tested, DIF proved to have the highest sensitivity. All the methods used were found to be high specific. The search for standards of genital chlamydial infection diagnosis is in progress.

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