Liquid Chromatographic Separations Using Rubber as the Stationary Phase

1963 ◽  
Vol 36 (1) ◽  
pp. 310-312
Author(s):  
P. I. Brewer
Keyword(s):  
Molecular Weight ◽  
Stationary Phase ◽  
Mobile Phase ◽  
Chromatographic Method ◽  
Mineral Oil ◽  
Low Molecular Weight ◽  
Chromatographic Separations ◽  
Liquid Chromatographic Method ◽  
Crosslinked Polystyrene ◽  
Liquid Chromatographic

Abstract In a previous communication it was reported that certain polymers could be separated from mineral oil by a liquid chromatographic method. n-Heptane was used as the mobile phase and small pieces of thin vulcanized natural latex as the stationary phase. It has now been found that compounds of lower molecular weight can also be separated by this method. The use of rubber for the separation of compounds of low molecular weight was first described by Boldingh; he found it advantageous first to swell the rubber with a different solvent from that used as the mobile phase. The use of a crosslinked dextran (“Sephadex”) for separating compounds in aqueous solution has been described by Gelotte. Vaughan has mentioned the possibility of using crosslinked polystyrene beads for this purpose.

A Green Liquid Chromatographic Method For The Simultaneous Determination of Chlorpheniramine Maleate, Pseudoephedrine Hydrochloride and Propyphenazone

2019 ◽  
Vol 15 (6) ◽  
pp. 635-641
Author(s):  
Nadia M. Mostafa ◽  
Ghada M. Elsayed ◽  
Nagiba Y. Hassan ◽  
Dina A. El Mously
Keyword(s):  
Simultaneous Determination ◽  
Mobile Phase ◽  
Dosage Form ◽  
Chromatographic Method ◽  
Green Analytical Chemistry ◽  
Chlorpheniramine Maleate ◽  
Accuracy And Precision ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Background:The concept of green analytical chemistry prevails due to the growing environmental pollution.Objective:Our attempts are to develop simple and eco-friendly method which is non-harmful to the environment by producing minimal waste. In this context, a green liquid chromatographic method was applied for the simultaneous determination of chlorpheniramine maleate, pseudoephedrine hydrochloride and propyphenazone in their combined dosage form.Methods:Separation was carried out using X select HSS RP C18 analytical column (250 × 4.6 mm, 5μm) using methanol - 0.02 M phosphate buffer pH 3 - triethylamine (60:40: 0.1, by volume) as a mobile phase. The separated peaks were detected at 215 nm at a flow rate 1.0 mL/min.Results:Quantification was done over the concentration ranges of 1-25 µg/mL for chlorpheniramine maleate, 5-35 µg/mL for pseudoephedrine hydrochloride and 10-120 µg/mL for propyphenazone. The suggested method was validated with regard to linearity, accuracy and precision according to the International Conference on Harmonization guidelines with good results.Conclusion:It could be used as a safer alternative for routine analysis of the mentioned drugs in quality control laboratories.

scholarly journals BIOEQUIVALENCE STUDIES OF MARKETED CLOZAPINE TABLETS BY USING OPTIMIZED VALIDATED LIQUID CHROMATOGRAPHIC METHOD

2018 ◽  
Vol 10 (3) ◽  
pp. 47
Author(s):  
Udayasree Konikuru ◽  
Rajavel P. ◽  
Vijitha S.
Keyword(s):  
Mobile Phase ◽  
Chromatographic Method ◽  
Accurate Method ◽  
Stability Studies ◽  
Column Temperature ◽  
Rp Hplc ◽  
Liquid Chromatographic Method ◽  
Dissolution Apparatus ◽  
Liquid Chromatographic ◽  
Interday Precision

Objective: A simple, selective, precise and accurate method was developed for the estimation of Clozapine by RP-HPLC technique.Methods: Chromatographic conditions used are stationary phase, Phenomenex BDS (150 mm x 4.6 mm, 5µ), Mobile phase was methanol and water in (80:20) ratio and flow rate was maintained at 1.0 ml/min, column temperature was set at 25 °C, detection wavelength was 240 nm, and diluent was mobile phase. These conditions were finalized for the optimized method.Results: Linearity study was carried out between 10-60 µg/ml, the R2 value was found to be as 0.995. Precision was found to be as follows for system precision 1.052, method precision 1.662, and intraday precision 1.02 and for interday precision 0.93. The % Recovery was found to be 98.60%. LOD and LOQ were found to be 2.7 µg/ml and 8.4 µg/ml respectively. By using the above method assay of the marketed formulation was carried out and the % purity was found to be 99.28 %. Stability studies of Clozapine were done, in all conditions degradation was found to be within the acceptable range.Conclusion: The current validated method was finally applied in bioequivalence studies of four different brands of Clozapine by using dissolution apparatus and percentage drug release was found to be 99.48%, it was within the acceptable limit (NLT 85 %) as per USP.

Validation of Liquid Chromatographic Method for Assay of Calcitriol and Alfacalcidol in Capsule Formulations

1986 ◽  
Vol 69 (6) ◽  
pp. 1026-1030
Author(s):  
Bruce C Flann ◽  
Bruce A Lodge
Keyword(s):  
Standard Deviation ◽  
Calibration Curve ◽  
Mobile Phase ◽  
Chromatographic Method ◽  
Liquid Chromatographic Method ◽  
Chromatographic Procedure ◽  
Liquid Chromatographic ◽  
Better Than

Abstract The validation of a liquid chromatographic procedure suitable for the determination of calcitriol and alfacalcidol in their respective formulations labeled to contain at least 0.25 μ.g drug per unit is described. The capsule content is diluted and chromatographed in 15-20 min on silica columns (5 μm) with a mobile phase of hexane-tetrahydrofuranmethylene dichloride-isopropanol (72 + 12 + 12 + 4, v/v) with detection at 254 nm. The calibration curve is linear. Recoveries of “spikes” averaged 101% with a standard deviation of 2%. Precision was better than 1.5%.

scholarly journals Development and Validation of a Liquid Chromatographic Method for the Simultaneous Determination of Estradiol, Estriol, Estrone, and Progesterone in Pharmaceutical Preparations

2009 ◽  
Vol 92 (3) ◽  
pp. 846-854 ◽  
Author(s):  
Phyllis Wilson
Keyword(s):  
Mobile Phase ◽  
Chromatographic Method ◽  
Pharmaceutical Preparations ◽  
Men And Women ◽  
Naturally Occurring ◽  
Vital Functions ◽  
Liquid Chromatographic Method ◽  
Development And Validation ◽  
Liquid Chromatographic

Abstract Progesterone and estrogens are hormones produced in the human body that are essential for regulating many vital functions. The three major estrogens produced by women are estriol, estradiol, and estrone. Progesterone is a naturally occurring hormone in both men and women. Pharmaceuticals containing estrogens alone or estrogens in combination with progesterone are commonly used in therapy. Patients requiring unique combinations of the drugs rely on pharmacies to compound the ingredients. In order to assess the potency of drugs containing combinations of estrogens and progesterone, a method was developed to determine all four ingredients simultaneously. The liquid chromatographic method utilized a Bondapak C18 column with an isocratic mobile phase of acetonitrilewater (50 + 50, v/v) at a flow rate of 1.0 mL/min and temperature of 30C. Under these conditions, the order of elution was estriol, estradiol, and estrone, followed by progesterone. UV detection was at 205 nm to monitor elution of the estrogens, then switched to 270 nm to monitor progesterone. The method was applied to the analysis of pharmacy-compounded drugs containing combinations of the hormones. Validation studies demonstrated that the method is accurate and precise.

Determination of Cymiazole Residues in Honey by Liquid Chromatography

1993 ◽  
Vol 76 (1) ◽  
pp. 92-94 ◽  
Author(s):  
Paolo Cabras ◽  
Marinella Melis ◽  
Lorenzo Spanedda
Keyword(s):  
Liquid Chromatography ◽  
Chromatographic Analysis ◽  
Mobile Phase ◽  
Chromatographic Method ◽  
Reversed Phase ◽  
Limit Of Determination ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic ◽  
Control Samples

Abstract A liquid chromatographic method is described for the determination of cymiazole residues in honey. This acaricide is determined on a reversed-phase (C18) column, with a CH3CN-O.OOIN HCI-NaCI mixture (950 mL + 50 mL + 0.3 g/L) as the mobile phase, and UV detection at 265 nm. Cymiazole is extracted with n-hexane from aqueous alkalinized (pH 9) honey solutions. No further cleanup of the honey extract was required before chromatographic analysis. Recoveries on control samples fortified with 0.01,0.10, and 1.00 ppm cymiazole ranged from 92 to 102%. The limit of determination was 0.01 ppm.

scholarly journals Biological Assay and Liquid Chromatographic Method for Analysis of Moxifloxacin in Tablets

2005 ◽  
Vol 88 (4) ◽  
pp. 1086-1092 ◽  
Author(s):  
Fanny L B Guerra ◽  
Clesio S Paim ◽  
Martin Steppe ◽  
Elfrides E S Schapoval
Keyword(s):  
Mobile Phase ◽  
Chromatographic Method ◽  
Micrococcus Luteus ◽  
Microbiological Method ◽  
Test Microorganism ◽  
Liquid Chromatographic Method ◽  
Agar Diffusion Assay ◽  
Liquid Chromatographic ◽  
Diffusion Assay ◽  
Interday Precision

Abstract A microbiological assay and a liquid chromatographic method were validated for quantitation of moxifloxacin in tablets. The microbiological method consisted of a cylinder-plate agar diffusion assay using Micrococcus luteus ATCC 9341 as the test microorganism and phosphate buffer (0.1M, pH 8.0) as the diluent solution. The response graphs for standard and sample solutions were linear (r = 0.9479), and no parallelism deviations were detected in the tested levels of concentration (4.0, 8.0, and 16.0 μg/mL). The interday precision was 2.73%. Recovery values were between 96.25 and 100.5%. The chromatographic analyses were performed using a Shim-pack CLC-ODS column (250 × 4.6 mm, 5 μm) with a mobile phase consisting of (A) a mixture of phosphoric acid (0.17%, v/v) with tetramethylammonium hydroxide (0.05M) and acetonitrile (95 + 5, v/v) and (B) methanol (55 + 45, v/v) adjusted to pH 3.0. The flow rate was 1.0 mL/min, and detection was made at 294 nm. The method was linear in a range from 12.0 to 42 μg/mL (r = 0.9999), and the interday precision was 1.39%. Recovery ranged between 101.9 and 103. 81%. Both validated methods were used to quantify the moxifloxacin content in tablets exposed to ultraviolet radiation, and similar results were obtained.

scholarly journals Determination of Parthenolide in Selected Feverfew Products by Liquid Chromatography

2000 ◽  
Vol 83 (4) ◽  
pp. 789-792 ◽  
Author(s):  
Ehab A Abourashed ◽  
Ikhlas A Khan
Keyword(s):  
United States ◽  
Quality Control ◽  
Mobile Phase ◽  
Chromatographic Method ◽  
The United States ◽  
Linear Gradient ◽  
Tanacetum Parthenium ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Abstract The migraine prophylactic herb feverfew (Tanacetum parthenium L.) is marketed in the United States in a variety of forms and compositions. Although its therapeutic efficacy is still uncertain, the sesquiterpene lactone parthenolide is the constituent recommended to be measured for quality control of feverfew preparations. A validated liquid chromatographic method was developed and used to estimate parthenolide in a number of U.S. feverfew market products formulated as capsules, tablets, or crude powder. The method uses a Lichrosphere 5 C18 column, a mobile phase consisting of 50mM NaH2PO4 in H2O (solvent A), and CH3CN–MeOH (90 + 10, v/v; solvent B). Elution was run at a flow rate of 1.0 mL/min with a linear gradient of 50–15% A in B over 20 min and UV detection at 210 nm. The correlation coefficient for the calibration curve was 0.9999 over the range of 0.00–0.400 mg/mL. Overall recovery of parthenolide was 103.1%.

Selektive und sensitive Bestimmung von Nicergolin und Metaboliten in biologischen Medien durch HPLC zur Erforschung seines humanpharmakologischen Verhaltens / Selective and Sensitive Quantification of Nicergoline and Metabolites in Biologie Fluids by HPLC and its Application to Pharmacokinetic Research in Human

1987 ◽  
Vol 42 (9) ◽  
pp. 1187-1194 ◽  
Author(s):  
Michael Nieder ◽  
Halvor Jaeger
Keyword(s):  
Ternary Mixture ◽  
Mobile Phase ◽  
High Performance ◽  
Chromatographic Method ◽  
Plasma Concentrations ◽  
High Performance Liquid Chromatographic ◽  
Ph Adjustment ◽  
Liquid Chromatographic Method ◽  
Trace Concentrations ◽  
Liquid Chromatographic

A high performance liquid chromatographic method was developed to study the pharmacokinetics of nicergoline and its two main m etabolites in humans. The analytes are extracted from blood, plasma and urine into a mixture of chloroform and ether, which is optimised in terms of recovery and absence of interferences. The analytes are separated on a small bore column filled with octadecylsilica. the mobile phase was a ternary mixture of water, acetonitrile and tetrahydrofurane with diisopropylamine for pH adjustment. To maintain the chromatographic stability. the mobile phase is circulated and a saturator column inserted between pump and sampler. Nicergoline is very unstable in blood, it could not be determined in human body fluids after a single dose of 10 mg nicergoline. Metabolite 1 reaches plasma concentrations of about 2 ng/ml after 40 min. metabolite 2 gains to about 8 ng/ml after 4 h. 67% of the dosed nicergoline are excreted in urine as free metabolite 2. free metabolite 1 occurred only in trace concentrations. 18.5% of the dosed drug were excreted as conjugated metabolite 2. 1.1% as conjugated metabolite 1.

Liquid Chromatographic Method for Simultaneous estimation of Thiocolchicoside and Etoricoxib from Tablet formulation

2021 ◽  
pp. 118-122
Author(s):  
Chaitanya A. Gulhane ◽  
Wrushali A. Panchale ◽  
Jagdish V. Manwar ◽  
Ravindra L. Bakal
Keyword(s):  
Mobile Phase ◽  
Chromatographic Method ◽  
Orthophosphoric Acid ◽  
Tablet Formulation ◽  
Simultaneous Estimation ◽  
Rp Hplc ◽  
Ich Guidelines ◽  
Liquid Chromatographic Method ◽  
Percent Recovery ◽  
Liquid Chromatographic

A new simple, reproducible and efficient liquid chromatographic method (RP-HPLC) was developed for simultaneous estimation of thiocolchicoside and etoricoxib for tablet formulation. Formulation containing thiocolchicoside and etoricoxib is used as analgesic. Separation was achieved by Nucleosil (4.6mm I.D × 250 mm) C18 column with mobile phase consist of Acetonitrile: water (0.05% Orthophosphoric acid V/V) in the ratio 25:75 at flow rate 0.7ml . The detection was carried out at 220nm. The retention time of thiocolchicoside and etoricoxib was found to be 3.75 min and 6.13 min respectively. Linearity of THC and ETR was found to be in the range of 2-10µg/mL and 30-150µg/mL. Percent recovery obtained for THC and ETR were 99.98% and 99.69% respectively. The method was validated as per ICH guidelines.

Sign in / Sign up

Export Citation Format

Share Document