scholarly journals Avaliação da compatibilidade in vitro de microrganismos (Bacillus subtilis, Pseudomonas fluorescens e Trichoderma atroviride) com fungicidas comerciais, para a produção sustentável de amendoim

2021 ◽  
Vol 2 (edesp2) ◽  
pp. e21112
Author(s):  
Dean Fernandez ◽  
Matias Torassa ◽  
Maria Alejandra Pérez
Keyword(s):  
Bacillus Subtilis ◽  
Pseudomonas Fluorescens ◽  
Trichoderma Atroviride

A aplicação de fungicidas na semente de amendoim é uma necessidade na semeadura, porém a aplicação combinada com produtos biológicos pode reduzir os efeitos do uso de agroquímicos, favorecendo a qualidade do grão como alimento. O objetivo deste trabalho foi avaliar a compatibilidade in vitro de microrganismos (Bacillus subtilis, Pseudomonas fluorescens e Trichoderma atroviride) com fungicidas comerciais para a produção sustentável de amendoim. Os testes de compatibilidade foram realizados no Laboratório do Centro de Transferência de Qualidade Agroalimentar (FCA UNC). Foram avaliados três combinações de fungicidas comerciais disponíveis no setor de amendoim (F1, F2, F3), em diferentes doses (0, 30, 50, 100 e 1000 mg.l-1). Em placas de petri com meio APG com cada fungicida, foram inseridos B. subtilis, P. fluorescens e T. atroviride, que foram mantidos em câmaras com condições controladas. Sete dias após a semeadura, o crescimento bacteriano foi avaliado de acordo com a escala de compatibilidade e para Trichoderma foi calculada a porcentagem de inibição do crescimento radial (PICR). O delineamento foi completamente aleatório; para cada biológico, foram avaliadas três repetições para cada fungicida e dose. Todo o teste foi repetido três vezes. Os dados foram submetidos à análise de variância e os valores médios do PICR foram comparados por Tukey (p<0,05). Os resultados mostraram um efeito diferencial dos fungicidas sobre os biológicos. F1 (Tiabendazol + Fludioxonil + Metalaxil-M + Azoxistrobina) pode ser combinado com P. fluorescens em todas as doses e B. subtilis em doses não superiores a 50 mg.l-1. F2 (Ipconazol + Metalaxil) e F3 (Ipconazol + Metalaxil M + Carboxina) podem ser aplicados com P. fluorescens mesmo em altas concentrações. Apenas F3 pode ser combinado com T. atroviride até 100 mg.l-1. A combinação correta de fungicidas e produtos biológicos contribuirá efetivamente para a sustentabilidade do sistema de produção de amendoim.

scholarly journals Cultivar Contributes to the Beneficial Effects of Bacillus subtilis PTA-271 and Trichoderma atroviride SC1 to Protect Grapevine Against Neofusicoccum parvum

2021 ◽  
Vol 12 ◽  
Author(s):  
Catarina Leal ◽  
Nicolas Richet ◽  
Jean-François Guise ◽  
David Gramaje ◽  
Josep Armengol ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Trichoderma Atroviride ◽  
In Planta ◽  
Neofusicoccum Parvum ◽  
Beneficial Effects ◽  
Grapevine Trunk Diseases ◽  
Mutual Antagonism ◽  
Direct Benefits ◽  
Botryosphaeria Dieback

Grapevine trunk diseases (GTDs) are a big threat for global viticulture. Without effective chemicals, biocontrol strategies are developed as alternatives to better cope with environmental concerns. A combination of biological control agents (BCAs) could even improve sustainable disease management through complementary ways of protection. In this study, we evaluated the combination of Bacillus subtilis (Bs) PTA-271 and Trichoderma atroviride (Ta) SC1 for the protection of Chardonnay and Tempranillo rootlings against Neofusicoccum parvum Bt67, an aggressive pathogen associated to Botryosphaeria dieback (BD). Indirect benefits offered by each BCA and their combination were then characterized in planta, as well as their direct benefits in vitro. Results provide evidence that (1) the cultivar contributes to the beneficial effects of Bs PTA-271 and Ta SC1 against N. parvum, and that (2) the in vitro BCA mutual antagonism switches to the strongest fungistatic effect toward Np-Bt67 in a three-way confrontation test. We also report for the first time the beneficial potential of a combination of BCA against Np-Bt67 especially in Tempranillo. Our findings highlight a common feature for both cultivars: salicylic acid (SA)-dependent defenses were strongly decreased in plants protected by the BCA, in contrast with symptomatic ones. We thus suggest that (1) the high basal expression of SA-dependent defenses in Tempranillo explains its highest susceptibility to N. parvum, and that (2) the cultivar-specific responses to the beneficial Bs PTA-271 and Ta SC1 remain to be further investigated.

scholarly journals Rizobacterias con potencial antagonista in vitro a Mycosphaerella fijiensis Morelet

2020 ◽  
Vol 13 (2) ◽  
Author(s):  
Karen Tatiana Chávez Arteaga ◽  
Jefferson Javier Guato Molina ◽  
Jorge Luis Rodríguez Acosta ◽  
Ángel Virgilio Cedeño Moreira ◽  
Ricardo Fernando Romero Meza ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Pseudomonas Putida ◽  
Serratia Marcescens ◽  
Pseudomonas Fluorescens ◽  
Mycosphaerella Fijiensis ◽  
Subtilis Atcc ◽  
Bacillus Subtilis Atcc ◽  
Pseudomonas Veronii ◽  
Enterobacter Asburiae

El empleo de bio-controladores en la agricultura beneficia los aspectos fisiológicos en plantas, a diferencia de la constante aplicación de pesticidas en el cultivo del banano ha ocasionado la pérdida de la sensibilidad en M. fijiensis, reduciendo la microbiota del suelo. El objetivo se enfocó en caracterizar el potencial antagónico de las PGPR en inhibición de germinación de ascósporas y desarrollo micelial de M. fijiensis. Se realizaron cultivos monospóricos de M. fijiensis e identificado por PCR. Se evaluaron los extractos celulares de Pseudomonas putida PB3-6, Klebsiella variicola BO3-4, Enterobacter asburiae BA4-19, Serratia marcescens PM3-8, Enterobacter asburiae PM3-14, Pseudomonas protegens CHA0, Pseudomonas fluorescens WCS417, Pseudomonas veronii R4 y Bacillus subtilis ATCC 5540 para sus evaluaciones antagonistas: a) Inhibición del tubo germinativo de las ascósporas al 2% y b) Desarrollo micelial al (2 y 10 %). La PCR empleado en la identificación de M. fijiensis se confirma el producto de amplificación de 1018 pb. El factor antagónico de los extractos celulares al 2 % de PM3-14 y CHA0 inhibe sobre el 80 % al desarrollo de los tubos germinativos. La inhibición al desarrollo micelial del extracto celular al 2 %, de CHA0 logró una efectividad del 54 % y las cepas (PM3-8, PM3-14 y BA4-19) con (32, 26 y 26 %). Al 10 % del extracto de la cepa PM3-8 inhibe el desarrollo micelial con niveles de turbidez de 0,47 (OD600nm). El empleo de estos bio-controladores en la agricultura ofrecerá una alternativa para beneficiar en la reducción del uso de agroquímicos

In vitro evaluation of bio-agents against Pyricularia oryzae (Cav.) causing rice blast disease

2017 ◽  
Vol 37 (03) ◽  
Author(s):  
Akhilesh Kumar Kulmitra ◽  
Neha Sahu ◽  
V.B. Sanath Kumar ◽  
Thejesha A. G. ◽  
Amlan Ghosh ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Pseudomonas Fluorescens ◽  
Rice Blast ◽  
Mycelial Growth ◽  
Trichoderma Viride ◽  
Culture Technique ◽  
Pyricularia Oryzae ◽  
Blast Disease ◽  
Dual Culture

The five different bio-agents viz., Trichoderma viride, T. harzianum, T. virens, Pseudomonas fluorescens and Bacillus subtilis were evaluated against Pyricularia oryzae at four and eight days after incubation through dual culture technique. Among the five different bio-agents, highest per cent inhibition of mycelial growth of fungus was recorded in T. virens i.e. 67 per cent and 70 percent after four and eight days after incubation respectively with mean of 68.5 per cent followed by Trichoderma viride with the inhibition of 61 and 63 per cent respectively with mean of 62 per cent. The Pseudomonas fluorescens did not show any inhibition of mycelial growth of P. oryzae as the pathogen over grew the bio-agents.

scholarly journals  Management of bacterial blight of cotton using a mixture of Pseudomonas fluorescens and Bacillus subtilis

2010 ◽  
Vol 46 (No. 2) ◽  
pp. 41-50 ◽  
Author(s):  
K. Salaheddin ◽  
V. Valluvaparidasan ◽  
D. Ladhalakshmi ◽  
R. Velazhahan
Keyword(s):  
Bacillus Subtilis ◽  
Pseudomonas Fluorescens ◽  
Bacterial Blight ◽  
Maximum Yield ◽  
Biochemical Properties ◽  
Field Conditions ◽  
Phenotypic Characteristics ◽  
Polymerase Chain ◽  
Number Of Branches

The potential of antagonistic rhizobacteria in the management of bacterial blight of cotton caused by Xanthomonas axonopodis pv. malvacearum (Xam) was evaluated under greenhouse and field conditions. In this study, 93 bacterial isolates from the rhizosphere of cotton were screened for their efficacy in inhibiting the growth of Xam in vitro. Among them, 21 isolates were found to inhibit the in vitro growth of Xam. These isolates were identified as Pseudomonas fluorescens and Bacillus subtilis based on phenotypic characteristics, biochemical properties and using 16S-23S intergenic transcribed spacer-Polymerase Chain Reaction (PCR). Among the 21 isolates, the isolates P. fluorescens Pf32 and P. fluorescens Pf93 and B. subtilis B49 exhibited the maximum inhibitory activity against Xam. Talc-based powder formulations of the effective antagonistic isolates of P. fluorescens (Pf32, Pf93) and B. subtilis (B49) were developed and evaluated individually and in combination for their efficacy in the management of bacterial blight of cotton under greenhouse and field conditions. The P. fluorescens isolates Pf32 and Pf93 and Bacillus subtilis isolate B49 survived well in the talc-based formulation for more than 90 days. The application of a mixture of Pf32, Pf93 and B49 to seed, soil and foliage significantly reduced the bacterial blight incidence and increased the plant height, number of branches and number of bolls under field conditions. The plots treated with a mixture of Pf32, Pf93 and B49 recorded the maximum yield of 1915 kg/ha and 1512 kg/ha in trial I and trial II compared to 1210 kg/ha and 987 kg/ha in the untreated control, respectively.

scholarly journals Penapisan Beberapa Isolat Pseudomonas fluorescens, Bacillus subtilis dan Trichoderma harzianum yang bersifat Antagonistik terhadap Ralstonia solanacearum pada Tanaman Kentang

Agrikultura ◽  
2009 ◽  
Vol 20 (3) ◽  
Author(s):  
Hersanti Hersanti ◽  
Rian Triyanti Rupendi ◽  
Andang Purnama ◽  
Hanudin Hanudin ◽  
Budi Marwoto ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Pseudomonas Fluorescens ◽  
Trichoderma Harzianum ◽  
Ralstonia Solanacearum

Penyakit layu bakteri yang disebabkan oleh R. solanacearum merupakan salah satu penyakit penting yang menyerang tanaman kentang. Salah satu alternatif pengendalian yang ramah lingkungan adalah menggunakan mikroba antagonis seperti Pseudomonas fluorescens, Bacillus subtilis, dan Trichoderma harzianum. Tujuan penelitian adalah  untuk mendapatkan isolat mikroba antagonis terbaik yang paling menekan  penyakit layu bakteri pada tanaman kentang.  Dua percobaan pada penelitian ini menggunakan  Rancangan Acak Lengkap. Percobaan  in vitro menguji daya hambat tiga isolat  P. Fluorescens,  dua isolat B. subtilis, satu isolat B. cereus dan  3 isolat T. harzianum terhadap perkembangan koloni  R. solanacearum pada media King’s B. Percobaan  in vivo menguji kemampuan seluruh isolat terhadap penekanan serangan penyakit layu pada tanaman kentang.  Hasil percobaan menunjukkan bahwa isolat T. harzianum 1, T. harzianum 2 dan T. harzianum 3 mampu menekan penyakit layu bakteri pada tanaman kentang dengan persentase penghambatan berturut-turut 77,21 %,  77,21 %, dan 63,57 %.

Different Responses of Planktonic and Attached Bacillus subtilis and Pseudomonas fluorescens to Sanitizer Treatment

1999 ◽  
Vol 62 (4) ◽  
pp. 368-379 ◽  
Author(s):  
D. LINDSAY ◽  
A. von HOLY
Keyword(s):  
Electron Microscopy ◽  
Hydrogen Peroxide ◽  
Scanning Electron Microscopy ◽  
Bacillus Subtilis ◽  
Pseudomonas Fluorescens ◽  
Chlorhexidine Gluconate ◽  
Plate Counts ◽  
Sessile Cells ◽  
Scanning Electron

Three commercial sanitizers containing iodophor (I), peracetic acid/hydrogen peroxide (PAH), or chlorhexidine gluconate (CG) were evaluated in vitro against planktonic and sessile Bacillus subtilis or Pseudomonas fluorescens cells grown in Standard One Nutrient Broth. Sessile cells were attached to stainless steel or polyurethane test surfaces. Planktonic and attached cells of both bacteria were enumerated by plate counts after sanitizer treatment for 1, 3, or 5 min. Sessile cells were dislodged from test surfaces by shaking them with beads. Cell morphologies were monitored by scanning electron microscopy (SEM). Attached B. subtilis and P. fluorescens cells on both surface types were less susceptible to all three sanitizers than their planktonic counterparts. PAH, I, and CG were equally effective against planktonic P. fluorescens cells, which were reduced by 99.999% after 1, 3, and 5 min exposure. PAH was the only sanitizer effective against attached P. fluorescens cells on both surface types; it reduced counts by ≤99.9% after 1, 3, and 5 min exposure. PAH was also the most effective sanitizer against planktonic B. subtilis cells, reducing counts by 99.9% after 1, 3, and 5 min. Sessile B. subtilis cells on both surface types were the least susceptible to all sanitizers; counts were reduced by only 99.5% or less after exposure to PAH for 5 min. SEM revealed that planktonic and attached cells of both bacteria exhibited symptoms of surface roughness, indentations, and shape distortions after treatment with any of the sanitizers.

PERBANDINGAN DAYA HAMBAT BAKTERI Pseudomonas fluorescens ASAL TOMAT, KEDELAI, DAN JAGUNG TERHADAP Ralstonia solanacearum SECARA IN VITRO

Agrotek ◽  
2018 ◽  
Vol 5 (6) ◽  
Author(s):  
Aco Roni Kirihio ◽  
Ivonne Fitria Mariay ◽  
Cipta Meliala
Keyword(s):  
Pseudomonas Fluorescens ◽  
Filter Paper ◽  
Ralstonia Solanacearum ◽  
Randomized Design ◽  
Completely Randomized Design

<em>Inhibition of Pseudomonas fluorescens isolates the origin of tomato, soybean and corn against Ralstonia solanacearum tested using a completely randomized design (CRD).        P. fluorescens growth was measured at King's B medium by way of suspension antagonist put on filter paper of 0.5 cm in diameter. Inhibition of P. fluorescens is done by placing the antagonist suspension of 0.5 cm diameter filter paper on NA media that has been deployed R. solanacearum. The results showed that: (a) the growth of P. fluorescens origin of tomato, soybean and corn on King's B media were not significantly different, (b) the inhibition of P. fluorescens isolates against R. solanacearum not significantly different and, (c) the inhibition of isolates P. fluorescens origin of tomato, soybean and corn against R. solanacearum in vitro relatively strong</em>

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