scholarly journals Microencapsulation of Gac (Momordica cochinchinensis Spreng.) fruit oil in Ca-Alginate bead produced through Jetcutter

2020 ◽  
Vol 19 (04) ◽  
pp. 80-89
Author(s):  
Vinh Truong
Keyword(s):  
Alginate Bead ◽  
Continuous Operation ◽  
Loading Capacity ◽  
Recovery Efficiency ◽  
Ratio Data ◽  
Alginate Concentration ◽  
Momordica Cochinchinensis ◽  
Cacl2 Concentration ◽  
Fruit Oil ◽  
Ca Alginate

The Gac (Momordica cochinchinensis Spreng.) fruit oil was microencapsulated in Ca-Alginate bead by Jetcutter equipment. Experiments showed that the effects of emulsion preparation and equipment parameters were significant on particle size, microencapsulation yield, loading capacity and solids recovery efficiency of the device. At alginate concentration of 2.5%, the bead had a spherical size of 1.07 - 1.18 mm when the fluid flow was 14 mL/min and the rotation of the cutting disc was 400 - 800 rpm. Samples stored for 2 h after homogenization showed better microencapsulation yield (83 - 84%) and bead size (1.07 mm) than those stored for 24 h after homogenization (79% and 1.18 mm). When the loading capacity increased from 20% to 40%, the mole ratio (CaCl2-alginate) in the bead was reduced from 1.55 to 0.86. This mole ratio data can be considered to control and adjust CaCl2 concentration during the continuous operation of the Jetcutter.

scholarly journals The Influence of Alginate and CaCl2 concentration Towards Betacyanin content and Antioxidant Activity of Red Dragon Fruit (Hylocereus Polyrhizus) restructuring product

2017 ◽  
Vol 2 (2) ◽  
pp. 158
Author(s):  
Wira Wanti Bangalino ◽  
Lydia Ninan Lestario ◽  
Cucun Alep Riyanto
Keyword(s):  
Antioxidant Activity ◽  
Block Design ◽  
Dry Weight ◽  
Weight Basis ◽  
Time Analysis ◽  
Dragon Fruit ◽  
Alginate Concentration ◽  
Wet Weight ◽  
Cacl2 Concentration ◽  
Hylocereus Polyrhizus

<p style="text-align: justify;">The purpose of this study was to determine the effect of alginate and CaCl2 concentration of antioxidant activity and betacyanin content of restructuring product of red dragon fruit, and to determine the best formulation of the product restructuring that produces antioxidant activity and the content of the highest betacyanin on product restructuring. Data were analyzed by 4x2 Factorial Design and Randomized Completely Block Design (RCBD) with 4 replications. As the first factor is the alginate concentration which are 2 %, 3 %, 4 % and 5 %. The second factors are calcium cloride (CaCl2 ) 0.5 % and 0.75 %, while as the block is the time analysis. To test the differences between treatments means, the Honestly Significant of Differences (HSD) were used at 5% level of significant.The results showed that the higher concentrations of alginate and CaCl2 causes declining content of antioxidant activity and betacyanin, the best results are obtained is at a concentration of 2% alginate and CaCl2 0.5% with betacyanin levels 0.537 ± 0.062 mg/g dry weight basis and 0.132 ± 0.011 mg / g wet weight basis. The antioxidant activity of H. polyrhyzus restructuring amounted to 77.452% ± 0.624. ©2016 JNSMR UIN Walisongo. All rights reserved.</p>

scholarly journals Performance Improvement of Ca-Alginate Bead Cross- Linked Laccase from Trametes versicolor IBL-04

BioResources ◽  
2015 ◽  
Vol 11 (1) ◽  
Author(s):  
Sadia Noreen ◽  
Muhammad Asgher ◽  
Fatma Hussain ◽  
Ahsan Iqbal
Keyword(s):  
Performance Improvement ◽  
Trametes Versicolor ◽  
Alginate Bead ◽  
Ca Alginate

Hydrogen sulfide removal utilizing immobilized Thiobacillus sp. IW with Ca-alginate bead

2002 ◽  
Vol 11 (2-3) ◽  
pp. 167-173 ◽  
Author(s):  
Don-Hee Park ◽  
Jin-Myeong Cha ◽  
Hwa-Won Ryu ◽  
Gwang-Woo Lee ◽  
Eui-Youn Yu ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Alginate Bead ◽  
Sulfide Removal ◽  
Hydrogen Sulfide Removal ◽  
Ca Alginate

Use of Ca–alginate as a novel support for TiO2 immobilization in methylene blue decolorisation

2009 ◽  
Vol 60 (4) ◽  
pp. 1081-1087 ◽  
Author(s):  
Juliana Q. Albarelli ◽  
Diego T. Santos ◽  
Sharon Murphy ◽  
Michael Oelgemöller
Keyword(s):  
Methylene Blue ◽  
Alginate Bead ◽  
Industrial Process ◽  
Degradation Efficiency ◽  
Great Promise ◽  
Destructive Effect ◽  
Gel Beads ◽  
Tio2 Gel ◽  
Ca Alginate ◽  
Tio2 Immobilization

This study provides a preliminary contribution to the development of an industrial process for the UV/TiO2 water treatment by introducing a novel support for TiO2 immobilization. For the following study, Methylene Blue (MB) was chosen as the model dye to evaluate this novel immobilization system. The results showed that TiO2 immobilized in a Ca–alginate bead retained its photoactivity during all of the experiments and the TiO2-gel beads presented good stability in water for maintaining its shape after several uses. When a proportion of 10% (v/v) of these beads was used, the configuration system demonstrated an improved mass transfer and consequently enhanced degradation efficiency. Experiments were also performed using ‘recycled’ beads. The results showed an increase in the degradation efficiency when the beads were reused, with an eventual ‘self-destructive’ effect. These studies showed great promise regarding the recyclable reagents with a reduction in waste at no greater cost or reduction in efficiency. Therefore, the potential of TiO2-gel beads as a simple and environmentally friendly catalyst for continuous use was developed.

scholarly journals Influence of Sodium Alginate Concentration on Microcapsules Properties Foreseeing the Protection and Controlled Release of Bioactive Substances

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Kamal Essifi ◽  
Mohamed Brahmi ◽  
Doha Berraaouan ◽  
Abderrahim Ed-Daoui ◽  
Ali El Bachiri ◽  
...  
Keyword(s):  
Uv Spectroscopy ◽  
Release Kinetics ◽  
Polymer Concentration ◽  
Physicochemical Characteristics ◽  
Release Mechanism ◽  
X Ray Diffraction ◽  
Bioactive Substances ◽  
Alginate Concentration ◽  
Bioactive Substance ◽  
Ca Alginate

To understand the abilities of Ca-alginate microcapsules and their specific applications in different fields, it is necessary to determine the physicochemical and structural properties of those formulated microcapsules. In this work, we aimed to study the effect of alginate concentration in the improvement of the encapsulation efficiency (EE) and on the release of phenolic and flavonoid substances. The relationship between the structure of the encapsulated bioactive substance and Ca-alginate network and their effect on the EE and release kinetics have been investigated. The incorporation, structure, morphology, and phase properties of all elaborated materials were characterized by UV-spectroscopy, Fourier transform infrared (ATR-FTIR), scanning electron microscope (SEM), and X-ray diffraction (DRX). The results indicate that increasing the polymer concentration increases the EE and decreases the loading capacity (LC), whereas the effect of alginate polymer concentration on the release was not observed. The release study of bioactive substances showed that the release kinetics is relatively dependent on the structure and the physicochemical characteristics of the bioactive substance, which became clear when the encapsulated compounds were released from the core of calcium alginate microcapsules. Thus, it could be concluded that the pores size of the Ca-alginate network is smaller than the volume of the crocin molecule (2794.926 Å3) and higher than the volume of the gallic acid molecule (527.659 Å3). For the same microcapsules system, the release mechanism is affected by the structure and physicochemical properties of the encapsulated molecules.

scholarly journals A Method of Preserving and Testing the Acceptability of Gac Fruit Oil, A Good Source of β-Carotene and Essential Fatty Acids

2003 ◽  
Vol 24 (2) ◽  
pp. 224-230 ◽  
Author(s):  
L. T. Vuong ◽  
J. C. King
Keyword(s):  
Fatty Acids ◽  
Essential Fatty Acids ◽  
Nutritional Composition ◽  
Daily Consumption ◽  
Momordica Cochinchinensis ◽  
Total Fat ◽  
High Concentrations ◽  
Village Women ◽  
Fruit Oil ◽  
Β Carotene

Gac fruit (Momordica cochinchinensis Spreng) is indigenous to Vietnam and other countries in Southeast Asia. Its seed pulp contains high concentrations of carotenoids, especially the provitamin A, β-carotene. In northern Vietnam, gac fruits are seasonal and are mainly used in making a rice dish called xoi gac. The purpose of this study was to develop a method to collect and preserve gac fruit oil, to evaluate the nutritional composition of the oil, and to assess the acceptability of the gac oil by typical Vietnamese homemakers. One hundred women participated in training to learn how to prepare the fruits and operate the oil press. The women also participated in a survey of gac fruit use and their habitual use of animal fat and vegetable oil. Among all the participants in the training and surveys, 35 women actually produced oil from gac fruits grown in the village, using manual oil presses and locally available materials. The total carotene concentration in gac fruit oil was 5,700 μg/ml. The concentration of β-carotene was 2,710 μg/ml. Sixty-nine percent of total fat was unsaturated, and 35% of that was polyunsaturated. The average daily consumption of gac fruit oil was estimated at 2 ml per person. The daily β-carotene intake (from gac fruit oil) averaged approximately 5 mg per person. It was found that gac oil can be produced locally by village women using manual presses and locally available materials. The oil is a rich source of β-carotene, vitamin E, and essential fatty acids. Although the β-carotene concentration declines with time without a preservative or proper storage, it was still high after three months. The oil was readily accepted by the women and their children, and consumption of the oil increased the intake of β-carotene and reduced the intake of lard.

scholarly journals Influence of Chitosan-Alginate Microcapsules Containing Anti-Vibrio Harveyi IgY in the Gastrointestinal Tract Simulation

2015 ◽  
Vol 9 (12) ◽  
pp. 110 ◽  
Author(s):  
Kawin Punyokun ◽  
Ratchanee Hongprayoon ◽  
Prapansak Srisapoome ◽  
Theerapol Sirinarumitre
Keyword(s):  
Egg Yolk ◽  
Gastric Fluid ◽  
Simulated Gastric Fluid ◽  
Gastrointestinal Infections ◽  
Simulated Intestinal Fluid ◽  
Egg Yolk Immunoglobulin ◽  
Alginate Concentration ◽  
Cacl2 Concentration ◽  
The Stability ◽  
Processing Factors

<p class="zhengwen"><span lang="EN-GB">Egg yolk immunoglobulin (IgY) is an alternative treatment for the prevention of gastrointestinal infections in pigs, cows, chickens and fish. In our previous report, we preliminarily proved that anti-<em>V. harveyi</em> IgY had effective potential to control luminous disease in black tiger shrimp. However,</span><span lang="EN-GB">IgY activity may be reduced or destroyed by gastric conditions, particularly low pH and digestive enzymes. Therefore, it is necessary to find an effective method to preserve the therapeutic function of IgY antibodies during gastric passage. Chitosan-alginate microcapsules have been developed to protect IgY from gastric inactivation. The processing factors included different forms of chitosan and alginate, while a CaCl2 concentration and encapsulation medium was investigated. The optimum results were obtained under the following conditions: High Mw chitosan concentration 0.2% (w/v), medium viscosity alginate concentration 2% (w/v), CaCl2 concentration 0.5% (w/v). The stability of IgY in simulated gastric fluid (SGF, pH 1.2) was greatly improved by encapsulation in chitosan-alginate microcapsules, and retained greater than 90% activity after 2 h exposure to SGF. Less than 10% IgY was released upon the microcapsules’ exposure to SGF for 2 h, and more than 80% IgY was released upon the microcapsules’ exposure to simulated intestinal fluid (SIF, pH 6.8) for 16 h. </span></p>

scholarly journals Immobilization and Characterization of Bacillus Thuringiensis HCB6 Amylase in Calcium Alginate Matrix

Molekul ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. 70 ◽  
Author(s):  
Zusfahair Zusfahair ◽  
Dian Riana Ningsih Dian Riana Ningsih ◽  
Dwi Kartika Dwi Kartika ◽  
Amin Fatoni Amin Fatoni ◽  
Indah Permatawati Indah Permatawati
Keyword(s):  
Incubation Time ◽  
Substrate Concentration ◽  
Contact Time ◽  
Alginate Bead ◽  
Residual Activity ◽  
Immobilized Enzymes ◽  
Optimum Ph ◽  
Km Value ◽  
Ca Alginate

Free enzyme in solution react with substrates to result in products which cannot be recovered for reuse. These problems can be overcome to a certain extent by the use of enzyme immobilization method. Immobilized enzymes are more robust and more resistant to condition changes. More importantly, the heterogeneous immobilized enzyme systems allow an easy recovery of both enzymes and products, multiple re-uses of enzymes, and continuous operation of enzymatic processes. Entrapment of enzymes in Ca-alginate is one of the simplest methods of immobilization. The aim of this research was to obtain the optimum condition of the making of immobilized amylase beads using a Ca-alginate bead and to determine its characteristics. The optimization of immobilized amylase beads includes variation of sodium alginates and variations of enzyme contact time with CaCl2. The characterization of immobilized amylase includes determination of optimum substrate concentration, optimum pH, and optimum incubation time as well as amylase stability test. Amylase activity was determined by using dinitro salicylic (DNS) method. The results showed that the optimum immobilized amylase obtained at alginate concentrations of 5% (w/v), contact time of 60 minutes and immobilization efficiency of 67.5%. Furthermore, immobilized amylase showed optimum substrate concentration of 1.5-2.5% (w/v), optimum pH of 6, an optimum incubation time of 20 minutes with the activity of 179.8 U/mL. The KM value for free amylase and immobilized amylases were 0.3 mM and 0.12 mM respectively. Vmax value for free amylase and immobilized amylases were 105.3 U/mL and 10.1 U/mL respectively. Immobilized Amylase can be used up to six times with the residual activity of 52.7%.

scholarly journals Release kinetics of lime essential oil (Citrus aurantifolia) from beads microencapsulated through ion-gel method

2021 ◽  
Vol 20 (1) ◽  
pp. 58-66
Author(s):  
Vinh Truong
Keyword(s):  
Essential Oil ◽  
Release Rate ◽  
Release Kinetics ◽  
Chitosan Beads ◽  
Gel Method ◽  
Citrus Aurantifolia ◽  
Alginate Concentration ◽  
Significant Difference ◽  
Ion Gel ◽  
Ca Alginate

Ca-Alginate beads for microencapsulation of lime oil (Citrus aurantifolia) by ion-gel method was manufactured and then soaked in Chitosan solution to obtain Ca-Alginate-Chitosan beads. Increased CaCl2 concentrations reduced lime essential oil release. The alginate concentration (2 to 3%) and water temperature significantly affected the release of oil (P < 0.05). The higher temperatures, the higher the release rate. The oil release at 75oC was twice as much as at 45oC. At 45oC, the difference in the release rate among the alginate concentrations of 2%, 2.5% and 3% was clear and statistically significant (P < 0.05). However, at 60oC and 75oC, there was no significant difference in release between the alginate concentration of 2.5% and 3% (P > 0.05). In the storage of beads in 1% CaCl2 solution at normal temperature, after the first 15 days, Ca Alginate-Chitosan system released about 3% slower than Ca-Alginate system, but there was no difference between the two systems after 45 days. This shows that if prolonged for a long time, the ion-gelation of Ca-Alginate prevails over the Alginate-Chitosan cross-link.

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