MICROBIOLOGICAL SCREENING AND DETERMINATION OF ANTIBIOTIC RESISTANCE OF THE UROPATHOGEN ESCHERICHIA COLI IN PREGNANT WOMEN IN THE CLINIC OF THE ALMATY СITY HOSPITAL №7

Vestnik ◽  
2021 ◽  
pp. 75-80
Author(s):  
В.Н. Сон ◽  
М.Р. Рысулы ◽  
С.Т. Турсыналиев ◽  
М.Е. Рамазанов
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Pregnant Women ◽  
Asymptomatic Bacteriuria ◽  
E Coli ◽  
Screening Programs ◽  
Susceptibility Study

Бессимптомная бактериурия определяется как присутствие бактерий в моче беременных без признаков или симптомов инфекции мочевыводящих путей (ИМП). Стандартное микробиологическое исследование образцов мочи проведено с июня 2019 года по декабрь 2020 года у 800 беременных по программам скрининга на наличие мочеполовой инфекции (ИМП) в поликлинике городской клинической больницы №7 г.Алматы. Образцы были протестированы микробиологически по стандартным методикам, рекомендованных CAESAR. Выбор антимикробных препаратов (АМП) для включения в исследование чувствительности, а также принципы интерпретации результатов проводились согласно рекомендации Европейского комитета по определению чувствительности к антибиотикам (EUCAST). Представлены результаты оценки резистенности и чувствительности к антимикробным препаратам 138 клинических изолятов Escherichia coli, выделенных из образцов мочи беременных пациенток поликлиники №35 ГКБ №7 УОЗ г.Алматы. Основными уропатогеном в 66,3% являются E. Coli, меньше - в 15,9% Staphylococcus aureus и в 8,2% Klebsiella pneumoniae. Чувствительность изолятов Escherihia coli была для левофлоксацина (76,6%), комбинации пиперациллин/тазобактаму (73,7%), ципрофлоксацину (71,5%), тетрациклину (74,4%), но низкой для пиперациллина (24.8%), цефураксима (38,6%), тобрамицина (40,9%), цефазилина (40,1%), цефепима (40,1%); цефтазидима (43%), комбинации триметоприм/сульфаметаксазол (43,8%). Чувствительность E. coli к карбапенемам - для имипенема (73%), меропенема (67,8%), эртапенема (76,6%), а устойчивость составляла для эртапенема (13,8%), меропенема (16%) и имипенема (3,6%). Asymptomatic bacteriuria is defined as the presence of bacteria in the urine of pregnant women without signs or symptoms of a urinary tract infection (UTI). A standard microbiological study of urine samples was carried out from June 2019 to December 2020 in 800 pregnant women under screening programs for the presence of urinary tract infection (UTI) in the ambulatory of the Almaty city clinical hospital № 7. The samples were tested microbiologically according to standard methods recommended by CAESAR. The choice of AMPs for inclusion in the susceptibility study, as well as the principles of interpretation of the results, were carried out according to the recommendation of the European Committee for the determination of antibiotic susceptibility (EUCAST). The sensitivity of Escherihia coli isolates was for levofloxacin (76.6%), piperacillin / tazobactam combination (73.7%), ciprofloxacin (71.5%), tetracycline (74.4%), but low for piperacillin (24.8%), cefuraxime (38.6%), tobramycin (40.9%), cefazilin (40.1%), cefepime (40.1%); ceftazidime (43%), trimethoprim / sulfametaxazole combination (43.8%). The sensitivity of Escherihia coli to carbapenems was for imipenem (73%), meropenem (67.8%), ertapenem (76.6%), and resistance was for ertapenem (13.8%), meropenem (16%) and imipenem (3,6%).

scholarly journals Role of Type 1 Fimbria- and P Fimbria-Specific Adherence in Colonization of the Neurogenic Human Bladder by Escherichia coli

2002 ◽  
Vol 70 (11) ◽  
pp. 6481-6484 ◽  
Author(s):  
Richard A. Hull ◽  
William H. Donovan ◽  
Michael Del Terzo ◽  
Colleen Stewart ◽  
Margaret Rogers ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Asymptomatic Bacteriuria ◽  
Model Systems ◽  
Human Beings ◽  
Human Bladder ◽  
E Coli

ABSTRACT Recent clinical studies suggest that the deliberate colonization of the human bladder with a prototypic asymptomatic bacteriuria-associated bacterium, Escherichia coli 83972, may reduce the frequency of urinary tract infection in individuals with spinal cord injuries. However, the mechanism by which E. coli 83972 colonizes the bladder is unknown. We examined the role in bladder colonization of the E. coli 83972 genes papG and fimH, which respectively encode P and type 1 receptor-specific fimbrial adhesins. E. coli 83972 and isogenic papGΔ and papGΔ fimHΔ mutants of E. coli 83972 were compared for their capacities to colonize the neurogenic human bladder. Both strains were capable of stable colonization of the bladder. The results indicated that type 1 class-specific adherence and P class-specific adherence, while implicated as significant colonization factors in experiments that employed various animal model systems, were not required for colonization of the neurogenic bladder in human beings. The implications of these results with regard to the selection of potential vaccine antigens for the prevention of urinary tract infection are discussed.

scholarly journals Asymptomatic Bacteriuria among Pregnant Women Attending a Tertiary Care Hospital in Western Nepal: A Cross-sectional Prospective Study

2021 ◽  
Vol 9 (01) ◽  
pp. 38-42
Author(s):  
Shristi Raut ◽  
Sulochana Khatiwada ◽  
Narayan GC
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Pregnant Women ◽  
Asymptomatic Bacteriuria ◽  
Urine Samples ◽  
Diffusion Method ◽  
Care Hospital ◽  
Significant Bacteriuria

INTRODUCTION Urinary tract of female undergoes tremendous changes during pregnancy which increases their risk of acquiring infection. Asymptomatic bacteriuria is a common entity among pregnant women which refers to significant bacteriuria (>105 bacteria per ml of urine) without any typical symptoms of urinary tract infection. Asymptomatic bacteriuria can lead to various maternal and fetal complications if not detected and treated on time. MATERIAL AND METHODS Total 280 urine samples were collected in sterile universal containers from pregnant women not showing typical symptoms of urinary tract infection at the time of sample collection. Urine samples were inoculated in both MacConkey agar and Blood agar by semi quantitative culture method. Culture plates were reported after 24 hours of incubation at 370C. Bacteria isolated were subjected to antibacterial susceptibility testing by modified Kirby-Bauer disc diffusion method. RESULTS Out of 280 urine cultures, 213 samples were sterile. Significant bacteriuria was seen in 25 cases (8.9%) followed by insignificant bacteriuria (20, 7.14%) and contamination (17, 6.10%). Highest number of cultures positive were in age group 21-30 years (19, 9.1%,). Out of 25 cases of significant bacteriuria, 60% were primigravida and 40% were multigravida. The most common organism isolated was Escherichia coli (10, 60%) followed by Klebsiella pneumoniae (5, 40%). CONCLUSION Screening of all pregnant women for asymptomatic bacteriuria is essential during their antenatal checkup. Escherichia coli is the commonest organism that cause asymptomatic bacteriuria. Appropriate antibiotic therapy is absolutely necessary for positive cases on the basis of antimicrobial susceptibility result to prevent unwanted obstetric complications.

scholarly journals Virulence factors in Escherichia coli urinary tract infection.

1991 ◽  
Vol 4 (1) ◽  
pp. 80-128 ◽  
Author(s):  
J R Johnson
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Virulence Factors ◽  
Virulence Factor ◽  
Asymptomatic Bacteriuria ◽  
Clinical Role ◽  
E Coli ◽  
Serum Killing

Uropathogenic strains of Escherichia coli are characterized by the expression of distinctive bacterial properties, products, or structures referred to as virulence factors because they help the organism overcome host defenses and colonize or invade the urinary tract. Virulence factors of recognized importance in the pathogenesis of urinary tract infection (UTI) include adhesins (P fimbriae, certain other mannose-resistant adhesins, and type 1 fimbriae), the aerobactin system, hemolysin, K capsule, and resistance to serum killing. This review summarizes the virtual explosion of information regarding the epidemiology, biochemistry, mechanisms of action, and genetic basis of these urovirulence factors that has occurred in the past decade and identifies areas in need of further study. Virulence factor expression is more common among certain genetically related groups of E. coli which constitute virulent clones within the larger E. coli population. In general, the more virulence factors a strain expresses, the more severe an infection it is able to cause. Certain virulence factors specifically favor the development of pyelonephritis, others favor cystitis, and others favor asymptomatic bacteriuria. The currently defined virulence factors clearly contribute to the virulence of wild-type strains but are usually insufficient in themselves to transform an avirulent organism into a pathogen, demonstrating that other as-yet-undefined virulence properties await discovery. Virulence factor testing is a useful epidemiological and research tool but as yet has no defined clinical role. Immunological and biochemical anti-virulence factor interventions are effective in animal models of UTI and hold promise for the prevention of UTI in humans.

scholarly journals degS Is Necessary for Virulence and Is among Extraintestinal Escherichia coli Genes Induced in Murine Peritonitis

2003 ◽  
Vol 71 (6) ◽  
pp. 3088-3096 ◽  
Author(s):  
Peter Redford ◽  
Paula L. Roesch ◽  
Rodney A. Welch
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Luria Bertani ◽  
Broth Culture ◽  
Wild Type ◽  
E Coli ◽  
Virulence Attenuation

ABSTRACT Extraintestinal Escherichia coli strains cause meningitis, sepsis, urinary tract infection, and other infections outside the bowel. We examined here extraintestinal E. coli strain CFT073 by differential fluorescence induction. Pools of CFT073 clones carrying a CFT073 genomic fragment library in a promoterless gfp vector were inoculated intraperitoneally into mice; bacteria were recovered by lavage 6 h later and then subjected to fluorescence-activated cell sorting. Eleven promoters were found to be active in the mouse but not in Luria-Bertani (LB) broth culture. Three are linked to genes for enterobactin, aerobactin, and yersiniabactin. Three others are linked to the metabolic genes metA, gltB, and sucA, and another was linked to iha, a possible adhesin. Three lie before open reading frames of unknown function. One promoter is associated with degS, an inner membrane protease. Mutants of the in vivo-induced loci were tested in competition with the wild type in mouse peritonitis. Of the mutants tested, only CFT073 degS was found to be attenuated in peritoneal and in urinary tract infection, with virulence restored by complementation. CFT073 degS shows growth similar to that of the wild type at 37°C but is impaired at 43°C or in 3% ethanol LB broth at 37°C. Compared to the wild type, the mutant shows similar serum survival, motility, hemolysis, erythrocyte agglutination, and tolerance to oxidative stress. It also has the same lipopolysaccharide appearance on a silver-stained gel. The basis for the virulence attenuation is unclear, but because DegS is needed for σE activity, our findings implicate σE and its regulon in E. coli extraintestinal pathogenesis.

scholarly journals CAPSULE PRODUCTION PATTERN OF UROPATHOGENIC ESCHERICHIA COLI OF URINARY TRACT INFECTION PATIENTS IN KIRKUK HOSPITALS

2020 ◽  
Vol 17 (35) ◽  
pp. 621-627
Author(s):  
May Ali Hussien UMRAN ◽  
Sumaya Najim AL-KHATEEB
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Clinical Manifestations ◽  
Uropathogenic Escherichia Coli ◽  
E Coli ◽  
Intestinal Pathogens ◽  
Production Pattern ◽  
Living Organisms

The bacterium Escherichia coli is one of the best free-living organisms studied in depth. It is a surprisingly diverse species, since some strains of E. coli live in the intestine of animals as harmless commensals, while other distinct genotypes, such as an enteropathogenic or enterohemorrhagic E. coli, for example, cause morbidity and death marked as human intestinal pathogens. The purpose of this study was to develop and validate a PCR assay for a known and suspected uropathogenic E. coli virulence factor (kpsMT) gene region to determine the distribution of the gene and its role in the development of clinical diseases of the urinary system. A total of 25 urine samples were collected from patients with urinary tract infection (UTI) at Azadi and Kirkuk hospitals in the city of Kirkuk, Iraq. Samples of both genders and different ages were collected from patients with suspected urinary tract infection according to the clinical manifestations and symptoms diagnosed by the examining physician. The samples were cultured and positive samples were subjected to the IMViC test to identify E. coli bacteria and subsequently identified using the Vitek 2 compact system. Among 25 samples, 24 (96%) showed positive results for bacterial cultural growth. Of these, 17 (68%) were identified as Escherichia coli. Of the total of 17 isolates, 14 from patients with mild urinary tract infection, and 3 from patients with Urosepsis. The kpsMT gene was present in 14 isolates (82.3%), including 11 (78.5%) isolates from patients with mild urinary tract infection, and 3 (100%) isolates from patients with Urosepsis. It was concluded that Escherichia coli is the most prevalent in urine tract infection samples. Due to the abundance of the kpsMT gene in uropathogenic Escherichia coli (UPEC), this gene plays an important role in developing UTI if it is not treated correctly and quickly; mild cases of UTI can turn into Urosepsis.

scholarly journals Evaluation of ESBL (Extended Spectrum Beta-Lactamase) Producing Escherichia Coli Isolated from Patients with Urinary Tract Infection by Phenotypic and Genotypic Methods

2020 ◽  
Author(s):  
Mohammad Hasan Namaei ◽  
Hengameh Hamzei ◽  
Marzie Moghanni ◽  
Azadeh Ebrahimzadeh
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Antibiotic Use ◽  
M Gene ◽  
Esbl Producer ◽  
Accurate Identification ◽  
E Coli ◽  
Tract Infections

Abstract Background: Urinary Tract Infection (UTI) is the most common bacterial infection in the world. E. coli is the predominant Pathogen. This study evaluates the prevalence of ESBL in E. colis isolated from patients with urinary tract infections with phenotypic and genotypic methods.Methods: This descriptive-analytical study was done on 155 isolates of E. coli isolated from patients with urinary tract infection who had received the study consent. After accurate identification of E. coli strains. ESBL production for Escherichia coli isolates which are resistant to ceftriaxone or ceftazidime was evaluated by CDT method. TEM, SHV and CTX-M genes were identified by PCR.Results: The results showed that 30 strains from 155 strains of E. coli had ESBL. Strains of ESBL producer were more in males was lower in educated persons. 38.9% of ESBL producer had antibiotic use, 29.9% -producing Escherichia hospitalization and 31.6% uti history. The highest level of drug allergy in the ESBL was related to nitrofurantoin, and the highest resistance was related to cefazolin, co-trimoxazole. The CTX-M and the CTX-M15 gene were found in 92.7% and 57.1% of cases, respectively; also the SHV and TEM genes were not found in any of ESBL-producing Escherichia coli strains. Most therapeutic response in patients was related to cefexime, ciprofloxacin and nitrofurantoin 27.4%, 26% 21.9%, respectively.Conclusion: This study showed that the history of antibiotic use, hospitalization, uti related to increase of ESBL-producing in E. coli isolates., the CTMX-M gene is the most common gene in ESBL-producing E. coli strains.

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