scholarly journals Documented Lipid Per oxidative Intoxication of Cerebellar Molecular Cell Layer

2021 ◽  
Vol 15 (7) ◽  
pp. 1573-1574
Author(s):  
Zaheer Amjad ◽  
Tazeen Kohari ◽  
Zaffar Malick
Keyword(s):  
Nervous Tissue ◽  
Cell Layer ◽  
Molecular Layer ◽  
Lithium Carbonate ◽  
Granule Cell Layer ◽  
Rat Cerebellum ◽  
Group A ◽  
Ad Libitum ◽  
Group B ◽  
Cerebellar Molecular Layer

Background: Cerebellar cortexconsists of three layers. The outer molecular, middlePurkinje cell layer, inner granule cell layer. Lithium the alkali metal hasdeleterious effects on nervous tissue and this study proved the injurious effects of lithium on molecular cell layer of cerebellum. Aim: To observe and report the damaging histological and morphological change of the decrement in the thickness of cerebellar molecular layer by Lithium. Methods: This study was designed to observe the microscopic changes of thickness of molecular layer in rat cerebellum. For this experimental study 12 animals were used, they were divided into two groups, each comprising of 6 animals. Results: Group-A received normal lab diet and water ad libitum while group B received injectable lithium carbonate 20 mg/kg/ for 4 weeksrespectively. Micrometry was done and changes of the thickness of molecular cell layer were recorded and documented. Conclusion: The pernicious effects of Lithium Carbonate on molecular cerebellar cortex were visualized and evaluated .Highly significantly decreased changes of thickness of molecular cell layer were documented in rat cerebellum. Keywords: Lipidperoxidation,Molecular cell layeratrophy, decomposition

To Evaluate the Toxic Effects of Lithium Carbonate on Granule Cells Count of Rat Cerebellum

2021 ◽  
Vol 15 (6) ◽  
pp. 1149-1151
Author(s):  
T. Kohari ◽  
Z. Malik ◽  
A. Ahmed ◽  
F. Irshad ◽  
A. Rasheed ◽  
...  
Keyword(s):  
Granule Cells ◽  
Lithium Carbonate ◽  
Granule Neurons ◽  
Oxidant Injury ◽  
Rat Cerebellum ◽  
Documented Information ◽  
Group A ◽  
Chronic Ingestion ◽  
Group B ◽  
Therapeutic Doses

Aim: To study the damaging effect of chronic ingestion of 20 mg/kg body weight/OD of lithium carbonate on cerebellargranule cells. Methods: However, there is scanty documented information about the cerebellar toxicities of lithium carbonate on granule neurons. Therefore the present study is designed to observe the microscopic changes of granule neurons in rat cerebellum. For this experimental study 20 animals were used, they were divided into two groups, each comprising of 10 animals. Results: Group-A received normal lab diet and water ad libitum while group B received lithium carbonate 20 mg/kg/OD for 2 weeks and 6 weeks respectively. Micrometry was done on granule cells count. Conclusion: Highly significant changes of granule cells count were observed even at therapeutic doses. Lithium carbonate causes oxidant injury to granule neuronal cells in rat cerebellum. Keywords: Oxidant injury, Cerebellar degeneration, Incoordination,

Decrement of the Purkinje Cells Diameter after Oral Intake of Lithium in Albino Rats

2021 ◽  
Vol 15 (8) ◽  
pp. 1788-1789
Author(s):  
Tazeen Kohari ◽  
Farah Malik ◽  
Aftab Ahmad
Keyword(s):  
Purkinje Cell ◽  
Purkinje Cells ◽  
Gray Matter ◽  
Oral Intake ◽  
Lithium Carbonate ◽  
Albino Rats ◽  
Cell Diameter ◽  
Group A ◽  
Cerebellar Gray Matter ◽  
Group B

Background: The histology of Cerebellar gray matter consists of a middle Purkinje cells layer with flask shaped Purkinje cells. The field of Neurology has documented that different organic compounds and metals are lethal to the excitatory Purkinje Neurons. Researches have proved Lithium to be hazardous to nervous tissue and especially Cerebellum For the past sixty years Lithium is the favorable drug for treatment of Bipolar Disorder. Aim: To Analyse and record the changes of decrement of the size of Purkinje cell Diameter after chronic Lithium ingestion. Methods: Sixteen albino rats were selected and were treated with lithium for a period of fifteen days and the data for changes in Purkinje cells Diameter was observed. Results: The Observations of Our study showed highly significantly decreased diameter of the Purinje cells in Group B (Lithium Carbonate) animals as compared to Group A Animals which were on Lab Diet Conclusion: The Morphometric Data proved that Lithium Carbonate is Toxic to Purkinje cells, and it educated our Population to use Lithium with caution. Keywords: Purkinje cell Diameter, Gray matter, Hazardous

To Study the Deteriorating Effects of Lithium Carbonate on Thyroid Gland - A Study in Albino Rats

2021 ◽  
Vol 15 (12) ◽  
pp. 3292-3293
Author(s):  
Tazeen Kohari ◽  
Zaffar Iqbal Malik ◽  
Aftab Ahmad ◽  
Rana M. Asad Khan
Keyword(s):  
Thyroid Gland ◽  
Harmful Effect ◽  
Lithium Carbonate ◽  
Diet Group ◽  
Human Thyroid ◽  
Control Group ◽  
Albino Rats ◽  
Weight Changes ◽  
Group A ◽  
Group B

Background: The human thyroid gland is located in the front of neck. It consists of two lobes. The two lobes are joined with each other by isthmus. The mood stabilizer Lithium Caronate has deleterious effects on the thyroid gland. Aim: To observe and report the data of the harmful effect of Lithium on the weight changes of thyroid gland. Methods: Sixteen rats were selected for this experimental study. The rodents were divided into two groups. Group A comprised of eight animals which were given laboratory diet, Group B contained eight albinos who were given Tablet Lithium Carbonate in powder form at a dose of 60 mg/day for four weeks. After completion of the study time animals were sacrificed and thyroid gland weight were recorded and compared in both groups. Results: The results in both groups were recorded and compared .It was reported that Group B animals had a highly significantly decreased thyroid weight after four weeks Lithium ingestion than Group A control group. Conclusion: The results of our study concluded that Lithium Carbonate damages thyroid glandular tissue and causes its weight to decline. Key words: Thyroid gland, Isthmus, deteriorating

scholarly journals Omeprazole and misoprostol for preventing gastric mucosa effects caused by indomethacin and celecoxib in rats

2006 ◽  
Vol 21 (3) ◽  
pp. 168-176 ◽  
Author(s):  
Míriam Elias Cavallini ◽  
Nelson Adami Andreollo ◽  
Konradin Metze ◽  
Marina Raquel Araújo
Keyword(s):  
Gastric Mucosa ◽  
Microscopic Analysis ◽  
Control Group ◽  
Average Weight ◽  
Gastric Cytoprotection ◽  
Microscopic Studies ◽  
Group A ◽  
Ad Libitum ◽  
Pre Treatment ◽  
Group B

PURPOSE: To evaluate and to compare macro and microscopically the intense injuries of the gastric mucosa of rats which were caused by NSAIDS celecoxib and indomethacin and the gastric cytoprotection with omeprazole and misoprostol. METHODS: The sample is formed by one hundred and fifty Wistar rats with average weight 200 g, distributed in four groups, such as: Group A, subdivided in groups A1 and A2 - pre-treatment with omeprazole (20 mg/rat) during seven days and on the 8th day - use of NSAIDS, concerning A1 (20 rats) were given celecoxib (1mg/rat) and A2 (20 rats) were given indomethacin. The Group B, subdivided in group B1 and B2 - pre-treatment with misoprostol (20mg/rat) during seven days and on the 8th day use of NSAIDS, concerning B1 (20 rats) were given celecoxib (1 mg/ rat) and B2 (20 rats) were given indomethacin (12.5 mg/rat). The Group C: were not given cytoprotection during seven days, from the 7th to the 8th day - fast of food and water ad libitum, on the 8th day of NSAIDS use, concerning C1 (20 rats) were given celecoxib, C2 (20 rats) were given indomethacin (12.5 mg/ rat), C3 (20 rats) were given celecoxib (200mg/rato), and Group D - control group, concerning 10 rats were observed during seven days ingesting food and water ad libitum. On the 9th day, the stomachs were taken out and were macro and microscopically evaluated for the identification of the gastric injuries. RESULTS: On the macroscopic studies, the groups A2, B2 and C2 presented a remarkable high number of injuries for cm² /animal, respectively 18.55 injuries for cm² /animal, 16.25 injuries for cm² /animal and 13.55 injuries for cm²/animal. On the microscopic studies, the percentage of the injured mucosa, presented expressive difference among the groups A1, B1, C1 when compared to the groups A2, B2, C2 (p<0.0001). The average of the length/injury and the average of the depth of the injuries did not present expressive statistics differences among the groups A2, B2 and C2. The average of the edema presented expressive statistics difference among the groups A2 and D; B2 and C2 and between C2 and D (p < 0.05). CONCLUSIONS: The indomethacin on the applied concentration causes a great number of macroscopic and microscopic injuries to gastric mucosa of rats when compared to celecoxib which does not cause lesions. Omeprazole and misoprostol on the applied concentrations do not present macroscopic and microscopic effectiveness on the gastric cytoprotection when applying indomethacin. Considering the microscopic analysis of the average of the edema, the group of animals, which was given misoprostol as cytoprotection, presented a lower average compared to the group which was given omeprazole.

Nutrient metabolism and rumen micro-organisms in sheep fed a poor-quality tropical grass hay supplemented with sulphate

1990 ◽  
Vol 115 (2) ◽  
pp. 269-275 ◽  
Author(s):  
M. Morrison ◽  
R. M. Murray ◽  
A. N. Boniface
Keyword(s):  
Feed Intake ◽  
Rumen Fluid ◽  
Poor Quality ◽  
Nutrient Metabolism ◽  
Heteropogon Contortus ◽  
Molar Proportion ◽  
Group A ◽  
Ad Libitum ◽  
The Difference ◽  
Group B

SUMMARYA feeding trial was performed during 1986, in Townsville, Australia, to describe alterations in nutrient metabolism, and the coincident gross changes in rumen microbiology, when a sulphurdeficient diet was supplemented with inorganic sulphur. Eight Merino sheep were fedad libituma spear grass (Heteropogon contortus) hay of low sulphur content (0·4 g/kg DM), supplemented with all other essential minerals. Upon supplementation, daily sulphur intake was increased toc.0·75 g and four animals continued to be fedad libitum(group A) whilst the remaining animals were restricted in feed intake (group B). Sulphur supplementation caused a twofold increase in the feed intake of group A (P< 0·05). In both groups of animals, sulphur supplementation increased the fermentation of cotton thread cellulose (P< 0·05), as well as of ground plant dry matter (P< 0·05), suspended in the rumen in nylon bags for 24 and 48 h. The apparent digestibility of organic matter (AOMD) was also increased by sulphur supplementation; from 30·6 to 39·3% in group A (P< 0·05) and from 35·1 to 41·5% in group B (P> 0·05). The difference in AOMD between groups with sulphur supplementation was not significant, despite group B maintaining a longer retention of fluid digesta in the rumen (20·7 v. 25·3 h) with a significantly lowered rumen volume (4·68 v 3·67 litres,P< 0·05) and outflow of fluid digesta from this site (5·31 v 3·58 litres/day,P< 0·05). Sulphur supplementation increased the molar proportion of acetic acid in rumen fluid (P< 0·05) but lowered propionic and butyric acids in group A (P< 0·05). Only the molar proportion of propionic acid in rumen fluid was significantly lowered in group B (P< 0·05). In both groups of animals, counts of fluid-borne rumen bacteria, protozoa and sporangia of rumen anaerobic fungi (RAF) all increased significantly with sulphur supplementation (P< 0·05 in all instances), but no significant differences were observed between groups in microbial counts, despite the different feeding regimes. The increase in sporangial forms of RAF was most marked; no sporangial forms were detectable until the diet was supplemented with sulphate and measurable concentrations of rumen sulphide were observed. This is the first account of nondetectable concentrations of RAF brought about by the nutritional status of the diet.

Trigeminocerebellar mossy fiber branching to granule cell layer patches in the rat cerebellum

1981 ◽  
Vol 209 (2) ◽  
pp. 255-269 ◽  
Author(s):  
Donald C. Woolston ◽  
Jeffrey Kassel ◽  
John M. Gibson
Keyword(s):  
Granule Cell ◽  
Cell Layer ◽  
Mossy Fiber ◽  
Granule Cell Layer ◽  
Rat Cerebellum

scholarly journals A comparison of liveweight gain of two groups of weaners of different entry liveweight in an ad libitum fodder beet feeding system for finishing beef cattle

2015 ◽  
Vol 77 ◽  
pp. 23-28 ◽  
Author(s):  
S.J. Gibbs ◽  
B. Saldias ◽  
J. White ◽  
D. Walsh ◽  
N. Stocker ◽  
...  
Keyword(s):  
Beef Cattle ◽  
Cost Effective ◽  
High Energy ◽  
Feeding System ◽  
Finishing Cattle ◽  
Fodder Beet ◽  
Group A ◽  
Ad Libitum ◽  
Group B ◽  
Finishing Beef Cattle

The use of fodder beet as the primary diet for finishing beef cattle is a recent New Zealand innovation. A system using ad libitum grazed fodder beet through autumn and winter to finish spring-born steers between 12 and 18 months old was developed. Fodder beet is a high energy yet comparatively low protein content feed, and the fodder beet system requires high liveweight gains on the crop and an early slaughter age for cost effective production. Lower liveweight weaners have a greater protein requirement for optimal weight gain, and the threshold entry liveweight for satisfactory performance in this feeding system was not yet established. The objective of this study was to quantify the effect of the entry liveweight of weaners on liveweight gain in the fodder beet feeding system. A group of 392 rising 1-year-old beef cattle fed using this system were divided at entry into three groups on unfasted liveweight (kg): A, 290-370; B, 240-260; and the remaining animals. The heaviest (group A: steer n=67, 316 ±2.7 kg; heifer n=45, 312 ±2.5 kg) and lightest (group B: steer n=77, 249 ±0.7 kg; heifer n=19, 251 ±1.0 kg) groups were then treated identically on ad libitum fodder beet to compare liveweight gain across 130 days on crop. The mean liveweight daily gain (kg/ day) for group A was significantly greater than group B, and greater for the steers compared to heifers in group A (0.98 and 0.85), but not in group B (0.82 and 0.81). Mean liveweight gain as a proportion of entry liveweight was greater in group B, and greater in steers. Both mean daily liveweight gain and mean liveweight gain as a proportion of entry liveweight were positively correlated with entry liveweight, but the R2 of both was low (0.14 and 0.05, respectively). Mean daily liveweight gain values of this magnitude are greater than those previously reported for alternative winter crops, and satisfactory for finishing beef cattle in this system. This study suggests the use of beef cattle above 240 kg liveweight as weaners in this fodder beet feeding system, both steers and heifers, is satisfactory for achieving acceptable slaughter liveweight before 18 months old. Keywords: beef production, fodder beet, finishing cattle, ad libitum fodder beet intakes.

Altered synaptic transmission in dentate gyrus of rats reared in complex environments: evidence from hippocampal slices maintained in vitro

1986 ◽  
Vol 55 (4) ◽  
pp. 739-750 ◽  
Author(s):  
E. J. Green ◽  
W. T. Greenough
Keyword(s):  
Synaptic Transmission ◽  
Dentate Gyrus ◽  
Granule Cell ◽  
Granule Cells ◽  
Cell Layer ◽  
Molecular Layer ◽  
Hippocampal Slices ◽  
Granule Cell Layer ◽  
Complex Environments ◽  
Rearing Environment

Pre- and postsynaptic responses to activation of medial perforant path (MPP) axons were examined in hippocampal slices taken from rats reared for 3-4 wk in relatively complex (EC) or individual cage (IC) environments. Three types of extracellular field potentials were recorded in the infrapyramidal blade of the dentate gyrus: 1) granule cell population spikes (PSs), which reflect the number and synchrony of discharging granule cells (2), 2) population excitatory postsynaptic potentials (EPSPs), which reflect the amount of excitatory synaptic current flow into dendrites (28), and 3) presynaptic fiber volleys (FVs), which reflect the number of activated axons (28). Stimulation of the MPP evoked significantly larger PSs in slices taken from EC rats. There was no significant effect of rearing environment on PS/EPSP relationships. The slopes of EPSPs recorded at the site of synaptic activation in the dentate molecular layer and at the major current source in the dentate granule cell layer were significantly greater in slices taken from EC rats. The presynaptic FV was recorded at the site of synaptic activation in the molecular layer. FV amplitude did not differ significantly as a function of rearing environment. To examine possible differences in tissue impedance, granule cells were activated by stimulating granule cell axons in the dentate hilus and recording the antidromic PS in the granule cell layer. Antidromic PS amplitude was not significantly affected by rearing environment. The relative permanence of the experience-dependent alterations in synaptic transmission was assessed by comparing slices taken from rats that had been reared for 4 wk in complex environments followed by 3-4 wk in individual cages with those from rats reared for 7-8 wk in individual cages. There were no significant differences in MPP synaptic transmission between these groups of animals. The results suggest that experience in a relatively complex environment is associated with greater MPP synaptic transmission arising from an increased synaptic input to granule cells; the greater MPP synaptic transmission associated with behavioral experience can occur independent of behavioral state, influences from extrahippocampal brain regions and intrahippocampal inhibitory activity; and the experience-dependent synaptic alterations in the dentate gyrus are transient, in contrast to experience-dependent morphological alterations described in occipital cortex. The possible relationship of these alterations to the phenomenon of long-term enhancement is discussed.

Effect of sodium selenite on growth, insulin-like growth factor-binding proteins and insulin-like growth factor-I in rats

1995 ◽  
Vol 145 (1) ◽  
pp. 105-112 ◽  
Author(s):  
H Grønbæk ◽  
J Frystyk ◽  
H Ørskov ◽  
A Flyvbjerg
Keyword(s):  
Growth Factor ◽  
Sodium Selenite ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Igf I ◽  
Selenium Treatment ◽  
Group A ◽  
Ad Libitum ◽  
Group B ◽  
Igfbp 3

Abstract Selenium is an essential trace element although at higher doses it is also known to be a toxic agent causing a wide range of symptoms including growth retardation. In order to investigate the effect of sodium selenite on growth, insulin-like growth factor-binding proteins (IGFBPs) and insulin-like growth factor-I (IGF-I), 30 male Wistar rats were randomized into three groups. Group A was treated with sodium selenite in the drinking water (3·3 mg selenium/l). Group B was ad libitum fed with free access to standard fodder and tap water and group C was pair fed relative to the selenium-treated rats. Serum IGF-I and IGFBPs were determined on days 0, 14 and at the end of the study on day 35. Selenium-treated rats had significantly lower body weights compared with group B rats on day 9 and group C rats on day 14 (P<0·05). Tibia length was measured at the end of the study and no difference was observed between groups B and C (3·77 ± 0·04 cm vs 3·60±0·02 cm); however, selenium-treated rats had significantly shorter tibia lengths (3·46±0·03 cm) compared with rats in groups B (P<0·001) and C (P<0·05). Selenium treatment induced a significant reduction in circulating IGF-I by the end of the study compared with ad libitum and pair fed rats (P<0·05). Serum subjected to Western ligand blots showed four distinct IGFBP bands with apparent relative molecular weights of 38–47 kDa (doublet) (IGFBP-3), 30 kDa (IGFBP-1 and/or IGFBP-2) and 24 kDa (IGFBP-4). At the end of the study a significant reduction in IGFBP-3 was observed in group A compared with groups B and C (P<0·05). Selenium treatment also caused a reduction in IGFBP-1 and/or IGFBP-2 compared with ad libitum fed rats; in addition, a reduction was observed in pair fed controls. In conclusion, sodium selenite treatment leads to growth retardation accompanied by reduced circulating levels of IGF-I, IGFBP-3, and IGFBP-1 and/or IGFBP-2. The reduction in IGF-I and IGFBP-3 could not be attributed to reduced caloric intake but seems to be a specific action of selenium. Journal of Endocrinology (1995) 145, 105–112

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