scholarly journals Phytochemical content and antioxidant activities of Rhubarb (Rheum emodi)

2021 ◽  
Author(s):  
Ahmed Shahwan ◽  
Naema El Aali ◽  
Yusra Layas ◽  
Salem G. El Tumi
Keyword(s):  
Antioxidant Activities ◽  
Water Extract ◽  
Reducing Power ◽  
Total Phenolic ◽  
Phytochemical Content ◽  
Reducing Ability ◽  
Reducing Power Assay ◽  
Novel Antioxidants ◽  
Phenolic And Flavonoid ◽  
Phenolic And Flavonoid Compounds

Medicinal plants have varying natural products and several antioxidants. Antioxidants play a principal function to protect against destruction caused by oxidative stress (OS), <i>Rheum emodi</i> is not an exception, in which it is reported to have compounds that possess antioxidant activity, like polyphenolic compounds. In addition to that, other compounds have proven to have antidiabetic, antimicrobial, antifungal, cytotoxic, hepatoprotective, and nephroprotective activities. This study aimed to quantify the water extract of a powdered plant of <i>R. emodi</i> to evaluate its ability to scavage free radicals. Total phenolic, total flavonoid contents, and reducing ability were measured to consider possible sources of future novel antioxidants in plants. The TPC, TFC, and reducing power assay increased with increasing concentrations of <i>R. emodi</i>. At 1000 µg/ml, the absorbance ranged from 1.437 for TPC, 1.602 for TFC, and 1.638 for reducing power assay, which is more than the absorbance of the standards at the same concentration. The extracted content of reducing power, phenolic, and flavonoid compounds were higher than the standard pyrogallol, quercetin, and ascorbic acid.

Effect of gamma irradiation processing on total phenol and antioxidant capacities of the Iranian extract of propolis

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Marzieh Heidarieh ◽  
Ali Nabipour Chakoli ◽  
Samira Shahbazi ◽  
Parvin Shawrang ◽  
Baode Zhang
Keyword(s):  
Gamma Irradiation ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
The Other ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Flavonoid Compounds ◽  
Gamma Irradiated ◽  
Phenolic And Flavonoid ◽  
Phenolic And Flavonoid Compounds

Abstract The Iranian ethalonic extract of propolis (EEP) were analyzed to determine their antioxidant activities and total phenolic and flavonoid contents, with and without gamma irradiation processing under various dosage of gamma (10, 20, 30, 40 and 50 kGy). The analysis results show that the gamma irradiated EEP at 10 KGy can scavenge free radicals and exhibit higher antioxidant-reducing power to the other groups (P < 0.05). It is noted that gamma irradiated EEP has higher total phenolic contents compared to gamma irradiated EEP at 30, 40 and 50 KGy and non-irradiated EEP (P < 0.05). The flavonoid compounds as indicated by C-13 NMR analysis were found to be higher in gamma irradiated EEP at 10 kGy compared with the other groups. In conclusion, gamma irradiation processing of EEP causes can enhanced antioxidant activities phenolic and flavonoid compounds at dose of 10 KGy.

scholarly journals Antifungal, Antibacterial, and Antioxidant Activities of Acacia Saligna (Labill.) H. L. Wendl. Flower Extract: HPLC Analysis of Phenolic and Flavonoid Compounds

Molecules ◽  
2019 ◽  
Vol 24 (4) ◽  
pp. 700 ◽  
Author(s):  
Asma Al-Huqail ◽  
Said Behiry ◽  
Mohamed Salem ◽  
Hayssam Ali ◽  
Manzer Siddiqui ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Water Extract ◽  
Wood Products ◽  
Butylated Hydroxytoluene ◽  
Acacia Saligna ◽  
Flower Extract ◽  
Flavonoid Compounds ◽  
Carotovorum Subsp ◽  
Phenolic And Flavonoid ◽  
Phenolic And Flavonoid Compounds

In this study, for the environmental development, the antifungal, antibacterial, and antioxidant activities of a water extract of flowers from Acacia saligna (Labill.) H. L. Wendl. were evaluated. The extract concentrations were prepared by dissolving them in 10% DMSO. Wood samples of Melia azedarach were treated with water extract, and the antifungal activity was examined at concentrations of 0%, 1%, 2%, and 3% against three mold fungi; Fusarium culmorum MH352452, Rhizoctonia solani MH352450, and Penicillium chrysogenum MH352451 that cause root rot, cankers, and green fruit rot, respectively, isolated from infected Citrus sinensis L. Antibacterial evaluation of the extract was assayed against four phytopathogenic bacteria, including Agrobacterium tumefaciens, Enterobacter cloacae, Erwinia amylovora, and Pectobacterium carotovorum subsp. carotovorum, using the micro-dilution method to determine the minimum inhibitory concentrations (MICs). Further, the antioxidant capacity of the water extract was measured via 2,2′-diphenylpicrylhydrazyl (DPPH). Phenolic and flavonoid compounds in the water extract were analyzed using HPLC: benzoic acid, caffeine, and o-coumaric acid were the most abundant phenolic compounds; while the flavonoid compounds naringenin, quercetin, and kaempferol were identified compared with the standard flavonoid compounds. The antioxidant activity of the water extract in terms of IC50 was considered weak (463.71 μg/mL) compared to the standard used, butylated hydroxytoluene (BHT) (6.26 μg/mL). The MIC values were 200, 300, 300, and 100 µg/mL against the growth of A. tumefaciens, E. cloacae, E. amylovora, and P. carotovorum subsp. carotovorum, respectively, which were lower than the positive control used (Tobramycin 10 μg/disc). By increasing the extract concentration, the percentage inhibition of fungal mycelial was significantly increased compared to the control treatment, especially against P. chrysogenum, suggesting that the use of A. saligna flower extract as an environmentally friendly wood bio-preservative inhibited the growth of molds that cause discoloration of wood and wood products.

scholarly journals PHYTOCHEMICAL CONTENT AND ANTIOXIDANT POTENTIAL OF DIFFERENT ORGANS OF EGGPLANT (SOLANUM MELONGENA L.) GROWN IN WEST JAVA-INDONESIA

2017 ◽  
Vol 10 (8) ◽  
pp. 144
Author(s):  
Irda Fidrianny ◽  
Siti Winarsih ◽  
Komar Ruslan
Keyword(s):  
Antioxidant Activities ◽  
Solanum Melongena ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Antioxidant Power ◽  
Phytochemical Content ◽  
Fruit Extracts ◽  
Dpph Method ◽  
Phenolic And Flavonoid

  Objectives: The goals of this research were to evaluate antioxidant potential from different organs of eggplant (Solanum melongena L.) using two antioxidant testing methods which were 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) and correlation of total phenolic and flavonoid content with their inhibitory concentration 50% (IC50) of DPPH, and exhibitory concentration 50% (EC50) of FRAP.Materials and Methods: Each sample was extracted by reflux using different polarity solvents. The extracts were evaporated using rotary evaporator. Antioxidant activities were tested using DPPH and FRAP assays, determination of total phenolic and flavonoid content were carried out by ultraviolet-visible spectrophotometry and correlation with their IC50 of DPPH and EC50 of FRAP capacities were analyzed by Pearson’s method.Results: The lowest IC50 of DPPH scavenging activity 1.14 μg/ml and the lowest EC50 of FRAP capacity 49.80 μg/ml was given by ethanolic leaves extract of eggplant. Ethanolic leaves extract of eggplant also presented the highest total phenolic content (TPC) (8.87 g gallic acid equivalent/100 g), while the highest total flavonoid content was shown by ethyl acetate leaves extract (24.50 g quercetin equivalent/100 g). There was a significantly negative correlation between TPC in leaves and fruit extracts of eggplant with their IC50 of DPPH and EC50 of FRAP.Conclusions: All different extracts of eggplant organs (except n-hexane stem extract) were categorized as a very strong antioxidant by DPPH method. Phenolic compounds in eggplant leaves and fruit extracts were the major contributor in antioxidant activities by DPPH and FRAP methods. DPPH and FRAP showed linear results in antioxidant activities of eggplant leaves, fruit and stem extracts.

scholarly journals EVALUATION OF THREE METHODS FOR THE EXTRACTION OF ANTIOXIDANTS FROM VICIA FABA L. BEAN AND HULLS

2014 ◽  
Vol 44 (3) ◽  
pp. 203-208
Author(s):  
Z. HASHEMI ◽  
M. A. EBRAHIMZADEH
Keyword(s):  
Vicia Faba ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Extraction Methods ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Assisted Extraction ◽  
Vicia Faba L ◽  
Phenolic And Flavonoid

 The efficiencies of three extraction methods (percolation, Soxhlet and ultrasonically assisted extraction) were evaluated for the extraction of antioxidants from Vicia faba L. bean and hulls. Antioxidant activities of extracts were evaluated using four different tests. Total phenolic and flavonoid contents of the extracts were determined using the Folin–Ciocalteu and aluminium chloride methods, respectively. Although the yield of extractions for ultrasonically assisted extraction was about half to one-fourth of that of other methods, the extraction ratio of total phenol was higher. Hull extracts had higher total phenolic and flavonoid contents and antioxidant activities than bean extracts. The hull ultrasonic extract showed the best DPPH (IC50= 56.9 ± 2.5 g ml-1) and NO radical scavenging (11.3 ± 0.5 gml-1). The hull percolation extract showed the best iron chelating ability (171.8 ± 6.8 g ml-1) and reducing power. The results indicated that all extraction methods could effectively extract antioxidants from medicinal plants

scholarly journals ANTIOXIDATIVE AND FREE RADICAL SCAVENGING POTENTIALS OF CYCLOSORUS INTERRUPTUS (WILLD.) H. ITÔ AND PRONEPHRIUM NUDATUM (ROXB. EX GRIFF.) HOLTTUM

2019 ◽  
pp. 87-94
Author(s):  
ABHIJIT MITRA ◽  
MANABENDRA DUTTA CHOUDHURY ◽  
PRAKASH ROY CHOUDHURY ◽  
DEEPA NATH ◽  
SUBRATA DAS ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
In Vitro Systems ◽  
Reducing Power Assay ◽  
Phenolic And Flavonoid

Objectives: The work aims to screen the antioxidative potentials of different crude extracts of the fronds of two medicinally important pteridophytes of Southern Assam, India, namely, Cyclosorus interruptus (Willd.) H. Itô and Pronephrium nudatum (Roxb. ex Griff.) Holttum. Methods: Frond extracts of the pteridophytes were prepared by Soxhlet hot extraction method. Total phenolic content (TPC) and total flavonoid content (TFC) of the hexane, ethyl acetate, acetone, and methanol extracts of the fronds of the plants were done by following standard protocol. In vitro assessment of the antioxidative behavior of the extracts was performed using standard 2,2-diphenyl-1-picrylhydrazyl scavenging assay, reducing power assay, metal chelating assay, hydroxyl, superoxide, and 2,2’- azino-bis (3-ethylbenzothiazoline - 6 – sulfonic acid) radical scavenging methods in different in vitro systems. Results: Preliminary phytochemical analysis implicated the presence of phenolic and flavonoid compounds in all the frond extracts. The methanol extract of the fronds of both the plants showed maximum phenolic and flavonoid contents in comparison to the other extracts, however, that of C. interruptus was found to be higher than P. nudatum. Antioxidative potentials of the said extracts were also found to be impressive and noteworthy. The decreasing order of the antioxidative efficacies of the extracts was found to be same as that of TPC and TFC of the extracts. Conclusion: It is pertinent to comment that the methanol extract of the fronds of both the plants may be treated as a potential source of natural antioxidants.

scholarly journals EKSTRAKSI KOMPONEN ANTIOKSIDAN TEPUNG KOMPOSIT BERBASIS PISANG GOROHO DAN LABU KUNING DENGAN BANTUAN ULTRASONIK

2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Immanuel M. Pasanda ◽  
Edi Suryanto ◽  
Adisti A. Rumayar
Keyword(s):  
Antioxidant Capacity ◽  
Reducing Power ◽  
Beta Carotene ◽  
Total Phenolic ◽  
Composite Flour ◽  
Carotene Content ◽  
Phytochemical Content ◽  
Antioxidant Content ◽  
Composite Flours ◽  
Reducing Power Assay

ABSTRAK Tanaman-tanaman lokal yang mengandung antioksidan, yaitu pisang goroho dan labu kuning, digunakan untuk mengembangkan tepung komposit.  Penelitian dilakukan untuk mengevaluasi efek pencampuran tepung pisang goroho dengan tepung labu kuning terhadap kandungan fitokimia antioksidan (total fenolik dan beta karoten) dan kapasitas antioksidan (kemampuan menangkal radikal DPPH dan daya reduksi) dari tepung komposit yang dihasilkan dengan menggunakan bantuan ultrasonik pada tahapan ekstraksi. Formulasi tepung komposit yang digunakan (tepung pisang goroho:tepung labu kuning) adalah 75:25, 50:50, dan 25:75. Kandungan total fenolik tepung komposit berkisar antara 98,2- 78,8 mg/kg GAE, dan kandungan beta karoten tepung komposit berkisar antara 186,1-497,8 µg/g. Hasil penelitian menunjukkaan peningkatan  kandungan fitokimia antioksidan dan kapasitas antioksidan pada tepung komposit terjadi dengan meningkatnya proporsi tepung labu kuning. ABSTRACT Locally grown crops with antioxidant content i.e. goroho plantain dan yellow pumpkin were used to develop composite flours. The current study aimed to investigate the effect of mixing goroho plantain flour with yellow pumpkin flour on phytochemical content (total phenolic and beta carotene) and antioxidant capacity (DPPH assay and reducing power assay) of developed composite flours, assisted by ultrasound in the extraction step. Flour formulations (goroho plantain flour: yellow pumpkin flour) used were 75:25, 50:50, and 25:75.  Total phenolic content of composite flours ranged from 98.2-178.8 mg/kg GAE, and beta carotene content of composite flours ranged from 186,1-497,8 µg/g. The study showed that increased in phytochemical antioxidant content and antioxidant capacity of composite flours were due to increasing in yellow pumpkin flour proportion in composite flour. 

scholarly journals Phenolic compounds profile, neuroprotective effect and antioxidant potential of a commercial Turkish coffee

2020 ◽  
Vol 33 ◽  
Author(s):  
Melek ÇOL AYVAZ
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Neuroprotective Effect ◽  
Radical Scavenging ◽  
Water Extract ◽  
Reducing Power ◽  
Total Phenolic ◽  
Scavenging Activity

ABSTRACT Objective The purpose of this study is to determine the phenolic and flavonoid contents, and antioxidant activities and neuroprotective effects of powdered coffee sample of a commercial coffee brand originated from Sivas, Turkey. Methods Total phenolic, flavonoid and antioxidant contents, enzymatic and non-enzymatic antioxidative activities based on 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity, metal chelating potential, reducing power, superoxide dismutase and catalase activity tests and lipid peroxidation inhibition potentials of the ethanolic and aqueous extracts of the coffee sample were assayed using the commonly preferred spectrophotometric methods. Furthermore the extracts’ cholinesterase and tyrosinase inhibition potentials were evaluated. Phenolic profiles of the coffee sample were investigated using high performance liquid chromatography. Results Catechin was the most frequently detected phenolic acid. In addition, it was demonstrated that the water extract has a significant impact when compared with standard antioxidants. While the SC50 (sufficient concentration to obtain 50% of a maximum scavenging capacity) value for the scavenging activity of 2,2-diphenyl-1-picrylhydrazyl free radical was calculated as being 0.08mg/mL for water extract, the amount of chelating agents with half Fe2+ ions in the medium was found to be 0.271mg/mL. Additionally, it was shown that 0.1mg/mL concentration of both extracts prevents lipid peroxidation by 8%. Compared with standard drugs, inhibition potentials of cholinesterase and tyrosinase enzymes were considered as moderately acceptable in these samples. Conclusion Besides the extracts’ enzymatic antioxidant activity, their inhibition potential on cholinesterase and tyrosinase enzymes – which are important clinical enzymes – reveal that this natural source can be used as a valuable resource in different fields, especially in medicine.

scholarly journals TOPICAL APPLICATION OF TERMINALIA CHEBULA CREAM INHIBITS ULTRAVIOLET-B-INDUCED PSORIASIS IN RATS

2020 ◽  
pp. 15-20
Author(s):  
Aruna Pai ◽  
Jvln Seshagiri Rao ◽  
Madhu J Rajendra ◽  
Ashish S Jain
Keyword(s):  
Topical Application ◽  
Water Extract ◽  
Reducing Power ◽  
Ultraviolet B ◽  
Total Phenolic ◽  
Terminalia Chebula ◽  
Epidermal Thickness ◽  
Reducing Ability ◽  
Dpph Method ◽  
Anti Oxidant

Introduction: The present study aims at evaluating anti oxidant activity and inhibition of topical application of Terminalia chebula cream in ultraviolet –B-induced psoriasis in rats. Materials &Methods: The seeds of Terminalia chebula were dried and powder was extracted with chloroform, ethyl acetate, ethanol, methanol, and deionized water by cold maceration method. Total phenolic content, total tannin content and total triterpenoid content was determined by different methods. Anti oxidant activity was evaluated by DPPH method and reducing power assay. Cream formulation of the optimized extract was prepared by hot fusion method. Psoriasis was induced by irradiatiating for 30 min at a vertical distance of 20 cm with UV light (385 nm) on the skin. Severity index, hydroxyproline content and epidermal thickness was determined. Results and Discussion: Total Phenol (mg gallic acid/ g) content, Total Tannins (mg catechin/ g) content and  Total triterpenoid (mg ursolic acid/ g) content was found to be significant for water extract.The reducing ability of water extract was always higher than methanol extract at all the concentrations. Conclusion: We concluded that cream containing 0.1% water extract of Terminalia chebula seeds possess antioxidant activity along with potent antipsoriatic activity. Further, application of Terminalia chebula cream resulted in decrease in relative epidermal thickness of animal skin as well as other histopathological features. The present investigation aims at the development of potent phytomedicine for treatment of psoriasis from the Terminalia chebula plant.

scholarly journals Concordance of antioxidant and anti-Inflammatory activity in Xylocarpus granatum (Koen)

2019 ◽  
Vol 17 (4) ◽  
pp. 466-475
Author(s):  
Md Emdadul Islam ◽  
SM Mahbubur Rahman ◽  
Md Hossain Sohrab ◽  
Rana Biswas ◽  
Mohammad Shaef Ullah ◽  
...  
Keyword(s):  
Reducing Power ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Rbc Membrane ◽  
A Value ◽  
Anti Inflammatory ◽  
Reducing Power Assay ◽  
Phenolic And Flavonoid ◽  
Β Carotene

Xylocarpus granatum, a common mangrove plant is traditionally used for the treatment of diarrhoea, cholera, fever, dyslipidemia, inflammation, etc. The present study was carried out to evaluate the antioxidant and anti-inflammatory potential of the ethanolic extract of X. Granatum by various in vitro methods such as 1,1-diphenyl-2-picryl-hydrazil (DPPH) free radical scavenging assay,  reducing power assay, ferric reducing antioxidant power (FRAP) and β-carotene bleaching inhibition assay. Total phenolic and flavonoid content were determined. Anti-inflammatory activity was evaluated by in vitro human RBC membrane stabilizing assay and in vivo mice paw edema test. Ethanolic leaf extract (S2) of X. granatum showed significant scavenging effect on DPPH scavenging with a value of IC50 165.95µg/ml. In addition, it showed significant reducing potential with a value of 59.04 mM of ferrous equivalent per ml in FRAP assay and in reducing power assay the EC50 value was determined as 241.61μg/ml . The ethanolic leaf extracts exhibited 72.3% β-carotene bleaching inhibition. The total phenolic and flavonoid content of the extract were 66μg/ml gallic acid equivalent and 47.66μg/ml quercetin equivalent per gram of dry extract, respectively. The extract also exhibited 52.63% and 51.05% protection of RBC membrane in hypotonicity and heat induced lysis inhibition, respectively. Significant reduction of mice paw edema (36.34% in 20 μg/kg bw concentration) was observed in the extract. The results revealed that the leaf extract of X. granatum possesses strong antioxidant and anti-inflammatory potential. J Bangladesh Agril Univ 17(4): 466–475, 2019

scholarly journals SettingsTOPICAL APPLICATION OF TERMINALIA CHEBULA CREAM INHIBITS ULTRAVIOLET-B-INDUCED PSORIASIS IN RATS

2020 ◽  
Vol 3 (3) ◽  
pp. 15-20
Author(s):  
Aruna Pai ◽  
Jvln Seshagiri Rao ◽  
Madhu Rajendra ◽  
Ashish Jain
Keyword(s):  
Water Extract ◽  
Reducing Power ◽  
Severity Index ◽  
Ultraviolet B ◽  
Total Phenolic ◽  
Terminalia Chebula ◽  
Epidermal Thickness ◽  
Reducing Ability ◽  
Dpph Method ◽  
Anti Oxidant

Introduction: The present study aims at evaluating anti oxidant activity and inhibition of topical application of Terminalia chebula cream inultraviolet –B-induced psoriasis in rats.Materials and Methods: The seeds of Terminalia chebula were dried and powder was extracted with chloroform, ethyl acetate, ethanol,methanol, and deionized water by cold maceration method. Total phenolic content, total tannin content and total triterpenoid content wasdetermined by different methods. Anti oxidant activity was evaluated by DPPH method and reducing power assay. Cream formulation of theoptimized extract was prepared by hot fusion method. Psoriasis was induced by irradiatiating for 30 min at a vertical distance of 20 cm withUV light (385 nm) on the skin. Severity index, hydroxyproline content and epidermal thickness was determined.Results: Total Phenol (mg gallic acid/ g) content, Total Tannins (mg catechin/ g) content and Total triterpenoid (mg ursolic acid/ g) contentwas found to be significant for water extract.The reducing ability of water extract was always higher than methanol extract at all theconcentrations.Conclusion: We concluded that cream containing 0.1% water extract of Terminalia chebula seeds possess antioxidant activity along withpotent antipsoriatic activity. Further, the application of Terminalia chebula cream resulted in decrease in relative epidermal thickness of animalskin as well as other histopathological features. The present investigation aims at the development of potent phytomedicine for treatment ofpsoriasis from the Terminalia chebula plant.

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