scholarly journals SLOW GROWTH IN VITRO CULTURE FOR CONSERVATION OF HANCORNIA SPECIOSA GOMES

FLORESTA ◽  
2022 ◽  
Vol 52 (1) ◽  
pp. 007
Author(s):  
Kívia Soares de Oliveira ◽  
Magdi Ahmed Ibrahim Aloufa
Keyword(s):  
Generalized Linear Model ◽  
Slow Growth ◽  
Ms Medium ◽  
Leaf Color ◽  
Culture Time ◽  
Fruit Species ◽  
Significant Difference ◽  
Number Of Leaves ◽  
Hancornia Speciosa

Hancornia speciosa Gomes is a fruit species endemic to the Cerrado and coastal plains of Northeast of Brazil, with great economic, nutritional, ecological, and medicinal potential. This study aimed to evaluate the effect of sorbitol and sucrose as osmotic regulators on the in vitro growth of mangabeira, aiming at conservation by slow growth. The explants were obtained from in vitro germinated seedlings and inoculated in MS medium supplemented with sucrose (15 and 30 g L-1) and sorbitol (0, 5, 10 and 15 g L-1). The experimental design was completely randomized with 20 repetitions in a 4 x 2 factorial arrangement (sorbitol x sucrose concentrations). The evaluations were performed at 30, 60 90 and 120 days of incubation. The analyzed variables were number of nodes/budding, number of leaves, leaf abscission, leaf color and survival of explants. The data were statistically analyzed by generalized linear model analysis. The results indicated a significant difference between the osmotic regulators and the culture time for all variables. Sorbitol showed a more pronounced growth-reducing effect than sucrose. The use of 30 g L-1 sucrose combined with 10 or 20 g L-1 sorbitol reduced the growth in a critical way, making it clear that the water stress caused was not tolerated by the plants, negatively interfering in its development. Treatment with 15 g L-1 sucrose combined with 5 g L-1 sorbitol promoted the best result, allowing the conservation of plants for 120 days.

scholarly journals The Effect of Kinetin Concentration on Eucalyptus pellita F. Muell Micro Cutting Growth (In Vitro)

2019 ◽  
Vol 1 (1) ◽  
pp. 34
Author(s):  
Ridho Dzikrana
Keyword(s):  
Leaf Color ◽  
Culture Techniques ◽  
Eucalyptus Pellita ◽  
Randomized Design ◽  
Significant Difference ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Single Factor Experiment ◽  
Tissue Culture Techniques

Propagation of quality, uniform, and large quantities of Eucalyptus pellita F. Muell seeds as one type of industrial forest plant can be done by tissue culture techniques.  The aim of the study was to determine: 1) the effect of  kinetin concentration on the growth of E. pellita micro cuttings; 2) kinetin concentration which gives the best effect on the growth of E. pellitamicro cuttings.  A single factor experiment, kinetin concentration, was arranged in a Completely Randomized Design (CRD), consisted of five treatments, namely 0; 1; 2; 3; and 4 mg of kinetin L-1 and each treatment was replicated five times.  The variables observed consisted of plant height, leaf color, and number of leaves.  Data were analyzed by analysis of variance (anova) and if the anova was significantly different, to compare between the two treatment averages, followed by a Least Significant Difference  (LSD) test at the level of 5%.  The results showed that different kinetin concentrations had different effects on the height of the culture and number of leaves, but the color of the leaves did not show significant different.  A concentration of  3 mg of kinetin L-1 gave the best effect on the height cuttings at all observation ages: 7; 14; 21; 28; 35; 42; and 49 days after inoculation (DAI) and number of leaves.

scholarly journals Plantlet Regeneration and Multiple Shoot Induction from Protocorm-Like Bodies (PLBs) of Medicinal Orchid Species, Dendrobium crumenatum Sw.

2021 ◽  
Vol 18 (7) ◽  
Author(s):  
Sutha KLAOCHEED ◽  
Suphat RITTIRAT ◽  
Kanchit THAMMASIRI
Keyword(s):  
Culture Media ◽  
Multiple Shoot ◽  
Ms Medium ◽  
Multiple Shoot Induction ◽  
Orchid Species ◽  
Plantlet Growth ◽  
Significant Difference ◽  
Number Of Leaves ◽  
Medicinal Orchid

To investigate the suitable medium for in vitro shoot regeneration and plantlet growth of Dendrobium crumenatum Sw., individual protocorm-like bodies (PLBs) (about 4 - 5 mm in diameter) of Dendrobium crumenatum Sw. derived from MS medium supplemented with 0.5 mg/L TDZ for 60 days of culture were cultured on 6 culture media; Murashige and Skoog (MS) medium, MS medium supplemented with 15 % (v/v) CW, MS medium supplemented with 15 % (v/v) CW and 0.2 % (w/v) AC, Vacin and Went (VW) medium, VW medium supplemented with 15 % (v/v) CW, VW medium supplemented with 15 % (v/v) CW and 0.2 % (w/v) AC. After 4 months of culture, MS medium containing 15 % coconut water (CW) gave the highest percentage of shooting and number of shoots per explant of 96.0 and 9.5, respectively with a significant difference from other media. The addition of 0.2 % (w/v) activated charcoal (AC) significantly increased the number of leaves and roots. PLBs developed into complete plantlets. MS medium supplemented with 15 % (v/v) CW and 0.2 % (w/v) AC and VW medium supplemented with 15 % (v/v) CW and 0.2 % (w/v) AC gave the highest number of roots per plantlet and root length at 5.3 roots and 34.9 mm, respectively. After the transfer of rooted shoots to the greenhouse, 95 % of the regenerated plantlets survived and grew vigorously. Plantlets grown in vitro were successfully acclimatized in the greenhouse and showed normal development.

scholarly journals GROWTH OF ‘PRATA-ANA’ BANANA’S MICROSHOOTS CLONE GORUTUBA FROM SYNTHETIC SEEDS:SUBSTRATES AND BAP CONCENTRATION

2017 ◽  
Vol 39 (5) ◽  
Author(s):  
JÚLIO CÉSAR GOMES PEREIRA ◽  
SELMA SILVA ROCHA ◽  
LUCIANA CARDOSO NOGUEIRA LONDE ◽  
MARCELA CAROLINE BATISTA DA MOTA ◽  
PABLO FERNANDO SANTOS ALVES ◽  
...  
Keyword(s):  
Culture Medium ◽  
Economic Importance ◽  
Leaf Length ◽  
Naphthalene Acetic Acid ◽  
Synthetic Seed ◽  
Ms Medium ◽  
World Production ◽  
Number Of Leaves ◽  
Alginate Matrix

ABSTRACT The banana crop stands out as an activity of great social and economic importance in Brazil, which occupies the fifth place in world production. Synthetic seed production is becoming promising for a micropropagation and in vitro conservation. The aim of the study was to analyze the conversion and growth of ‘Prata-anã’ banana’s microshoots clone Gorutuba from synthetic seed in MS medium and vermiculite, different substrates and concentrations of BAP (6-benzylaminopurine) associated with ANA (acetic naphthalene acid) in the constitution of its capsule were tested. The microshoots were immersed in the sodium alginate matrix (3%) and dripped in a solution of CaCl2.2H2O (100 mM) for complexation and then in KNO3 solution (100 mM) to decomplex. The experimental design was completely randomized in a 2 x 5 factorial design (substrate x BAP concentrations), containing different substrates (MS culture medium and vermiculite) and BAP concentrations (2.22, 4.44, 6.66, 8.88 and 13.32 µmol L-1) associated with NAA (naphthalene acetic acid) 0.54 µmol L-1, totaling 10 treatments, with 4 replicates, and that each replicate containing 5 seeds. The evaluations of conversion, number of leaves, leaf length, leaf height, number of roots, root length and oxidation were performed at 30 and 60 days.The use of the MS medium provided better growth results in relation to vermiculite as substrate, in which the different BAP concentrations did not differ from each other. It was found that, in MS culture medium, BAP concentrations above 8.88 µmol L-1 in the capsule composition are not indicated for microshoots growth.

scholarly journals Impact of saline solution on growth and photosystem II during in vitro cultivation of Bromelia antiacantha (Bromeliaceae)

Rodriguésia ◽  
2021 ◽  
Vol 72 ◽  
Author(s):  
Rosiane Cipriano ◽  
João Paulo Rodrigues Martins ◽  
Luiz Carlos de Almeida Rodrigues ◽  
Antelmo Ralph Falqueto ◽  
Andreia Barcelos Passos Lima Gontijo
Keyword(s):  
Photosystem Ii ◽  
Saline Solution ◽  
Growth Traits ◽  
Photosynthetic Apparatus ◽  
Solution Culture ◽  
In Vitro Cultivation ◽  
Ms Medium ◽  
Number Of Leaves ◽  
Positive Effect

Abstract In vitro cultivation is a technique with wide application for micropropagation. However, each species has specific mineral needs for this type of cultivation. The objective was to assess the impacts of the saline solution culture medium on the performance of the photosynthetic apparatus and growth of Bromelia antiacantha during in vitro cultivation, and thus to elucidate the mitigation of the nutritional imbalance that can interfere in the electron transport in the plants. Plants were cultivated in a salt concentration gradient of MS medium (0%, 25%, 50%, 75% or 100%). The growth traits and fluorescence a chlorophyll were analyzed. Intermediate concentrations of MS medium resulted in plants with a larger number of leaves and longer root length. The OJIP curves and results of the JIP test showed that the plants grown without MS salts presented less efficient photosystem II (PSII), as indicated by the performance index [Pi(total)]. In contrast, the intermediate concentrations (MS 25% and 50%) had a positive effect on the performance of the photosynthetic apparatus. The MS 25% medium can be used for in vitro cultivation of B. antiacantha, enabling the development of plants with suitable physiological qualities for planting in the field.

scholarly journals The Effect of Slow-grown in Vitro Storage Under Different Light Spectra on Banana Plantlets Cv. Prata Catarina (AAB).

2021 ◽  
Author(s):  
Paulo Hercilio Viegas Rodrigues ◽  
Emerson Oliveira ◽  
Christian Demetrio ◽  
Guilherme Ambrosano ◽  
Sônia Maria Stefano Piedade
Keyword(s):  
Plant Material ◽  
Slow Growth ◽  
Shoot Cultures ◽  
Ms Medium ◽  
In Vitro Storage ◽  
Light Spectra ◽  
Slow Growth Storage ◽  
C 50 ◽  
Commercial Micropropagation

Abstract Maintaining updated in vitro plant subcultures is essential for commercial micropropagation and tissue culture research. In unusual situations, the subcultures can be delay and the slow-growth in vitro storage technic could be applied to reduce the loss of plant material. The present study aimed to evaluate the slow-growth in vitro storage of banana plantlets (‘Prata Catarina’; group AAB) under different light spectra. Shoot cultures in MS medium without plant growth regulators were maintained under blue (B), red (R), red plus blue (R2B), and white (CW) light spectra (25°C ± 2°C; 50 µmol m -2 s -1 ) for up to 140 days. The plantlets maintained under the R, CW, and R2B spectra did not survive after 140 days of in vitro slow-growth storage. The plantlets maintained under the B spectrum survived after 140 days of in vitro slow-growth storage and showed little browning.

scholarly journals INDUKSI KALUS PASAK BUMI (Eurycoma longifolia Jack) MELALUI EKSPLAN DAUN DAN PETIOL

2016 ◽  
Vol 6 (1) ◽  
pp. 33
Author(s):  
ROSMAINA ROSMAINA ◽  
ZULFAHMI ZULFAHMI ◽  
PROBO SUTEJO ◽  
ULFIATUN ULFIATUN ◽  
MAISUPRATINA MAISUPRATINA
Keyword(s):  
Slow Growth ◽  
Callus Formation ◽  
Germination Percentage ◽  
Ms Medium ◽  
Petiole Explants ◽  
Eurycoma Longifolia ◽  
Yellow Color ◽  
Eurycoma Longifolia Jack ◽  
Short Time

One of the problem of Eurycoma longifolia Jack propagation was low germination percentage due to recalcitrant seeds and slow growth of seedling from cutting propagation. To overcome this problem is required propagation of Eurycoma longifolia via in vitro culture. The objective of this research was to know the effect of Auxin (2,4-D and NAA) and Cytokines (BAP and Kinetin)  on Eurycoma longifolia callus induction via leaf and petiole explants. In this study, we used plant growth regulator of 2,4 D, NAA, BAP and Kinetin in several levels.  The observed variables were appearing callus time, callus color and callus texture. The results of this study showed that MS medium supplemented with 1 ppm NAA+ 1 ppm BAP was able to induce callus formation in leaf explant for 6 months after culture. While MS medium supplemented with 1 ppm 2,4-D, 1 ppm BAP, combination of 2,4-D and Kinetin and combination of 2,4-D and BAP can induce callus formation from petiole. All the callus formation has yellow color and yellow brown color. The petiole explant that is grown in MS medium supplemented with 1 ppm BAP induced of callus in short time (18 days after culture).

scholarly journals In vitro growth and development of Dendrobium hybrid orchid

1970 ◽  
Vol 35 (3) ◽  
pp. 507-514 ◽  
Author(s):  
H Khatun ◽  
MM Khatun ◽  
MS Biswas ◽  
MR Kabir ◽  
M Al-Amin
Keyword(s):  
Growth Regulators ◽  
Growth And Development ◽  
Ms Medium ◽  
Fresh Weight ◽  
Maximum Weight ◽  
Shoot Height ◽  
Single Shoot ◽  
Number Of Leaves ◽  
Regenerated Plantlets

The experiment was conducted to investigate the combined effect of different plant growth regulators and charcoal supplementation in MS medium on growth and development of plantlets regenerated from protocorm like bodies (PLBs) of hybrid orchid. The combination of BAP + NAA, BAP + IAA, BAP + IBA, and IAA + IBA at different concentrations with charcoal supplementation was studied. The result revealed that the use of different growth regulators had significant effect on different parameters studied. The maximum weight of PLBs (5.123 g) was obtained from the combination of BAP + IBA at 1.0 mg/l each. The highest shoot height (3.239 cm) and maximum number of rooted plantlets (4.473) was obtained from 1.0 mg/l each of BAP + NAA combination. The maximum number of leaves (3.490) and the maximum length of leaves (1.946 cm) were obtained from 1.0 mg/l each of BAP + IBA and the highest leaf width (1.166 cm) was obtained from 0.5 mg/l BAP +1.0 mg/l IBA combination. The highest root length was obtained from 0.5 mg/l each of BAP + IAA and the maximum number of regenerated plantlets (20) was obtained from 0.5 mg/l IAA + 1.0 mg/l IBA combination. However, the maximum fresh weight of single shoot (0.219 g) and the maximum number of roots per plantlet (6.300) was obtained from 1.0 mg/I each of IAA + IBA combination. Keywords: Dedrobium; orchid; hybrid; In vitro growth. DOI: 10.3329/bjar.v35i3.6457Bangladesh J. Agril. Res. 35(3) : 507-514

scholarly journals PENGARUH NAA DAN BAP TERHADAP PERTUMBUHAN SUBKULTUR ANGGREK HASIL PERSILANGAN Dendrobium biggibum X Dendrobium liniale

2016 ◽  
Vol 31 (1) ◽  
pp. 33
Author(s):  
Sri Hartati ◽  
Agus Budiyono ◽  
Ongko Cahyono
Keyword(s):  
Plant Conservation ◽  
Naphthalene Acetic Acid ◽  
Randomized Design ◽  
Significant Difference ◽  
Two Factors ◽  
Number Of Leaves ◽  
Media Research ◽  
Test Level ◽  
Range Test

<p><em>Indonesia has a high diversity of species of orchids. One of them is the Dendrobium orchid. Orchid seeds do not have endosperm should be grown in a medium that has enough nutrients. Orchid propagation in vitro is strongly influenced by the composition of the medium used. Research through experiments aimed at studying the effect of NAA, BAP, NAA and BAP combination of the subculture plantlets growth of  </em><em>hybrids</em><em> orchid Dendrobium biggibum X liniale in Vacin Went media. Research conducted at the Laboratory of Tissue Culture Center for Plant Conservation Bogor Botanical Gardens. </em><em>The experimental design used was completely randomized design (CRD) with two factors. The first factor was the concentration of Naphthalene Acetic Acid /NAA ( 0 ppm, 1 ppm, 3 ppm and 5 ppm). The second factor  was the type media of </em><em>Benzyl Amino Purine</em><em> /BAP ( 0 ppm, 1 ppm, 3 ppm and 5 ppm). </em><em>Each treatment was replicated eight times. Analysis of the data by F test level 5% and if there is a significant difference continued with Duncan Multiple (Duncan's Multiple Range Test) level of 5%. The result showed that </em><em>the addition  </em><em>of NAA 3 ppm </em><em>as much as 4,96 cm </em><em> and BAP 3 ppm </em><em>as much as 4,41 cm </em><em> give a significant effect on the increase of high plantlets</em><em> </em><em>and  so NAA 3 ppm </em><em>as much as 5,76 cm </em><em> effect  on  roots length , but did not significantly effect the number of leaves and roots.</em></p>

scholarly journals The in vitro shoot regeneration of Ehretia asperula Zol. and Mor.

2019 ◽  
Vol 2 (6) ◽  
pp. 75-83
Author(s):  
Le Thi Thuy Tien
Keyword(s):  
Woody Plant ◽  
Ms Medium ◽  
Benzyl Adenine ◽  
Shoot Initiation ◽  
In Vitro Shoots ◽  
Woody Plant Medium ◽  
Number Of Leaves ◽  
Growth Experiments ◽  
In Vitro Shoot Regeneration

Xa den young branches in the orchard were sterilized and used as explants for shoot initiation and growth experiments. The shoot induction was carried out with BA (benzyl adenine) or TDZ (thidiazuron). New shoots sprouted after one week of culture and the highest shoots (2.02 cm) were on MS medium (Murashige and Skoog medium) with BA 0.6 mg/L after 3 weeks. Furthermore, the number of leaves per shoot was also higher than other treatments (8.31 leaves per shoot). In vitro shoots were used in other experiments to investigate the effects of explants, biotin concentrations, minerals, type and concentration of cytokinins on the formation and elongation of shoots and clusters. When 5 mg/L biotin was added to MS medium, shoots grew better. The MS medium appeared to be most suitable for the initiation and elongating of shoots, followed by WPM (woody plant medium) and SH medium (Schenk and Hildebrandt medium), while B5 medium (Gamborg B5 medium) was the least effective. The spouting from three-week-old explants was earlier than others (4 and 5 weeks of age), which in turn affected on the shoot elongating. BA 1.5 mg/L was suitable to induce shoot clusters (3.91 shoots per explant) after 4 weeks.

scholarly journals Salinity-Induced Alterations in the Growth, Membranes and Osmolytes of Musa spp. cv. Caipira (AAA) During Micropropagation

2019 ◽  
Vol 11 (6) ◽  
pp. 171
Author(s):  
Yuri Lima Melo ◽  
Isabele Aragão Gomes Trindade ◽  
Monique Cristina Simão Lopes ◽  
Cibelley Vanúcia Santana Dantas ◽  
Josemir Moura Maia ◽  
...  
Keyword(s):  
Survival Rate ◽  
Plant Height ◽  
Exposure Time ◽  
Root Length ◽  
Growth Traits ◽  
In Vitro Rooting ◽  
Ms Medium ◽  
Musa Spp ◽  
Number Of Leaves

The aim of this study was to determine the concentration and exposure time to NaCl suitable for the micropropagation of banana, through the analysis of growth traits. Banana propagules were inoculated in MS medium with different concentrations of NaCl (0; 50; 75 and 100 mM) for 120 days (multiplication and rooting, 60 days each), with monthly subcultures. These propagules were measured for plant height, number of leaves, sprouting rate, average number of formed propagules, rooting rate, root length and survival rate. After 30 days, NaCl reduced sprouting rate at multiplication; the number of leaves, rooting rate and root length in rooting; and the height and propagules number in both phases. After 60 days, the NaCl affected the sprouting rate and propagules number in the multiplication; length of root in rooting; and the height and number of leaves in both phases. After 120 days, the reduction in the survival rate was proportional to the increase of NaCl in the medium. Thus, it is concluded that NaCl reduces most of the growth traits and the treatments with 75 and 100 mM NaCl affected multiplication and in vitro rooting more intensely.

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