Isolation and Characterization of Vanillin from Coconut Husk Lignin via Alkaline Nitrobenzene Oxidation

2014 ◽  
Vol 67 (4) ◽  
Author(s):  
Norzita Ngadi ◽  
Noor Amirah Abdul Halim ◽  
Mohammad Nasir Mohamad Ibrahim
Keyword(s):  
Gas Chromatography ◽  
Infrared Spectroscopy ◽  
Crystallization Process ◽  
Black Liquor ◽  
Coconut Husk ◽  
Isolation And Characterization ◽  
Ftir Characterization ◽  
Isolated Compound ◽  
Detection And Quantification

Isolation of vanillin from lignin extracted from coconut husk is presented in this study. Phosphoric acid (20%) is employed to precipitate the lignin from the black liquor produced by cooking the mixture of coconut husk and 1M NaOH in steam autoclave at 121ºC for one hour. Separation of vanillin from lignin was carried out via oxidation by adding 0.1 g of coconut husk lignin into a mixture of 2M NaOH (10 ml) and nitrobenzene (0.4 ml) in steel capillary bomb heated at 160ºC for 2.5 hours. Detection and quantification of vanillin presents in the lignin oxidized mixture was performed by liquid and gas chromatography. The recovery of vanillin from the mixture was done via crystallization process by dissolving the mixture in acetone and heats up to 60ºC with periodic stirring. Vanillin crystal appeared by rapidly cooled the heated mixture in the ice bath. The vanillin obtained was characterized by Infrared Spectroscopy (FTIR) for component verification. Component isolation by HPLC and GC-FID denoted the retention time of vanillin appeared around 18.852 minutes and 16.913 minutes respectively. A yield of 2.83 % vanillin was isolated from 0.1 g lignin sample and the result of FTIR characterization has suggested that the isolated compound is vanillin.

scholarly journals Isolation and Characterization of Hypolipidemic Compound from Cajanus cajan

2011 ◽  
Vol 24 (1) ◽  
pp. 6-10
Author(s):  
M Anwar Habib ◽  
ASM Anisuzzaman ◽  
RK Barman ◽  
M Abdul Gafur ◽  
M Tofazzal Haque
Keyword(s):  
Cajanus Cajan ◽  
Methanol Extract ◽  
Fasting Blood Glucose ◽  
Hypolipidemic Activity ◽  
1H And 13C Nmr ◽  
Isolation And Characterization ◽  
Nmr Data ◽  
Biological Studies ◽  
Isolated Compound

The study was carried out to identify the compound responsible for hypolipidemic and hypoglycemic effects of Cajanus cajan (redgram). The methanol extract of redgram seeds was found to decrease fasting blood glucose and lipid profile (p<0.001) on streptozotocin-induced mice compared to control. This activity- guided fraction led to the isolation of a compound, substituted benzene containing polyhydroxy functions fused with lactone (CCA3) by analysis of 1H and 13C-NMR data. Biological studies of the isolated compound possessed prominent hypolipidemic activity. Although a number of hypoglycemic compounds are reported, yet not any hypolipidemic compound from redgram. The compound CCA3 seems to be the first report on hypolipidemic activity from methanol extract of redgram. TAJ 2011; 24(1): 6-10

ISOLATION AND CHARACTERIZATION OF ARISTOLOCHIC ACID - I FROM ARISTOLOCHIA INDICA LINN. LEAVES

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (09) ◽  
pp. 52-53
Author(s):  
M.B Mulik ◽  
◽  
K.S. Laddha
Keyword(s):  
Aristolochic Acid ◽  
Chloroform Extract ◽  
Isolation Method ◽  
Aristolochic Acids ◽  
Isolation And Characterization ◽  
Isolated Compound ◽  
Anti Fungal ◽  
Aristolochic Acid I ◽  
Aristolochia Species

Aristolochic Acids (AAs) are major components of plants in Aristolochia species. The plant Aristolochia indica Linn. has diverse biological actions such as analgesic, anti-diabetics, anti-bacterial, anti-fungal and treatment of malaria and fevers. Here, we report a new and simple isolation method of Aristolochic Acid-Ι (AA-I) from leaves of Aristolochia indica Linn. Defatted leaves of A. indica were extracted with chloroform by soxhlation. AA-I was further isolated from the chloroform extract by partitioning with aqueous sodium bicarbonate. AA-I was purified by repetitive washings with methanol and recrystallization in methanol yielded yellow leaflets. The purity of the isolated compound was ascertained by HPLC analysis. structural elucidation of the isolated compound was done by IR, MS and NMR spectral analysis.

Isolation and characterization of sodium 2-fluoroacetate from Mascagnia rigida using chromatography and infrared spectroscopy

Toxicon ◽  
2012 ◽  
Vol 60 (3) ◽  
pp. 329-332 ◽  
Author(s):  
Luciana Castro da Cunha ◽  
Fernando Pípole ◽  
Luciana Retz de Carvalho ◽  
João Henrique Ghilardi Lago ◽  
Silvana Lima Górniak
Keyword(s):  
Infrared Spectroscopy ◽  
Isolation And Characterization

scholarly journals Isolation, characterization and biological properties of betulin from Entada africana Guill. and Perr. (Mimosaceae).

2018 ◽  
Vol 1 (2) ◽  
Author(s):  
A. Kwaji ◽  
H. M. Adamu ◽  
I. Y. Chindo ◽  
R. Atiko
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Gradient Elution ◽  
Stem Bark ◽  
Salmonella Typhi ◽  
Biological Properties ◽  
Pure Compound ◽  
Isolation And Characterization ◽  
Isolated Compound

The present study is aimed at the isolation and characterization of betulin from Entada africana.  A dichloromethane soluble portion of the stem bark methanol/acetone (1:1 v/v) extract was subjected to gradient elution using ethyl acetate in hexane (5 – 30 %) on an open column. A pure compound was obtained with Rf = 0.61 in hexane/ethyl acetate (8:2 v/v) after repeated washing and recrystallization from methanol and coded Enac1. The pure compound was analyzed using IR, 1H & 13C NMR and GC-MS. Clinical isolates of Escherichia coli, Klebsiella pneumoniae, Salmonella typhi and Staphylococcus aureus were used to assess the antibacterial activity of the pure compound while its preliminary Cytotoxicity was evaluated using brine shrimp nauplii. Based on the spectroscopic data obtained and in comparison with literature, the isolated compound was identified as betulin. The minimum inhibitory concentration (MIC) of betulin ranged between 62.50 - 250.00 µg/mL for all the four bacterial isolates in the study while its fifty percent lethal concentration (LC50) was 10.00 µg/mL. Significant Cytotoxicity with moderate antibacterial activity was observed. The study therefore justifies the existence of bioactive compounds in the stem bark of Entada africana and its use in traditional medicine.

Isolation and characterization of glycerides in human hair lipids by thin-layer and gas chromatography

Lipids ◽  
1966 ◽  
Vol 1 (4) ◽  
pp. 274-278 ◽  
Author(s):  
Eric J. Singh ◽  
Leon L. Gershbein ◽  
Hugh J. O'Neill
Keyword(s):  
Gas Chromatography ◽  
Thin Layer ◽  
Human Hair ◽  
Isolation And Characterization

Preparation, isolation, and characterization of permethylated galactosides and fucosides

1999 ◽  
Vol 77 (3) ◽  
pp. 319-325 ◽  
Author(s):  
Daniel D Asres ◽  
Hélène Perreault
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
Gas Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Characterization Methods ◽  
Isolation And Characterization ◽  
Nmr Data ◽  
Layer Chromatography ◽  
Structural Aspects

Sugar analysis involving permethylation followed by methanolysis can lead to significant results, given that permethylated monosaccharide standards are available for comparison of spectral and chromatographic data. Such standards are not readily commercially available, especially the furanoside forms. This note describes the isolation and characterization of permethylated pyranoside and furanoside species of D(+)-galactose and L(-)-fucose. Separation of the isomers was optimized using a combination of column chromatography and continuous elution thin-layer chromatography (TLC). TLC, gas chromatography - mass spectrometry, and 1H nuclear magnetic resonance (NMR) spectroscopy were used as the characterization methods. The isolation of furanosides is emphasized, since no specific NMR data have been reported on those to date, while several reports have already discussed the structural aspects of pyranosides.Key words: permethylation, monosaccharide, GC-MS, TLC, NMR.

Sign in / Sign up

Export Citation Format

Share Document