ESTIMATION OF BIOACTIVE PHENOLIC COMPOUNDS OF ABIES PINDROW AERIAL PARTS USING TLC DENSITOMETRY

INDIAN DRUGS ◽  
2017 ◽  
Vol 54 (04) ◽  
pp. 32-37
Author(s):  
D. Kumar ◽  
S. Kumar ◽  
Keyword(s):  
Phenolic Compounds ◽  
Benzoic Acid ◽  
Gallic Acid ◽  
Protocatechuic Acid ◽  
Coumaric Acid ◽  
Aerial Parts ◽  
Ich Guidelines ◽  
Validation Parameters ◽  
Abies Pindrow ◽  
Solvent Systems

The present investigation was undertaken to estimate contents of bioactive phenolic compounds in A. pindrow aerial parts using TLC densitometry. Maltol, p-coumaric acid, p-hydroxy benzoic acid, protocatechuic acid and gallic acid were resolved in TLC of methanol extract of plant using solvent systems – chloroform:methanol (9.7:0.3; scanned at 276 nm), toluene:diethyl ether (1:1; scanned at 320 nm), chloroform:methanol:glacial acetic acid (19:1:1; scanned at 266 nm), chloroform:methanol:formic acid (9:1:1; scanned at 295 nm) and chloroform:methanol:acetonitrile: formic acid (12:3:3:2; scanned at 292), respectively. The developed methods for estimation of phenolic compounds in plant were validated as per ICH guidelines. The contents of maltol, p-coumaric acid, p-hydroxy benzoic acid, protocatechuic acid and gallic acid in plant were found to be 0.10280±0.00002, 0.00860±0.00000, 0.00780±0.00001, 0.10030±0.00003 and 0.06440±0.00002% w/w, respectively. On the basis of validation parameters, the developed TLC densitometric methods for estimation of phenolic compounds in the plant were found to precise, accurate and specific.

scholarly journals Comparison of phenolic profiles and antioxidant activity of three Ornithogalum L. species

2018 ◽  
Vol 44 (3) ◽  
pp. 299-306
Author(s):  
Gülin Renda ◽  
Arzu Özel ◽  
Emine Akyüz Turumtay ◽  
Burak Barut ◽  
Büşra Korkmaz ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Protocatechuic Acid ◽  
Radical Scavenging ◽  
Coumaric Acid ◽  
Antioxidant Power ◽  
Aerial Parts ◽  
Phenolic Profiles ◽  
Ferric Reducing Antioxidant Power ◽  
First Time

Abstract Background Aboveground parts and bulbs of Ornithogalum species are consumed as food and used in traditional medicine in worldwide. Objective It is aimed to report the antioxidant capacity and phenolic compounds content of Ornithogalum sigmoideum, Ornithogalum orthophyllum and Ornithogalum oligophyllum for the first time. Materials and methods Antioxidant activity of the crude methanol extracts of the aerial parts and the bulbs of the species were determined with 1,1-diphenyl-2-picryl-hydrazyl, superoxide radical scavenging, ferrous ion-chelating effect, phosphomolybdenum-reducing antioxidant power and ferric-reducing antioxidant power assay. The ethylacetate, diethylether and water subextracts from leaf and flower were analyzed to quantify selected phenolic compounds by HPLC-UV. Results Among the six extracts, the methanol extract of the aerial parts of O. orthophyllum contained the highest amount of phenolic compounds (GAE, 11.0 mg/g extract). The aerial parts of O. orthophyllum showed higher DPPH and SOD activities than the other extracts with the SC50 values of 0.39±0.05 mg/mL and 0.44±0.08 mg/mL, respectively. Protocatechuic acid, p-hydroxybenzoic acid, vanillic acid and p-coumaric acid were the most abundant compounds at all the subextracts. Conclusion The antioxidant activity is found to be in accordance with the levels of phenolic content in the extracts.

scholarly journals The effects of plant density and irrigation on phenolic content in cauliflower 

2017 ◽  
Vol 44 (No. 4) ◽  
pp. 178-185 ◽  
Author(s):  
Alina Kałużewicz ◽  
Jolanta Lisiecka ◽  
Monika Gąsecka ◽  
Włodzimierz Krzesiński ◽  
Tomasz Spiżewski ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Ferulic Acid ◽  
Gallic Acid ◽  
Caffeic Acid ◽  
Phenolic Acids ◽  
Plant Density ◽  
Phenolic Content ◽  
Sinapic Acid ◽  
Dry Weight ◽  
Coumaric Acid

This study was conducted to study the influence of plant density and irrigation on the content of phenolic compounds, i.e., phenolic acids and flavonols in cv. ‘Sevilla’ cauliflower curds. Levels of phenolic acids and flavonols were in the range of 3.0–6.2 mg and 25.4–87.8 mg/100 g of dry weight, respectively, depending on plant density and irrigation. Of the phenolic acids, caffeic acid was detected in the highest amount, followed by p-coumaric acid, sinapic acid, gallic acid, and ferulic acid. Of the two flavonols detected, the levels of quercetin were higher than those of kaempferol. The content of the detected phenolic acids (with the exception of ferulic acid) and both flavonols increased with increasing plant density. Furthermore, the concentration of phenolic compounds (with the exception of ferulic acid) was significantly higher under irrigation.

scholarly journals A New Noriridoid and Six Phenolic Compounds from Rhopalocnemis phalloides

2018 ◽  
Vol 13 (12) ◽  
pp. 1934578X1801301 ◽  
Author(s):  
Nguyen Quang Hung ◽  
Nguyen Thi Luyen ◽  
Nguyen The Cuong ◽  
Tran Huy Thai ◽  
Nguyen Thanh Tung ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Phenolic Compounds ◽  
Gallic Acid ◽  
Protocatechuic Acid ◽  
Inhibitory Effect ◽  
Spectroscopic Methods ◽  
Hydroxybenzoic Acid ◽  
Nitric Oxide Production ◽  
Oxide Production ◽  
The Family

A rare noriridoid and six known phenolic compounds were isolated from the parasite plant Rhopalocnemis phalloides. Using spectroscopic methods, these compounds were identified as 10-acetoxy- cis-2-oxabicyclo[4.3.0]nonan-7-en-3-one (1), p-hydroxybenzoic acid (2), protocatechuic acid (3), gallic acid (4), coniferyl aldehyde (5), l- O-trans-cinnamoyl-β-D-glucoside (6), and coniferin (7). The noriridoid compound is the first reported from the family Balanophoraceae. Of the isolated compounds, coniferyl aldehyde had the strongest inhibitory effect on nitric oxide production (IC50 = 8.24 μM).

Biosynthese von p-Hydroxybenzoesäure und anderer Benzoesäuren in höheren Pflanzen

1964 ◽  
Vol 19 (5) ◽  
pp. 398-405 ◽  
Author(s):  
M. H. Zenk ◽  
G. Müller
Keyword(s):  
Benzoic Acid ◽  
Protocatechuic Acid ◽  
Higher Plants ◽  
Side Chain ◽  
Hydroxybenzoic Acid ◽  
Benzoic Acids ◽  
Coumaric Acid ◽  
Cinnamic Acids ◽  
Feeding Experiments ◽  
Catalpa Ovata

Feeding experiments with glucose- (2-14C), phenylalanine- (3-14C), tyrosine- (3-14C) and p-coumaric acid- (3-14C) showed that the latter three substances are incorporated in good yields into p-hydroxybenzoic acid in leaves of Catalpa ovata. Kinetic experiments showed that p-hydroxybenzoic acid is formed from phenylalanine via p-coumaric acid and the subsequent β-oxidation of the side chain. p-Hydroxybenzoic acid can also be synthetised by hydroxylation of benzoic acid, but this does not seem to be the biosynthetic route in Catalpa.Phenylalanine- (3-14C) is also incorporated into benzoic acid, protocatechuic acid, and vanillic acid by different plants; the radioactivity of the β-C atom of the amino acid was found in each case to be located in the carboxyl group of the C6 — C1 acid. This suggests that in higher plants the benzoic acids are formed from the corresponding cinnamic acids via β-oxidation.

Antioxidant Activity and Phenolic Compound Profile of Pistachio Skins (Pistacia vera L., Cultivars Kallehghuchi and Ohadi)

2020 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Shima Azadedel ◽  
Parichehr Hanachi ◽  
Azra Saboora
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Benzoic Acid ◽  
Gallic Acid ◽  
Cinnamic Acid ◽  
High Mass ◽  
Total Phenolic ◽  
Pistacia Vera ◽  
Native Plant ◽  
Pharmaceutical Industries

Background: Pistachio, with the scientific name Pistacia vera L., a native plant in Asia, is a member of the Anacardiaceae family. Pistachio nuts and skins are known as a rich source of phenolic compounds with antioxidant, anti-inflammatory, and antimicrobial properties. Objectives: In the current study, the industrial production of phenolic compounds with antioxidant activity was investigated because of the high mass production of pistachio skin in Iran. Methods: The extraction of two pistachio cultivars, namely Kalleghuchi and Ohadi were carried out by using two methods (maceration and ultrasonic extraction) and four solvents (acetone 70%, ethanol 50%, methanol 50%, and water). Antioxidant properties of pistachio skins were determined by three methods (Folin-Ciocalteau colorimetric method, DPPH assay, TLC/DPPH analysis). Results: The results showed that the highest content of total phenolic compounds were measured by ultrasonic and maceration methods related to Ohadi and Kallehghuchi in acetone solvents 17.4 ± 0.04 and 17.26 ± 0.1 mg/g DW, respectively. The highest antioxidant activity was measured by ultrasonic and maceration methods related to Ohadi in acetone and water solvents IC50 = 0.057 ± 0.001 and 0.059 ± 0.002 µg/ml, respectively. By TLC/DPPH analysis, gallic acid, 4-hydroxy-3, 5-dimethoxy benzoic acid, tannic acid, and some unidentified compounds were determined. By HPLC analysis, gallic acid, coumaric acid, cinnamic acid, 4-hydroxy-cinnamic acid, and 4-hydroxy benzoic acid were determined. Conclusions: In conclusion, this study clarifies some special biochemical characteristics of pistachio skins. Therefore, according to the results of the study, pistachio skins could be successfully used in the food and pharmaceutical industries.

scholarly journals Anti-malarial Activity Of Phenolic Acids Is Structurally Related

2020 ◽  
Author(s):  
Prince A Fordjour ◽  
Jonathan P Adjimani ◽  
Bright Asare ◽  
Nancy O Duah-Quashie ◽  
Neils B Quashie
Keyword(s):  
Plasmodium Falciparum ◽  
Ferulic Acid ◽  
Chlorogenic Acid ◽  
Gallic Acid ◽  
Caffeic Acid ◽  
Phenolic Acids ◽  
Rosmarinic Acid ◽  
Protocatechuic Acid ◽  
Antimalarial Activity ◽  
Coumaric Acid

Abstract Background In the absence of an effective vaccine against malaria, chemotherapy remains a major option in the control of the disease. Then, the recent report of the emergence and spread of clones of Plasmodium falciparum resistant to available antimalarial drugs should be of concern as it poses a threat to disease control. Compounds whose pharmacological properties have been determined and touted for other disease can be investigated for antimalarial activity. Phenolic acids (polyphenols) have been reported to exhibit antioxidant, anticancer, anti-inflammatory, antiviral and antibiotic effects. However, information on their antimalarial activity is scanty. Phenolic acids are present in a variety of plant-based foods: mostly high in the skins and seeds of fruits as well as the leaves of vegetables. Systematic assessment of these compounds for antimalarial activity is therefore needed. Method Using the classical in vitro drug test, the antimalarial activities of five hydroxycinnamic acids, (caffeic acid, rosmarinic acid, chlorogenic acid, o-Coumaric acid and ferulic acid) and two hydroxybenzoic acids (gallic acid and protocatechuic acid) against 3D7 clones of Plasmodium falciparum was determined. Results Among the phenolic acids tested, caffeic acid and gallic acid were found to be the most effective, with mean IC 50 value of 17.73µg/ml and 26.59µg/ml respectively for three independent determinations. Protocatechuic acid had an IC 50 value of 30.08 µg/ml. Rosmarinic acid and chlorogenic acid, showed moderate antimalarial activities with IC 50 values of 103.59µg/ml and 105µg/ml respectively. The IC 50 values determined for ferulic acid and o-Coumaric acid were 93.36µg/ml and 82.23µg/ml respectively. Conclusion The outcome of this study suggest that natural occurring phenolic compounds have appreciable level of antimalarial activity which can be exploited for use through combination of actions/efforts including structural manipulation to attain an increase in their antimalarial effect. Eating of natural food products rich in these compounds could provide antimalarial prophylactic effect.

scholarly journals Profiling of the Major Phenolic Compounds and Their Biosynthesis Genes in Sophora flavescens Aiton

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Jeongyeo Lee ◽  
Jaeeun Jung ◽  
Seung-Hyun Son ◽  
Hyun-Bi Kim ◽  
Young-Hee Noh ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Phenolic Acid ◽  
Hplc Analysis ◽  
Developmental Stages ◽  
Biological Activities ◽  
Expression Patterns ◽  
Coumaric Acid ◽  
Sophora Flavescens ◽  
Catechin Hydrate ◽  
Aerial Parts

Sophorae Radix (Sophora flavescens Aiton) has long been used in traditional medicine in East Asia due to the various biological activities of its secondary metabolites. Endogenous contents of phenolic compounds (phenolic acid, flavonol, and isoflavone) and the main bioactive compounds of Sophorae Radix were analyzed based on the qualitative HPLC analysis and evaluated in different organs and at different developmental stages. In total, 11 compounds were detected, and the composition of the roots and aerial parts (leaves, stems, and flowers) was significantly different. trans-Cinnamic acid and p-coumaric acid were observed only in the aerial parts. Large amounts of rutin and maackiain were detected in the roots. Four phenolic acid compounds (benzoic acid, caffeic acid, ferulic acid, and chlorogenic acid) and four flavonol compounds (kaempferol, catechin hydrate, epicatechin, and rutin) were higher in aerial parts than in roots. To identify putative genes involved in phenolic compounds biosynthesis, a total of 41 transcripts were investigated. Expression patterns of these selected genes, as well as the multiple isoforms for the genes, varied by organ and developmental stage, implying that they are involved in the biosynthesis of various phenolic compounds both spatially and temporally.

scholarly journals Phytochemicals of Conocarpus spp. as a Natural and Safe Source of Phenolic Compounds and Antioxidants

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 1069
Author(s):  
Hanan S. Afifi ◽  
Hassan M. Al Marzooqi ◽  
Mohammad J. Tabbaa ◽  
Ahmed A. Arran
Keyword(s):  
Phenolic Compounds ◽  
Protocatechuic Acid ◽  
Ethanol Concentration ◽  
Sinapic Acid ◽  
Food Additive ◽  
Natural Food ◽  
Coumaric Acid ◽  
Plant Parts ◽  
Conocarpus Erectus ◽  
Different Parts

Optimization of the extraction conditions of polyphenolic compounds for different parts of the Damas species, Conocarpus lancifolius and Conocarpus erectus, grown under UAE conditions was studied. The combination of ethanol concentration (50, 75, and 100%), temperature (45, 55, and 65 °C) and time (1, 2, and 3 h) was used by applying the Response Surface Methodology. The data showed that the extracts (n = 90) contained phenolic compounds, flavonoids, and tannins, and were free of alkaloids. Changing the extraction conditions had a significant effect on the detection of phytosterols, saponins, and glycosides and on the solubility of vanillic acid, p-coumaric acid, sinapic acid, t-ferulic acid, rutin hydrate, protocatechuic acid, quercetin, and flavone. The data reveal that the roots and leaves of C. erectus and the leaves and fruits of C.lancifolius are the most important plant parts from which to extract these compounds. This study draws attention to the unordinary use of Conocarpus spp. as a source of natural food additive.

scholarly journals PHENOLIC COMPOUNDS FROM CALLISTEMON CITRINUS LEAVES AND STEMS

2016 ◽  
Vol 54 (2) ◽  
pp. 190 ◽  
Author(s):  
Pham Ngoc Khanh ◽  
Ho Viet Đuc ◽  
Tran Thu Huong ◽  
Vu Thi Ha ◽  
Doan Thi Van ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Benzoic Acid ◽  
Protocatechuic Acid ◽  
2D Nmr ◽  
Chemical Investigation ◽  
Phytochemical Analysis ◽  
Methyl Gallate ◽  
Bioactive Constituents ◽  
Esi Ms ◽  
Benzoic Acid Derivatives

In the search for bioactive constituents from Vietnamese plants,  the leaves and stems of Callistemon citrinus (Curtis) Skeels were selected for chemical investigation. Phytochemical analysis of plant led to the isolation of eight phenolic compounds including two flavonoids (eucalyptine (1) and 8-demethyleucalyptine (2)), two alcohols (blumenol A (3), n-tetratriacontanol (4)), three benzoic acid derivatives (acid gallic (5), methyl gallate (6) protocatechuic acid (7)), one sterol (b-sitosterol (8)), and along with one sesquiterpene (2,6,10-bisabolatriene (9)). The structures of the natural compounds were determined by spectroscopic evidences including 1D- and 2D-NMR and ESI-MS.

Effects of Salinity Stress on Growth and Phenolics of Rice (Oryza sativa L.)

2016 ◽  
Vol 57 ◽  
pp. 1-10 ◽  
Author(s):  
Luong The Minh ◽  
Do Tan Khang ◽  
Pham Thi Thu Ha ◽  
Phung Thi Tuyen ◽  
Truong Ngoc Minh ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Ferulic Acid ◽  
Salinity Stress ◽  
Protocatechuic Acid ◽  
Total Phenolics ◽  
Susceptible Cultivar ◽  
Coumaric Acid ◽  
Rice Varieties ◽  
Stress Changes ◽  
Chemical Contents

This study was conducted to determine the correlation between of salinity stress on growth and phenolic compounds in rice. It was observed that salinity stress caused a significant decrease in shoot lengths, fresh and dry weights of all rice varieties. Under salinity stress, changes of chemical contents also differed among phenolic compounds and rice cultivars. Total phenolics and flavonoids, and contents of vanillin and protocatechuic acid in tolerant varieties were strongly increased, whereas in contrast, they were markedly reduced in the susceptible cultivar. Ferulic acid and p-coumaric acid were detected only in tolerance rice. Vanillin and protocatechuic acid may play a role, but ferulic acid and p-coumaric acid may be much involved in the tolerant mechanism against salinity stress. Ferulic acid and p-coumaric acid and their derivatives are able to be exploited as promising agents to reduce detrimental effects of salinity stress on rice production.

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