GREEN SYNTHESIS OF SILVER NANOPARTICLES, METHOD DEVELOPMENT AND VALIDATION BY UV SPECTROSCOPY IN BULK PHARMACEUTICALS

INDIAN DRUGS ◽  
2021 ◽  
Vol 57 (10) ◽  
pp. 23-29
Author(s):  
Vipul Patel ◽  
Unnati Patel
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Method Development ◽  
Uv Spectroscopy ◽  
Spectroscopic Method ◽  
Colour Change ◽  
Toxic Waste ◽  
Ag Nps ◽  
Datura Metel ◽  
Validation Parameters

The silver nanoparticle is an antibacterial, nontoxic agent. Its conventional synthesis usually generates toxic waste while reaction proceeds hence, green synthesis approach is used have for synthesis of silver nanoparticles (Ag-NPs) by using Datura metel leaf extract as reducing agent. The colour change from colourless to brown was the visual identification for formation of Ag-NPs and showed absorption at 447 nm. The Ag-NPs were of size 180 nm analysed by SEM and characterised by FTIR, where carboxylic peak was obtained at 1569 cm-1, besides confirming presence of alcoholic group and polyphenols. Further, validated method was developed and linearity was found to be 0.995 and % RSD of validation parameters was found to be within the limit <2%. A simple, lab scale, rapid, accurate, precise and sensitive spectroscopic method has been developed and validated as per ICH guideline for synthesized Ag-NPs in bulk pharmaceuticals by UV vis spectrophotoscopy and it is applicable for routine analysis.

Green Synthesis of Silver Nanoparticles using Datura metel Flower Extract Assisted by Ultrasound Method and Its Antibacterial Activity

2021 ◽  
Vol 15 ◽  
Author(s):  
Is Fatimah ◽  
Habibi Hidayat ◽  
Bambang Nugroho ◽  
Saddam Husein
Keyword(s):  
Particle Size ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Ag Nps ◽  
Ultrasound Method ◽  
Flower Extract ◽  
Datura Metel ◽  
Uv Visible ◽  
Ultrasound Assisted

Background: Green synthesis method of nanoparticles has been developed for several years. Besides providing an environmental-friendly process, the green synthesis of nanoparticles using plant extract provides a synergistic effect of the secondary metabolite. In recent times, the study involving the intensification process in nanoparticle formation also attracts great attention. This research deals with the green synthesis of silver nanoparticles using Datura metel flower extract as an antibacterial agent. The use of an ultrasound-assisted method for the synthesis is also investigated in this study. Methods: Synthesis of silver nanoparticles (AgNPs) using Datura metel flower extract under ultrasound-assisted method has been conducted. Evaluation of the successful synthesis was done using UV-visible spectrophotometry, particle size analyzer, x-ray diffraction, and transmission electron microscopy. The prepared AgNPs were tested as antibacterial against S. aureus, K. pneumoniae, S. pyogenes, and E. coli. Results: The ultrasound-assisted synthesis of AgNPs produces particles ranging from 25-70 nm in size; meanwhile, the reflux method demonstrated the size of 50-170 nm. These particle sizes represent the effect of the antibacterial activity as the ultrasound-assisted synthesized Ag NPs have a higher inhibition zone towards all tested bacteria. Subsequently, these data presented the applicability of Ag NPs synthesis using an ultrasound method as a potential candidate for biomedical applications. Conclusion: The profile of UV-Visible spectra and particle size analyses demonstrated the applicability of the ultrasound technique to produce a smaller size of the nanoparticles with higher antibacterial activity.

scholarly journals Single-Step Green Synthesis of Highly Concentrated and Stable Colloidal Dispersion of Core-Shell Silver Nanoparticles and Their Antimicrobial and Ultra-High Catalytic Properties

Nanomaterials ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 1007
Author(s):  
Azam Ali ◽  
Mariyam Sattar ◽  
Fiaz Hussain ◽  
Muhammad Humble Khalid Tareen ◽  
Jiri Militky ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Tannic Acid ◽  
Rate Constant ◽  
Catalytic Properties ◽  
Colloidal Dispersion ◽  
Alkaline Condition ◽  
Core Shell ◽  
Average Particle ◽  
Ag Nps

The versatile one-pot green synthesis of a highly concentrated and stable colloidal dispersion of silver nanoparticles (Ag NPs) was carried out using the self-assembled tannic acid without using any other hazardous chemicals. Tannic acid (Plant-based polyphenol) was used as a reducing and stabilizing agent for silver nitrate in a mild alkaline condition. The synthesized Ag NPs were characterized for their concentration, capping, size distribution, and shape. The experimental results confirmed the successful synthesis of nearly spherical and highly concentrated (2281 ppm) Ag NPs, capped with poly-tannic acid (Ag NPs-PTA). The average particle size of Ag NPs-PTA was found to be 9.90 ± 1.60 nm. The colloidal dispersion of synthesized nanoparticles was observed to be stable for more than 15 months in the ambient environment (25 °C, 65% relative humidity). The synthesized AgNPs-PTA showed an effective antimicrobial activity against Staphylococcus Aureus (ZOI 3.0 mM) and Escherichia coli (ZOI 3.5 mM). Ag NPs-PTA also exhibited enhanced catalytic properties. It reduces 4-nitrophenol into 4-aminophenol in the presence of NaBH4 with a normalized rate constant (Knor = K/m) of 615.04 mL·s−1·mg−1. For comparison, bare Ag NPs show catalytic activity with a normalized rate constant of 139.78 mL·s−1·mg−1. Furthermore, AgNPs-PTA were stable for more than 15 months under ambient conditions. The ultra-high catalytic and good antimicrobial properties can be attributed to the fine size and good aqueous stability of Ag NPs-PTA. The unique core-shell structure and ease of synthesis render the synthesized nanoparticles superior to others, with potential for large-scale applications, especially in the field of catalysis and medical.

scholarly journals Antibacterial activity of royal jelly-mediated green synthesized silver nanoparticles

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Susanna Gevorgyan ◽  
Robin Schubert ◽  
Mkrtich Yeranosyan ◽  
Lilit Gabrielyan ◽  
Armen Trchounian ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Royal Jelly ◽  
Fluorescence Emission ◽  
Spherical Particles ◽  
Ag Nps ◽  
Gram Negative ◽  
Green Synthesized Silver Nanoparticles ◽  
Higher Sensitivity

AbstractThe application of green synthesis in nanotechnology is growing day by day. It’s a safe and eco-friendly alternative to conventional methods. The current research aimed to study raw royal jelly’s potential in the green synthesis of silver nanoparticles and their antibacterial activity. Royal jelly served as a reducing and oxidizing agent in the green synthesis technology of colloidal silver nanoparticles. The UV–Vis maximum absorption at ~ 430 nm and fluorescence emission peaks at ~ 487 nm confirmed the presence of Ag NPs. Morphology and structural properties of Ag NPs and the effect of ultrasound studies revealed: (i) the formation of polydispersed and spherical particles with different sizes; (ii) size reduction and homogeneity increase by ultrasound treatment. Antibacterial activity of different concentrations of green synthesized Ag NPs has been assessed on Gram-negative S. typhimurium and Gram-positive S. aureus, revealing higher sensitivity on Gram-negative bacteria.

scholarly journals Glucuronoxylan-mediated silver nanoparticles: green synthesis, antimicrobial and wound healing applications

RSC Advances ◽  
2017 ◽  
Vol 7 (68) ◽  
pp. 42900-42908 ◽  
Author(s):  
Gulzar Muhammad ◽  
Muhammad Ajaz Hussain ◽  
Muhammad Amin ◽  
Syed Zajif Hussain ◽  
Irshad Hussain ◽  
...  
Keyword(s):  
Wound Healing ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Biological Applications ◽  
Ag Nps

Hydrogel forming polysaccharides are attracting attention for the design of diverse nature silver nanoparticles (Ag NPs) with potential biological applications.

Green Synthesis of Silver Nanoparticles Using Aqueous Floral Extract of Nelumbo Nucifera

2013 ◽  
Vol 756 ◽  
pp. 106-111 ◽  
Author(s):  
Selvaraj Arokiyaraj ◽  
Udaya Prakash Nyayiru Kannaian ◽  
Vijay Elakkya ◽  
T. Kamala ◽  
S. Bhuvaneswari ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Chemical Reduction ◽  
Colour Change ◽  
Cost Effective ◽  
Nelumbo Nucifera ◽  
Resonance Band ◽  
Afm Analysis ◽  
Reduction Methods ◽  
Physical And Chemical

The aim of the present study is to synthesize silver nanoparticles, using an aqueous floral extract of common Lotus, i.e Nelumbo nucifera. The synthesized nanoparticles were characterized using UV, TEM, EDX, AFM & XRD. The synthesized AgNPs were confirmed due to the colour change from colourless to reddish brown just after the addition of the aqueous floral extract of Nelumbo nucifera. The UV results of AgNPs showed the excitation of surface Plasmon resonance band at 427 nm. TEM results showed that the synthesized AgNPs were uniformed; monodispersed,spherical in shape and the particle size were found to be 77.81 ± 3.54 nm. EDX spectrum of AgNPs confirms strong signals from Ag (64%) and other elements such as C, O and Cl. The morphology of the synthesized AgNPs by AFM analysis resembled the TEM micrograph. The crystalline nature of the AgNPs was confirmed by XRD. The present study concludes that the aqueous floral extract of Nelumbo nucifera could be used as an effective reducing agent for the synthesis of AgNP. The green synthesis ofsilver nanoparticles is non-toxic and cost-effective and thus remains to be an alternative method to other physical and chemical reduction methods.

scholarly journals Green synthesis of bio-molecule encapsulated magnetic silver nanoparticles and their antibacterial activity

RSC Advances ◽  
2018 ◽  
Vol 8 (65) ◽  
pp. 37176-37183 ◽  
Author(s):  
A. K. M. Atique Ullah ◽  
M. F. Kabir ◽  
M. Akter ◽  
A. N. Tamanna ◽  
A. Hossain ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Pathogenic Bacteria ◽  
Ag Nps ◽  
Synthesis Technique

Persuaded by the necessity of finding new sources of antibiotics, Ag NPs were synthesized by adopting a newly developed green synthesis technique and subsequently, their antibacterial activity against different pathogenic bacteria was evaluated.

Green Synthesis of Silver Nanoparticles Using Waste Tea Leaves

2020 ◽  
Vol 3 (1) ◽  
pp. 1-14
Author(s):  
Darshana Rajput ◽  
Samrat Paul ◽  
Annika Gupta
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Dye Degradation ◽  
Uv Spectroscopy ◽  
Cost Effective ◽  
Cotton Cloth ◽  
Textile Dye ◽  
Phenol Red ◽  
Tea Leaves

Green synthesis of silver nanoparticles has gained momentum since the demand to synthesize nanoparticles in an eco-friendly way has increased significantly. Here we report, economic and cost-effective biosynthesis of silver nanoparticles using waste of tea leaves (Camelia sinesis). The aim of the study was to biosynthesize silver nanoparticles and to assess its potential applications such as antibacterial activity, plant growth induction and dye degradation. Standardization studies were done using UV- Spectroscopy to determine the optimum synthesis condition for synthesis of silver nanoparticles. The optimum conditions were found to be pH 6.0, ambient temperature condition and 5mM AgNO3 concentration. Characterization studies using UV-Visible Spectroscopy, TEM and AFM analysis show nanoscale range of the particles. The silver nanoparticles showed maximum antibacterial activity against K. pneumonia followed by E. coli and minimum activity against C. diptheriae. The nanoparticles showed significant effect on the growth of Vigna radiata seeds at 50% concentration of nanoparticles. The particles immobilized on cotton cloth showed antibacterial activity against Gram positive organisms. Dye degradation studies showed that the nanoparticles are able to degrade phenol red and blue textile dye effectively.

Preparation of silver nanoparticles using atmospheric discharge plasma for catalytic reduction of p-nitrophenol: the influence of pressure in the reactor

2020 ◽  
Vol 49 (6) ◽  
pp. 449-456 ◽  
Author(s):  
Margarita Skiba ◽  
Viktoria Vorobyova ◽  
Alexander Pivovarov ◽  
Inna Trus
Keyword(s):  
Silver Nanoparticles ◽  
Discharge Plasma ◽  
Uv Spectroscopy ◽  
Spherical Particles ◽  
Colloidal Solutions ◽  
Ag Nps ◽  
Sem Images ◽  
Temperature Plasma ◽  
Content Type ◽  
Atmospheric Discharge

Purpose This paper aims to synthesize silver nanoparticles using atmospheric discharge plasma in contact with liquid at different pressure in reactor and to assess their catalytical properties for reducing 4-nanoparticles (NP). Design/methodology/approach The Ag colloidal NPs was rapidly synthesized as a result of non-equilibrium low-temperature plasma formation between an electrode and the surface of AgNO3 solution for 5 min at different pressure in reactor. Synthesized Ag NPs were characterized with common analytical techniques. Ultraviolet–visible (UV) spectroscopy, dynamic light scattering, scanning microcopy analysis were used to study the formation and characteristics of silver nanoparticles. Findings The formation of silver colloidal solutions under plasma discharge at different pressure in reactor is characterized by the presence of surface resonance peak in the spectra. Scanning electron microscope (SEM) images confirmed the formation of spherical particles having a size distribution in the range of 15-26 nm. The AgNPs solution showed excellent rapid catalytic activity for the complete degradation of toxic 4-nitrophenol (4-NPh) into non-toxic 4-aminophenol (4-APh) within 18 min. Research limitations/implications Further studies are necessary for confirmation of the practical application, especially of deposition Ag NPs on TiO2. Practical implications The method provides a simple and practical solution to improving the synthesis of colloidal solutions of Ag NPs for degradation of organic pollutants (4-NPh) in water and wasters water. Originality/value Atmospheric discharge plasma in contact with liquid at different pressure can be used as an effective technique for synthesis of nanomaterials with catalytic properties.

scholarly journals Synthesis of Silver Nanoparticles Using Dichloromethane Extract of Chrysanthemum cinerariaefolium and Its Bioactivity

2021 ◽  
Vol 6 (1) ◽  
pp. 1-17
Author(s):  
Caroline Jepchirchir Kosgei ◽  
Meshack Amos Obonyo ◽  
Josphat Clement Matasyoh ◽  
James J. Owuor ◽  
Moses A. Ollengo ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Metallic Nanoparticles ◽  
Colour Change ◽  
Vero Cells ◽  
Ag Nps ◽  
Absorption Bands ◽  
Sem Images ◽  
Average Size ◽  
Chrysanthemum Cinerariaefolium

Common methods of synthesizing metallic nanoparticles are chemical and physical. However, they are expensive and use toxic chemicals. Green synthesis is less costly and safer hence a potential alternative. Silver nanoparticles (Ag NPs) were synthesized using dichloromethane extract of Chrysanthemum cinerariaefolium and colour change from pale green to dark brown was observed. Scanning Electron Microscopy (SEM) images were faceted and others formed clusters. Transmission Electron Microscopy (TEM) images were spherical with an average size of 22.8± 17.5 nm. EDX analysis showed the nanoparticles had percentage abundance of 67.26%. Fourier-transform Infrared Spectroscopy (FTIR) analysis showed absorption bands at 3489.59 cm-1, 3217.80 cm-1, 2384.74 cm-1 , 1633.05 cm-1, 1405.08 cm-1, 1109.32 cm-1 and 505.93 cm-1. The UV-Vis analysis showed Surface Plasmon Resonance (SPR) peak at 434 nm. The nanoparticles were more active on P. aeruginosa with an MIC of 15 µg/ml while the cytotoxicity assay showed Ag NPs had an MIC of 33.33 µg/ml hence were noncytotoxic against Vero cells.

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