scholarly journals Eucalyptus globulus Methanol Leaf Extract Improves Glycosylated Hemoglobin, Lipid Profile Levels and Haematological Sub-inflammatory Biomarkers in Streptozotocin-induced Diabetic Rats

2021 ◽  
Vol 1 (1) ◽  
Author(s):  
Ajilore B.S ◽  
Fatoki J.O ◽  
Abayomi T.A.
Keyword(s):  
Lipid Profile ◽  
Disease Progression ◽  
Eucalyptus Globulus ◽  
Leaf Extract ◽  
Standard Procedure ◽  
Glycosylated Hemoglobin ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Significance Level ◽  
Leaf Powder

Background: Eucalyptus leaf is used traditionally to treat a lot of diseases, but little is known about its use in the management of diabetes mellitus (DM) and its complications. Objectives: Antidiabetic property of Eucalyptus globulus leaf extract (EGLEX) was assessed on recent Makers of therapeutic response and diabetic disease progression in streptozotocin-induced diabetic rats. Methods: Eucalyptus globullus leaf powder (200 g) was extracted with methanol using standard procedure. Hyperglycemia was induced in Wistar rats with single intravenous dose of 50 mg/kg/bwt streptozotocin. The rats were divided into five groups (n = 5): Anormal control, B- diabetic control, C, D and E were diabetic rats treated with Eucalyptus globullus leaf extract (EGLEX), metformin and insulin respectively for four weeks. Samples were collected for biochemical and hematological studies. Data obtained were analysed using One Way Analysis of Variance at <0.05 significance level. Results and Conclusion: The results showed significant (p<0.05) blood glucose reduction of 68%, 51% and 68%, and weight gain of 17%, 18% and 11% in rats treated with EGLEX, metformin and insulin respectively. HBA1c level was significantly (p<0.05) raised (9.30%) in diabeticuntreated rats when compared with normal control (3.77%), EGLEX (4.24%), metformin (4.94%) and insulin (5.33%) groups. The deranged lipid profile indices, malondialdehyde and reduced glutathione levels, superoxide dismutase activity, platelet count, % neutrophil and % lymphocyte were normalized in diabetic rats treated with EGLEX when compared with the values in diabetic untreated rats. The findings concluded that EGLEX possesses antidiabetic property and improves biomarkers of diabetic disease progression.

scholarly journals Nephroprotective effect of ethanolic leaf extract of Thaumatococcus danielli (benth.) in streptozotocin induced diabetic Rats

2017 ◽  
Vol 7 (12) ◽  
pp. 923
Author(s):  
Olubukola Sinbad Olorunnisola ◽  
Adewale Adetutu ◽  
Rasaq B POPOOLA ◽  
Abiodun Olusoji Owoade ◽  
Peter Adegbola ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Standard Procedure ◽  
Folk Medicine ◽  
Inflammatory Cells ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Albino Rats ◽  
Nephroprotective Activity ◽  
Ethanolic Leaf Extract

Background: The leaves of Thaumatococcus danielli (Benth.) have been traditionally used in folk medicine to treat malaria in Nigeria. However, there is no report on whether these leaves contain Nephroprotective activity. Thus, the ethanol leaf extract was investigated for Nephron-protective activity in Streptozotocin induced diabetic rats.Methods: First, the LD50 of the leaf was determined using standard procedure. Rats were assigned to 5 groups (A-E) of five rats. Except for the control group, each group was made diabetic using Streptozotocin (65 mg/kg/b.wt. i p). The treated groups received 0.5 ml of glibenclamide (25mg/kg/b.wt, o.p), 250 and 500 mg/kg/b.wt, o.p of Thaumatococcus danielli respectively. After 14 days of treatment, animals were sacrificed under light anaesthesia. Data were expressed as Means ± S.D (n=5) and were analyzed using one-way ANOVA followed by Dunnet’s test, values were considered significant at p<0.05. Results: The plant showed a LD50 greater than 5000 mg/kg/b.wt in albino rats observed for 72 hours. A significant (p<0.05) decreased in serum Na+, Cl-, HCO-3, total protein, and an insignificant increase in K+, urea and creatinine level were observed in the diabetic group when compared with the normal group. Oral administration of plant extract and glibenclamide significantly (p<0.05) restored the electrolytes to near normal. Histological alterations such as glomerulonephritis, and tubules infiltration by inflammatory cells observed in diabetic control were also reversed.Conclusion: This study suggests renal protective ability of the plant against impairment due to hyperglycemia.Keywords: Streptozotocin, Thaumatococcus danielli, Nephroprotective, Glibenclamide, serum electrolytes.

scholarly journals Effect of Insulin Sensitizers and Sodium Glucose Cotransporter 2 Inhibitor on Glycemic State and Cardiovascular Performance in Type II Diabetic Rats

QJM ◽  
2020 ◽  
Vol 113 (Supplement_1) ◽  
Author(s):  
S F Ewida ◽  
A M H Shabaan ◽  
H F Eldomiaty ◽  
G S Y Hanna ◽  
S E Hassabelnabi ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Lipid Profile ◽  
Type Ii Diabetes ◽  
Glycosylated Hemoglobin ◽  
Diabetic Rats ◽  
Cardiac Performance ◽  
Aortic Tissue ◽  
Type Ii ◽  
Arterial Blood ◽  
Sodium Glucose Cotransporter

Abstract Background Cardiovascular disease is the most life-threatening diabetic complication. Type II diabetes may lead to damage of the heart muscle. Sodium glucose cotransporter 2 (SGL2) inhibitors are a new class of diabetic medications indicated only for the treatment of type II diabetes. Aim This investigation was assigned to compare the effect of metformin and SGL2 inhibitors (dapaglifilizone) in type II diabetic rats. Design and methods: Eighty rats divided into four groups were used: non diabetic; diabetic; diabetic metformin -treated; diabetic dapagliflizoline- treated. At the end, arterial blood pressure and cardiac performance (cardiac contractility and heart rate) were assessed. Serum glucose, glycosylated hemoglobin (HbA1c), insulin, lipid profile, total antioxidant capacity, malondialdehyde, tumor necrosis factor α were measured. HOMA-IR index was calculated. DNA changes were assessed from hearts and aortea. Aortic endothelial changes recorded using H&E and masson trichome techniques. Results Glycemic index, lipid profile, oxidative stress and inflammatory parameters were significantly improved in both metformin and dapagliflizoline treated groups with also significant improvement in blood pressure, Cardiac performance and reduction in collagen deposition in aortic tissue and DNA fragmentation. Dapagliflizoline treatment results were significantly improved in all parameters compared to metformin treatment. Conclusion SGL2 inhibitors (dapaglifilizone) successfully restored glycemic state, cardiac performance, DNA and endothelial changes in type II diabetic rats compared to metformin.

scholarly journals Antidiabetic potential of the leaf extract of Centella asiatica in alloxaninduced diabetic rats

2016 ◽  
Vol 4 (1) ◽  
pp. 51-59 ◽  
Author(s):  
Talha Bin Emran ◽  
Mycal Dutta ◽  
Mir Muhammad Nasir Uddin ◽  
Aninda Kumar Nath ◽  
Md Zia Uddin
Keyword(s):  
Body Weight ◽  
Leaf Extract ◽  
Total Cholesterol Level ◽  
Serum Insulin ◽  
Fasting Blood Glucose ◽  
Centella Asiatica ◽  
Liver Glycogen ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Diabetic Rat

The study was designed to evaluate the glucose and cholesterol lowering effect of the aqueous extract of Centella asiatica leaf using the alloxan-induced diabetic rats and compared the activity with diabetic control and antidiabetic drug (Glibenclamide). Leaf extract (50 mg/kg) of C. asiatica and Glibenclamide were administered to normal and experimental diabetic rats for the duration of 10 days. In the alloxan-induced diabetic rat model, C. asiatica extract (50 mg/kg) significantly (p < 0.05) lowered the fasting blood glucose level as well as the total cholesterol level. Serum insulin levels were not stimulated in the animals treated with the extract. In addition, changes in body weight, serum lipid profiles and liver glycogen levels assessed in the extract treated diabetic rats were compared with diabetic control and normal animals. Significant results (p < 0.05) were observed in the estimated parameters. Surprisingly, body weight was increased significantly (p < 0.05) in the C. asiatica treated diabetic group. Phytochemical screening showed the presence of alkaloids, flavonoids, glycosides, steroids and tannins in significant amountsJahangirnagar University J. Biol. Sci. 4(1): 51-59, 2015 (June)

scholarly journals Preclinical evaluation of antidiabetic activity of poly herbal plant extract in streptozotocin induced diabetic rats

2016 ◽  
Vol 5 (2) ◽  
pp. 45-49
Author(s):  
P.P. Gupta ◽  
◽  
J. Haider ◽  
R.P. Yadav ◽  
U. Pal ◽  
...  
Keyword(s):  
Body Weight ◽  
Plant Extract ◽  
Leaf Extract ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Sprague Dawley ◽  
Pancreatic Cell ◽  
Partial Restoration ◽  
Herbal Plant

Objective: To study and compare the effect of Poly herbal plant extract (PHPE) with Glibenclamide (GL) on various parameters in Streptozotocin (STZ) induced diabetic rats. Methods: Diabetes was induced by combining High Fat-diet and injecting low dose Streptozotocin (35 mg/kg body wt.) to Sprague-dawley rats. Diabetic rats were treated with chloroform leaf extract of Azadirachta indica, aqueous leaf extract of Bougainvillea spectabilis and ethanolic seed extract of Trigonella foenum graecum combined in ratio of 1:2:3 respectively at dose of 600 mg/kg body weight by oral gavaging for 28 days. The results were compared with standard anti diabetic drug Glibenclamide given in dose of 500 µg/kg body weight. Results: Increase in body weight of both PHPE and GL treated diabetic rats was found to be statistically significant (p<0.05) compared to diabetic control rats. Decline in FBG levels of both PHPE and GL treated diabetic rats were found to be highly significant statistically (p<0.001) when compared to diabetic control rats at the end of study. Total Cholesterol (TC) and Triglycerides level in diabetic rats treated with PHPE were found to be highly statistically significant (p<0.001) compared to diabetic control rats. Pancreas of PHPE treated diabetic rats revealed partial restoration in size and number of islet of langerhans. Reduction in widening between acinar and islet cells noted. Glibenclamide treated diabetic rats showed much more improvement in pancreatic cell architecture by returning to its normal structure and size. Conclusion: In present study PHPE has shown to decrease elevated FBG level and improve in body weight at the end of study in diabetic rats which can be suggested due to modification in carbohydrate metabolic pathways, stimulation of insulin production by the pancreas, increased peripheral utilization of glucose in the cells and regeneration of β-cells of the pancreas.

scholarly journals Assessment of Fertility Effect and Hypolipidemic Activity of Carica papaya (Linn) Leaf Methanol Extract in Male Diabetic Rats

2021 ◽  
pp. 58-69
Author(s):  
Opeyemi O. Ayodele ◽  
Ifeoluwa M. Dada ◽  
Rotimi K. Adekunle
Keyword(s):  
Lipid Profile ◽  
Plasma Glucose ◽  
Carica Papaya ◽  
Methanol Extract ◽  
Sperm Count ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Albino Rats ◽  
Spectrophotometric Methods

Aim: Diabetes mellitus (DM) is a prevalent metabolic disorder that leads to other microvascular and macrovascular complications. Diabetes affects fertility and blood clotting, and also cause impaired lipid profile thus leading to increased atherogenic risks and coronary diseases. This research investigates the effects of Carica papaya leaf methanol extract on fertility indices and lipid profile of male diabetic rats. Methodology: Male Wistar albino rats were randomly divided into five groups of six rats each. Diabetes was induced in the rats by a single intraperitoneal injection of streptozotocin (55 mg/kg). Diabetic rats were treated orally with 100 and 200 mg/kg C. papaya methanol extract for 14 days. At the end of administration, the plasma glucose concentration and lipid profile were assayed by spectrophotometric methods; seminal analysis was carried out for evaluation of morphology, motility and sperm count under the microscope. The bleeding and clotting times of the rats were also determined. Results: C. papaya leaf methanol extract caused significant (p = 0.05) reduction in plasma glucose, total cholesterol, triglycerides, VLDL-C, LDL-C, bleeding and clotting times of diabetic treated rats, while the HDL-C of treated groups were significantly (p = 0.05) elevated compared to the diabetic control. Percentage normal cells were lower in diabetic control rats (41.4±4.4%) and C. papaya treated groups (50.0±8.5% for 100 mg/kg; 47.5±9.1% for 200 mg/kg) compared with the normal control group (69.5±5.6%). Similar results were recorded for sperm count. The qualitative phytochemical screening showed the presence of steroids, anthraquinone, tannin, and other bioactive compounds. Conclusion: findings from this study indicated that C. papaya leaf methanol extract could possess hypoglycemic and hypolipidemic activities. Thus, could be considered as a potential source of bio pharmacological agent for management and control of DM and its complications. Prolonged administration of C. papaya leaves may negatively affect male fertility.

scholarly journals Lipid profile status of streptozotocin induced diabetic rats treated with ethanolic leaf extract of Solenostemon monostachyus

2015 ◽  
Vol 9 (8) ◽  
pp. 289-293 ◽  
Author(s):  
Amaechi Dennis ◽  
O. Udosen E. ◽  
E. Edet E. ◽  
E. Asanga E. ◽  
E. Mbakwe I.
Keyword(s):  
Lipid Profile ◽  
Leaf Extract ◽  
Diabetic Rats ◽  
Ethanolic Leaf Extract

scholarly journals An antioxidant activity of Cinnamonum tamala improves histopathological alterations and biochemical parameters in alloxan induced diabetic rats

2019 ◽  
Vol 10 (6) ◽  
pp. 50-56 ◽  
Author(s):  
Mamata Laxmikant Pochhi
Keyword(s):  
Serum Insulin ◽  
Glycosylated Hemoglobin ◽  
Antioxidant Properties ◽  
Insulin Level ◽  
Diabetic Control ◽  
Protective Role ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Standard Drug ◽  
Cinnamomum Tamala

Background: Diabetic mellitus is a multifactorial disorder associated with its devastating consequences has assumed epidemic proportion. Diabetes mellitus (DM) is a global health problem and the incidence of DM is increasing at alarming rate all over the world. Many Indian medicinal plants have been reported to possess potential antidiabetic activity and could play important role in the management of diabetes with less adverse effects. Aims and Objectives: The main objective of this study was to focus on the anti-diabetic activity of Cinnamomum tamala, with special reference to its curative and protective role in alloxan induced diabetic rats. Attempts were further made to study the antioxidant properties of C. tamala leaves. Materials and Methods: The diabetic rats were administered orally with the aqueous leaves extracts of Cinnamonum tamala (250 mg/kg) for 30 days. The results were compared with standard drug Tolbutamide. Result: The alloxan treated diabetic control rats showed a significant increase in the plasma glucose, glycosylated hemoglobin (HbA1C), glucose-6-phosphatase, aldolase, LDH, ALT, AST, ALP and GGT activity, freeradicals formation with a concomitant decrease in glycogen content in the liver and serum insulin level and phosphoglucoisomerase and hexokinase activity in tissues as compared to normal control rats. Oral administration of C. tamala extract for 30 days showed significant result as compared to Tolbutamide and diabetic control rats. Conclusion: On the basis of above findings it can be concluded that extracts of C. tamala to alloxan induced diabetic rats showed significant positive changes in the biochemical and histopathological parametersrelated to carbohydrate and protein metabolism.

scholarly journals Efficacy of a Methanolic Extract of Adansonia digitata Leaf in Alleviating Hyperglycemia, Hyperlipidemia, and Oxidative Stress of Diabetic Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Hossam Ebaid ◽  
Samir A. E. Bashandy ◽  
Ibrahim M. Alhazza ◽  
Iftekhar Hassan ◽  
Jameel Al-Tamimi
Keyword(s):  
Leaf Extract ◽  
Methanolic Extract ◽  
Glycosylated Hemoglobin ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Diabetic Rats ◽  
Diabetic Group ◽  
Dependent Manner ◽  
Adansonia Digitata ◽  
Necrosis Factor Alpha

Traditionally, in many countries, various parts of the Adansonia digitata (A. digitata) tree have been used in the treatment of many clinical ailments including diarrhea and dysentery. The phytochemical screening has indicated that the leaf extract of A. digitata contains flavonoids, saponins, mucilage, steroids, and alkaloids. Thus, this paper aims to evaluate the hyperglycaemic and hypolipidaemic effects of methanolic extract of A. digitata leaves (200 mg/kg and 400 mg/kg) in diabetic rats. The extract was administered orally for six weeks in the streptozotocin (STZ)-induced diabetic rats. The treatment with the extract caused a significant reduction in the blood glucose, glycosylated hemoglobin, cholesterol, triglycerides, low-density lipoprotein (LDL), interleukin 6 (IL-6), tumor necrosis factor-alpha (TNF-α), and malondialdehyde (MDA) levels by 46.7%, 46.15%, 48.91%, 43%, 60%, 66%, 45.45%, and 30.4%, respectively, as compared to the diabetic group after the sixth week of treatment. The leaf extract also mitigated the decline of high-density lipoprotein (HDL) level, RBCs count, hemoglobin level, packed cell volume (PCV %), and erythropoietin concentration in diabetic rats by 31%, 33.25%, 24.72%, 51.42%, and 220.68% with respect to the diabetic group. Also, the extract maintained the level of antioxidant enzymes, catalase (CAT) and superoxide dismutase (SOD), and reduced glutathione (GSH) in the diabetic rats. It also reduced the elevation in the white blood corpuscles (WBC) count in the STZ-induced diabetic rats. Our study, therefore, indicates that methanolic extract of A. digitata leaf exerts strong antidiabetic and hypolipidaemic properties in a dose-dependent manner by improving the hematological properties and redox parameters in the experimental diabetic rats.

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