scholarly journals Fatty acid profile as a tool to trace the origin of beef in pasture- and grain-fed young bulls of Retinta breed

2018 ◽  
Vol 15 (4) ◽  
pp. e0607 ◽  
Author(s):  
Alberto Horcada ◽  
Adoración López ◽  
Oliva Polvillo ◽  
Rafael Pino ◽  
Dolores Cubiles-de-la-Vega ◽  
...  

This research explores the possibility of using the fatty acid profiles of intramuscular fat to authenticate the origin of Retinta breed meat according to different feeding regimes based on the combined use of concentrate and grass or forage (GP, grass pasture; MC, medium concentrate; HC, high concentrate). Young bulls from GP (n=30) were reared on grass pasture and supplement with concentrate in controlled feeders; MC (n=30) and HC (n=15) were reared in farm buildings using 40 and 80% concentrate of total dry matter from diet, respectively. The stepclass function in R was used to perform a stepwise linear discriminant analysis including thirty fatty acids from intramuscular fat. Two fatty acids, 9c18:1 and 22:5 n-3 were selected as discriminators of the meat origin. Meat from the GP and MC was characterized by higher 22:5 n-3 (p<0.05), while HC meat showed higher 9c18:1 (p<0.05). The use of 9c18:1 and 22:5 n-3 fatty acids from intramuscular fat resulted in a correct assignation of 100% of beef samples to each of the feeding regimes. Therefore, in addition to serving as an effective tool for discriminating between feeding regimes in the origin of the beef, the fatty acid profile of intramuscular fat could help companies to check the authenticity of the meat origin.

2020 ◽  
Vol 50 (1) ◽  
pp. 47-54
Author(s):  
I De Gasperín ◽  
J.G. Vicente ◽  
J.M. Pinos-Rodríguez ◽  
F Montiel ◽  
R Loeza ◽  
...  

The aim of this research was to determine fatty acid profiles in piglet brain, skin, and muscle, and in the milk of sows fed fat with different saturation grades during gestation and lactation. At 42 days of gestation, 50 multiparous sows were randomly allocated to one of two treatments, namely a diet containing pork lard (n = 25) and a diet containing soybean oil (n = 25). The fats were provided at 3.6% during gestation and at 4% during lactation. The experimental diets were offered through the weaning of the piglets. The fatty acid profile of the milk was determined fourteen days after parturition. At weaning (21 days postpartum) and seven days later, one of the piglets (n = 64) from 16 sows allocated to each treatment was selected at random to determine fatty acid profiles in brain, skin and muscle. Saturated and monounsaturated fatty acids were higher in the diet with pork lard than in that with soybean oil, in which the polyunsaturated fat content was higher. A higher saturation of fatty acids was found in milk from the sows that consumed pork lard, which contained more saturated fatty acids than the milk from sows that consumed soybean oil. The fatty acid profiles in muscle and skin of the piglets were affected by the diet of the sows. However, the fatty acid profile of the piglets’ brains was not affected by the diet of their mothers. Keywords: fat saturation, lard, piglet survival, sow feeding, soybean oil


2010 ◽  
Vol 55 (No. 11) ◽  
pp. 479-487 ◽  
Author(s):  
L. Bartoň ◽  
D. Bureš ◽  
V. Kudrna

The effects of breed and diet containing different types of silages on meat quality parameters and fatty acid profile of m. longissimus lumborum (MLL) were evaluated in a total of 30 Czech Fleckvieh (CF), Charolais (CH) and Charolais &times; Czech Fleckvieh (CH &times; CF) bulls. The animals were fed two mixed diets: MS (based on maize silage) and LCS (based on legume-cereal mixture silage and lucerne silages) with different concentrations of dietary energy and fatty acids. The MLL from CH bulls had the lowest content of dry matter (P &lt; 0.01), less protein (P &lt; 0.01) and lighter meat (P &lt; 0.01) compared to the CF. The extensive LCS diet reduced dry matter (P &lt; 0.01) and intramuscular fat (P &lt; 0.01) and increased the content of hydroxyproline (P &lt; 0.05). The CH bulls exhibited higher PUFA n-3 (P &lt; 0.05) and lower MUFA (P &lt; 0.05) compared to the CF, with the CH &times; CF being intermediate. The LCS diet enhanced the proportions of PUFA (P &lt; 0.05) and PUFA n-3 (P &lt; 0.001) and reduced MUFA (P &lt; 0.001). In conclusion, both breed and diet affected the meat quality and fatty acid profile of the intramuscular fat of the bulls. The replacement of maize silage with the legume-cereal mixture and lucerne silages in the diet reduced the concentration of intramuscular fat and improved its fatty acid profile from the human nutrition perspective.


2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 254-254
Author(s):  
Linnea Rimmer ◽  
Cassandra K Jones

Abstract Due to the rising goat population in the United States, there is growing economic incentive to feed dried distillers grains with solubles (DDGS) over soybean meal (SBM). However, there is limited knowledge of how this may affect carcass composition. Therefore, the objective of this experiment was to evaluate the impact of titrating levels of DDGS as a replacement of SBM on Boer goat fatty acid profiles. Thirty-two Boer goat kids were fed isocaloric and isonitrogenous diets consisting of 0%, 33%, 66%, and 100% DDGS replacing SBM. There were no detected differences (P &gt; 0.05) in final body weight after a 47-d growth experiment. Goats were slaughtered in a commercial, USDA-inspected facility, backfat samples collected between the 12th and 13th rib, and samples analyzed for fatty acid profile via AOAC Official Method 996.06. Data were analyzed with goat as the experimental unit in a completely randomized design. Dietary treatment did not impact (P &gt; 0.05) the overall fatty acid profile or iodine value (50 to 52 points) of fat samples. This may be because fatty acids go through biohydrogenation by rumen bacteria, limiting the impact of diet on fatty acid profile. That said, increasing levels of DDGS impacted (P = 0.03) both C16:1 and C18:3 in a quadratic manner. Specifically, goats fed 0% and 100% DDGS replacing SBM had higher C16:1 than those fed intermediate levels of DDGS, while the opposite was true for C18:3. No other fatty acid was affected (P &gt; 0.05), nor were there shifts in the ratio of saturated vs. unsaturated, or the total sum of n-3 fatty acids. In summary, fatty acid profiles are not significantly impacted by increasing levels of DDGS in place of SBM, and therefore DDGS can be used in Boer goat diets without impacting fat quality.


HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 487b-487
Author(s):  
William R. Nail ◽  
J. Benton Storey ◽  
L.J. Grauke

Pollen of pecan [Carya illinoinensis (Wangenh.) K. Koch] from five protandrous and five protogynous genotypes was analyzed for germinability and fatty acid profile. Fatty acid profiles as a function of genotype were distinctive in all selections, but only three of the genotypes had fatty acid profiles that were easily distinguishable from others by canonical discriminant analysis. Specific fatty acid percentages influenced germination in some genotypes. Total saturated and unsaturated fatty acids influenced pollen germination to a greater degree than individual fatty acids. The effects of fatty acids on pollen germination of protandrous genotypes were opposite those on `Stuart', the only protogynous genotype to be influenced by fatty acid percentages.


2014 ◽  
Vol 83 (4) ◽  
pp. 371-378 ◽  
Author(s):  
Stanisław Milewski ◽  
Cezary Purwin ◽  
Barbara Pysera ◽  
Krzysztof Lipiński ◽  
Zofia Antoszkiewicz ◽  
...  

Recently, the quality of lamb meat has been regarded with attention to the content of intramuscular fat, and its fatty acid profile. These indicators are determined by the feed administered to the animals. Apparently, the type of silage used in animal nutrition may be important. The objective of this study was to determine the effect of feeding lambs with silages produced from different plant species on selected meat quality traits. The experiment was conducted on 24 ram lambs, divided into 3 equal groups fed with red clover silage, alfalfa silage, and grass silage, respectively. After 60 days of fattening, the lambs were slaughtered. Samples collected from musculus longissimus lumborum were assayed for: contents of intramuscular fat, cholesterol, the vitamins retinol and α-tocopherol, and the fatty acid profile of intramuscular fat. The meat of lambs fed red clover silage was characterized by a higher content of fat compared to lambs receiving grass silage (P ≤ 0.05). The concentration of vitamin E in the group fed grass silage was the highest and differed significantly (P ≤ 0.01) compared to the group fed alfalfa silage. Intramuscular fat of lambs fed red clover silage contained more polyunsaturated fatty acids n-3 and was characterized by a lower ratio of polyunsaturated fatty acids n-6:n-3 compared to the lambs fed grass silage. The concentration of conjugated linoleic acid was higher in lambs fed grass silage compared to fat of lambs from the other groups (P ≤ 0.05). Feeding lambs with silages produced from various plant species had a positive effect on traits characterizing the health quality of meat. Until now, only a few studies have been conducted in this species; this is the first such comprehensive study in sheep.


2019 ◽  
Vol 40 (6Supl3) ◽  
pp. 3629
Author(s):  
Regis Luis Missio ◽  
Luis Fernando Glasenapp de Menezes ◽  
Ivan Luiz Brondani ◽  
José Laerte Nörnberg ◽  
Paulo Santana Pacheco ◽  
...  

The objective of this study was to evaluate the fatty acid profile of duodenal digesta (experiment I) and of meat of beef cattle (experiment II) fed diets containing different levels of concentrate (220, 400, 590 and 790 g of concentrate/kg of dry matter of the diets). The experiment I was conducted with four Charolais-Nellore steers (460 ± 18.2 kg of BW), with a T-shaped duodenal cannula, using a double 4 × 4 Latin square as an experimental design. In experiment II, 16 crossbred Charolais-Nellore young bulls (192.44 ± 18.2 kg of BW) were randomly distributed in the experimental treatments (220, 400, 590 and 790 g of concentrate/kg of dry matter of the diets). The diets were isonitrogenous (120 g of crude protein/kg of dry matter). The intramuscular fat content was used as a covariant for the statistical analysis of the meat fatty acid profile. The duodenal content of fatty acid C17:0 decreased with increase of concentrate levels, while its content in the meat presented a quadratic variation with the increase of the concentrate levels of the diets, being the lowest values observed for the diet with 400 g of concentrate. The duodenal content of fatty acid C18:1 trans-11 decreased, whereas the content of this fatty acid in the meat increased with the increase of the dietary concentrate levels. The increase in the level of concentrate reduced the content of polyunsaturated fatty acids C18:3 n-3, C20:3 n-6, C20:4 n-6, and C20:5 n-3 EPA in both the duodenal digesta and meat. No difference was observed in the n-6/n-3 fatty acids ratio (mean of 13.96) of the meat between diets. The elevation of the level of concentrate in confinement diets reduces the nutraceutical quality of the meat of Charolais-Nellore young bulls slaughtered at 14-16 months of age due to the reduction of the polyunsaturated fatty acids content important for human health.


2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Zenon Nogalski ◽  
Martyna Momot ◽  
Paulina Pogorzelska-Przybyłek ◽  
Monika Sobczuk-Szul ◽  
Monika Modzelewska-Kapituła

Abstract The aim of this study was to determine the effect of Virginia fanpetals (Sida hermaphrodita) silage on the fatty acid profile and the content of selected nutrients and vitamins in the Longissimus lumborum (LL) and Semimembranosus (SM) muscles of young bulls. Forty Polish Holstein-Friesian bulls aged 16 months were assigned to four dietary treatments (n=10) and were fed different types of silage during a 7-month fattening period. The proportion (g/kg dry matter) of silage in the diets was as follows: (1) grass silage (GS) (600); (2) Virginia fanpetals silage (VFS) (600); (3) VFS (300) and GS (300); and (4) VFS (300) and maize silage (MS) (300). Silage was supplemented with concentrate at 400 g/kg DM in each diet. The animals were slaughtered at the end of the fattening period. The intramuscular fat (IMF) of bulls fed GS had the highest (P<0.05) concentrations of n-3 polyunsaturated fatty acids (PUFAs) and linolenic acid (LNA), whereas the IMF of bulls receiving GS and VFS was characterized by the highest proportion of MUFAs, mostly oleic acid (C18:1 cis 9). In comparison with the LL muscle, the SM muscle contained less IMF (by 40%) with a more nutritionally desirable profile. The SM muscle was characterized by a more desirable mineral composition and a higher concentration of α-tocopherol.


2021 ◽  
Vol 8 ◽  
Author(s):  
Manuel Oliva-Cruz ◽  
Pati Llanina Mori-Culqui ◽  
Aline C. Caetano ◽  
Malluri Goñas ◽  
Nuri C. Vilca-Valqui ◽  
...  

Cocoa beans are the raw material for the chocolate industry. In this study, the total fat contents and fatty acid profiles of fine-aroma cocoa beans of 30 cocoa ecotypes from northeastern Peru were evaluated. Results showed that SJJ-1 and ACJ-11 ecotypes from San Martin and Amazonas regions, respectively, presented highest percentages of total fat with an average of 30.49%. With respect to fatty acid profiles, it was found that cocoa ecotypes are composed of 10 fatty acids (C14:0, C16:0, C16:1, C18:0, C17:0, C18:1, C18:2, C18:3, C20:0, and C22:0); based on this profile, 5 clusters were determined. Cluster 5 had the highest content of C17:0 fatty acid (0.47%); however, the clusters 1, 2, 3, and 4 had the lowest content of this fatty acid (0.37%, 0.32%, 0.32%, respectively). The clusters 3 and 4 showed the highest content of C16:0 fatty acid (31.13% y 28.97%, respectively). The clusters 3 and 5 contained the highest content of the acid C18:1 (27.08% y 26.82%, respectively). The PCA found that C18:0 and C20:0 fatty acids are correlated, and are fundamentally opposite to C18:1, C16:0, and C18:3 acids. These results may be useful in identifying raw material for the development of specialty chocolates with better nutritional value than traditional cocoa.


2016 ◽  
Vol 16 (3) ◽  
pp. 909-929 ◽  
Author(s):  
Daniel Franco ◽  
Mirian Pateiro ◽  
Diego Rois ◽  
Jose Antonio Vázquez ◽  
José Manuel Lorenzo

AbstractThe effects of caponization on growth performance, carcass characteristics, meat quality and fatty acid profile of breast and drumstick of Mos and Sasso genotypes, reared in free-range production system were examined. A total of 47 birds of Mos breed (19 Castrated and 25 Entire) and 37 of Sasso X-44 (18 Castrated and 19 Entire) slaughtered at 32 weeks were used in this trial. The growth of broilers and the differences between genotypes and caponization effects were modelled by Weibull-growth equation. Capon’s growth was higher than that obtained by roosters and Sasso weight was greater than Mos results (P<0.05). For both genotypes the chemical composition of breast and drumstick cuts showed significantly higher values of intramuscular fat (P<0.0001) and lesser values of moisture (P<0.0001) in capons in comparison with intact ones. In Mos breed, capons exhibited significantly (P<0.0001) higher values of breast and drumstick luminosity and yellowness, as well as lower values of redness. Regarding Warner-Braztler test (WB), there were no significant differences (P>0.05) by caponization effect, but hardness measured using textural profile analysis was lesser in meat from capons. Finally castration of animals led to important modifications in the intramuscular fat because meat from capons showed significantly lower values for total saturated fatty acids (SFA) and higher polyunsaturated fatty acids (PUFA ). Nutritional indices were also more favorable in capon’s meat, so overall fatty acid profile of capons was desirable from the viewpoint of human nutrition.


2020 ◽  
Author(s):  
Vivian Vezzoni Almeida ◽  
Julia Pereira Martins da Silva ◽  
Ariana Nascimento Meira ◽  
Gabriel Costa Monteiro Moreira ◽  
Julia Dezen Gomes ◽  
...  

Abstract Background There has been an increased interest in nutritional strategies to manipulate the fatty acid profile of pigs. Dietary regimens involving the use of oils that are high in monosaturated fatty acid (MUFA), primarily oleic acid (OA), such as canola oil (CO), as well as in omega (n)-3 polyunsaturated fatty acid (PUFA), which are found in fish oil (FO), have been investigated aiming healthier fatty acid profile cuts, with a higher ratio of n-3 to n-6 fatty acids. Therefore, the effects of including 3% soybean oil (SO), CO, or FO in growing-finishing pig diets vs. feeding a standard commercial diet with 1.5% SO (control) on growth performance, carcass traits, meat quality, consumer acceptability, and intramuscular fatty acid composition of the longissimus lumborum (LL) muscle were evaluated. Results Dietary treatments had no effect on overall growth performance and pig carcasses. Although loins from pigs fed diets containing either 3% SO or CO showed a reduction (P = 0.05) in Warner-Bratzler shear force, only the addition of 3% SO to pig diets resulted in loin chops that were rated higher (P < 0.001) for consumer overall liking. Adding either 3% SO or CO increased (P < 0.01) the percentages of OA and total MUFA in the LL intramuscular fat compared to control- or FO-fed pigs. However, intramuscular fat from 3% SO- or CO-fed pigs had the lowest (P < 0.01) proportion of total n-3 PUFA than control- or FO-fed pigs. Including 3% fat, regardless of source, reduced (P < 0.01) total PUFA, total n-6 PUFA, and PUFA:saturated fatty acid (SFA) ratio than control-fed pigs. Dietary FO inclusion decreased (P < 0.01) n-6:n-3 PUFA ratio, but also increased total SFA (P < 0.01) and atherogenic index (P = 0.02) in the LL intramuscular fat. Conclusions Although adding 3% CO or FO to pig diets provided slight nutritional benefits to consumers in terms of MUFA and long chain n-3 PUFA contents, respectively, formulating growing-finishing diets with 1.5% SO was adequate enough in terms of LL intramuscular fatty acids composition for high quality meat destined to human consumption.


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