Diet and Physical Activity as Determinants of Continuously Measured Glucose Levels in Persons at High Risk of Type 2 Diabetes

We examined how dietary and physical activity behaviors influence fluctuations in blood glucose levels over a seven-day period in people at high risk for diabetes. Twenty-eight participants underwent a mixed meal tolerance test to assess glucose homeostasis at baseline. Subsequently, they wore an accelerometer to assess movement behaviors, recorded their dietary intakes through a mobile phone application, and wore a flash glucose monitoring device that measured glucose levels every 15 min for seven days. Generalized estimating equation models were used to assess the associations of metabolic and lifestyle risk factors with glycemic variability. Higher BMI, amount of body fat, and selected markers of hyperglycemia and insulin resistance from the meal tolerance test were associated with higher mean glucose levels during the seven days. Moderate- to vigorous-intensity physical activity and polyunsaturated fat intake were independently associated with less variation in glucose levels (CV%). Higher protein and polyunsaturated fatty acid intakes were associated with more time-in-range. In contrast, higher carbohydrate intake was associated with less time-in-range. Our findings suggest that dietary composition (a higher intake of polyunsaturated fat and protein and lower intake of carbohydrates) and moderate-to-vigorous physical activity may reduce fluctuations in glucose levels in persons at high risk of diabetes.

Reproducibility and validity ELSA-Brasil Food Frequency Questionnaire

Background: To investigate the association between diet and health effects in population surveys, it is necessary to have precise tools that allow estimating the habitual consumption of the population. Introduction: To investigate the association between diet and health effects in population surveys, it is necessary to have precise tools that allow estimating the habitual consumption of the population. Aim: To evaluate the reproducibility and validity of the ELSA-Brasil food frequency questionnaire (FFQ) to assess macro and micronutrients intake. Material and methods: We collected dietary information of 281 participants which completed two ELSA-Brasil food frequency questionnaires (FFQ) over a year period and three records. To assess the reproducibility of the FFQ, we compared the macro and micronutrients intake from the two FFQ while to assess the validity, we compared the intakes of FFQ with the mean of three records. The intraclass correlation test (ICC) and agreement percentages of nutrient intake were calculated after categorization by tertiles. Results: ICC coefficients for reproducibility ranged from 0.51 (polyunsaturated fat) to 0.70 (magnesium) while the ICC coefficients for validity ranged from 0.14 to 0.61 for omega 3 and magnesium, respectively. The exact concordances between methods ranged from 37% for omega 3 to 50.2% for magnesium (mean = 44.6%). An average disagreement of 13.4% was found. Conclusions: This study suggests that the ELSA-Brasil FFQ is suitable tool to assess dietary intake with a satisfactory reproducibility and relative validity.

Dietary Fat and Protein Intakes in Relation to Plasma Sphingolipids As Determined by a Large-Scale Lipidomic Analysis

Objectives Sphingolipid concentrations have been associated with risk of type 2 diabetes and cardiovascular diseases. Because sphingolipids can be synthesized de novo from saturated fatty acids (SFA), dietary fatty acids may affect plasma sphingolipid concentrations. We aimed to evaluate dietary fat and protein intakes in relation to circulating sphingolipid levels. Methods We used cross-sectional data from 2860 ethnic Chinese Singaporeans collected from 2004–2007. Nutrient intakes were estimated on the basis of a validated 159-item food frequency questionnaire. We quantified 79 molecularly distinct sphingolipids in a large-scale lipidomic evaluation from plasma samples. Results Higher saturated fat intake was associated with higher concentrations of 16:1; O2 sphingolipids including ceramides, monohexosylcermides, dihexosylceramides, sphingomyelins, and sphingosine 1-phosphates. Higher polyunsaturated fat intake was associated with lower plasma long-chain ceramides and long-chain monohexosylcermide concentrations. Protein intake was inversely associated with concentrations of most subclasses of sphingolipids, with the exception of sphingolipids containing a 16:1; O2 sphingoid base. Lower intake of saturated fat and higher intake of polyunsaturated fat and protein may decrease plasma concentrations of several sphingolipid classes. Conclusions These findings may represent a novel biological mechanism for the impact of nutrient intakes on cardio-metabolic health. Funding Sources This work was supported by the National Research Foundation Investigatorship grant (NRF-NRFI2015–05, to MRW), A*STAR (I1901E0040), and the National University Health System (NUHSRO/2014/085/AF-Partner/01, DRH). FT was supported by the NRF and A*STAR IAF-ICP I1901E0040.

Adiponectin Gene Variant rs3774261, Effects on Lipid Profile and Adiponectin Levels after a High Polyunsaturated Fat Hypocaloric Diet with Mediterranean Pattern

The role of ADIPOQ gene variants on metabolic improvements after weight change secondary to different hypocaloric diets remained unclear. We evaluate the effect of rs3774261 of ADIPOQ gene polymorphism on biochemical improvements and weight change after high polyunsaturated fat hypocaloric diet with a Mediterranean dietary pattern for 12 weeks. A population of 361 obese subjects was enrolled in an intervention trial with a calorie restriction of 500 calories over the usual intake and 45.7% of carbohydrates, 34.4% of fats, and 19.9% of proteins. The percentages of different fats was; 21.8% of monounsaturated fats, 55.5% of saturated fats, and 22.7% of polyunsaturated fats. Before and after intervention, an anthropometric study, an evaluation of nutritional intake and a biochemical evaluation were realized. All patients lost weight regardless of genotype and diet used. After 12 weeks with a similar improvement in weight loss (AA vs. AG vs. GG); total cholesterol (delta: −28.1 ± 2.1 mg/dL vs. −14.2 ± 4.1 mg/dL vs. −11.0 ± 3.9 mg/dL; p = 0.02), LDL cholesterol (delta: −17.1 ± 2.1 mg/dL vs. −6.1 ± 1.9 mg/dL vs. −6.0 ± 2.3 mg/dL; p = 0.01), triglyceride levels (delta: −35.0 ± 3.6 mg/dL vs. 10.1 ± 3.2 mg/dL vs. −9.7 ± 3.1 mg/dL; p = 0.02), C reactive protein (CRP) (delta: −2.3 ± 0.1 mg/dL vs. −0.2 ± 0.1 mg/dL vs. −0.2 ± 0.1 mg/dL; p = 0.02), serum adiponectin (delta: 11.6 ± 2.9 ng/dL vs. 2.1 ± 1.3 ng/dL vs. 3.3 ± 1.1 ng/dL; p = 0.02) and adiponectin/leptin ratio (delta: 1.5 ± 0.1 ng/dL vs. 0.3 ± 0.2 ng/dL vs. 0.4 ± 0.3 ng/dL; p = 0.03), improved only in AA group. AA genotype of ADIPOQ variant (rs3774261) is related with a significant increase in serum levels of adiponectin and ratio adiponectin/leptin and decrease on lipid profile and C-reactive protein (CRP).

Comparison of nutritional status between lacto-ovo vegetarian and non-vegetarian Jordanian adults

Purpose Vegetarian diets exclude meat, fish and poultry and/or egg and dairy products, these diets are based on grains, fruits, vegetables, legumes and seeds. The purpose of this study is to compare the nutritional status between lacto-ovo vegetarian and non-vegetarian Jordanian adults. Design/methodology/approach A case-control study was conducted during the period between (April–November 2019). In total, 200 Jordanians in early adulthood aged between 18 and 35 years participated in the present study; 100 subjects were non-vegetarians and 100 subjects were lacto-ovo vegetarians. The ratio was (1:1). Matching between the two groups was done in terms of age, sex and body mass index. A package that consisted of three structured questionnaires: Personal Information Sheet, Food Frequency Questionnaire and Seven-Day Physical Activity Recall were administered to all participants in this study. Findings The concentration of serum vitamin B12 was significantly higher (P = 0.011) in non-vegetarians than lacto-ovo vegetarians. The means of intake of calories (P = 0.003), calories from fat (P = 0.001), calories from saturated fat (P = 0.001), protein (P = 0.001), fat (P = 0.001), saturated fat (P = 0.001), monounsaturated fat (P = 0.022), polyunsaturated fat (P = 0.001), cholesterol (P = 0.001) and omega-6 (P = 0.039) were significantly higher in non-vegetarians. The intakes Mean of carbohydrates (P = 0.001), fiber (P = 0.001) and soluble fiber (P = 0.001) were significantly higher in lacto-ovo vegetarians compared to non-vegetarians. The mean of beta-carotene intake was significantly higher (P = 0.001) in lacto-ovo vegetarians compared to non-vegetarians, although the intakes of vitamin A(RAE) and retinol were significantly higher (P = 0.029, P = 0.001, respectively) in non-vegetarians as compared to lacto-ovo vegetarians. The means of vitamins B2 (P = 0.018), B3 (P = 0.001), B3NE (P = 0.001), B6 (mg) (P = 0.001), B12 (P = 0.001), E-a-Tocopherol (P = 0.001) and D (P = 0.001) intake were significantly higher in non-vegetarians compared to lacto-ovo vegetarians. The mean intakes of vitamins C (P = 0.033), folate (P = 0.005) and K (P = 0.002) were significantly in lacto-ovo vegetarians compared to non-vegetarians. Means intake of some minerals was significantly higher in non-vegetarians than lacto-ovo vegetarians. Originality/value The current study showed that lacto-ovo vegetarians had lower serum vitamin B12 levels. The consumption of fruits, vegetables and legumes was higher in lacto-ovo vegetarians than non-vegetarians. While lacto-ovo vegetarian diet provided less fat, saturated fat, polyunsaturated fat, monounsaturated fat and cholesterol than non-vegetarians, it could be considered a rich source for fiber, folate, beta-carotene, vitamin C and vitamin K.

Dietary Fat and Protein Intake in Relation to Plasma Sphingolipids as Determined by a Large-Scale Lipidomic Analysis

Sphingolipid concentrations have been associated with risk of type 2 diabetes and cardiovascular diseases. Because sphingolipids can be synthesized de novo from saturated fatty acids (SFA), dietary fatty acids may affect plasma sphingolipid concentrations. We aimed to evaluate dietary fat and protein intakes in relation to circulating sphingolipid levels. We used cross-sectional data from 2860 ethnic Chinese Singaporeans collected from 2004–2007. Nutrient intakes were estimated on the basis of a validated 159-item food frequency questionnaire. We quantified 79 molecularly distinct sphingolipids in a large-scale lipidomic evaluation from plasma samples. Higher saturated fat intake was associated with higher concentrations of 16:1;O2 sphingolipids including ceramides, monohexosylcermides, dihexosylceramides, sphingomyelins, and sphingosine 1-phosphates. Higher polyunsaturated fat intake was associated with lower plasma long-chain ceramides and long-chain monohexosylcermide concentrations. Protein intake was inversely associated with concentrations of most subclasses of sphingolipids, with the exception of sphingolipids containing a 16:1;O2 sphingoid base. Lower intake of saturated fat and higher intake of polyunsaturated fat and protein may decrease plasma concentrations of several sphingolipid classes. These findings may represent a novel biological mechanism for the impact of nutrient intakes on cardio-metabolic health.

Dietary Intake and Gastrointestinal Integrity in Runners Undertaking High-Intensity Exercise in the Heat

Gastrointestinal disturbances are one of the most common issues for endurance athletes during training and competition in the heat. The relationship between typical dietary intake or nutritional interventions and perturbations in or maintenance of gut integrity is unclear. Twelve well-trained male endurance athletes (peak oxygen consumption = 61.4 ± 7.0 ml·kg−1·min−1) completed two trials in a randomized order in 35 °C (heat) and 21 °C (thermoneutral) conditions and kept a detailed nutritional diary for eight consecutive days between the two trials. The treadmill running trials consisted of 15 min at 60% peak oxygen consumption, 15 min at 75% peak oxygen consumption, followed by 8 × 1-min high-intensity efforts. Venous blood samples were taken at the baseline, at the end of each of the three exercise stages, and 1 hr postexercise to measure gut integrity and the permeability biomarker concentration for intestinal fatty-acid-binding protein, lipopolysaccharide, and lipopolysaccharide-binding protein. The runners self-reported gut symptoms 1 hr postexercise and 3 days postexercise. The heat condition induced large (45–370%) increases in intestinal fatty-acid-binding protein, lipopolysaccharide-binding protein, and lipopolysaccharide concentrations compared with the baseline, but induced mild gastrointestinal symptoms. Carbohydrate and polyunsaturated fat intake 24 hr preexercise were associated with less lipopolysaccharide translocation. Protein, carbohydrate, total fat, and polyunsaturated fat intake (8 days) were positively associated with the percentage increase of intestinal fatty-acid-binding protein in both conditions (range of correlations, 95% confidence interval = .62–.93 [.02, .98]). Typical nutrition intake partly explained increases in biomarkers and the attenuation of symptoms induced by moderate- and high-intensity exercise under both heat and thermoneutral conditions.

Nutrient Intake and Dietary Quality Among Children and Adolescents by Fast Food Consumption Status: What We Eat in America, NHANES 2013–2016

Objectives To categorize children and adolescents by frequency of fast food (FF) consumption and compare intakes of energy, nutrients, and dietary quality among those with and without intake of fast food. Methods One day of dietary intake data from What We Eat in America, NHANES 2013–2016 were analyzed. The sample included children and adolescents age 2–5 y (N = 1152), 6–11 y (N = 1804), and 12–19 y (N = 2072). FF consumption on intake day included at least one reported food or beverage with the source indicated as “restaurant fast food/pizza”. Based on self-reported frequency of FF consumption in the previous seven days, participants were categorized as: Infrequent (0 times) (IFF), frequent (1 + times) without report of FF source (FF-NC) or frequent (1 + times) with report of FF source (FF-C) on the intake day. Differences between groups in energy, nutrient intake, and diet quality using the Healthy Eating Index (HEI) 2015, were assessed using T-tests in a multiple regression adjusting for confounders. Differences were considered significant at P < 0.001. Results There were no significant differences in energy or nutrient intakes by FF status among children 2–5 y. However, their total HEI scores (of possible 100) were higher in IFF (57) and FF-NC (54) vs FF-C (50); subcomponent scores of IFF were higher than FF-C and FF-NC for Refined Grains, and higher for Whole Fruit and Total Protein Foods than FF-C. Among children 6–11 y, IFF had lower intake of polyunsaturated fat and higher intake of vitamin D than FF-C, but no differences in HEI scores. Among adolescents, IFF and FF-NC had lower intakes of energy, carbohydrate, total fat, polyunsaturated fat, monounsaturated fat, and sodium than FF-C and total HEI scores were higher in IFF (51) and FF-NC (49) vs FF-C (45). Subcomponent scores of IFF were higher for Total and Whole Fruit, Whole Grains, and Added Sugars than FF-C and higher for Total Fruit in FF-NC vs FF-C. There were no differences in nutrient intake or diet quality between IFF and FF-NC in any age group. Conclusions Differences in nutrient intakes and diet quality by FF consumption status varied among children and adolescents. These differences highlight the need to tailor nutrition education and messaging by age. Funding Sources U.S. Department of Agriculture.