scholarly journals Co-inoculation of Bradyrhizobium japonicum and Azospirillum brasilense on the physiological quality of soybean seeds

2020 ◽  
Vol 41 (6supl2) ◽  
pp. 2937-2950
Author(s):  
Paloma Helena da Silva Libório ◽  
◽  
Ivana Marino Bárbaro-Torneli ◽  
Marco Antonio Nogueira ◽  
Sandra Helena Unêda-Trevisoli ◽  
...  

The success of the soybean crop depends on the physiological quality of seeds, which can be favored by beneficial microorganisms, however, it may be impaired by unfavorable environmental conditions. The aim of this study was to evaluate the effect of co-inoculation with Bradyrhizobium japonicum and Azospirillum brasilense on the physiological quality of soybean seeds obtained in the 2017/2018 crop season, from a field trial involving 23 cultivars submitted to co-inoculation (in-furrow) or without coinoculation. Plants were assessed for nodulation at R1 and, after harvest at R8, seeds were assessed for concentration of proteins, mass of thousand seeds, and seed physiological quality [(Germination, emergence of seedlings in sand, and Emergence Speed Index (ESI)]. In the average of cultivars, the number of nodules per plant increased from 36.0 in the control to 44.4 nodules with co-inoculation. Increases in the concentration of proteins and in the mass of thousand seeds due to co-inoculation were 5.6% and 34.7%, respectively. Seeds originated from co-inoculated plants had higher germination rate at the first (50% vs. 45.3%) and at the final (87% vs. 79.8%) countings, in addition to higher rate of seedlings emergence in sand box (83.3% vs. 80%), and higher ESI (18.5 vs. 17.4). The benefits of coinoculation were observed in 17 of 23 cultivars (74%) for at least two of the seven assessed variables. Considering only the minimal germination of 80%, the seeds originated from 10 non-inoculated cultivars could not be used, whereas for the co-inoculated plants this number fell to four.

2021 ◽  
Vol 24 (5-esp.) ◽  
pp. 576-583
Author(s):  
Jorge González Aguilera ◽  
Alan Mario Zuffo ◽  
Rafael Felippe Ratke ◽  
Renato Jaqueto Goes ◽  
Natielly Pereira Da Silva ◽  
...  

O emprego de produtos biológicos no sistema de plantio direto constitui-se uma prática sustentável e com menor potencial poluente que o uso de produtos químicos. Nesta pesquisa objetivou-se avaliar o desempenho agronômico de três cultivares de soja após a inoculação pós emergente de biológicos no solo. O delineamento experimental utilizado foi o de blocos casualizados em esquema em parcelas subdivididas 3×5, com três repetições. Os tratamentos foram a combinação de três cultivares (BRASMAX-FOCO-IPRO, BRASMAX-DESAFIO-IPRO e BRASMAX-BÔNUS-IPRO) e quatro biológicos [Azospirillum brasilense + Bradyrhizobium japonicum (1), BioAx (2), Trichoderma asperrellum (3), 1+2+3 (4)], mais um Controle (ausência de biológicos) (5). Foi avaliado a altura das plantas (AP), inserção do primeiro legume (IPL), número de legume por planta (PNL), número de grãos por legumes (NGL), massa de mil grãos (MMG) e produtividade (PROD). Os grãos colhidos, foram classificados por tamanho em peneiras de diâmetros de 7.5, 7.0, 6.5, 6.0, 5.5, 5.0 e 4.5 mm. A porcentagem de grãos retidos (RP) e a produtividade relativa de grãos retidos nas peneiras (PRGRP) foi calculada. Os resultados apontaram interações significativas (p<0,01) para as variáveis NGL, NGP, MMG, Ø6.5, Ø6.0, Ø5.5, Ø5.0, RP e PRGRP. O emprego de biológicos aplicados de modo post-emergente promove uma melhor qualidade fisiológica das sementes de soja se comparadas ao controle, ao considerar os diâmetros de Ø6.0 e Ø6.5.   Palavras-chave: Glycine max. Qualidade das Sementes. Azospirillum brasilense; Bradyrhizobium japonicum. BioAx; Trichoderma asperrellum.   Abstract The use of biological products in the no-tillage system is a sustainable practice with less polluting potential than the use of chemicals. This research aimed to evaluate the agronomic performance of three soybean cultivars after the post-emergence inoculation of biologicals in the soil. The experimental design used was randomized blocks in a  3 × 5 subdivided plots scheme, with three replications. The treatments were the combination of three cultivars (BRASMAX-FOCO-IPRO, BRASMAX-DESAFIO-IPRO and BRASMAX-BÔNUS-IPRO) and four biological [Azospirillum brasilense + Bradyrhizobium japonicum (1), BioAx (2), Trichoderma asperrellum (3) , 1 + 2 + 3 (4)], plus a Control (absence of biological products) (5). Plant height (PH), insertion of the first pods (IFP), number of pods per plant (NPP), number of grains per pods (NGP), mass of one thousand grains (MTG) and productivity (PROD) were evaluated. The harvested grains were classified by size in sieves with diameters of 7.5, 7.0, 6.5, 6.0, 5.5, 5.0 and 4.5 mm. The percentage of grains retained (GR) and the relative productivity of grains retained in the sieves (RPGRS) was calculated. The results showed significant interactions (p<0.01) for the variables NGP, NPP, MTG, Ø6.5, Ø6.0, Ø5.5, Ø5.0, GR and RPGRS. The use of biologically applied post-emergence promotes a better physiological quality of soybean seeds compared to the control, when considering the diameters of Ø6.0 and Ø6.5.   Keywords: Glycine max. quality of seeds. Azospirillum brasilense. Bradyrhizobium japonicum. BioAx; Trichoderma asperrellum.  


2018 ◽  
Vol 48 (3) ◽  
pp. 261-270 ◽  
Author(s):  
Alan Mario Zuffo ◽  
Fábio Steiner ◽  
Aécio Busch ◽  
Joacir Mario Zuffo Júnior ◽  
Alan Eduardo Seglin Mendes ◽  
...  

ABSTRACT Nitrogen fertilization may interfere in the quality of soybean seeds. This study aimed to determine the physiological and sanitary quality of soybean seeds, in response to levels and times of nitrogen (N) application associated with Bradyrhizobium japonicum inoculation. Treatments were arranged in a randomized block design, in a 2 × 3 × 4 factorial arrangement [two soybean cultivars (BRS 1074 IPRO and ST 797 IPRO), three application times (sowing, 30 and 50 days after emergence) and four N levels (0 kg ha-1, 20 kg ha-1, 40 kg ha-1 and 60 kg ha-1), with four replicates. The 1,000-seed mass, moisture content, germination rate, seedling height, shoot length, primary root length, total seedling dry matter, electrical conductivity, mechanical damage, vigor and viability (tetrazolium test) and sanitary quality of the seeds were measured. The use of mineral N, associated with the inoculation of B. japonicum bacteria, does not prove to be consistent, in terms of its effects on the physiological quality of soybean seeds, although the incidence of pathogens may be reduced.


2017 ◽  
Vol 39 (2) ◽  
pp. 174-181 ◽  
Author(s):  
Cristian Rafael Brzezinski ◽  
Julia Abati ◽  
Fernando Augusto Henning ◽  
Ademir Assis Henning ◽  
José de Barros França Neto ◽  
...  

Abstract: The aim of this work was to evaluate the effect of different spray volumes, via industrial treatment, on the physiological quality of soybean seeds with different levels of vigor. The experimental design was completely randomized in a 2x5 factor scheme, with four replications. Factors consisted in two levels of seed vigor (high and low) and five spray volumes (0, 600, 1200, 1800 and 2400 mL. 100 kg-1). Products used to obtain the volumes were: fungicide (carbendazin + thiram); insecticide (imidacloprid + thiodicarb); nematicide (abamectin); micronutrients (cobalt, molybdenum and zinc); polymer (peridiam); biostimulants (kinetin + gibberellic acid) and inoculum (Bradyrhizobium japonicum). The used cultivars were BRS 360 RR and BRS 1010 IPRO. The physiological seed quality was determined by the following evaluations: germination, first count of the germination test, seedling emergence in sand, emergency speed index, total length of shoot and root of the seedlings. In the different spray volumes, high vigor soybean seeds have higher physiological quality than low vigor seeds. The increased spray volumes in seed treatments reduce the physiological quality of low vigor soybean seeds.


Author(s):  
André F. Capilheira ◽  
Jerffeson A. Cavalcante ◽  
Gizele I. Gadotti ◽  
Bruna R. Bezerra ◽  
Nander F. Hornke ◽  
...  

ABSTRACT The characteristics of materials used in packages for seed storage may have a negative influence in the deterioration process, causing future problems during the period of storage and in emergence in the field. In this study, the objective was to evaluate the physiological quality of soybean seeds, stored in different packages with and without the addition of CO2 (modified atmosphere), for different periods of storage and in uncontrolled environmental conditions. In order to carry out the experiment, a completely randomized design was used in a split-plot scheme, with three types of packages (permeable, hermetic package inside the permeable package and hermetic package inside the permeable package with CO2 injection) in the main plot and six storage periods (zero, 45, 90, 135, 180 and 225 days) in the subplot, with four repetitions, totaling 72 experimental units. In hermetic packages multi-layer paper was used with or without CO2 injection, stored them for 225 days and performed an assessment at regular intervals of 45 days. At each period, the seeds were submitted to tests for moisture content, germination, seedling emergence, accelerated aging and electrical conductivity. The hermetic package, with and without CO2 injection, decreases the speed of deterioration of soybean seeds. The hermetic package allows a higher physiological quality of soybeans compared to the permeable package, with a storage period of up to 180 days under uncontrolled environmental conditions. The addition of CO2 inside the hermetic packages favors the maintenance of the physiological quality of soybean seeds in storage.


Author(s):  
Julia Abati ◽  
Cristian Rafael Brzezinski ◽  
Claudemir Zucareli ◽  
Flávia Werner ◽  
Ademir Assis Henning ◽  
...  

The industrial treatment of seeds has favored the development of new technologies such as the use of dry powder. It has made application of spray volumes higher than those used in the conventional treatment. Therefore, this study aimed to assess the effect of industrial treatments at different spray volumes with and without application of dry powder on physiological quality of soybean seeds. A completely randomized experimental design was performed with four replications in a 5 x 2 factorial arrangement for cultivars BRS 359 RR and BRS 284. The factors were five spray volumes (0, 600, 1200, 1800 and 2400 mL. 100 kg-1) with and without the application of dry powder. The products used to obtain the volumes were fungicides (carbendazim + thiram); insecticides (imidacloprid + thiodicarb); nematicide (abamectin); micronutrients (cobalt, molybdenum and zinc); polymer (peridiam); biostimulant (kinetin + gibberellic acid + 4-indol-3-ylbutyric acid) and inoculant (Bradyrhizobium japonicum). The physiological quality of the seeds was determined by the following assessments: germination, first count of germination test, seedling emergence in sand, emergence speed index, and total length of the seedling shoot and root. The results showed that increased spray volume associated with the application of dry powder in seed treatment reduced the physiological quality of soybean seeds, and spray volumes up to 1200 mL 100 kg-1 in seed treatment without the application of dry powder do not impact the emergence and establishment of soybean seedlings.


2015 ◽  
Vol 28 (4) ◽  
pp. 190-201 ◽  
Author(s):  
FÁBIO PALCZEWSKI PACHECO ◽  
LÚCIA HELENA PEREIRA NÓBREGA ◽  
GISLAINE PICOLLO DE LIMA ◽  
MÁRCIA SANTORUM ◽  
WALTER BOLLER ◽  
...  

ABSTRACT: The mechanical harvesting causes injuries on seeds and may affect their quality. Different threshing mechanisms and their adjustments may also affect the intensity of impacts that machines cause on seeds. So, this study aimed at diagnosing and evaluating the effect of two combines: the first one with a threshing system of axial flow and the other one with a threshing system of tangential flow, under adjustments of concave opening (10 mm, 30 mm and 10 mm for a combine with axial flow and 3.0 mm, 15 mm and 3.0 mm for a combine with tangential flow) and three cylinder rotations on the quality of soybean seeds harvested at two moisture contents. Soybean seeds of cultivar 'ND 4910' were harvested at 16.6% moisture (mid-morning) and 13.7% moisture in the afternoon. The seeds quality was evaluated by germination tests, germination speed index (GSI), germination rate, moisture content, percentage of purity and vigor by tetrazolium test. Despite the combine, the results showed that the mechanical injury has most reduced seeds quality, at 16.6% moisture content, concave opening of 30 mm (axial) and 10 mm (tangential) and cylinder rotation of 1100 rpm (axial) and 1000 (tangential), both with the highest rotations used. The combine with tangential flow had the highest degree of seeds purity. When seeds moisture content at harvest was close to 13.7%, there was the highest seed injury, while, at 16.6%, there was the highest number of crushed soybeans, regardless the combine adjustment.


2017 ◽  
Vol 11 (02) ◽  
pp. 162-168
Author(s):  
Amanda Ribeiro Peres ◽  
◽  
Claudinei Kappes ◽  
José Roberto Portugal ◽  
Marco Eustáquio de Sá ◽  
...  

Author(s):  
Camila Andressa Bissaro ◽  
Rafael Oliveira Defendi ◽  
Lucas Caiubi Pereira ◽  
Alessandro Lucca Braccini ◽  
Diogo Francisco Rossoni ◽  
...  

2018 ◽  
Vol 10 (5) ◽  
pp. 312
Author(s):  
Geliandro Anhaia Rigo ◽  
Luis Osmar Braga Schuch ◽  
Willian Silva Barros ◽  
Rodrigo Lamaison de Vargas ◽  
Vinícius Jardel Szareski ◽  
...  

The aim of this work was to correlate macronutrient content of soybean seeds to physiological quality of different cultivars. The work was developed in the Federal University of Pelotas, in the facilities of the Seed Science and Technology Graduate Program. The experimental design was randomized blocks in arranged in four replicates. The following soybean cultivars were used: BMX Apolo RR (12 lots), BMX Ativa RR (13 lots), BMX Energia RR (26 lots), BMX Força RR (24 lots), BMX Impacto RR (35 lots), BMX Magna RR (16 lots), BMX Turbo RR (44 lots), BMX Potência RR (82 lots) and NA 5909 RR (28 lots), with seeds produced in northwest Rio Grande do Sul. The determination of nutritional contents found in plant tissues of soybean seeds, were measured: Nitrogen (N), Phosphorus (P), Potassium (K), Magnesium (Mg), Calcium (Ca), Sulfur (S). The macronutrient contents of the seeds vary according to genetic characteristics of the cultivars, with higher oscillations of nitrogen, phosphorus, magnesium and sulfur contents present in soybean seeds. Potassium and calcium are defined as the most stable nutrients for the cultivars and seed lots analyzed. High concentrations of nitrogen, phosphorus and calcium are determinants for the physiological quality of soybean seeds.


2018 ◽  
Vol 39 (2) ◽  
pp. 855 ◽  
Author(s):  
Janete Denardi Munareto ◽  
Thomas Newton Martin ◽  
Tania Maria Müller ◽  
Ubirajara Russi Nunes ◽  
Guilherme Bergeijer da Rosa ◽  
...  

Seed treatment is a practice that helps the initial establishment of the crop without the effects caused by pests and diseases. The association of diazotrophic bacteria with grasses has been used in the supply of nitrogen to plants; however, these microorganisms produce growth-promoting substances, which promote benefits in the growth and development of the crops. Thus, the objective of this study was to evaluate the compatibility of Azospirillum brasilense associated with the fungicide difenoconazole and the insecticide thiamethoxam by observing the effects on the quality of seed emergence of three wheat cultivars (Triticum aestivum L.). Three wheat cultivars, arranged in a 4 x 2 factorial system with four replicates, were tested. The treatments were the control; difenoconazole (Spectro® at a dose of 150 mL per 100 kg of seed); thiamethoxam (Cruiser® FS 350 at a dose of 200 mL per 100 kg seed) and difenoconazole + thiamethoxam, applied on wheat cultivars TBIO Mestre, TBIO Itaipu and TBIO Sinuelo. Bacteria from the Azospirillum genus were used in the inoculation. The wheat seed retains its quality when it checks the germination, vigor and independent accelerated aging, whether or not fungicide, insecticide and A. brasilense were used. The insecticide thiamethoxam increased the length of shoots and roots and provided compatibility with A. brasilense, and fungicide inhibited the length of shoots and roots and was antagonistic to the bacterium A. brasilense.


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