Co-inoculation and inoculation methods of plant growth-promoting bacteria in wheat yield performance

Several studies have reported the beneficial effects of inoculation of Azospirillum brasilense in wheat, but only a few of them have related the co-inoculation of A. brasilense and Rhizobium sp. and the evaluation of different inoculation methods. This study aimed i) to verify the efficiency of plant growth-promoting bacteria (PGPB) in subtropical environments, ii) to verify the efficiency of co-inoculation of A. brasilense and Rhizobium sp., and iii) to verify the efficiency of the management of different inoculation methods in the wheat crop. The experiments were carried out in Londrina and Apucarana, State of Paraná, Brazil, under a complete randomized block design, with four replications and nine treatments: T1) absence of nitrogen (N) topdressing, T2) 30 kg ha−1 of N topdressing, T3) 60 kg ha−1 of N topdressing, T4) A. brasilense Ab-V5 in the seeds, T5) A. brasilense Ab-V5 in post-emergence, T6) A. brasilense Ab-V5 + Rhizobium sp. 53GRM1 in the seeds, T7) A. brasilense Ab-V5 + Rhizobium sp. 53GRM1 in post-emergence, T8) commercial inoculant (A. brasilense Ab-V5 + Ab-V6) in the seeds, and T9) commercial inoculant (A. brasilense Ab-V5 + Ab-V6) in post-emergence. The number of ears per linear meter, number of spikelets, number of grains per spikelet, number of grains per ear, thousand-grain weight, number of spikelets to ears ratio, leaf nitrogen content, and grain yield were evaluated. Leaf N content and yield components showed no alterations due to the inoculation and co-inoculation performed both in the seed and in the post-emergence of seedlings. Treatments T3, T7, and T9 showed the highest means of grain yield (2077.50, 1743.12, and 1660.62 kg ha−1, respectively), demonstrating that co-inoculation with A. brasilense Ab-V5 + Rhizobium sp. 53GRM1 and inoculation with A. brasilense Ab-V5 + Ab-V6, both in post-emergence of seedlings, have the potential to replace the topdressing nitrogen fertilization in wheat.

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A Multifactorial Approach to Untangle Graphene Oxide (GO) Nanosheets Effects on Plants: Plant Growth-Promoting Bacteria Inoculation, Bacterial Survival, and Drought

Nanomaterials ◽

10.3390/nano11030771 ◽

2021 ◽

Vol 11 (3) ◽

pp. 771

Author(s):

Tiago Lopes ◽

Catarina Cruz ◽

Paulo Cardoso ◽

Ricardo Pinto ◽

Paula A. A. P. Marques ◽

...

Keyword(s):

Graphene Oxide ◽

Plant Growth ◽

Food Production ◽

Plant Growth Promoting Bacteria ◽

Bacterial Survival ◽

Maize Seedlings ◽

Combined Application ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Rhizobium Sp

Drought is a limiting factor for agricultural productivity. Climate change threatens to expand the areas of the globe subjected to drought, as well as to increase the severity and duration of water shortage. Plant growth-promoting bacteria (PGPB) are widely studied and applied as biostimulants to increase plant production and to enhance tolerance to abiotic and biotic constraints. Besides PGPB, studies on the potential of nanoparticles to be used as biostimulants are also thriving. However, many studies report toxicity of tested nanoparticles in bacteria and plants in laboratory conditions, but few studies have reported effects of nanoparticles towards bacterial cells and communities in the soil. The combined application of nanoparticles and PGPB as biostimulant formulations are poorly explored and it is important to unravel the potentialities of their combined application as a way to potentiate food production. In this study, Rhizobium sp. E20-8 and graphene oxide (GO) nanosheets were applied on container-grown maize seedlings in watered and drought conditions. Bacterial survival, seedling growth (dry weight), and biochemical endpoints (photosynthetic pigments, soluble and insoluble carbohydrates, proline, lipid peroxidation, protein, electron transport system, and superoxide dismutase) were evaluated. Results showed that the simultaneous exposure to GO and Rhizobium sp. E20-8 was able to alleviate the stress induced by drought on maize seedlings through osmotic and antioxidant protection by GO and mitigation of GO effects on the plant’s biochemistry by Rhizobium sp. E20-8. These results constitute a new lead on the development of biostimulant formulations to improve plant performance and increase food production in water-limited conditions.

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Rhizobacteria Isolated from Saline Soil Induce Systemic Tolerance in Wheat (Triticum aestivum L.) against Salinity Stress

Agronomy ◽

10.3390/agronomy10070989 ◽

2020 ◽

Vol 10 (7) ◽

pp. 989 ◽

Cited By ~ 2

Author(s):

Noshin Ilyas ◽

Roomina Mazhar ◽

Humaira Yasmin ◽

Wajiha Khan ◽

Sumera Iqbal ◽

...

Keyword(s):

Salt Stress ◽

Plant Growth ◽

Salinity Stress ◽

Plant Biomass ◽

Growth Promotion ◽

Soluble Sugar ◽

Wheat Crop ◽

Plant Growth Promoting Bacteria ◽

Plant Growth Promoting ◽

Growth Promoting

Halo-tolerant plant growth-promoting rhizobacteria (PGPR) have the inherent potential to cope up with salinity. Thus, they can be used as an effective strategy in enhancing the productivity of saline agro-systems. In this study, a total of 50 isolates were screened from the rhizospheric soil of plants growing in the salt range of Pakistan. Out of these, four isolates were selected based on their salinity tolerance and plant growth promotion characters. These isolates (SR1. SR2, SR3, and SR4) were identified as Bacillus sp. (KF719179), Azospirillum brasilense (KJ194586), Azospirillum lipoferum (KJ434039), and Pseudomonas stutzeri (KJ685889) by 16S rDNA gene sequence analysis. In vitro, these strains, in alone and in a consortium, showed better production of compatible solute and phytohormones, including indole acetic acid (IAA), gibberellic acid (GA), cytokinin (CK), and abscisic acid (ABA), in culture conditions under salt stress. When tested for inoculation, the consortium of all four strains showed the best results in terms of improved plant biomass and relative water content. Consortium-inoculated wheat plants showed tolerance by reduced electrolyte leakage and increased production of chlorophyll a, b, and total chlorophyll, and osmolytes, including soluble sugar, proline, amino acids, and antioxidant enzymes (superoxide dismutase, catalase, peroxidase), upon exposure to salinity stress (150 mM NaCl). In conclusion, plant growth-promoting bacteria, isolated from salt-affected regions, have strong potential to mitigate the deleterious effects of salt stress in wheat crop, when inoculated. Therefore, this consortium can be used as potent inoculants for wheat crop under prevailing stress conditions.

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Maize response to inoculation with strains of plant growth-promoting bactéria

Revista Brasileira de Engenharia Agrícola e Ambiental ◽

10.1590/1807-1929/agriambi.v20n7p606-611 ◽

2016 ◽

Vol 20 (7) ◽

pp. 606-611 ◽

Cited By ~ 4

Author(s):

Janaína Dartora ◽

Vandeir F. Guimarães ◽

Cid R. J. Menezes ◽

Mariângela B. Freiberger ◽

Gustavo Castoldi ◽

...

Keyword(s):

Plant Growth ◽

Block Design ◽

N Fertilization ◽

Plant Growth Promoting Bacteria ◽

Maize Seeds ◽

Randomized Block Design ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Maize Response ◽

Rhizobium Sp

ABSTRACT The aim of this study was to evaluate the response of maize to inoculation with strains of plant growth-promoting bacteria (PGPB) in two cultivation years. The experiment was set in a randomized block design with four replicates in two cultivation years (2012/13 and 2013/14). The treatments consisted of PGPB inoculation: control (without N and without inoculation); 30 kg of N ha-1 at sowing (N1); 160 kg of N ha-1 (N1 + 130 kg of N ha-1 as top-dressing); N1 + A. brasilense, Ab-V5; N1 + A. brasilense, HM053; N1 + Azospirillum sp. L26; N1 + Azospirillum sp. L27; N1 + Enhydrobacter sp. 4331; N1 + Rhizobium sp. 8121. Basal stem diameter, plant height, leaf area, shoot dry matter and yield were evaluated. The strain of Rhizobium sp. 8121and the isolate Azospirillum sp. L26 associated with 30 kg of N ha-1 at sowing promoted yields equivalent to that of the N fertilization of 160 kg ha-1, demonstrating the potential to be used in the inoculation of maize seeds.

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Plant growth-promoting bacteria associated with nitrogen fertilization at topdressing in popcorn agronomic performance

Bragantia ◽

10.1590/1678-4499.330 ◽

2016 ◽

Vol 75 (1) ◽

pp. 33-40 ◽

Cited By ~ 8

Author(s):

Leandro Teodoski Spolaor ◽

Leandro Simões Azeredo Gonçalves ◽

Odair José Andrade Pais dos Santos ◽

André Luiz Martinez de Oliveira ◽

Carlos Alberto Scapim ◽

...

Keyword(s):

Plant Growth ◽

Grain Yield ◽

Nitrogen Fertilization ◽

Block Design ◽

N Fertilization ◽

Plant Growth Promoting Bacteria ◽

Promising Alternative ◽

Plant Growth Promoting ◽

Growth Promoting ◽

N Rates

ABSTRACT The use of plant growth-promoting bacteria is a promising alternative with low environmental impact to increase the efficiency of use of chemical fertilizers, ensuring high yield with better cost-effective ratio. In maize crops, several studies have demonstrated an increased yield when Azospirillum-based inoculants are used. In the case of popcorn, there are no available studies related to use of inoculation and its response on yield parameters. Thus, the aim of this study was to evaluate the field performance of popcorn when inoculated with the commercial product Masterfix L (A. brasilense Ab-V5 and A. brasilense Ab-V6) and the non-commercial inoculant UEL (A. brasilense Ab-V5 + Rhizobium sp. 53GRM1) associated with nitrogen fertilization. The trials were conducted in Londrina and Maringá, Paraná State, Brazil, in a randomized block design with four replications, in a split plot design with the inoculation treatments located in the plots (uninoculated, Masterfix L, and UEL) and the different N rates located in the subplots where ammonium sulphate was applied in the topdressing at the V6 stage (0, 50, 100, and 150 kg∙ha–1). The variance analysis showed significant effects (p < 0.05) of inoculation (Londrina environment) and N rates (both environments) only for grain yield. There was no inoculation effect in the grain yield when inoculants were applied together with N-fertilization at topdressing. In the absence of N-fertilization at topdressing, the inoculants Masterfix L. and UEL promoted higher grain yield as compared to the uninoculated plants, with resulting increases of 13.21 and 26.61% in yield, respectively.

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Impact of plant growth-promoting bacteria on grain yield, protein content, and urea-15 N recovery by maize in a Cerrado Oxisol

Plant and Soil ◽

10.1007/s11104-017-3193-1 ◽

2017 ◽

Vol 422 (1-2) ◽

pp. 239-250 ◽

Cited By ~ 23

Author(s):

Marcio Reis Martins ◽

Claudia Pozzi Jantalia ◽

Verônica Massena Reis ◽

Ingbert Döwich ◽

José Carlos Polidoro ◽

...

Keyword(s):

Plant Growth ◽

Grain Yield ◽

Protein Content ◽

N Recovery ◽

Plant Growth Promoting Bacteria ◽

Plant Growth Promoting ◽

Growth Promoting

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Isolation and identification of plant growth-promoting bacteria associated with tall fescue

Proceedings of the New Zealand Grassland Association ◽

10.33584/jnzg.2009.71.2751 ◽

2009 ◽

pp. 211-216 ◽

Cited By ~ 5

Author(s):

J. Monk ◽

E. Gerard ◽

S. Young ◽

K. Widdup ◽

M. O'Callaghan

Keyword(s):

New Zealand ◽

Plant Growth ◽

Tall Fescue ◽

Festuca Arundinacea ◽

Plant Growth Promoting Bacteria ◽

Beneficial Bacteria ◽

Isolation And Identification ◽

Naturally Occurring ◽

Plant Growth Promoting ◽

Growth Promoting

Tall fescue (Festuca arundinacea) is a useful alternative to ryegrass in New Zealand pasture but it is slow to establish. Naturally occurring beneficial bacteria in the rhizosphere can improve plant growth and health through a variety of direct and indirect mechanisms. Keywords: rhizosphere, endorhiza, auxin, siderophore, P-solubilisation

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