scholarly journals Chorioallantoic Membrane (CAM) Assay of Different Extracts of Rhizome and Inflorescence of Heliconia rostrata

2018 ◽  
Vol 52 (4s) ◽  
pp. S246-S251
Author(s):  
Moonmun Dhara ◽  
Lopamudra Adhikari ◽  
Raja Majumder
Keyword(s):  
Chorioallantoic Membrane ◽  
Cam Assay

scholarly journals Pro-angiogenic and osteogenic composite scaffolds of fibrin, alginate and calcium phosphate for bone tissue engineering

2021 ◽  
Vol 12 ◽  
pp. 204173142110056
Author(s):  
Nupur Kohli ◽  
Vaibhav Sharma ◽  
Alodia Orera ◽  
Prasad Sawadkar ◽  
Nazanin Owji ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Chorioallantoic Membrane ◽  
Cam Assay ◽  
Clinical Model ◽  
Chick Chorioallantoic Membrane ◽  
Biomimetic Method ◽  
Alginate Scaffold

Due to the limitations of bone autografts, we aimed to develop new composite biomaterials with pro-angiogenic and osteogenic properties to be used as scaffolds in bone tissue engineering applications. We used a porous, cross-linked and slowly biodegradable fibrin/alginate scaffold originally developed in our laboratory for wound healing, throughout which deposits of calcium phosphate (CaP) were evenly incorporated using an established biomimetic method. Material characterisation revealed the porous nature and confirmed the deposition of CaP precursor phases throughout the scaffolds. MC3T3-E1 cells adhered to the scaffolds, proliferated, migrated and differentiated down the osteogenic pathway during the culture period. Chick chorioallantoic membrane (CAM) assay results showed that the scaffolds were pro-angiogenic and biocompatible. The work presented here gave useful insights into the potential of these pro-angiogenic and osteogenic scaffolds for bone tissue engineering and merits further research in a pre-clinical model prior to its clinical translation.

scholarly journals O-033. A modified chorioallantoic membrane (CAM) assay for testing anti-angiogenic strategies in a model of experimentally induced endometriosis

1999 ◽  
Vol 14 (Suppl_3) ◽  
pp. 18-19
Author(s):  
Ph. Kressin ◽  
E. Wolber ◽  
A. Meyhöfer-Malik ◽  
K. Diedrich ◽  
E. Malik
Keyword(s):  
Chorioallantoic Membrane ◽  
Cam Assay ◽  
Experimentally Induced

scholarly journals Patient Derived Chicken Egg Tumor Model (PDcE Model): Current Status and Critical Issues

Cells ◽  
2019 ◽  
Vol 8 (5) ◽  
pp. 440 ◽  
Author(s):  
Aoi Komatsu ◽  
Kotaro Matsumoto ◽  
Tomoki Saito ◽  
Manabu Muto ◽  
Fuyuhiko Tamanoi
Keyword(s):  
Cancer Patients ◽  
Chorioallantoic Membrane ◽  
Tumor Model ◽  
Tumor Formation ◽  
Current Status ◽  
Cam Assay ◽  
Chicken Egg ◽  
Critical Issues ◽  
Chicken Eggs ◽  
Chorioallantoic Membrane Assay

Chorioallantoic membrane assay (CAM assay) using fertilized chicken eggs has been used for the study of tumor formation, angiogenesis and metastasis. Recently, there is growing realization that this system provides a valuable assay for a patient-derived tumor model. Several reports establish that tumor samples from cancer patients can be used to reproduce tumor in the chicken egg. High transplantation efficiency has been achieved. In this review, we discuss examples of transplanting patient tumors. We then discuss critical issues that need to be addressed to pursue this line of experiments. The patient-derived chicken egg model (PDcE model) has an advantage over other models in its rapid tumor formation. This raises the possibility that the PDcE model is valuable for identifying optimum drug for each individual patient.

scholarly journals In Vivo Modulation of Angiogenesis and Immune Response on a Collagen Matrix via Extracorporeal Shockwaves

2020 ◽  
Vol 21 (20) ◽  
pp. 7574 ◽  
Author(s):  
Diana Heimes ◽  
Nadine Wiesmann ◽  
Jonas Eckrich ◽  
Juergen Brieger ◽  
Stefan Mattyasovszky ◽  
...  
Keyword(s):  
Gene Expression ◽  
Immune Response ◽  
Chorioallantoic Membrane ◽  
Extracorporeal Shock Wave Therapy ◽  
Collagen Matrix ◽  
Cam Assay ◽  
Immunological Responses ◽  
Tissue Integration ◽  
Extracorporeal Shock Wave

The effective management of tissue integration and immunological responses to transplants decisively co-determines the success of soft and hard tissue reconstruction. The aim of this in vivo study was to evaluate the eligibility of extracorporeal shock wave therapy (ESWT) with respect to its ability to modulate angiogenesis and immune response to a collagen matrix (CM) for tissue engineering in the chorioallantoic membrane (CAM) assay, which is performed with fertilized chicken eggs. CM were placed on the CAM on embryonic development day (EDD) 7; at EDD-10, ESWT was conducted at 0.12 mJ/mm2 with 500 impulses each. One and four days later, angiogenesis represented by vascularized area, vessel density, and vessel junctions as well as HIF-1α and VEGF gene expression were evaluated. Furthermore, immune response (iNOS2, MMP-9, and MMP-13 via qPCR) was assessed and compared between ESWT- and non-ESWT-groups. At EDD-14, the vascularized area (+115% vs. +26%) and the increase in vessel junctions (+751% vs. +363%) were significantly higher in the ESWT-group. ESWT significantly increased MMP-9 gene expression at EDD-11 and significantly decreased MMP-13 gene expression at EDD-14 as compared to the controls. Using the CAM assay, an enhanced angiogenesis and neovascularization in CM after ESWT were observed. Furthermore, ESWT could reduce the inflammatory activity after a latency of four days.

scholarly journals Evaluation of the Anti-angiogenic Activity of Saponins from Maesa lanceolata by Different Assays

2012 ◽  
Vol 7 (9) ◽  
pp. 1934578X1200700
Author(s):  
Kenn Foubert ◽  
Annelies Breynaert ◽  
Mart Theunis ◽  
Rita Van Den Bossche ◽  
Guido R.Y. De Meyer ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Chorioallantoic Membrane ◽  
Rat Aorta ◽  
Cam Assay ◽  
Plant Metabolites ◽  
Angiogenic Activity ◽  
Test Systems ◽  
Aorta Ring

Angiogenesis, in which a vascular network is established from pre-existing vessels, is a complex multistep process. Mechanisms underlying angiogenesis can be investigated using a variety of in vitro, ex vivo and in vivo approaches. Evaluation of several promising plants and plant metabolites, including terpenoids, revealed promising anti-angiogenic activity. Since the maesasaponins displayed anti-angiogenic activity in the chick chorioallantoic membrane (CAM) assay, their activity was further investigated in several test systems. The rat aorta ring assay was compared with the placental vein assay and then selected for the ex vivo investigation of the saponins. Besides their effect on the viability of HUVEC, the anti-angiogenic capacity of the compounds was also investigated in an in vivo zebrafish assay. The activity of the saponins in the viability assay was more pronounced than in the rat aorta ring assay and similar to the effect observed in the CAM assay. The use of different test systems, however, implies different results in the case of saponins.

scholarly journals Nymphaeol-A Isolated from Okinawan Propolis Suppresses Angiogenesis and Induces Caspase-Dependent Apoptosis via Inactivation of Survival Signals

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Ikumi Tsuchiya ◽  
Takahiro Hosoya ◽  
Motoko Ushida ◽  
Kazuhiro Kunimasa ◽  
Toshiro Ohta ◽  
...  
Keyword(s):  
Screening Program ◽  
Biological Activities ◽  
Umbilical Vein ◽  
Chorioallantoic Membrane ◽  
Tube Formation ◽  
Mitogen Activated Protein Kinase ◽  
Cam Assay ◽  
Prenylated Flavonoids

Propolis, a resinous substance that honeybees collect to protect their beehive from enemies, is reported to have various biological activities. In our screening program to search for antiangiogenic compounds from propolis, the ethanol extracts of Okinawan propolis (EEOP) showed significant antiangiogenic activities in a tube formation assay with human umbilical vein endothelial cells (HUVECs)in vitroat 3.13 μg/mL and chorioallantoic membrane (CAM) assayin vivoat 25 μg/egg. To elucidate the active compounds of EEOP and their mode of action, we isolated some prenylated flavonoids from EEOP and found that nymphaeol-A had the strongest antiangiogenic activity among them. Nymphaeol-A significantly reducedin vivoneovessel formation in the CAM assay at 25 μg/egg. At the molecular level, nymphaeol-A markedly inactivated mitogen-activated protein kinase/ERK kinase 1/2 (MEK1/2) and extracellular signal-regulated kinase 1/2 (ERK1/2), whose molecular activations signal new vessel formation in HUVECs. In addition, nymphaeol-A dose- and time-dependently induced caspase-dependent apoptosis in tube-forming HUVECs. Taken together, nymphaeol-A was shown to inhibit angiogenesis at least in part via inactivation of MEK1/2–ERK1/2 signaling and induction of caspase-dependent apoptosis. Okinawan propolis and its major component, nymphaeol-A, may be useful agents for preventing tumor-induced angiogenesis.

scholarly journals Evaluation of pharmacological effect of Teucrium stocksianum extract on angiogenesis using chorioallantoic membrane assay

2016 ◽  
Vol 11 (3) ◽  
pp. 621
Author(s):  
Nazia Tabassum ◽  
Alamgeer . ◽  
Abdul Aziz ◽  
Bashir Ahmad
Keyword(s):  
Blood Vessels ◽  
Plant Extract ◽  
Chorioallantoic Membrane ◽  
Pharmacological Effect ◽  
Cam Assay ◽  
Antiangiogenic Effect ◽  
Teucrium Stocksianum ◽  
Chorioallantoic Membrane Assay

<p class="Abstract">The present study was aimed to evaluate the effect of <em>Teucrium stocksianum</em> on angiogenesis by using chorioallantoic membrane (CAM) assay. Fertilized eggs were incubated on the 5<sup>th</sup> day and dose of different dilutions 0.03%, 0.05%, 0.1%, and 0.5% of the plant extract was applied on 6<sup>th</sup> day. Evaluation of primary, secondary and tertiary blood vessels diameter and CAM area on 7<sup>th</sup> day by SPIP software. <em>T. stocksianum</em> showed antiangiogenic effect by reducing the diameter of CAM of blood vessels by applying the dilutions while significant results were obtained at dilution of 0.5%.</p><p> </p><p><strong> </strong></p>

scholarly journals Extending the Applicability of In Ovo and Ex Ovo Chicken Chorioallantoic Membrane Assays to Study Cytostatic Activity in Neuroblastoma Cells

2021 ◽  
Vol 11 ◽  
Author(s):  
Miguel Angel Merlos Rodrigo ◽  
Berta Casar ◽  
Hana Michalkova ◽  
Ana Maria Jimenez Jimenez ◽  
Zbynek Heger ◽  
...  
Keyword(s):  
High Throughput Screening ◽  
Neuroblastoma Cells ◽  
Chorioallantoic Membrane ◽  
Stage Iv ◽  
Metastatic Potential ◽  
Significant Inhibition ◽  
Cam Assay ◽  
Anticancer Activities ◽  
In Ovo

PurposeThe chick chorioallantoic membrane (CAM) assay can provide an alternative versatile, cost-effective, and ethically less controversial in vivo model for reliable screening of drugs. In the presented work, we demonstrate that CAM assay (in ovo and ex ovo) can be simply employed to delineate the effects of cisplatin (CDDP) and ellipticine (Elli) on neuroblastoma (Nbl) cells in terms of their growth and metastatic potential.MethodsThe Nbl UKF-NB-4 cell line was established from recurrent bone marrow metastases of high-risk Nbl (stage IV, MYCN amplification, 7q21 gain). Ex ovo and in ovo CAM assays were optimized to evaluate the antimetastatic activity of CDDP and Elli. Immunohistochemistry, qRT-PCR, and DNA isolation were performed.ResultsEx ovo CAM assay was employed to study whether CDDP and Elli exhibit any inhibitory effects on growth of Nbl xenograft in ex ovo CAM assay. Under the optimal conditions, Elli and CDDP exhibited significant inhibition of the size of the primary tumor. To study the efficiency of CDDP and Elli to inhibit primary Nbl tumor growth, intravasation, and extravasation in the organs, we adapted the in ovo CAM assay protocol. In in ovo CAM assay, both studied compounds (CDDP and Elli) exhibited significant (p &lt; 0.001) inhibitory activity against extravasation to all investigated organs including distal CAM.ConclusionsTaken together, CAM assay could be a helpful and highly efficient in vivo approach for high-throughput screening of libraries of compounds with expected anticancer activities.

Sign in / Sign up

Export Citation Format

Share Document