Formulation of Traditional Mask Powder Containing the Mixture of Coffea robusta, Angelica keiskei and Oryzae sativa, and its Activity as Tyrosinase Enzyme Inhibitor

Objective: The aim of this study was to formulate and evaluate the efficacy of Angelica keiskei (AK) leaf extract in serum gel as an antioxidant and tyrosinase enzyme inhibitor. Methods: The leaves of AK were extracted using the maceration method, while the antioxidant and tyrosinase enzyme inhibitory activities were determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and L-Tyrosine as assay and substrate, respectively. Subsequently, the extract was formulated into serum gel and evaluated for physical stability, antioxidant and inhibitory activity to tyrosinase enzyme. Results: The results showed that AK leaf extract has antioxidant activity, with an IC50 value = 7.73 mg/ml, while the tyrosinase enzyme inhibitory power had an IC50 value = 500 mg/ml. Based on physical stability, the serum gel was stable after 28 d of storage. The serum gel of AK leaf extract also showed antioxidant activity and tyrosinase enzyme inhibition with IC50 value of 16.68 mg/ml and 741.62 mg/ml, respectively. Conclusion: This study demonstrated that the serum gel of AK leaf extract has potential as a skin lightening agent and is safe for use as a topical preparation.

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FORMULATION OF THE SERUM GEL CONTAINING GREEN COFFEE BEAN (Coffea robusta L) EXTRACT AS AN ANTIOXIDANT AND TYROSINASE ENZYME INHIBITOR

RASAYAN Journal of Chemistry ◽

10.31788/rjc.2020.1345866 ◽

2020 ◽

Vol 13 (04) ◽

pp. 2346-2351

Author(s):

D. L. Aulifa ◽

M. Caroline ◽

D. Tristiyanti ◽

A. Budiman

Keyword(s):

Enzyme Inhibitor ◽

Coffee Bean ◽

Green Coffee ◽

Tyrosinase Enzyme ◽

Coffea Robusta ◽

Green Coffee Bean

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Ashitaba (Angelica keiskei) leaves extract cream 8% inhibited the increase of melanin amount as effective as hydroquinone cream 4% and inhibited the increase of tyrosinase enzymes not as effective as hydroquinone cream 4% in the ultraviolet B-exposed Guin

Neurologico Spinale Medico Chirurgico ◽

10.36444/nsmc.v4i1.147 ◽

2021 ◽

Vol 4 (1) ◽

pp. 22-27

Author(s):

Chynthia Friama ◽

Anak Agung Gde Putra Wiraguna ◽

Wimpie Pangkahila

Keyword(s):

Guinea Pig ◽

Sun Exposure ◽

Ultraviolet B ◽

Premature Aging ◽

Control Group ◽

Cavia Porcellus ◽

Control Group Design ◽

Angelica Keiskei ◽

The Mean ◽

Tyrosinase Enzyme

Background: Excessive sun exposure results in increased activity of the tyrosinase enzyme and the amount of melanin in the skin which causes hyperpigmentation, a sign of premature aging. The purpose of this study was to prove that Ashitaba (Angelica keiskei) leaves extract cream 8% inhibited the increase of melanin and tyrosinase enzymes as effective as hydroquinone cream 4% in the ultraviolet B-exposed guinea pig (Cavia porcellus) skin. Methods: This study used a randomized post-test only control group design. The subjects were 36 male guinea pigs (Cavia porcellus). Subjects were divided into two groups, the hydroquinone cream 4% group and the Ashitaba leaves extract cream 8% group exposed to UVB light. Skin samples were collected 48 hours after the last exposure to measure the tyrosinase enzyme levels using the ELISA method and the amount of melanin was examined by Masson-Fontana staining. Results: The mean levels of tyrosinase in the hydroquinone cream 4% group was 19.51 ± 5.16 ng/L and the Ashitaba leaves extract cream 8% group was 23.76 ± 3.09 ng/L (p = 0.005). The mean amount of melanin in the hydroquinone cream 4% group was 2.98 ± 2.27% and the Ashitaba leaves extract cream 8% group was 4.77 ± 3.33% (p = 0.069). Conclusion: The administration of Ashitaba (Angelica keiskei) leaves extract cream 8% inhibited the increase of melanin amount as effective as hydroquinone cream 4% and inhibited the increase of tyrosinase enzymes not as effective as hydroquinone cream 4% in the ultraviolet B-exposed guinea pig (Cavia porcellus) skin.

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FORMULATION, STABILTY TEST AND ENZYME ACTIVITY TEST TYROSINASE OF CREAM PEPARATIONS FROM HERENDONG FRUIT EXTRACT(Melastoma affine D. Don)

INDONESIA NATURAL RESEARCH PHARMACEUTICAL JOURNAL ◽

10.52447/inspj.v5i2.4451 ◽

2020 ◽

Vol 5 (2) ◽

pp. 149-173

Author(s):

Zuraida Sagala ◽

Kurnia Telaumbanua

Keyword(s):

Enzyme Inhibitors ◽

Enzyme Inhibitor ◽

Mechanical Test ◽

Ethanol Extract ◽

Activity Test ◽

Irritation Test ◽

Ic50 Values ◽

Tyrosinase Enzyme ◽

Ethanol Extracts ◽

The Stability

One way to prevent or inhibit melanin formation is by inhibiting tyrosinase activity (Lloyd,2011). Tyrosinase enzyme is an enzyme that plays a role in the formation of skin pigments or known as melanogenesis. In the process of melanogenesis, the enzyme tyrosinase acts as a catalyst in two different reactions, namely the hydroxylation process of tyrosine to dihydroxy-phenylalanine (L-DOPA) and oxidation of L-DOPA to quinone DOPA. Tyrosinase in skin tissue is activated by solar UV radiation so that it accelerates the process of melanin production (Fais et al. 2009). This study was conducted to determine the stability, effectiveness of cream preparations from ethanol extracts of Harendong fruit (Melastoma affine D. Don) as tyrosinase enzyme inhibitors so it can be used as a cosmetic ingredient for whitening or skin lightening. Results Cream with variants of Harendong (Melastoma affine D. Don) ethanol extract was physically stable and formulated with organoleptic test, homogeneity, viscosity, mechanical test, pH, cycling test, and irritation test. Type M / A cream preparations from ethanol extract of Harendong fruit (Melastoma affine D. Don) have a strong / moderate tyrosinase enzyme inhibitor activity with IC50 values of F1 (1%) of 526,192 ppm, F2 (1.5%) ) amounted to 317,534 ppm and F3 (2%) amounted to 128,523 ppm. Keywords: Foemulation, stability test, activity test, tyrosinase enzyme, herenong fruit (Melastoma affine G.Don)

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THE RECENT UPDATE OF DEOXYARBUTIN: A SKIN DEPIGMENTATION AGENT WITH TYROSINASE INHIBITION TARGETING

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2020v12i3.36957 ◽

2020 ◽

pp. 1-7

Author(s):

MUCHTARIDI MUCHTARIDI ◽

MENTARI LUTHFIKA DEWI

Keyword(s):

Kojic Acid ◽

Enzyme Inhibitor ◽

Clinical Manifestations ◽

Tyrosinase Inhibitor ◽

Tyrosinase Inhibition ◽

Considerable Potential ◽

Abnormal Accumulation ◽

Tyrosinase Enzyme ◽

The Stability ◽

Further Development

Melanin is produced through the process of melanogenesis, which serves to protect the skin from the damaging effects of UV radiation. Abnormal accumulation of melanin will aesthetically disturb even interfere with health. One of the clinical manifestations of abnormal accumulation of melanin is the incidence of melasma. Some of the tyrosinase enzyme inhibitor agents most widely used as Hydroquinone, Kojic acid and Arbutin do not give satisfactory results and cause serious side effects. Hydroquinone is known to cause ochronosis exogenous and cytotoxic. Kojic acid is known to cause allergies and mutagenic, while arbutinisis is known to have cytotoxic properties lower than hydroquinone, but less satisfactory depigmentation activity. There was a compound that has been synthesized by removing the hydroxy group of arbutin, known as deoxyarbutin (4-[Tetrahydro-2H-Pyrans-2-yl) oxy] phenol). Deoxyarbutin (dA) shows Ki 10-fold is lower than hydroquinone and 350-fold is lower than arbutin. IC50dA is 17.5+0.5 µmol/l, while the IC50 hydroquinone is 73.7+9.1 µmol/l. In terms of security, dA indicates that cell viability is 95% higher than hydroquinone. However, dA is thermolabile and photolabile. Several studies have shown satisfactory results to improve the stability of dA, that these compounds are considerable potential for further development as a depigmentation agent. The aim of this review is to describe how the potency of dA as a tyrosinase inhibitor interferes melanogenesis process through the latest depigmentation agent, its safety, efficacy and stability.

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Isolation of Arbutin from Leaves and Fruits of Buni (Antidesma Bunius L. Spreng) As Tyrosinase Enzym Inhibitor

Bioinformatics and Biomedical Research Journal ◽

10.11594/bbrj.04.01.02 ◽

2021 ◽

Vol 4 (1) ◽

pp. 8-18

Author(s):

Gea Ocktiah Palensina ◽

Leni Rosita ◽

Zuraida Sagala

Keyword(s):

Quantitative Analysis ◽

Mobile Phase ◽

Enzyme Inhibitor ◽

Qualitative And Quantitative Analysis ◽

Qualitative And Quantitative ◽

Extractive Substances ◽

Ic50 Values ◽

Rf Values ◽

Tyrosinase Enzyme

Several studies have shown that plant extractive substances have the potential as active compounds inhibiting the enzyme tyrosinase. Arbutin is an enzyme inhibitor of tyrosinase, known as a popular whitening agent used in cosmetics because of its effectiveness in overcoming skin hyperpigmentation. The purpose of this study was to conduct a qualitative and quantitative analysis of arbutin on Buni Leaves and Fruits (Antidesma bunius L. Spreng). The raw simplicia used are mature and young buni leaves, green, red and purple buni fruits. The extraction method is maceration using methanol as solvent. The initial screening for arbutin content was carried out using thin layer chromatography (TLC) and dichlormethan:methanol 50:50 used as mobile phase. Isolation of arbutin content was carried out using Preparative TLC with the same eluent. Qualitative and quantitative analysis were performed using High Pressured Liquid Chromatography (HPLC) with mobile phase of acetonitrile: water 60:40. The tyrosinase enzyme inhibition activity test was then carried out in vitro using 96-well microplate, l-tyrosine and l-dopa were used as substrate at a wavelength of 492 nm. The Rf values obtained ??for mature buni leaves and green buni fruits, respectively 0.61 and 0,62. The retention time of HPLC chromatogram respectively 2,784 minutes and 2,758 minutes. Arbutin levels in leaves and fruits are 7.9 mg / g and 2 mg / g. The activity of the enzyme tyrosinase of mature buni leaves on L-dopa and L-tyrosine substrate were respectively stated as IC50 values ??of 88.7191ppm and 101.33347 ppm. The activity of the enzyme tyrosinase of the green buni fruit on L-dopa and L-tyrosine substrate respectively stated IC50 values ??of 198,0293 ppm and 246,1296 ppm.

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