scholarly journals The effect of methyl jasmonate on ethylene production and CO2 evolution in Jonagold apples

2013 ◽  
Vol 48 (2) ◽  
pp. 121-128 ◽  
Author(s):  
Artur Miszczak ◽  
Edward Lange ◽  
Marian Saniewski ◽  
Janusz Czapski
Keyword(s):  
Methyl Jasmonate ◽  
Ethylene Production ◽  
Oxidase Activity ◽  
Acc Oxidase ◽  
Skin Area ◽  
Co2 Evolution ◽  
Time Of Application ◽  
Endogenous Ethylene ◽  
Metabolic Significance

Apples cv. Jonagold were harvested at the beginning of October and stored at 0°C until treatment between the beginning of December and the end of January. Methyl jasmonate (JA-Me) at the concentration of l,0, 0,5, 0,1, 0,05, and 0,01% in lanolin paste were applied to the surface ofintact apples. During five days from treatment, samples of cortex with skin (area about 2,0 cm<sup>2</sup>) were cut off at a depth of about 2 mm and used for determination of ethylene production, ACC oxidase activity and respiration determined as CO<sub>2</sub> evolution. The production of endogenous ethylene was highest at mid-January ( 100, 280, and 250 nl/g*h at December, mid-January, and the end of January, respectively). During December and at the beginning of January, JA-Me initially ( 1 -2 days after treatment) stimulated ethylene production and then the production was inhibited. The lower concentration of JA-Me caused initially the greater stimulation and then Iower inhibition of ethylene production. However, at the time of maximum production of endogenous ethylene (mid-January) and later. stimulatory effect of JA-Me disappeared. The effect of various concentrations and time of application of JA-Me on ACC oxidase activity had similar trend as endogenous ethylene production. Methyl jasmonate stimulated respiration and this effect was dependent on JA-Me concentration and independent on time of application. The metabolic significance of these findings is discussed.

scholarly journals The Effect of MethyI Jasmonate on Ethylene Production, ACC Oxidase Activity and Carbon Dioxide Evolution in the Yellowish-Tangerine Tomato Fruits (Lycopersicon esculentum Mill.)

2013 ◽  
Vol 49 (1-2) ◽  
pp. 67-72
Author(s):  
Janusz Czapski ◽  
Artur Miszczak ◽  
Marian Saniewski
Keyword(s):  
Carbon Dioxide ◽  
Ethylene Production ◽  
Oxidase Activity ◽  
Green Light ◽  
Acc Oxidase ◽  
Carbon Dioxide Production ◽  
Carbon Dioxide Evolution ◽  
Light Yellow ◽  
Endogenous Ethylene ◽  
Advanced Stages

The yellowish-tangerine tomato (cv. Bursztyn) in the green, light yellow and yellow stages of ripening were treated with 0.1% and 1.0% of methyl jasmonate (JA-Me) in lanolin paste and kept for several days and then they were evaluated for production of ethylene, ACC oxidase activity and CO<sub>2</sub> evolution. Production of endogenous ethylene in mature green fruits was low and increased during ripening. JA-Me stimulated ethylene production and ACC oxidase activity in all investigated stages of fruit ripening. Slices excised from mature green fruits produced highest amount of carbon dioxide as compared to more advanced stages of ripening. JA-Me in O,1 % and 1,0% concentrations increased significantly CO<sub>2</sub> evolution in green fruits, while in light yellow and yellow fruits only higher concentration of JA-Me stimulated carbon dioxide production.

scholarly journals 24-Epibrasinolide Modulates the Vase Life of Lisianthus Cut Flowers by Modulating ACC Oxidase Enzyme Activity and Physiological Responses

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 995
Author(s):  
Mohammad Darvish ◽  
Habib Shirzad ◽  
Mohammadreza Asghari ◽  
Parviz Noruzi ◽  
Abolfazl Alirezalu ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Water Absorption ◽  
Ethylene Production ◽  
Oxidase Activity ◽  
Acc Oxidase ◽  
Postharvest Quality ◽  
Vase Life ◽  
Dependent Manner ◽  
Cut Flowers ◽  
Oxidase Enzyme

Ethylene is the most important factor playing roles in senescence and deterioration of harvested crops including cut flowers. Brassinosteroids (BRs), as natural phytohormones, have been reported to differently modulate ethylene production and related senescence processes in different crops. This study was carried out to determine the effects of different levels of 24-epibrassinolide (EBL) on ACC oxidase enzyme activity, the final enzyme in ethylene biosynthesis pathway, vase life, and senescence rate in lisianthus cut flowers. Harvested flowers were treated with EBL (at 0, 3, 6, and 9 µmol/L) and kept at 25 °C for 15 days. The ACC oxidase activity, water absorption, malondialdehyde (MDA) production and vase solution absorption rates, chlorophyll and anthocyanin contents, and the vase life of the flowers were evaluated during and at the end of storage. EBL at 3 µmol/L significantly (p ≤ 0.01) enhanced the flower vase life by decreasing the ACC oxidase activity, MDA production and senescence rates, and enhancing chlorophyll and anthocyanin biosynthesis and accumulation, relative water content, and vase solution absorption rates. By increasing the concentration, EBL negatively affected the flower vase life and postharvest quality probably via enhancing the ACC oxidase enzyme activity and subsequent ethylene production. EBL at 6 and 9 µmol/L and in a concentration dependent manner, enhanced the ACC oxidase activity and MDA production rate and decreased chlorophyll and anthocyanin accumulation and water absorption rate. The results indicate that the effects of brassinosteroids on ethylene production and physiology of lisianthus cut flowers is highly dose dependent.

Harvest maturity related changes in the cold-induced activation of 1-aminocyclopropane-1-carboxylic acid metabolism in Granny Smith apples / Efecto del estado de madurez sobre la activación por frío del metabolismo del ácido 1-aminociclopropano-1-carboxílico en manzanas Granny Smith

1999 ◽  
Vol 5 (3) ◽  
pp. 223-228 ◽  
Author(s):  
C. Larrigaudiere ◽  
I. Recasens ◽  
J. Graell ◽  
M. Vendrell
Keyword(s):  
Carboxylic Acid ◽  
Ethylene Production ◽  
Oxidase Activity ◽  
Acid Metabolism ◽  
Cold Treatment ◽  
Acc Oxidase ◽  
Malus Domestica Borkh ◽  
Harvest Dates ◽  
Harmonization Process ◽  
Harvest Maturity

Changes in 1-aminocyclopropane-1-carboxylic acid metabolism in apples ( Malus domestica Borkh cv Granny Smith) were studied in relation to cold storage. Emphasis was given to the differential re sponsiveness of fruits to cold treatment as a function of stage of maturity at harvest. Fruits were held at 1 or 20 °C for 30 days, respectively, or exposed to 1 °C for 10 days and then storaged at 20 °C for up to 30 days. Fruits at 20 °C showed typical climacteric behavior. Differences at 1 °C between maturity stages in ethylene production and ACC oxidase activity were abolished, which showed that cold treatment is an important inducer of climacteric rise in preclimacteric Granny Smith apples. At 1 °C, ethylene production was lower than at 20 °C and the maxima in production were similar for all the stages of maturity, but took place at different times which corresponded exactly to the initial differ ences in harvest dates. After the transfer to 20 °C, fruits exhibited similar behavior as regards ethyl ene production, ACC oxidase activity, and ACC and MACC levels in relation to a harmonization process which is discussed in this study.

Regulation of ethylene production and ripening by saskatoon (Amelanchier alnifolia Nutt.) fruit

1998 ◽  
Vol 76 (10) ◽  
pp. 1743-1754
Author(s):  
Suzy Y Rogiers ◽  
GN Mohan Kumar ◽  
N Richard Knowles
Keyword(s):  
Ethylene Production ◽  
Acc Oxidase ◽  
Fold Increase ◽  
Dry Weight ◽  
Oxidase Inhibitor ◽  
Aminooxyacetic Acid ◽  
Acid Oxidase ◽  
Amelanchier Alnifolia ◽  
Endogenous Ethylene ◽  
Maturity Class

Changes in respiration and ethylene production were characterized during maturation and ripening of saskatoon (Amelanchier alnifolia Nutt.) fruit. On a per fruit basis, respiration and ethylene production increased 78 and 400%, respectively, as fruit ripened on the plant and trends were consistent with those for climacteric fruits. When estimated on a fresh and dry weight basis, increased rates of ethylene production were still apparent during ripening; however, respiration rate declined. Trends in respiration rates and endogenous ethylene levels of harvested fruit of nine maturity classes, from immature green (class one) to fully ripe and purple (class nine), were consistent with those of fruit growing on the plant. Tissue prints showed that ACC (1-aminocyclopropane-1-carboxylic acid) oxidase was distributed throughout the pericarp of fruit at all nine maturity stages and that the enzyme was most concentrated in the immature stages on a per fruit basis. On a protein basis, ACC oxidase increased progressively with development of cv. Smoky fruit but remained relatively constant over the nine maturity classes of cv. Northline fruit. In contrast, ACC oxidase levels were relatively low in cv. Pembina fruit over the first four maturity classes, increased substantially as fruits developed from class four to five, then remained constant as fruit ripened to maturity class nine. Treating immature harvested Pembina fruit (maturity classes one to three) with ACC effected a 28- to 108-fold increase in ethylene production, compared with an average of only 7-fold for ACC-treated fruit of maturity classes four through nine. Preharvest treatment of class-three fruit with ACC induced ripening to maturity class eight within 5 days, while untreated fruit required 15 days to reach class eight. Vacuum infiltrating class four fruit with alpha-aminooxyacetic acid (AOA) or aminoethoxyvinyl glycine (AVG) inhibited ethylene production and color development substantially. The inhibiting effect of AOA on ripening was eliminated when fruit were infiltrated with equimolar concentrations of AOA and ACC. Cobalt, an ACC oxidase inhibitor, also inhibited ethylene production and ripening. Collectively, our results indicate that ethylene synthesis by preclimacteric fruit is limited by the availability of ACC, ethylene is responsible for initiating ripening, and thus, saskatoon fruit are climacteric.Key words: Amelanchier alnifolia, saskatoon, fruit ripening, ethylene.

scholarly journals Postharvest Behavior of Transgenic Cantaloupe Melons with Suppressed ACC Oxidase Activity

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 571d-571
Author(s):  
Jean-Claude Pech
Keyword(s):  
Cell Wall ◽  
Ethylene Production ◽  
Biochemical Composition ◽  
Oxidase Activity ◽  
Acc Oxidase ◽  
Cell Wall Degradation ◽  
Antisense Gene ◽  
Ripening Process ◽  
Respiratory Behavior ◽  
Exogenous Ethylene

We have generated transgenic Cantaloupe Charentais melons expressing an ACC oxidase antisense gene in which ethylene production was reduced to less than 1% as compared to control untransformed fruits. As a consequence, some aspects of the ripening process were strongly inhibited (aroma volatiles production, chlorophyll and cell wall degradation, pigmentation of the rind, activation of peduncular abscission zone) while others remained unchanged (coloration of the flesh), allowing us to distinguish between ethylene-dependent and ethylene-independent pathways. Some postharvest characteristics of the transgenic fruit are described in terms of expression of ripening-related genes, respiratory behavior, and biochemical composition. Data also are presented showing that exogenous ethylene treatments could reverse the antisense phenotype.

scholarly journals Heat treatment effects on ACC oxidase activity of 'Keitt' mangoes

2003 ◽  
Vol 15 (3) ◽  
pp. 145-148 ◽  
Author(s):  
Renar João Bender ◽  
Eduardo Seibert ◽  
Jeffrey K. Brecht
Keyword(s):  
Ethylene Production ◽  
Oxidase Activity ◽  
Acc Oxidase ◽  
Hot Water ◽  
In Vitro Activity ◽  
Ethylene Dibromide ◽  
Heat Treated ◽  
Physical Treatments ◽  
Water Treatments

With the use of ethylene dibromide for mango disinfestation being ruled out, vapor heat or hot water treatments are the only alternatives for quarantine treatments of mangoes. Physical treatments such as heat treatments have been implicated in higher incidence of physiological disorders and enhancement of ripening processes. Therefore, the objective of the present work was to determine the effects of hot water treatments on ethylene production and on the in vitro activity of ACC oxidase. Cv. Keitt mangoes were immersed for 3 min in hot water at 53 °C or 90 min in water at 46 °C. Immediately after the treatments, some of the mangoes were analyzed for ACC oxidase activity and others were stored to be analyzed after 4 days at 12 °C. There was a significant increase in the ACC oxidase activity just after the hot water treatments. After 4 days, only the mangoes treated for 90 min maintained high ethylene production and ACC oxidase activity. Tissue from the outer layers of the mesocarp had higher enzyme activity compared to tissues from the innermost layers of the mesocarp of heat-treated mangoes.

Effects of Methyl Jasmonate on Anthocyanin Accumulation, Ethylene Production, and CO2 Evolution in Uncooled and Cooled Tulip Bulbs

1998 ◽  
Vol 17 (1) ◽  
pp. 33-37 ◽  
Author(s):  
M. Saniewski ◽  
A. Miszczak ◽  
L. Kawa-Miszczak ◽  
E. Wegrzynowicz-Lesiak ◽  
K. Miyamoto ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Ethylene Production ◽  
Co2 Evolution ◽  
Anthocyanin Accumulation ◽  
Tulip Bulbs

Regulation of ethylene and polyamine synthesis by elevated carbon dioxide in cherimoya fruit stored at ripening and chilling temperatures

1999 ◽  
Vol 26 (3) ◽  
pp. 201 ◽  
Author(s):  
M. T. Muñoz ◽  
P. Aguado ◽  
N. Ortega ◽  
M. I. Escribano ◽  
C. Merodio
Keyword(s):  
Ethylene Production ◽  
Oxidase Activity ◽  
Acc Oxidase ◽  
Arginine Decarboxylase ◽  
Chilling Temperature ◽  
Polyamine Biosynthesis ◽  
Polyamine Synthesis ◽  
High Co2 ◽  
Chilling Temperatures ◽  
Co2 Level

In this study we focused on the effect of high CO2 level (20%) on ethylene and polyamine biosynthesis in cherimoya (Annona cherimola Mill.) fruits stored at ripening (20˚C) and chilling (6˚C) temperatures. At ripening temperature, CO2 inhibited ethylene production, but 1-aminocyclopropane-1- carboxylate (ACC) oxidase activity was similar to that in ripe control fruits. CO2 treatment led to a decline in putrescine (Put) and a major accumulation of spermidine (Spd) and spermine (Spm) without any effect on arginine decarboxylase (ADC) activity. These results confirm the preferential transformation of Put to Spd and Spm in CO2 -treated fruits. At chilling temperature, the increase in ACC oxidase activity was inhibited and the Vmax of ADC increased. A combination of chilling temperature storage and high CO2 level led to suppression of basal ethylene production while ACC oxidase activity remained unchanged. In addition, fruits held at these conditions had higher polyamine titres than the untreated control. We propose that, in CO2 -treated fruits, the absence of autocatalytic or basal ethylene production, depending on the temperature, may be due to deviation of the S-adenosylmethionine (SAM) pool towards polyamine synthesis, primarily Spd and Spm.

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