scholarly journals Effect of growth retardants, cytokinins and auxins on the multiplication and rooting in vitro of Alstroemeria x hybrida "Juanita"

2013 ◽  
Vol 51 (1-2) ◽  
pp. 23-31 ◽  
Author(s):  
Małgorzata Podwyszyńska ◽  
Eleonora Gabryszewska ◽  
Andrzej Przybyła
Keyword(s):  
Shoot Formation ◽  
Naphthaleneacetic Acid ◽  
Growth Retardants ◽  
Rooting Ability ◽  
Aerial Shoot ◽  
Low Concentrations ◽  
Shoot Production ◽  
Indole 3 Acetic Acid ◽  
New Cultivars

Rhizome cultures of "Jiianita" Polish cultivar of Alstroemeria x hybrida were used to enhance an effectiveness of micropropagation method of new cultivars and selections. The effect of cytokinins (BAP. kinetin and 2iP), auxins (IAA, IBAand NAA), growth retardants (paclobutrazol and flurprimidol alone or in combination were studied in relation to rhizome branching. aerial shoot production and rooting of rhizome. The greatest number of aerial shoots as well as the shortest shoots were observed at the highest BAP concentration (6 mg l<sup>-1</sup>). However, the rhizonies had the poorest rooting ability. BAP at low concentrations combined with kinetin or 2iP also strongly stimulated aerial shoot formation and rhizome branching. Unfortunately. those shoots were of poor qualily. Application of BAP at low concentration with paclobutrazol (0,1-0,5 mg l<sup>-1</sup> ) or flurprimidol (0,01- 1 mg l<sup>-1</sup>) in presence of 1 mg l<sup>-1</sup> NAA resulted in high number of aerial shoots (5-6), reduction of their length and higher rooting ability of the rhizomes. Gr()wth retardants applied with NAA strongly stimulated formation of the roots but suppressed their elongation. Abbreviations: BAP - 6=benzylaminopurine; kinetin - 6-furfurylaminopurine; 2iP - 6-‌γ,γ-dime-thylallylamino]purine); IAA-indole-3-acetic acid; IBA-indole-3-butyric acid; NAA- naphthaleneacetic acid; paclobutrazol (ICI PP-333) - (2-RS,3-RS)-1-(4-chlorophenyl)-4-4-dimethyl-2(1,2,3-triazol-1-yl)-pentan-3-ol flurprimidol (Dowelanco) - α-(1-niethylethy 1-α-[4-trifluro-niethoxy)phenyl]-5-pyridinemethanol.

scholarly journals Propagation and Establishment of Three Endangered Mexican Orchids from Protocorms

HortScience ◽  
2009 ◽  
Vol 44 (5) ◽  
pp. 1395-1399 ◽  
Author(s):  
Martín Mata-Rosas ◽  
Víctor M. Salazar-Rojas
Keyword(s):  
In Vitro Propagation ◽  
Culture Media ◽  
Survival Rates ◽  
Basal Medium ◽  
Shoot Formation ◽  
Naphthaleneacetic Acid ◽  
Ex Vitro ◽  
Shoot Production ◽  
Further Development

Protocols for in vitro propagation from protocorms of Mormodes tuxtlensis Salazar, Cuitlauzina pendula La Llave & Lex., and Lycaste skinneri (Batem. Ex. Lind.) Lind., three endangered species distributed in Mexico and highly appreciated as ornamentals, were developed. The effect of two different culture media, Murashige and Skoog (MS) and modified Knudson (KCm), combined with varying concentrations of N6-benzyladenine (0, 2.2, 4.4, 8.9, and 13.3 μM) and α-naphthaleneacetic acid (0, 0.5 and 2.7 μM), were investigated. Shoot formation and development of protocorm-like bodies were observed. For all three species, cultures in MS produced more shoots per explant than those in KCm, and those shoots were longer and more robust in appearance. Maximum number of shoots for M. tuxtlensis (1.5) and C. pendula (24.3) were obtained in media supplemented with 13.3 μM and 2.2 μM N6-benzyladenine, respectively. Conversely, for L. skinneri the greatest shoot production (16.4) was achieved in medium supplemented with 2.7 μM α-naphthaleneacetic acid. Subculturing explants in MS basal medium allowed further development and rooting of the shoots as well as growth of protocorm-like bodies. The effect of different potting mixes on ex vitro survival plantlets was also investigated; pine bark:oak charcoal:pumice (3:1:1) allowed the highest survival rates in all three species.

scholarly journals Micropropagation of Lupinus texensis from Cotyledonary Node Explants

HortScience ◽  
1992 ◽  
Vol 27 (11) ◽  
pp. 1222-1223 ◽  
Author(s):  
Abba Upadhyaya ◽  
Tim D. Davis ◽  
Daksha Sankhla ◽  
N. Sankhla
Keyword(s):  
Adventitious Root ◽  
Root Formation ◽  
Low Frequency ◽  
Cotyledonary Node ◽  
Shoot Formation ◽  
Adventitious Root Formation ◽  
Naphthaleneacetic Acid ◽  
Hypocotyl Explants ◽  
Indole 3 Acetic Acid

Both kinetin and BA promoted in vitro shoot formation from hypocotyl explants of Lupinus texensis Hook. placed on Murashige and Skoog (MS) medium. With either cytokinin, shoot formation was best at ≈4.5 μm. Adventitious root formation was observed only on tissue culture-derived shoots placed in MS media containing 5.4 to 54 μM NAA. IAA and IBA, at concentrations ranging from 5 to 55 μm, failed to stimulate rooting. Even at the optimal concentration of NAA, only 14% of the shoots produced roots. Thus, although hypocotyl explants readily produced shoots, adventitious root formation on these shoots occurred with relatively low frequency. Chemical names used: 6-benzylaminopnrine (BA); indole-3-acetic acid (IAA); indole-3-butyric acid (IBA); 6-furfurylaminopurine (kinetin); 1-naphthaleneacetic acid (NAA).

Effect of two bio-polysaccharides on organogenesis of protocorm-like bodies in Phalaenopsis cultured in vitro

2020 ◽  
Vol 26 (1) ◽  
pp. 2137-2142
Author(s):  
A. M. Meskatul ◽  
K. Shimasaki ◽  
S. U. Habiba
Keyword(s):  
Shoot Formation ◽  
Vital Role ◽  
White Led ◽  
Fresh Weight ◽  
High Concentration ◽  
Low Concentrations ◽  
Different Types ◽  
Modified Ms Medium ◽  
Led Lights

Different types of bio-polysaccharide play a vital role in the growth of PLBs cultured in vitro. In this study, to we investigated the potential impacts of two bio-polymers,: hyaluronic acid (HA9) and sodium alginate (ALG) on the organogenesis of protocorm-like bodies (PLBs) in Phalaenopsis under white LED lights. PLBs of Phalaenopsis ‘Fmk02010’ were explanted on modified MS medium with different concentrations of HA and (ALG). The highest average number of PLBs per explant (24.6) was recorded for ALG alone at a concentration of 0.01mg/L, and the fresh weight was also highest at the same concentration. The combination of 0.01mg/L ALG and 0.01mg/L HA also resulted in a large number of PLBs (23.8) and high fresh weight. As opposed to, the highest number of shoots /explant (3.6) was observed at the treatment of the combination of 1mg/L ALG and 10mg/L HA. This study shows that the application of ALG and HA alone, and in combination, at low concentrations, increased the average number of PLBs and the amount of fresh weight, but shoot formation was higher at a high concentration compared with control.

scholarly journals INCREASED SHOOT PROLIFERATION OF APPLES CO-CULTIVATED WITH AGROBACTERIUM TUMEFACIENS

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 661a-661
Author(s):  
F.A. Hammerschlag ◽  
R.H. Zimmerman ◽  
A.C. Smigocki
Keyword(s):  
Shoot Proliferation ◽  
Shoot Formation ◽  
Pcr Analysis ◽  
Axillary Shoot ◽  
Putative Transformants ◽  
Proliferation Medium ◽  
Shoot Production ◽  
Dna Fragment ◽  
Different Levels

`McIntosh' apple shoots were inoculated in vitro with Agrobacterium tumefaciens strain tms328::Tn5 (tms) carrying a functional cytokinin gene. Callus tissue, removed from the infected stems, produced shoots on shoot proliferation medium. After three subcultures, axillary shoot production from a tms-infected putative transformant was eight times that of controls. Subsequent shoot production on three different levels of BA (3, 6 and 10 uM) was significantly greater than from controls on all levels of BA. PCR analysis of putative transformants revealed an expected 503 bp DNA fragment corresponding to the amplified portion of the cytokinin gene. After 6 months of in vitro propagation, proliferation rates of shoots obtained from the original transformants were similar to the controls and the expected PCR fragment of 503 bp could only be detected by Southern analysis. Even though the T-DNA appears to be lost from the apple genome, the data suggest that the tms strain may be useful in co-infection experiments to induce shoot formation, thus avoiding difficult regeneration procedures.

scholarly journals Isolated culture of A. reptance L., its’ morphological and growth features

2021 ◽  
Vol 40 ◽  
pp. 01001
Author(s):  
Elen Poghosyan ◽  
Naira Sahakyan ◽  
Margarit Petrosyan ◽  
Irina Batlutskaya ◽  
Karen Trchounian
Keyword(s):  
Callus Culture ◽  
Nutrient Medium ◽  
Leaf Explants ◽  
Shoot Formation ◽  
Callus Cultures ◽  
Soil Conditions ◽  
Naphthaleneacetic Acid ◽  
Micro Propagation ◽  
2,4 Dichlorophenoxyacetic Acid

A growing demand for the ecologically pure products brings us for searching novel biotechnological approaches for plant cultivation. One of these approaches is the in vitro cultivation and further acclimatization of valuable plant species. The object of our investigation was Ajugareptance L. ornamental plant which possesses high metabolic activity. In vitro cultivation was carried out applying Murashige-Skoog nutrient medium and its modifications. Acclimatization of in vitro plants was implemented according Hazarika. In the presence of twice higher concentration of cytokinins over auxins and 0.2 mg/ml gibberellins callus culture was formed from the leaf explants. Callus tissue was formed in the presence of 0.2 mg/ml kinetin and 2 mg/ml indole-3-acetic acid which has denser structure than the first one. The shoot formation was observed on callus cultures growing on the same medium approximately after 5th passage. Callus culture growth was supported also by the adding of 2 mg/ml 2,4-dichlorophenoxyacetic acid. For the micropropagation, the already formed shoots were transferred to the nutrient medium which contains only 0.1 mg/ml 1-Naphthaleneacetic acid as a phytohormone. A. reptans culture has high regenerative ability and the micro-propagation index was 104 – 105. In vitro regenerated plants were successfully acclimatized to the soil conditions during two-week period.

scholarly journals Pomegranate Micropropagation, Callogenesis and Genetic Integrity Assessment Using Simple Sequence Repeat Markers

2018 ◽  
Vol 11 (1) ◽  
pp. 237
Author(s):  
Tebogo Stimela ◽  
Remmy W. Kasili ◽  
Edward G. Mamati
Keyword(s):  
Acetic Acid ◽  
Simple Sequence Repeat ◽  
Naphthaleneacetic Acid ◽  
Genetic Integrity ◽  
Sequence Repeat ◽  
Simple Sequence Repeat Markers ◽  
Half Strength ◽  
Indole 3 Acetic Acid ◽  
Simple Sequence

In recent years, the awareness of pomegranate health benefits has grown exponentially; nonetheless the existing propagation methods remain a challenge to supply adequate suitable planting materials needed for commercial production. Micropropagation can lead to mass production of plantlets and callus-mediated in vitro regeneration can open avenues for the use of genetic engineering to improve this crop. The aim of this study was to evaluate appropriate conditions for pomegranate micropropagation, callogenesis and use Simple Sequence Repeat markers to screen for somaclonal variation. Cytokinins (Benzylaminopurine, Kinetin and Thiadiazol-5ylurea) were tested for shoot induction from nodal explants while auxins (1-Naphthaleneacetic acid, Indole-3-butyric acid and Indole-3-acetic acid) were tested for root induction of in vitro regenerated shoots. 1-Naphthaleneacetic acid combined with Benzylaminopurine was assessed for their ability to induce callus from cotyledon and leaf explants. Genetic integrity between mother plant, callus and in vitro regenerated shoots were assessed using eight Simple Sequence Repeat markers. Maximum number of shoots and leaves were obtained on full strength Murashige and Skoog media with 6.9 &micro;M kinetin. The highest number of roots was achieved on half strength Murashige and Skoog media with 4.9 &micro;M Indole-3-butyric acid and the longest root was got on half strength Murashige and Skoog media with 5.3 &micro;M Indole-3-acetic acid. Leaves and cotyledons demonstrated to be potential explants for callus formation at all hormonal combination levels tested. Eight out of 13 amplified alleles were polymorphic. A wider genetic variation was found with similarity coefficient range of 0.46-0.92. More somaclonal variation was in regenerated shoots compared to callus.

scholarly journals Development of an Efficient Plant Regeneration System for the Selenium-hyperaccumulator Astragalus racemosus and the Nonaccumulator Astragalus canadensis

HortScience ◽  
2008 ◽  
Vol 43 (7) ◽  
pp. 2138-2142 ◽  
Author(s):  
Chiu-Yueh Hung ◽  
Jiahua Xie
Keyword(s):  
Plant Regeneration ◽  
Basal Medium ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Naphthaleneacetic Acid ◽  
Shoot Induction ◽  
Regeneration System ◽  
Rooting Medium ◽  
Significant Difference

A method of in vitro plant regeneration for both the selenium-hyperaccumulator Astragalus racemosus ‘Cream Milkvetch’ and the nonaccumulator Astragalus canadensis ‘Canadian Milkvetch’ was developed with two induction media, M1 and M2. The M1 and M2 contain Murashige and Skoog basal medium plus vitamins, 8.07 μm N-(2-chloro-4-pyridyl)-N′-phenylurea, 2.5% (w·v−1) sucrose, 0.7% (w·v−1) agar (pH 5.7), and 0.89 μm or 3.12 μm a-naphthaleneacetic acid, respectively. In vitro cultures were initiated on these two types of media with three types of explants: cotyledons, hypocotyls, and roots. More than 93% of cultured explants from both species could form calli or calli with shoots. With regard to shoot formation, A. canadensis could produce multiple shoots from all types of explants more efficiently than A. racemosus. The highest shoot induction was approximately three shoots per explant in A. racemosus, whereas A. canadensis could reach ≈10 shoots per explant. M1 could induce more shoots than M2 no matter what type of explant was used, but the overall induction rates were no significant difference. Among the three types of explants used, the cotyledons were the best explants for shoot induction in A. canadensis, whereas hypocotyls were the best in A. racemosus. In A. racemosus, shoots could also be obtained from calli on the rooting medium containing Murashige and Skoog basal plus vitamins, 2.84 μm indole-3 acetic acid, 2.5% (w·v−1) sucrose, and 0.7% (w·v−1) agar (pH 5.7). Approximately 43% of A. canadensis shoots and 19% of A. racemosus shoots could be rooted on the rooting medium.

scholarly journals Multiple Shoot Production In Vitro of the Tropical Timber Tree, Sentang (Azadirachta excelsa Linn.)

HortScience ◽  
1998 ◽  
Vol 33 (6) ◽  
pp. 1073-1075 ◽  
Author(s):  
T.K. Liew ◽  
C.K.H. Teo
Keyword(s):  
Sodium Hypochlorite ◽  
Multiple Shoot ◽  
Axillary Buds ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Tropical Timber ◽  
Shoot Production ◽  
Timber Tree

Axillary buds from 5-month-old seedlings of Azadirachta excelsa Linn. were surface-sterilized twice with 1.35% (m/v) and 1.05% (m/v) of sodium hypochlorite for 25 and 15 minutes, respectively, before culturing on Murashige and Skoog (MS) medium containing combinations of BA and NAA. A combination of 4.4 μM BA + 0.5 μM NAA induced the most axillary buds to grow (eight per explant). Subsequent proliferation of the micropropagated shoots on this medium yielded abnormal shoots. The best medium for maximum proliferation of these micropropagated shoots contained 3.3 μM BA and 0.27 μM NAA. On this medium about four normal shoots were produced per explant. These findings indicate that two different media are needed for successful micropropagation of sentang. Chemical names used: N6-benzylaminopurine (BA); 1-naphthaleneacetic acid (NAA).

In Vitro Regeneration of Valencia-type Peanut (Arachis hypogaea L.) from Cultured Petiolules, Epicotyl Sections and Other Seedling Explants1

1992 ◽  
Vol 19 (2) ◽  
pp. 82-87 ◽  
Author(s):  
Ming Cheng ◽  
David C. H. Hsi ◽  
Gregory C. Phillips
Keyword(s):  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Direct Organogenesis ◽  
Naphthaleneacetic Acid ◽  
Regeneration System ◽  
Arachis Hypogaea L ◽  
Wide Range ◽  
Cultivated Peanut

Abstract This study evaluated plant development via direct organogenesis from in vitro-cultured young seedling tissues of cultivated peanut, especially the valencia-type peanut. Complete plants were regenerated from in vitro-cultured petiolule-with-blade-attached explants, leaflet segments, and epicotyl andpetiole sections. Multiple shoots arose on Murashige and Skoog medium (MS) supplemented with 6-benzylaminopurine (BA) (5–25 mg/L) plus 1-naphthaleneacetic acid (NAA) (0.5–3 mg/L). After 30 d culture on 25 mg/L BA + 1 mg/L NAA, 1.6 buds or shoots/explant were regenerated from the petiolule-with-blade-attached explants. Comparable numbers of shoots were obtained from epicotyl sections of the first node region of the seedling after 60 d culture using 10 mg/L BA + 1 mg/L NAA. Leaflet segments and petiole sections were less responsive for shoot formation. Excised shoots developed roots in vitro upon transfer for 15 d to MS medium supplemented with NAA at 1 mg/L. Plantlets were transferred to soil and grown in a greenhouse to maturity. A wide range of cultivated peanut genotypes was evaluated for organogenic responsiveness, using the petiolule-with-blade-attached explant source. Only valencia-type cultivars, or a hybrid derivative with a Valencia background, were responsive with this regeneration system.

scholarly journals Efficient In Vitro Plant Regeneration from Internode Explants of Ibervillea sonorae: An Antidiabetic Medicinal Plant

HortScience ◽  
2017 ◽  
Vol 52 (7) ◽  
pp. 1000-1005 ◽  
Author(s):  
Ilse-Yazmín Arciniega-Carreón ◽  
Carmen Oliver-Salvador ◽  
María-Guadalupe Ramírez-Sotelo ◽  
Carlos Edmundo Salas
Keyword(s):  
Acetic Acid ◽  
Plant Regeneration ◽  
Medicinal Plant ◽  
Metabolic Disorders ◽  
Naphthalene Acetic Acid ◽  
In Vitro Plant Regeneration ◽  
Shoot Production ◽  
In Vitro Plant ◽  
Indole 3 Acetic Acid

Ibervillea sonorae is a medicinal plant mainly used to treat diabetes, ulcers, and other metabolic disorders. A regeneration protocol using internode segments containing axillary buds grown on Gamborg medium (B5) supplemented with 0.5 mg·L−1 α-naphthalene-acetic acid (NAA), 0.5 mg·L−1 N6-benzyladenine (BA), and 1.0 mg·L−1 indole-3-acetic acid (IAA) successfully regenerated shoots in I. sonorae explants. The induction of organogenic calli attained 100% efficiency. The highest percent shoot production was 87.5% with a mean of 9.17 shoots per explant on day 15, and the maximum length of 5.8 cm was observed on day 21. Regenerated shoots induced roots in B5 medium supplemented with 0.5–3.0 mg·L−1 indole-3-butyric acid (IBA). The maximum rooting frequency observed in the medium containing 2.0 mg·L−1 IBA was 83.3% which promoted long, thick roots on day 21. The plantlets with emerging roots grown at the culture facility attained 50% survival after acclimatization for 30 d. The account describes a simple and efficient protocol for in vitro plant regeneration, and this micropropagation procedure offers an alternative for preservation of this medicinal plant.

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