scholarly journals Formation of the phenotypic structure of Phytophthora infestans population in Poland during 1987-2001

2013 ◽  
Vol 55 (1) ◽  
pp. 389-400 ◽  
Author(s):  
Hanna Zarzycka ◽  
Sylwester Sobkowiak ◽  
Renata Lebecka ◽  
Beata Tatarowska

The formation of phenotypic structure of <i>P.infestans</i> population in Poland was determined by analyzing 1603 isolates collected from 1987 to 2001. The race complexity, low at the beginning of experiment, has been increasing from year to year and reached in 2001 a high level 7,2 virulence factors per isolate. The single and less composed races dominating firstly in the population were replaced gradually by more composed races. The virulence factors 1, 2, 3, 4, 7 and 11 occurred most frequently, but factors 5 and 8 were noted sporadically. The A2 mating type was detected in 1988 at first and since that time its occurrence has been noted in Polish population each year. The oospores were formed in potato tissues. Race diversity, low at the beginning of the investigation, reached a peak in 1996-2001 . During 1987-1990 weakly and middly aggressive phenotypes dominated in Polish population. In the later period very aggressive isolates were more frequent. Phenotypic race similarity of <i>P.infestans</i> populations in 1987 and 2001 was very low. The race structure of 1987 population was totally different from the race structure of populations of the next years. It was probably due to migration of new pathotypes. On the other hand the variation in complexity, diversity and similarity of races, as well as in aggressiveness observed in later years of investigation can be caused by the presence of both mating types and sexual recombination.


Plant Disease ◽  
2007 ◽  
Vol 91 (1) ◽  
pp. 109-109 ◽  
Author(s):  
J. M. Segura ◽  
M. de Cara ◽  
M. Santos ◽  
J. Tello

During 2004, an unusual spread of Phytophthora infestans on tomato plants in greenhouses located in Almería and Granada provinces, southern Spain, was observed. Infected plants had water-soaked, brown spots on leaves and stems and necrotic areas with white mold on the surface of fruits. Three isolates were obtained by plating diseased tissue on V8 juice agar medium and maintained on rye agar at 18°C. These isolates were analyzed for the mating type. Crosses were carried out using V8 juice agar and rye agar. The two parental isolates US1 (A1) and US8 (A2) were both provided by W. E. Fry, Cornell University, Ithaca, NY. Two of the Spanish isolates were homothallic and the other isolate belonged to the uncommon mating type A1A2. To confirm the occurrence of the two mating types, 43 single-sporangium progeny were produced and analyzed from the A1A2 mating type. Thirty eight isolates were A1, two were A2, one was A1A2 mating type, and two were sterile. Assessment of five single-sporangium progeny from the homothallic type resulted in two A1, two homothallic, and one sterile isolate. A1A2 isolates produced oospores when crossed with either A1 or A2, but not when self-crossed. Previously, the A1A2 mating type has been found in Israel in the field and was obtained from oospores produced on tomato seeds (2,3). Since 2003, mating types of P. infestans isolates recovered from potato (60) and tomato (8) in southern Spain have been characterized. Seventy-five percent of the isolates recovered from potato were A1 and 25% were A2 mating types. Isolates recovered from tomato were 50% A1 and 50% A2 (1). To our knowledge, this is the first report of the occurrence of the A1A2 mating type and homothallic P. infestans isolates on tomato in Spain. References: (1) E. Andujar et al. Congr. Sociedad Española de Fitopatol. 12:244, 2004. (2) E. Rubin and Y. Cohen. Phytoparasitica 32:237, 2004. (3) E. Rubin and Y. Cohen. Plant Dis. 90:741, 2006.



Al-MAJAALIS ◽  
2018 ◽  
Vol 6 (1) ◽  
pp. 1-36
Author(s):  
Muhammad Arifin Badri

This study aims to examine the laws of dowry money decoration that are common in the community. The innovation and soul of art that is channeled through décor of dowry money is proven to produce beautiful and unique works, so as to attract the attention and interest of the wider community. However, because to produce beautiful and unique works, a high level of creativity is needed, so not everyone can do it. On the one hand, this phenomenon opens up quite good business opportunities, but on the other hand, it should be watched out, because in some conditions it contains the practice of buying and selling currencies with nominal differences. Through this study, I would like to uncover the law of buying and selling practices decorating dowry money and decorating services. As I also intend to present an applicative solution for the community so that they can still channel their artistic talents without violating Shari’ah law.



Genetics ◽  
1995 ◽  
Vol 141 (2) ◽  
pp. 503-512 ◽  
Author(s):  
H S Judelson ◽  
L J Spielman ◽  
R C Shattock

Abstract DNA markers linked to the determinants of mating type in the oomycete, Phytophthora infestans, were identified and used to address the genetic basis of heterothallism in the normally diploid fungus. Thirteen loci linked to the A1 and A2 mating types were initially identified by bulked segregant analysis using random amplified polymorphic DNA markers (RAPDs) and subsequently scored in three crosses polymorphisms (SSCP), cleaved amplified polymorphisms (CAPS), or allele-specific polymerase chain reaction markers (AS-PCR). All DNA markers mapped to a single region, consistent with a single locus determining both mating types. Long-range restriction mapping also demonstrated the linkage of the markers to one region and delimited the mating type locus to a 100-kb region. The interval containing the mating type locus displayed non-Mendelian segregation as only two of the four expected genotypes were detected in progeny. This is consistent with a system of balance lethal loci near the mating type locus. A model for mating type determination is presented in which the balanced lethals exclude form progeny those with potentially conflicting combinations of mating type alleles, such as those simultaneously expressing A1 and A2 functions.



2010 ◽  
Vol 42 (No. 2) ◽  
pp. 41-48 ◽  
Author(s):  
J. Mazáková ◽  
V. Táborský ◽  
M. Zouhar ◽  
P. Ryšánek ◽  
E. Hausvater ◽  
...  

A total of 199 <i>Phytophthora infestans</i> isolates were obtained from leaves, tubers and fruits of infected crops of potato and tomato in different regions of the Czech Republic in 2003, 2004 and 2005. They were analysed for mating type using the conventional pairing assay and PCR markers; 107 isolates were of A1 and 92 of A2 mating type. No self-fertile isolate was found. Our study is the first report of the presence and distribution of the A2 mating type of <i>P. infestans</i> in the Czech Republic. The co-existence of the two mating types may enable the pathogen to reproduce sexually, thus enhancing the diversity of its population countrywide.



1891 ◽  
Vol 37 (156) ◽  
pp. 179-183
Author(s):  
J. R. Gasquet

The chief feature in the psychological literature of Italy since my last report has been the multiplication of periodicals devoted to it. Thus we have received copies of the following new journals:—“The Annali di Freniatria,” of Turin; “Il Manicomio,” of Nocera Inferiore; “Il Pisani,” of Palermo; and “L'Anomalo,” a “Gazzettino,” published at Naples. On the other hand, I regret to say that no copies have been sent since 1888 of Lombroso's always interesting, if sometimes paradoxical, “Archivio di Psichiatria ed Antropologia Criminale.”To some extent these periodicals may be considered as equivalent to our Asylum Reports; and they doubtless show the great activity and zeal with which our specialty is studied in Italy. But from another point of view they seem to me no unmixed advantage. Each periodical is starved by the scattering of so much ability, which was formerly concentrated in fewer journals; and the space left vacant has been, to a great extent; occupied by matters of local, temporary, or secondary interest. When allowance is made for this, Italian psychological literature will be found quite equal to the high level it has attained in former years.



1955 ◽  
Vol 1955 ◽  
pp. 38-44 ◽  
Author(s):  
W. Thomson ◽  
I. McDonald

It is now a well established fact that high-level nutrition of the in-lamb ewe during the later stages of pregnancy results in heavy lambs of high vitality at birth and in good milking ewes. On the other hand, when ewes are poorly fed during the same period, or suffer a check, they produce underweight lambs of low vitality and have poor milk yields. While it is agreed that underweight lambs are undesirable, one is often asked whether the larger and sturdier lambs at birth maintain their superiority in later life, a problem on which relatively few results have been published. A study has therefore been made of a large number of birth and weaning weights collected as a normal routine at the Rowett Institute during the past few years.



Genetics ◽  
1991 ◽  
Vol 128 (3) ◽  
pp. 539-547 ◽  
Author(s):  
M Picard ◽  
R Debuchy ◽  
E Coppin

Abstract DNAs that encode the mating-type functions (mat+ and mat-) of the filamentous fungus Podospora anserina were cloned with the use of the mating-type A probe from Neurospora crassa. Cloning the full mat information was ascertained through gene replacement experiments. Molecular and functional analyses of haploid transformants carrying both mating types lead to several striking conclusions. Mat+ mat- strains are dual maters. However, the resident mat information is dominant to the mat information added by transformation with respect to fruiting body development and ascus production. Moreover, when dual mating mat+ mat- strains are crossed to mat+ or mat- testers, there is strong selection, after fertilization, that leads to the loss from the mat+ mat- nucleus of the mat information that matches that of the tester. Finally, the mat locus contains at least two domains, one sufficient for fertilization, the other necessary for sporulation.



Author(s):  
Steven J. DeRose

XML can be as easy to work with as JSON. However, this has not been obvious until now. JSON is easy because it supports only datatypes that are already native to Javascript and uses the same syntax to access them (such as [1:10], ["x"], and “.” notation). XML, on the other hand, supports additional datatypes, and is most commonly handled via SAX or DOM, both of which are low-level and meant to be cross-language. Typical developers want high-level access that feels “native” in the language they are using. These shortcomings have little or nothing to do with XML, and can be remedied by a different API. Software that demonstrates this is presented and described. It uses Python's richer set of abstract datatypes (such as tuples and sets), and provides native Python style syntax with richer semantics than JSON or Javascript.



Author(s):  
Davide Picca ◽  
Dominique Jaccard ◽  
Gérald Eberlé

In the last decades, Natural Language Processing (NLP) has obtained a high level of success. Interactions between NLP and Serious Games have started and some of them already include NLP techniques. The objectives of this paper are twofold: on the one hand, providing a simple framework to enable analysis of potential uses of NLP in Serious Games and, on the other hand, applying the NLP framework to existing Serious Games and giving an overview of the use of NLP in pedagogical Serious Games. In this paper we present 11 serious games exploiting NLP techniques. We present them systematically, according to the following structure:  first, we highlight possible uses of NLP techniques in Serious Games, second, we describe the type of NLP implemented in the each specific Serious Game and, third, we provide a link to possible purposes of use for the different actors interacting in the Serious Game.



2020 ◽  
Vol 3 ◽  
pp. 1
Author(s):  
Anumegha Gupta ◽  
Meenakshi Tiwari ◽  
Alka Sharma ◽  
Ashutosh N. Pandey ◽  
Pramod K. Yadav ◽  
...  

Oocyte meiosis is a prolong series of events that are comprised several intermittent channels in mammals. Oocyte meiosis starts during fetal life and then gets arrested at diplotene stage of first meiotic prophase in follicular oocyte. The continuous transfer of cyclic adenosine 3’, 5’-monophosphate (cAMP) and cyclic guanosine 3’, 5’-monophosphate (cGMP) from encircling granulosa cells to the oocyte through gap junctions helps in the maintenance of their high level required to achieve the long-lasting diplotene arrest so-called germinal vesicle stage. Phosphodiesterase inhibitors have been used to elevate intracellular level of both cyclic nucleotides and prevent spontaneous resumption of meiosis in oocytes under in vitro culture conditions. On the other hand, disruption of gap junction either by pituitary gonadotropin or by physical removal of encircling granulosa cells interrupts transfer of these nucleotides to the oocyte. As a result, intraoocyte cAMP as well as cGMP levels are decreased drastically that initiate downstream pathways to destabilize maturation-promoting factor (MPF). The destabilized MPF initiates meiotic resumption from diplotene arrest in mammalian oocytes. Oocyte meiosis further progresses from metaphase I to metaphase II stage and extrudes first polar body to get converted into haploid female gamete at the time of ovulation. Indeed, high level of cAMP as well as cGMP levels maintains diplotene arrest for a long time in follicular oocytes. On the other hand, transient decrease of their levels drives resumption from diplotene arrest, thereby meiotic maturation process, which enables oocyte to achieve developmental competency. Any defect in this process directly affects oocyte quality and thereby reproductive outcome in mammals including human.



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