scholarly journals Differential effects of auxin polar transport inhibitors on rooting in some Crassulaceae species

2014 ◽  
Vol 67 (2) ◽  
pp. 85-92 ◽  
Author(s):  
Marian Saniewski ◽  
Justyna Góraj ◽  
Elżbieta Węgrzynowicz-Lesiak ◽  
Kensuke Miyamoto ◽  
Junichi Ueda
Keyword(s):  
Mode Of Action ◽  
Root Formation ◽  
Endogenous Auxin ◽  
Differential Mode ◽  
Polar Transport ◽  
Differential Effects ◽  
Kalanchoe Blossfeldiana ◽  
Naphthylphthalamic Acid ◽  
Transport Inhibitors ◽  
Auxin Polar Transport

Effects of auxin polar transport inhibitors, 2,3,5-triio-dobenzoic acid (TIBA), 1-<em>N</em>-naphthylphthalamic acid (NPA) and methyl 2-chloro-9-hydroxyfluorene-9-carboxylate (morphactin IT 3456), as a lanolin paste, on root formation in cuttings of some species of Crassulaceae, such as <em>Bryophyllum daigremontianum, B. calycinum, Kalanchoe blossfeldiana </em>and <em>K. tubiflora</em>, were studied. Cuttings of these plants were easily rooted in water without any treatment. TIBA and morphactin IT 3456 completely inhibited root formation in the cuttings of these plants but NPA did not when these inhibitors were applied around the stem below the leaves. When TIBA and morphactin were applied around the stem near the top, but leaves were present below the treatment, the root formation was observed in <em>B. calycinum </em>and <em>K. blossfeldiana </em>but in a smaller degree than in control cuttings. These results strongly suggest that endogenous auxin is required for root formation in cuttings of Crassulaceae plants. The differential mode of action of NPA is discussed together with its effect on auxin polar transport.

Identification of dehydrocostus lactone and 4-hydroxy-β-thujone as auxin polar transport inhibitors

2013 ◽  
Vol 35 (7) ◽  
pp. 2251-2258 ◽  
Author(s):  
Junichi Ueda ◽  
Yuta Toda ◽  
Kiyotaka Kato ◽  
Yuichi Kuroda ◽  
Tsukasa Arai ◽  
...  
Keyword(s):  
Polar Transport ◽  
Transport Inhibitors ◽  
Auxin Polar Transport ◽  
Dehydrocostus Lactone

scholarly journals Morphactin stimulates stem elongation and thickening in decapitated shoots of Bryophyllum calycinum Salisb.

2013 ◽  
Vol 66 (2) ◽  
pp. 21-28 ◽  
Author(s):  
Kensuke Miyamoto ◽  
Agnieszka Marasek-Ciołakowska ◽  
Justyna Góraj ◽  
Elżbieta Węgrzynowicz-Lesiak ◽  
Junichi Ueda ◽  
...  
Keyword(s):  
Synergistic Effect ◽  
Growth Regulator ◽  
Stem Elongation ◽  
Specific Inhibitor ◽  
Endogenous Auxin ◽  
Simultaneous Application ◽  
Polar Transport ◽  
Physiological Processes ◽  
Auxin Polar Transport ◽  
Synthetic Growth

Morphactin, methyl 2-chloro-9-hydroxyfluorene-9-carboxylate (IT 3456), is a synthetic growth regulator with a unique action affecting various morphogenetic and physiological processes in plants, and has been characterized as a specific inhibitor of auxin polar transport. Morphactin applied at the last internode in decapitated shoots of <em>Bryophyllum calycinum</em> substantially stimulated elongation and thickening of the internode. Benzyladenine applied alone little affected stem elongation and thickening. Simultaneous application of benzyladenine and morphactin showed a synergistic effect on thickening, while it did not on elongation. These results suggest that morphactin translocated basipetally from the top of the treated internode inhibits auxin polar transport from the internode, resulting in the accumulation of endogenous auxin for elongation and thickening in the treated internode of decapitated shoots of <em>Bryophyllum calycinum</em>.

scholarly journals Effects of auxin polar transport inhibitors on the growth of the excised fourth internode in tulips

2013 ◽  
Vol 21 (2) ◽  
pp. 31-39
Author(s):  
Elżbieta Węgrzynowicz-Lesiak ◽  
Justyna Góraj ◽  
Kensuke Miyamoto ◽  
Junichi Ueda ◽  
Marian Saniewski
Keyword(s):  
Elongation Growth ◽  
Normal Position ◽  
Flower Bud ◽  
Promoting Effect ◽  
Inverted Position ◽  
Tulipa Gesneriana ◽  
Simultaneous Application ◽  
Polar Transport ◽  
Transport Inhibitors ◽  
Auxin Polar Transport

ABSTRACT Effects of auxin polar transport inhibitors: methyl 2-chloro-9-hydroxyfluorene-9-carboxylate (morphactin IT 3456), 2,3,5-triiodobenzoic acid (TIBA) and N-1-naphthylphthalamic acid (NPA) on elongation growth of the excised fourth internode of tulips (Tulipa gesneriana L.) cv. Apeldoorn were studied. After removal of flower bud, the continuing elongation of the excised fourth internode kept in water in normal or inverted position was observed, the elongation in inverted position being significantly higher than that in the normal position. On the other hand, the application of these inhibitors (0.2%, w/w in lanolin) in the place of removed flower bud substantially enhanced the elongation, the stimulation being much higher in the normal position than in the inverted one. When the inhibitors were applied in the middle or 1 cm from the base of the internode, the growth-promoting effect of these inhibitors was observed both in the upper and lower part of the internode, being greater in the upper part of the internode, regardless of the position of explants. Simultaneous application of indole-3-acetic acid (IAA) (0.1%, w/w in lanolin) at the place of the removed flower bud in the normal position with morphactin, TIBA and NPA applied in the middle or 1 cm from the base of the excised internode greatly stimulated the elongation, whereas almost no growthpromoting effect of these inhibitors was observed, in comparison to IAA. On the contrary, when IAA was applied on the base of the excised internode in the inverted position, the growth was inhibited, compared to that with lanolin only. The inhibitory effect of auxin was eliminated by the simultaneous application of morphactin, TIBA and NPA placed in the middle of the excised internode. These results suggest that auxin levels in the excised internode regulated by auxin polar transport play a crucial role in the regulation of its elongation growth.

scholarly journals Morphactin Substantially Induced the Fourth Internode Growth in Decapitated Tulips: Relevance to Endogenous Levels of Indole-3-Acetic Acid

2012 ◽  
Vol 20 (2) ◽  
pp. 161-171 ◽  
Author(s):  
Junichi Ueda ◽  
Justyna Góraj ◽  
Elżbieta Węgrzynowicz-Lesiak ◽  
Kensuke Miyamoto
Keyword(s):  
Mass Spectrometry ◽  
Acetic Acid ◽  
Liquid Chromatography ◽  
Stem Growth ◽  
Gas Liquid Chromatography ◽  
Liquid Chromatography Mass Spectrometry ◽  
Polar Transport ◽  
Internode Growth ◽  
Transport Inhibitors ◽  
Auxin Polar Transport

Abstract Important role of auxin polar transport inhibitors such as TIBA (2,3,5- triiodobenzoic acid), NPA (N-(1-naphthyl)phthalamic acid) and morphactin (methyl 2-chloro-9-hydroxyfluorene-9-carboxylate) in stem growth of tulips was intensively studied. After the application of morphactin above the 3rd node of decapitated tulips, the stimulated growth of the 4th internode was clearly observed. On the other hand, NPA and TIBA were slightly effective in stimulating the 4th internode growth of tulips. Endogenous levels of IAA in the 4th internode after the treatment of auxin polar transport inhibitors were determined using gas-liquid chromatography-mass spectrometry (GC-MS) and gas-liquid chromatography-mass spectrometry selected ion monitoring system (GC-SIM) with deuterium labelled IAA (Indole-2,4,5,6,7-d5-3- acetic acid, d5-IAA) as an internal standard, resulted in significant accumulation of IAA in the 4th internode of tulips after the treatment of morphactin. In the treatment of NPA and TIBA, there was almost no such an accumulation of IAA. These results strongly suggest that stimulated growth of the 4th internode of tulips induced by the application of morphactin is the consequence of accumulated endogenous levels of IAA after the treatment. The possible mode of action of auxin polar transport inhibitors in tulip stem growth is also discussed.

scholarly journals Auxin polar transport is essential for the development of zygote and embryo in Nicotiana tabacum L. and correlated with ABP1 and PM H+-ATPase activities

2010 ◽  
Vol 61 (6) ◽  
pp. 1853-1867 ◽  
Author(s):  
Dan Chen ◽  
Yujun Ren ◽  
Yingtian Deng ◽  
Jie Zhao
Keyword(s):  
Nicotiana Tabacum ◽  
Polar Transport ◽  
Nicotiana Tabacum L ◽  
Auxin Polar Transport

Requirement of the Auxin Polar Transport System in Early Stages of Arabidopsis Floral Bud Formation

1991 ◽  
Vol 3 (7) ◽  
pp. 677 ◽  
Author(s):  
Kiyotaka Okada ◽  
Junichi Ueda ◽  
Masako K. Komaki ◽  
Callum J. Bell ◽  
Yoshiro Shimura
Keyword(s):  
Transport System ◽  
Polar Transport ◽  
Bud Formation ◽  
Early Stages ◽  
Auxin Polar Transport ◽  
Floral Bud

scholarly journals Auxin minimum triggers the developmental switch from cell division to cell differentiation in the Arabidopsis root

2017 ◽  
Vol 114 (36) ◽  
pp. E7641-E7649 ◽  
Author(s):  
Riccardo Di Mambro ◽  
Micol De Ruvo ◽  
Elena Pacifici ◽  
Elena Salvi ◽  
Rosangela Sozzani ◽  
...  
Keyword(s):  
Cell Division ◽  
Auxin Transport ◽  
Positional Information ◽  
Polar Auxin Transport ◽  
Arabidopsis Root ◽  
Polar Transport ◽  
Stringent Control ◽  
Auxin Polar Transport ◽  
Multicellular Organisms ◽  
Developmental Switch

In multicellular organisms, a stringent control of the transition between cell division and differentiation is crucial for correct tissue and organ development. In the Arabidopsis root, the boundary between dividing and differentiating cells is positioned by the antagonistic interaction of the hormones auxin and cytokinin. Cytokinin affects polar auxin transport, but how this impacts the positional information required to establish this tissue boundary, is still unknown. By combining computational modeling with molecular genetics, we show that boundary formation is dependent on cytokinin’s control on auxin polar transport and degradation. The regulation of both processes shapes the auxin profile in a well-defined auxin minimum. This auxin minimum positions the boundary between dividing and differentiating cells, acting as a trigger for this developmental transition, thus controlling meristem size.

scholarly journals Differential effects of N-1-naphthylphthalamic acid (NPA) and 2,3,5-triiodobenzoic acid (TIBA) on auxin control of swelling of the shoots of Bryophyllum calycinum Salisb.

2017 ◽  
Vol 70 (3) ◽  
Author(s):  
Marian Saniewski ◽  
Justyna Góraj-Koniarska ◽  
Eleonora Gabryszewska ◽  
Kensuke Miyamoto ◽  
Junichi Ueda
Keyword(s):  
Acetic Acid ◽  
Differential Effect ◽  
Auxin Transport ◽  
Differential Mode ◽  
Treatment Area ◽  
Simultaneous Application ◽  
Triiodobenzoic Acid ◽  
Intact Plants ◽  
Naphthylphthalamic Acid ◽  
Indole 3 Acetic Acid

The effects of <em>N</em>-1-naphthylphthalamic acid (NPA) and 2,3,5-triiodobenzoic acid (TIBA) on the swelling of the stem in intact and decapitated plants of <em>Bryophyllum calycinum</em> in relation to the interaction with auxin, indole-3-acetic acid (IAA), are described. NPA induced conspicuous local internode swelling only in the area of its application in intact plants and in the decapitated internode in the case of simultaneous application of IAA on the top of the internode. By contrast, TIBA applied to an internode of intact plants induced swelling along the entire internode above the treatment area, and similar results were obtained in the decapitated internode when TIBA was applied in the middle of the internode and IAA was applied onto the top of the internode. The differential effect of NPA and TIBA on stem swelling in <em>B. calycinum</em> is discussed in relation to their differential mode of action on auxin transport.

scholarly journals Suitable Experimental Design for Determination of Auxin Polar Transport in Space Using a Spacecraft.

2000 ◽  
Vol 14 (1) ◽  
pp. 9-13 ◽  
Author(s):  
Toru Shimazu ◽  
Kensuke Miyamoto ◽  
Takayuki Hoson ◽  
Seiichiro Kamisaka ◽  
Junichi Ueda
Keyword(s):  
Experimental Design ◽  
Polar Transport ◽  
Auxin Polar Transport
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