scholarly journals Adipose-derived stem cells for tissue engineering and therapy of non-healing wounds

2018 ◽  
Vol 72 ◽  
pp. 806-821 ◽  
Author(s):  
Adriana Schumacher ◽  
Mirosława Cichorek ◽  
Michał Pikuła
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Wound Healing ◽  
Clinical Trials ◽  
Adipose Tissue ◽  
Growth Factors ◽  
Chronic Wounds ◽  
Clinical Effects ◽  
Adipose Derived Stem Cells ◽  
Healing Wounds

Chronic wounds seem to be a big problem for the medicinal, social and commercial area, especially for elder patients or people with cancer, metabolic or autoimmune diseases. In this respect, in the modern regenerative medicine there are intensive studies on methodologies that stimulate healing of chronic wounds (diabetic foots, ulcers, burns). In tissue engineering new solutions in wound healing are based on cellular therapies which consisting of growth factors and various types of scaffolds. In this way, there are created skin substitutes which are composed of cellular auto/allografts (stem cells and differentiated cells) and most commonly biodegradable scaffolds; they aim is not only to fill the tissue but also to stimulate wound healing. In this article we demonstrate the current knowledge about biological properties of Adipose- -derived Stem Cells (ASCs), methods of their isolation and potential for use in therapies for non-healing wounds. Adipose tissue seems to be an attractive and abundant stem cells source with therapeutic applicability in diverse phase of the repair and regeneration of the chronically damaged tissues. Additionally, it is believed that secreted by ASCs growth factors, cytokines and exosomes are decisive in the clinical effects. In this review, we also present the current clinical trials using stem cells derived from adipose tissue. Increasingly, the use of cell therapy in wound healing treatment draws attention to the safety, reproducibility and quality of stem cells. Researches go on and therapy approaches are possible but the detailed knowledge of the ASCs biology must be thoroughly investigated before these cells would be widely used in the clinical trials.

Adipose tissue engineering with naturally derived scaffolds and adipose-derived stem cells

Biomaterials ◽  
2007 ◽  
Vol 28 (26) ◽  
pp. 3834-3842 ◽  
Author(s):  
Lauren Flynn ◽  
Glenn D. Prestwich ◽  
John L. Semple ◽  
Kimberly A. Woodhouse
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Adipose Tissue ◽  
Adipose Derived Stem Cells ◽  
Adipose Tissue Engineering

scholarly journals Adipose Tissue-Derived Stem Cells: The Biologic Basis and Future Directions for Tissue Engineering

Materials ◽  
2020 ◽  
Vol 13 (14) ◽  
pp. 3210
Author(s):  
Diana Câmara ◽  
Jamil Shibli ◽  
Eduardo Müller ◽  
Paulo De-Sá-Junior ◽  
Allan Porcacchia ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Adipose Tissue ◽  
Human Leukocyte ◽  
Basic Research ◽  
Telomerase Activity ◽  
Adipose Derived Stem Cells ◽  
Precise Control ◽  
Leukocyte Antigens ◽  
Active Research

Mesenchymal stem cells (MSCs) have been isolated from a variety of tissues using different methods. Active research have confirmed that the most accessible site to collect them is the adipose tissue; which has a significantly higher concentration of MSCs. Moreover; harvesting from adipose tissue is less invasive; there are no ethical limitations and a lower risk of severe complications. These adipose-derived stem cells (ASCs) are also able to increase at higher rates and showing telomerase activity, which acts by maintaining the DNA stability during cell divisions. Adipose-derived stem cells secret molecules that show important function in other cells vitality and mechanisms associated with the immune system, central nervous system, the heart and several muscles. They release cytokines involved in pro/anti-inflammatory, angiogenic and hematopoietic processes. Adipose-derived stem cells also have immunosuppressive properties and have been reported to be “immune privileged” since they show negative or low expression of human leukocyte antigens. Translational medicine and basic research projects can take advantage of bioprinting. This technology allows precise control for both scaffolds and cells. The properties of cell adhesion, migration, maturation, proliferation, mimicry of cell microenvironment, and differentiation should be promoted by the printed biomaterial used in tissue engineering. Self-renewal and potency are presented by MSCs, which implies in an open-source for 3D bioprinting and regenerative medicine. Considering these features and necessities, ASCs can be applied in the designing of tissue engineering products. Understanding the heterogeneity of ASCs and optimizing their properties can contribute to making the best therapeutic use of these cells and opening new paths to make tissue engineering even more useful.

scholarly journals Adipose-Derived Stem Cells Secretome and Its Potential Application in “Stem Cell-Free Therapy”

Biomolecules ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 878
Author(s):  
Anna Trzyna ◽  
Agnieszka Banaś-Ząbczyk
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Stem Cell ◽  
Growth Factors ◽  
Treatment Strategies ◽  
Adipose Derived Stem Cells ◽  
Cell Based Therapy ◽  
Current State ◽  
Non Coding Rnas ◽  
New Treatment

Adipose-derived stem cells (ASCs) secrete many cytokines, proteins, growth factors, and extracellular vesicles with beneficial outcomes that can be used in regenerative medicine. It has great potential, and the development of new treatment strategies using the ASCs secretome is of global interest. Besides cytokines, proteins, and growth factors, the therapeutic effect of secretome is hidden in non-coding RNAs such as miR-21, miR-24, and miR-26 carried via exosomes secreted by adequate cells. The whole secretome, including ASC-derived exosomes (ASC-exos) has been proven in many studies to have immunomodulatory, proangiogenic, neurotrophic, and epithelization activity and can potentially be used for neurodegenerative, cardiovascular, respiratory, inflammatory, and autoimmune diseases as well as wound healing treatment. Due to limitations in the use of stem cells in cell-based therapy, its secretome with emphasis on exosomes seems to be a reasonable and safer alternative with increased effectiveness and fewer side effects. Moreover, the great advantage of cell-free therapy is the possibility of biobanking the ASCs secretome. In this review, we focus on the current state of knowledge on the use of the ASCs secretome in stem cell-free therapy.

scholarly journals The Role of Macrophage Migration Inhibitory Factor in Adipose-Derived Stem Cells Under Hypoxia

2021 ◽  
Vol 12 ◽  
Author(s):  
Elena Hofmann ◽  
Josefin Soppert ◽  
Tim Ruhl ◽  
Epameinondas Gousopoulos ◽  
Simona Gerra ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Migration Inhibitory Factor ◽  
Macrophage Migration Inhibitory Factor ◽  
Inhibitory Factor ◽  
Erk Signaling ◽  
Macrophage Migration ◽  
Adipose Derived Stem Cells ◽  
Tissue Samples ◽  
Healing Wounds

Background: Adipose-derived stem cells (ASCs) are multipotent mesenchymal stem cells characterized by their strong regenerative potential and low oxygen consumption. Macrophage migration inhibitory factor (MIF) is a multifunctional chemokine-like cytokine that is involved in tissue hypoxia. MIF is not only a major immunomodulator but also is highly expressed in adipose tissue such as subcutaneous adipose tissue of chronic non-healing wounds. In the present study, we investigated the effect of hypoxia on MIF in ASCs isolated from healthy versus inflamed adipose tissue.Methods: Human ASCs were harvested from 17 patients (11 healthy adipose tissue samples, six specimens from chronic non-healing wounds). ASCs were treated in a hypoxia chamber at <1% oxygen. ASC viability, MIF secretion as well as expression levels of MIF, its receptor CD74, hypoxia-inducible transcription factor-1α (HIF-1α) and activation of the AKT and ERK signaling pathways were analyzed. The effect of recombinant MIF on the viability of ASCs was determined. Finally, the effect of MIF on the viability and production capacity of ASCs to produce the inflammatory cytokines tumor necrosis factor (TNF), interleukin (IL)-6, and IL-1β was determined upon treatment with recombinant MIF and/or a blocking MIF antibody.Results: Hypoxic treatment inhibited proliferation of ASCs derived from healthy or chronic non-healing wounds. ASCs from healthy adipose tissue samples were characterized by a low degree of MIF secretion during hypoxic challenge. In contrast, in ASCs from adipose tissue samples of chronic non-healing wounds, secretion and expression of MIF and CD74 expression were significantly elevated under hypoxia. This was accompanied by enhanced ERK signaling, while AKT signaling was not altered. Recombinant MIF did stimulate HIF-1α expression under hypoxia as well as AKT and ERK phosphorylation, while no effect on ASC viability was observed. Recombinant MIF significantly reduced the secretion of IL-1β under hypoxia and normoxia, and neutralizing MIF-antibodies diminished TNF-α and IL-1β release in hypoxic ASCs.Conclusions: Collectively, MIF did not affect the viability of ASCs from neither healthy donor site nor chronic wounds. Our results, however, suggest that MIF has an impact on the wound environment by modulating inflammatory factors such as IL-1β.

Therapeutic Applications for Adipose-Derived Stem Cells in Wound Healing and Tissue Engineering

2018 ◽  
Vol 4 (2) ◽  
pp. 127-137 ◽  
Author(s):  
Michelle E. McCarthy ◽  
Theodore A. Brown ◽  
Joanna Bukowska ◽  
Bruce A. Bunnell ◽  
Trivia Frazier ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Wound Healing ◽  
Adipose Derived Stem Cells ◽  
Therapeutic Applications

Composite hydrogel scaffolds incorporating decellularized adipose tissue for soft tissue engineering with adipose-derived stem cells

Biomaterials ◽  
2014 ◽  
Vol 35 (6) ◽  
pp. 1914-1923 ◽  
Author(s):  
Hoi Ki Cheung ◽  
Tim Tian Y. Han ◽  
Dale M. Marecak ◽  
John F. Watkins ◽  
Brian G. Amsden ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Adipose Tissue ◽  
Soft Tissue ◽  
Composite Hydrogel ◽  
Adipose Derived Stem Cells ◽  
Hydrogel Scaffolds ◽  
Soft Tissue Engineering
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