Effects of Enzyme Treated Palm Kernel Expeller on Metabolizable Energy, Growth Performance, Villus Height and Digesta Viscosity in Broiler Chickens

Abstract Two experiments were conducted to investigate the effects of a combined α-galactosidase and xylanase preparation on nutrients digestibility and growth performance in broiler chickens. Experiment 1 had 240 broilers allocated to 3 treatments with the dietary supplementation of 0, 300 and 500 g/t of the enzyme combination. Diet and amino acid (AA) digestibility were assessed. Experiment 2 was a 2 × 3 (enzyme × diet) factorial arrangement with 10 replicates of 12 male broilers per replicate. Diets were based on corn-Soybean meal (SBM) diet and had 3 nutritional levels (normal, 2% apparent metabolizable energy (AME) and crude protein (CP) reduction, and 4% AME and CP reduction). Each of these diets was fed with or without enzyme supplementation. Growth performance, chyme viscosity, nutrients digestibility, and endogenous enzymes activity were assessed. In experiment 1, enzyme supplementation improved the digestibility of Ca (P = 0.025) and ileal digestibility of total AA, Pro, Alu, Ile, Lys, His, Thr, Glu, Val, Leu, Tyr and Phe (P < 0.05), and also tended to increase the AME of diets (P < 0.10). In experiment 2, broilers fed the corn-SBM diet with 4% nutrient reduction had better growth performance (P < 0.05), jejunal digesta viscosity at 42 days (P < 0.01), and lower digestibility of gross energy (GE) (P < 0.05) when compared to those fed the normal nutrient diet. Enzyme inclusion increased digestibility of CP (P = 0.044), GE (P = 0.009), raffinose (P < 0.001) and stachyose (P < 0.001), improved average daily gain (P = 0.031), and reduced jejunal digesta viscosity at 42 days (P = 0.011). Besides, similar improvements trend in amylase, trypsin, sucrase, and maltase activity with enzyme inclusion were observed as with energy. These data support that the enzyme supplementation increased nutrients and ileal amino acid digestibility, improved performance and endogenous enzymes activity.

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Effect of Palm Kernel Protein on Growth Performance and Meat Quality of Broiler Chickens

Tavukçuluk Araştırma Dergisi ◽

10.34233/jpr.707532 ◽

2020 ◽

Vol 17 (1) ◽

pp. 41-47

Author(s):

Nur Shahirah SHAHİDAN ◽

Teck LOH ◽

Mohamed ALSHELMANİ ◽

Chong HAU ◽

Fu LEE ◽

...

Keyword(s):

Growth Performance ◽

Meat Quality ◽

Broiler Chickens ◽

Palm Kernel ◽

Kernel Protein

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Insights Into the Proteomic Profile of Newly Harvested Corn and Metagenomic Analysis of the Broiler Intestinal Microbiota

10.21203/rs.3.rs-687305/v1 ◽

2021 ◽

Author(s):

Dafei Yin ◽

Youli Wang ◽

Liqun Wang ◽

Yuqin Wu ◽

Xiaoyi Bian ◽

...

Keyword(s):

Intestinal Microbiota ◽

Broiler Chickens ◽

Soluble Sugars ◽

Pasting Properties ◽

Metabolizable Energy ◽

Intestinal Morphology ◽

Energy Utilization ◽

Proteomic Profile ◽

Gut Health ◽

Digesta Viscosity

Abstract BackgroundThe use of newly harvested corn in feed causes wet droppings in broilers and increased feed cost which was termed as “new season grain problem”. The present study was conducted to evaluate the proteomic profile of newly harvested corn and the subsequent influence on intestinal microbiol community for broiler chickens. MethodsNewly harvested corn stored for either half a month (HM) or two months (TM) was used, and the pasting properties, total soluble sugars, and proteomic analysis technology was used to explore the influence of storage on natural aging corn properties. Additionally, seventy-two 7d Ross 308 male broiler chicken were fed with different stored corn. Apparent metabolizable energy (AME), digesta viscosity, intestinal morphology and microbiota were examined to explore the influence of feed corn storage on broiler chickens. ResultsPasting properties in the TM corn exhibited decreased viscoelastic properties. Proteomic studies found a total of 26 proteins that were differentially expressed between the two treatment groups. Proteins involved in starch and polysaccharides biosynthesis were upregulated in TM compared with HM. Chickens fed on TM diet had higher relative energy utilization compared to the HM birds. With increased corn storage, the relative digesta viscosity decreased significantly (P≤0.05). The total number of goblet cells and lymphocytes was lower in chickens fed the TM diet. The microbiota data showed that the TM chickens had decreased abundance of diarrheal bacteria such as Hungatella hathewayi and Bacteroides fragilis, and increased butyrate-producing bacteria such as Alistipes compared to the HM chickens. ConclusionsStorage of newly harvested corn induced the synthetic reaction of large molecules and changed the solubility of starch and protein with increasing soluble sugars and decreasing pasting properties that may improve the fermentation of intestinal microbiota, improve the energy utilization and protect gut health without the risk of diarrhea.

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Dietary Enteromorpha Polysaccharide Enhances Intestinal Immune Response, Integrity, and Caecal Microbial Activity of Broiler Chickens

Frontiers in Nutrition ◽

10.3389/fnut.2021.783819 ◽

2021 ◽

Vol 8 ◽

Author(s):

Teketay Wassie ◽

Zhuang Lu ◽

Xinyi Duan ◽

Chunyan Xie ◽

Kefyalew Gebeyew ◽

...

Keyword(s):

Immune Response ◽

Gut Microbiota ◽

Growth Performance ◽

Broiler Chickens ◽

Marine Algae ◽

Basal Diet ◽

Control Group ◽

Villus Height ◽

Crypt Depth ◽

Caecal Microbiota

Marine algae polysaccharides have been shown to regulate various biological activities, such as immune modulation, antioxidant, antidiabetic, and hypolipidemic. However, litter is known about the interaction of these polysaccharides with the gut microbiota. This study aimed to evaluate the effects of marine algae Enteromorpha (Ulva) prolifera polysaccharide (EP) supplementation on growth performance, immune response, and caecal microbiota of broiler chickens. A total of 200 1-day-old Ross-308 broiler chickens were randomly divided into two treatment groups with ten replications of ten chickens in each replication. The dietary treatments consisted of the control group (fed basal diet), and EP group (received diet supplemented with 400 mg EP/kg diet). Results showed that chickens fed EP exhibited significantly higher (P < 0.05) body weight and average daily gain than the chicken-fed basal diet. In addition, significantly longer villus height, shorter crypt depth, and higher villus height to crypt depth ratio were observed in the jejunal and ileal tissues of chickens fed EP. EP supplementation upregulated the mRNA expression of NF-κB, TLR4, MyD88, IL-2, IFN-α, and IL-1β in the ileal and jejunal tissues (P < 0.05). Besides, we observed significantly higher (P < 0.05) short-chain volatile fatty acids (SCFAs) levels in the caecal contents of the EP group than in the control group. Furthermore, 16S-rRNA analysis revealed that EP supplementation altered gut microbiota and caused an abundance shift at the phylum and genus level in broiler chicken. Interestingly, we observed an association between microbiota and SCFAs production. Overall, this study demonstrated that supplementation of diet with EP promotes growth performance, improves intestinal immune response and integrity, and modulates the caecal microbiota of broiler chickens. This study highlighted the application of marine algae polysaccharides as an antibiotic alternative for chickens. Furthermore, it provides insight to develop marine algae polysaccharide-based functional food and therapeutic agent.

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Effect of Antimicrobial Peptide Microcin J25 on Growth Performance, Immune Regulation, and Intestinal Microbiota in Broiler Chickens Challenged with Escherichia coli and Salmonella

Animals ◽

10.3390/ani10020345 ◽

2020 ◽

Vol 10 (2) ◽

pp. 345 ◽

Cited By ~ 3

Author(s):

Gang Wang ◽

Qinglong Song ◽

Shuo Huang ◽

Yuming Wang ◽

Shuang Cai ◽

...

Keyword(s):

Antimicrobial Peptide ◽

Growth Performance ◽

Intestinal Microbiota ◽

Immune Regulation ◽

Broiler Chickens ◽

Body Weight Gain ◽

The Body ◽

Villus Height ◽

Microcin J25 ◽

Challenge Group

The purpose of this study was to investigate the effects of antimicrobial peptide microcin J25 (MccJ25) on growth performance, immune regulation, and intestinal microbiota in broilers. A total of 3120 one-day-old male Arbor Acres (AA) broilers were randomly allocated to five groups (12 replicates, 52 chickens per replicate). The treatments were control, challenge (0 mg/kg MccJ25), different dosages of antimicrobial peptide (AMP) (0.5 and 1mg/kg MccJ25), and antibiotic groups (20 mg/kg colistin sulfate). The MccJ25 groups increased the body weight gain (starter and overall) that was reduced in the challenge group. The overall (day 1 to day 42) feed-to-gain ratio (G:F) was significantly decreased in AMP groups compared with the challenge group. Birds fed AMP had a decreased population of total anaerobic bacteria (day 21 and day 42) and E. coli (day 21 and day 42) in feces, as well as a lower Salmonella infection rate (day 21 and day 42) compared with birds in the challenge group. The villus height of the duodenum, jejunum, and ileum, as well as the villus height/crypt depth of the duodenum and jejunum were greater in AMP groups than birds in the challenge group. Moreover, MccJ25 linearly improved the villus height of the duodenum and jejunum. The addition of MccJ25 decreased the concentration of TNF-α, IL-1β, and IL-6 compared with challenge group. At d 21, MccJ25 linearly reduced the level of IL-6. In conclusion, dietary supplemented MccJ25 eﬀectively improved performance, systematic inﬂammation, and improved fecal microbiota composition of the broilers.

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Effect of oligosaccharides extract from palm kernel expeller on growth performance, gut microbiota and immune response in broiler chickens

Poultry Science ◽

10.3382/ps/pev216 ◽

2015 ◽

Vol 94 (10) ◽

pp. 2414-2420 ◽

Cited By ~ 27

Author(s):

Siamak Rezaei ◽

Mohammad Faseleh Jahromi ◽

Juan Boo Liang ◽

Idrus Zulkifli ◽

Abdoreza Soleimani Farjam ◽

...

Keyword(s):

Immune Response ◽

Gut Microbiota ◽

Growth Performance ◽

Broiler Chickens ◽

Palm Kernel

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Effects of flavomycin and probiotic supplementation to diets containing different sources of fat on growth performance, intestinal morphology, apparent metabolizable energy, and fat digestibility in broiler chickens

Poultry Science ◽

10.3382/ps.2011-01844 ◽

2012 ◽

Vol 91 (4) ◽

pp. 918-927 ◽

Cited By ~ 20

Author(s):

S.D. Sharifi ◽

A. Dibamehr ◽

H. Lotfollahian ◽

B. Baurhoo

Keyword(s):

Growth Performance ◽

Broiler Chickens ◽

Metabolizable Energy ◽

Intestinal Morphology ◽

Probiotic Supplementation ◽

Apparent Metabolizable Energy ◽

Different Sources ◽

Fat Digestibility

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Partition of metabolizable energy, and prediction of growth performance and lipid deposition in broiler chickens

Poultry Science ◽

10.3382/ps/pev081 ◽

2015 ◽

Vol 94 (6) ◽

pp. 1287-1297 ◽

Cited By ~ 6

Author(s):

B. Carré ◽

H. Juin

Keyword(s):

Growth Performance ◽

Broiler Chickens ◽

Metabolizable Energy ◽

Lipid Deposition

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Impact of exogenous carbohydrases and phytase on growth performance and nutrient digestibility in broilers

Canadian Journal of Animal Science ◽

10.4141/cjas2012-138 ◽

2013 ◽

Vol 93 (2) ◽

pp. 243-249 ◽

Cited By ~ 8

Author(s):

H. Lu ◽

S.A. Adedokun ◽

A. Preynat ◽

V. Legrand-Defretin ◽

P. A. Geraert ◽

...

Keyword(s):

Body Weight ◽

Growth Performance ◽

Broiler Chickens ◽

Body Weight Gain ◽

Negative Control ◽

Nutrient Digestibility ◽

Metabolizable Energy ◽

Feed Ratio ◽

Exogenous Enzyme ◽

Enzyme Supplementation

Lu, H., Adedokun, S. A., Preynat, A., Legrand-Defretin, V., Geraert, P. A., Adeola, O. and Ajuwon, K. M. 2013. Impact of exogenous carbohydrases and phytase on growth performance and nutrient digestibility in broilers. Can. J. Anim. Sci. 93: 243–249. The effect of supplementing corn–soybean-based diets with Rovabio®Max AP, an exogenous enzyme mix with carbohydrase (xylanase and β-glucanase) and phytase activities to broiler chickens was evaluated. Male Ross 708 broilers were assigned to four diets: positive control (PC), negative control (NC), deficient in metabolizable energy (ME), crude protein (CP), calcium (Ca) and non-phytate phosphorus (nPP), relative to the PC, by 100 kcal kg−1, 5 g kg−1, 1.1 g kg−1and 1.6 g kg−1, respectively, in the starter and grower diets; and NC+Rovabio®at 0.05 or 0.075 g kg−1diet. Starter diets were fed from day 0 to day 21 and grower diets from day 22 to day 42. Body weight (BW) and body weight gain on days 22 and 42 were increased (P<0.05) by enzyme supplementation. Enzyme addition increased (P<0.05) feed intake from day 22 to day 42 and overall (day 0 to day 42). Gain to feed ratio (G:F) was significantly increased (P<0.05) with enzyme supplementation during both starter and grower phases. Enzyme addition increased (P<0.05) serum phosphorus (P) concentration on day 21. On day 42 enzyme supplementation increased (P<0.05) the apparent ileal digestibility of P, dry matter (DM), energy, and N. Addition of a combination of carbohydrases (xylanase and β-glucanase) and phytase to corn–soybean-based diets deficient in ME, CP, Ca and P resulted in a significant increase in growth performance and utilization of P, DM, energy and N in broiler chickens.

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