Antioxidant Activity and Validation of Quantification Method for Lycopene Extracted from Tomato

2015 ◽  
Vol 98 (5) ◽  
pp. 1340-1345 ◽  
Author(s):  
Letícia Caramori Cefali ◽  
Edith Cristina Laignier Cazedey ◽  
Tatiana Maria Souza-Moreira ◽  
Marcos Antônio Correa ◽  
Hérida Regina Nunes Salgado ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Correlation Coefficient ◽  
Topical Formulations ◽  
Quantification Method ◽  
Identification And Quantification

Abstract Lycopene is a carotenoid found in tomatoes with potent antioxidant activity. The aim of the study was to obtain an extract containing lycopene from four types of tomatoes, validate a quantification method for the extracts by HPLC, and assess its antioxidant activity. Results revealed that the tomatoes analyzed contained lycopene and antioxidant activity. Salad tomato presented the highest concentration of this carotenoid and antioxidant activity. The quantification method exhibited linearity with a correlation coefficient of 0.9992. Tests for the assessment of precision, accuracy, and robustness achieved coefficients with variation of less than 5%. The LOD and LOQ were 0.0012 and 0.0039 μg/mL, respectively. Salad tomato can be used as a source of lycopene for the development of topical formulations, and based on performed tests, the chosen method for the identification and quantification of lycopene was considered to be linear, precise, exact, selective, and robust.

Identification and quantification of biochemical composition and antioxidant activity of walnut pollens

2021 ◽  
Vol 53 (6) ◽  
Author(s):  
Volkan Okatan ◽  
Ibrahim Bulduk ◽  
Bariş Kaki ◽  
Muhammet Ali Gundesli ◽  
Serhat Usanmaz ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Biochemical Composition ◽  
Identification And Quantification

scholarly journals Associations between pigments, antioxidant activity and coloring of blackberry fruits (Rubus sp.) and jellies through storage

2019 ◽  
pp. 1625-1630
Author(s):  
Angela Vacaro de Souza ◽  
Fernando Ferrari Putti ◽  
Marcos Ribeiro da Silva Vieira ◽  
Rogério Lopes Vieites
Keyword(s):  
Antioxidant Activity ◽  
Multivariate Analysis ◽  
Correlation Coefficient ◽  
Clustering Analysis ◽  
Cophenetic Correlation ◽  
Negative Relation ◽  
Linkage Method ◽  
Cophenetic Correlation Coefficient ◽  
The Mean ◽  
Generalized Distance

The objective of this study was to investigate the relations between the amount of anthocyanins, carotenoids and the antioxidant activity measured by the FRAP and TEAC methods. Furthermore, pigments and the coloration of blackberry harvested fruits were measured at 3 different collection points then fruits stored in refrigerated environment and the jelly made from them, preserved in hermetically sealed glasses, without contact with light and temperature of 25ºC. In order to investigate the relations between the study variables (content of anthocyanins and carotenoids, antioxidant activity and coloration using digital colorimeter), Pearson’s correlation analysis was adopted, which indicates the existence of a positive or negative relation between two variables. The α = 5% (correlation coefficient) was used to verify the significance of the correlation. The Mahalanobis (D2) generalized distance for the clustering analysis by the mean linkage method between group of blackberry fruits and jellies was applied. Furthermore, the cophenetic correlation coefficient and multivariate analysis using major components were applied to verify the grouping of different responses of harvesting points of fresh blackberry fruit in natura and jellies. The results showed that there was correlation between the content of anthocyanins and carotenoids in fruits (0.99*) and between the same parameters in jellies. However, this behavior was not clearly observed between the pigments and the antioxidant activity. There was a positive correlation between the factors involved in the coloring of chroma fruits ‘L’, ‘a’, ‘b’ and ºHue in fruits and jellies. Blackberry jellies presented as good sources of anthocyanins and carotenoids.

scholarly journals Dibutyl Phthalate an Emerging Contaminant in Water-Based Paints

2020 ◽  
Vol 63 (3) ◽  
pp. 139-146
Author(s):  
Ajoke Fehintola Idayat Apanpa-Qasim
Keyword(s):  
Solvent Extraction ◽  
Correlation Coefficient ◽  
Dibutyl Phthalate ◽  
Emerging Contaminant ◽  
Toxicological Effects ◽  
Water Based ◽  
Good Accordance ◽  
Southwest Nigeria ◽  
Cheap Alternative ◽  
Identification And Quantification

Seven different manufacturers of paints were purchased from two major cities in southwest Nigeria. At least one sample from the same manufacturer and colour, was identified and analyzed for dibutyl phthalate (DBP). GC/MS method was developed for the identification and quantification of dibutyl phthalate in water-based paints after solvent extraction. The highest level of DBP found was 47,100 ppb (unregistered manufacturer), followed by 45,100 and 19,400 in a white, pink and cream coloured paint, respectively, while the lowest concentration was 721 ppb in a cream coloured paints, unregistered manufacturer. The PCA results were in good accordance with the findings of the correlation coefficient. DBP is used by all manufacturers for flexibility and better spreadability. It is a cheap alternative and readily available. It's an emerging contaminant newly reported in paints and its toxicological effects should not be underestimated, since there is no regulation in place.

In vitro evaluation of quercetin cutaneous absorption from topical formulations and its functional stability by antioxidant activity

2007 ◽  
Vol 328 (2) ◽  
pp. 183-190 ◽  
Author(s):  
Rúbia Casagrande ◽  
Sandra R. Georgetti ◽  
Waldiceu A. Verri ◽  
Maria F. Borin ◽  
Renata F.V. Lopez ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Functional Stability ◽  
In Vitro Evaluation ◽  
Topical Formulations

scholarly journals IMCellXMBD: A statistical approach for robust cell identification and quantification from imaging mass cytometry images

2021 ◽  
Author(s):  
Xu Xiao ◽  
Naifei Su ◽  
Yan Kong ◽  
Lei Zhang ◽  
Xin Ding ◽  
...  
Keyword(s):  
Data Analysis ◽  
Biomedical Research ◽  
Background Noise ◽  
Statistical Approach ◽  
Mass Cytometry ◽  
Cell Identification ◽  
Quantification Method ◽  
Protein Channel ◽  
Protein Channels ◽  
Identification And Quantification

ImagingMass Cytometry (IMC) has become a useful tool in biomedical research due to its capability to measure over 100 markers simultaneously. Unfortunately, some protein channels in IMC images can be very noisy, whichmay significantly affect the phenotyping results without proper data processing. We developed IMCellXMBD, a highly effective and generalizable cell identification and quantification method for IMC images. IMCell performs denoising by subtracting an estimated background noise value from pixel values for each individual protein channel, identifies positive cells from negative cells by comparing the distribution between segmented cells and decoy cells, and normalize the protein expression levels of the identified positive cells for downstream data analysis. Experimental results demonstrate that our method significantly improves the reliability of cell phenotyping which is essential for using IMC in biomedical studies.

scholarly journals POLYPHENOLS AND ANTIOXIDANT ACTIVITY OF FOUR FRUITS NATIVE TO THE COAST OF CEARA UNDER DIFFERENT MATURATION STAGES

2017 ◽  
Vol 39 (1) ◽  
Author(s):  
NIGÉRIA PEREIRA GONÇALVES ◽  
ELISEU MARLÔNIO PEREIRA DE LUCENA ◽  
ORIEL HERRERA BONILLA ◽  
MÁRCIA RÉGIA SOUZA DA SILVEIRA
Keyword(s):  
Antioxidant Activity ◽  
Correlation Coefficient ◽  
Botanical Garden ◽  
Polyphenolic Compounds ◽  
Statistical Analyses ◽  
Total Antioxidant Activity ◽  
Plant Ecophysiology ◽  
Polyphenolic Content ◽  
Total Antioxidant ◽  
Maturation Stages

ABSTRACT This study aimed to quantify polyphenolic compounds and antioxidant activity of four fruits native to the coast of Ceara under different maturation stages aiming their use for a healthier diet. Myrtle (Eugenia punicifolia (Kunth) DC.) fruits were collected at the Botanical State Park of Ceara, in Caucaia-CE and guajiru (Chrisobalanus icaco L.), manipuça (Mouriri cearensis Huber) and murici-pitanga (Byrsonima gardneriana A. Juss.) fruits were collected at the Botanical Garden of São Gonçalo, São Gonçalo do Amarante-CE. Fruits were collected and transported to the Laboratory of Plant Ecophysiology, being characterized, processed and frozen for chemical assessments at the Laboratory of Physiology and Postharvest Technology - Embrapa Agroindústria Tropical: polyphenolic compounds and total antioxidant activity. The ESTAT software was used for statistical analyses. The polyphenolic content had the highest and the lowest value for guajiru fruits with 480.73 and 10.90 mg/100 g, respectively and total antioxidant activity, the highest value was found for manipuça fruits, with 42.99 µM of Trolox/g of pulp and the lowest value for guajiru fruits, with 1.45 µM of Trolox/g of pulp. There was a significant correlation between polyphenolic content and total antioxidant activity obtained for guajiru fruits, which showed high correlation coefficient, R = -0.95 (P < 0.05).

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