Applications of Novel Direct Bioautography Tests for Analysis of Antimicrobials: A Review

Abstract TLC coupled to direct bioautography detection can be applied to the analysis of various antimicrobial agents in complex matrixes. Because of the lack of commercially available microbiological detection methods, two direct bioautography tests were developed in our laboratory to be used after TLC separation. One method was based on Gram-positive bacteria, Bacillus subtilis, and the other on Gram-negative bacteria, Escherichia coli. These tests can be used for detection and determination of wide spectrum of antimicrobials as well as for other, nontypical purposes, such as choosing the best sample preparation method before the analysis. Some of the more interesting applications of the newly developed tests are described in this paper.

Download Full-text

Development of a high throughput 96-well plate sample preparation method for the determination of trileptal (oxcarbazepine) and its metabolites in human plasma

Journal of Chromatography B Biomedical Sciences and Applications ◽

10.1016/s0378-4347(01)00289-4 ◽

2001 ◽

Vol 762 (1) ◽

pp. 9-15 ◽

Cited By ~ 20

Author(s):

C Souppart ◽

M Decherf ◽

H Humbert ◽

G Maurer

Keyword(s):

Sample Preparation ◽

Human Plasma ◽

High Throughput ◽

Preparation Method ◽

Sample Preparation Method

Download Full-text

Determination of trans fatty acids in foodstuffs by gas chromatography-mass spectrometry after simultaneous microwave-assisted extraction-esterification

Journal of the Serbian Chemical Society ◽

10.2298/jsc090717051k ◽

2010 ◽

Vol 75 (6) ◽

pp. 803-812 ◽

Cited By ~ 4

Author(s):

Snezana Kravic ◽

Zvonimir Suturovic ◽

Jaroslava Svarc-Gajic ◽

Zorica Stojanovic ◽

Mira Pucarevic

Keyword(s):

Mass Spectrometry ◽

Sample Preparation ◽

Preparation Method ◽

Reference Method ◽

Gas Chromatography Mass Spectrometry ◽

Microwave Assisted Extraction ◽

Sample Preparation Method ◽

Microwave Assisted ◽

Assisted Extraction

A sample preparation method based on the simultaneous microwave-assisted extraction-esterification (SMAEE) was developed for the determination of the fatty acid composition of foodstuffs by gas chromatography-mass spectrometry. The proposed sample preparation method was validated by comparison with the reference Soxhlet extraction method followed by derivatisation by ester formation and the same determination step. The fatty acid compositions and the extraction efficiencies obtained using the proposed SMAEE method and the reference method were statistically similar. The results showed that compared to the conventional method, the SMAEE method offered the advantages of short sample preparation time, low consumption of expensive organic solvents and lower energy consumption. This good agreement between results provided by both the SMAEE and the reference method demonstrates the usefulness of the former as a routine method for the treatment of food samples prior to trans fatty analysis.

Download Full-text

Green and efficient sample preparation method for the determination of catalyst residues in margarine by ICP-MS

Talanta ◽

10.1016/j.talanta.2017.06.037 ◽

2017 ◽

Vol 174 ◽

pp. 394-400 ◽

Cited By ~ 9

Author(s):

Carla Andrade Hartwig ◽

Rodrigo Mendes Pereira ◽

Diogo La Rosa Novo ◽

Dirce Taina Teixeira Oliveira ◽

Marcia Foster Mesko

Keyword(s):

Sample Preparation ◽

Preparation Method ◽

Sample Preparation Method ◽

Icp Ms

Download Full-text

A small-scale sample preparation method with HPLC analysis for determination of tocopherols and tocotrienols in cereals

Journal of Food Composition and Analysis ◽

10.1016/j.jfca.2003.09.014 ◽

2004 ◽

Vol 17 (6) ◽

pp. 749-765 ◽

Cited By ~ 95

Author(s):

M. Ryynänen ◽

A.-M. Lampi ◽

P. Salo-Väänänen ◽

V. Ollilainen ◽

V. Piironen

Keyword(s):

Sample Preparation ◽

Hplc Analysis ◽

Preparation Method ◽

Small Scale ◽

Sample Preparation Method

Download Full-text

A multiresidue method for the determination of 109 pesticides in rice using the Quick Easy Cheap Effective Rugged and Safe (QuEChERS) sample preparation method and gas chromatography/mass spectrometry with temperature control and vacuum concentration

Rapid Communications in Mass Spectrometry ◽

10.1002/rcm.3199 ◽

2007 ◽

Vol 21 (18) ◽

pp. 3115-3122 ◽

Cited By ~ 57

Author(s):

Thanh Dong Nguyen ◽

Byung Soo Lee ◽

Bo Reum Lee ◽

Dae Myung Lee ◽

Gae-Ho Lee

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Sample Preparation ◽

Temperature Control ◽

Preparation Method ◽

Gas Chromatography Mass Spectrometry ◽

Sample Preparation Method ◽

Multiresidue Method ◽

Chromatography Mass Spectrometry

Download Full-text

ANTIMICROBIAL ACTIVITY OF COPPER KAOLINITE AND SURFACTANT MODIFIED COPPER KAOLINITE AGAINST GRAM POSITIVE AND GRAM NEGATIVE BACTERIA

Jurnal Teknologi ◽

10.11113/jt.v78.7827 ◽

2016 ◽

Vol 78 (3-2) ◽

Author(s):

Nor Syafawani Sarah Md Saad ◽

Nik Ahmad Nizam Nik Malek ◽

Chun Shiong Chong

Keyword(s):

Antimicrobial Activity ◽

Synergistic Effects ◽

Wide Spectrum ◽

Copper Nitrate ◽

Gram Negative Bacteria ◽

Gram Positive ◽

Gram Negative ◽

X Ray ◽

Gram Positive Bacteria ◽

Diffusion Technique

The aim of this research was to determine the antimicrobial activity of kaolinite modified with antimicrobial compounds against Gram positive and Gram negative bacteria. Copper kaolinite (Cu-kaolinite) was prepared by loading raw kaolinite with copper nitrate trihydrate (CuNO3) while surfactant modified Cu-kaolinite (SM-Cu-kaolinite) was prepared by adding cationic surfactants hexadecyltrimethyl ammonium (HDTMA) on Cu-Kaolinite. Samples was characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM) and energy dispersive X-ray (EDX) analyzer. The antimicrobial activity of the samples was tested against Gram negative bacteria (Escherichia coli ATCC 11229 and Pseudomonas aeruginosa ATCC 15442), and Gram positive bacteria (Staphylococcus aureus ATCC 6538 and Enterococcus faecalis ATCC 29212) through disc diffusion technique (DDT) and minimum inhibition concentration (MIC). The results showed that the antimicrobial activity of Cu-kaolinite increased after modified with HDTMA due to the synergistic effects of Cu ions and HDTMA molecules on the kaolinite. The antimicrobial activity for surfactant modified Cu-kaolinite was greater for Gram positive bacteria compared to Gram negative bacteria. In conclusion, the attachment of HDTMA on Cu-kaolinite contributed to the enhanced antimicrobial activity against wide spectrum of bacteria (Gram positive and Gram negative bacteria).

Download Full-text