scholarly journals Comparison of Native and Regenerated Antheraea Pernyi Silk Fibroin

2021 ◽  
Vol 27 (1) ◽  
pp. 57-62
Author(s):  
Yu DUAN ◽  
Xin CHEN ◽  
Zhengzhong SHAO
Keyword(s):  
Silk Fibroin ◽  
Nitrate Solution ◽  
Calcium Nitrate ◽  
Silk Fibers ◽  
Antheraea Pernyi ◽  
Rheological Measurements ◽  
Silk Fibroin Film ◽  
Bombyx Mori Silk ◽  
Helix Conformation ◽  
Α Helix

The regenerated Antheraea pernyi silk fibroin (RASF) was harvested by dissolving the silk fibers in a calcium nitrate solution. XRD result demonstrated that both native and regenerated Antheraea pernyi silk fibroin involved the α-helix conformation, and DTG curves showed that their thermal decompositions were quite similar and proceeded three steps. However, rheological measurements figured out that the molecule weight of RASF decreased from 246 kDa to 199 kDa, comparing with native Antheraea pernyi silk fibroin. Also, the tensile properties of the RASF film, which were the same to those of regenerated Bombyx mori silk fibroin film, were observed.

scholarly journals Glycerin-Induced Conformational Changes in Bombyx mori Silk Fibroin Film Monitored by 13C CP/MAS NMR and 1H DQMAS NMR

2016 ◽  
Vol 17 (9) ◽  
pp. 1517 ◽  
Author(s):  
Tetsuo Asakura ◽  
Masanori Endo ◽  
Misaki Hirayama ◽  
Hiroki Arai ◽  
Akihiro Aoki ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Silk Fibroin ◽  
Conformational Changes ◽  
Mas Nmr ◽  
Silk Fibroin Film ◽  
Bombyx Mori Silk ◽  
Cp Mas Nmr ◽  
13C Cp Mas Nmr

Self-assembly of monolayered lipid membranes for surface-coating of a nanoconfined Bombyx mori silk fibroin film

RSC Advances ◽  
2015 ◽  
Vol 5 (81) ◽  
pp. 65684-65689 ◽  
Author(s):  
Fan Xu ◽  
Meimei Bao ◽  
Longfei Rui ◽  
Jiaojiao Liu ◽  
Jingliang Li ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Silk Fibroin ◽  
Self Assembly ◽  
Lipid Membranes ◽  
Surface Coating ◽  
Lipid Membrane ◽  
Coating Film ◽  
Silk Fibroin Film ◽  
Bombyx Mori Silk ◽  
Self Assembled

A self-assembled lipid membrane provides a smooth, hydrophilic and biocompatible surface coating film for materials.

Antheraea pernyi Silk Fibroin Nanoparticles for Drug Delivery

2014 ◽  
Vol 27 ◽  
pp. 75-81 ◽  
Author(s):  
Shen Zhou Lu ◽  
Juan Wang ◽  
Li Mao ◽  
Gui Jun Li ◽  
Jian Jin
Keyword(s):  
Drug Delivery ◽  
Silk Fibroin ◽  
Molecular Conformation ◽  
Ph Value ◽  
Drug Loading ◽  
Antheraea Pernyi ◽  
Silk Fibroin Nanoparticles ◽  
Loading Ratio ◽  
Α Helix ◽  
Average Size

Silk nanoparticles were easily obtained from regenerated Antheraea Pernyi Silk Fibroin (ASF). The morphology and average size of the silk particles was sensitive to pH value of fibroin solution. The diameter of nanoparticles prepared was in the range of 30 nm to 1000 nm with a narrow size distribution. On this process, the molecular conformation of regenerated ASF changed from α-helix to β-sheet structure. The shape of prepared nanoparticles were regular spherical structure when the pH value was about 4.3 (pI) in ASF solution. Doxorubicin hydrochloride (DOX) was loaded in the ASF particles as drug release model and the drug-loading ratio was 3.4 %. The release rate of DOX from ASF nanoparticles was pH sensitive. After 23 days release, there was still 84% DOX in the ASF nanoparticles. The result suggested that the ASF nanoparticles might be suitable microcarriers for drug delivery.

scholarly journals Bombyx mori Silk Fibroin Scaffolds with Antheraea pernyi Silk Fibroin Micro/Nano Fibers for Promoting EA. hy926 Cell Proliferation

Materials ◽  
2017 ◽  
Vol 10 (10) ◽  
pp. 1153 ◽  
Author(s):  
Yongchun Chen ◽  
Weichao Yang ◽  
Weiwei Wang ◽  
Min Zhang ◽  
Mingzhong Li
Keyword(s):  
Cell Proliferation ◽  
Bombyx Mori ◽  
Silk Fibroin ◽  
Antheraea Pernyi ◽  
Bombyx Mori Silk

scholarly journals Structure of Silk I (Bombyx mori Silk Fibroin before Spinning) -Type II β-Turn, Not α-Helix-

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3706
Author(s):  
Tetsuo Asakura
Keyword(s):  
Physical Properties ◽  
Bombyx Mori ◽  
Silk Fibroin ◽  
Structural Model ◽  
Type Ii ◽  
Bombyx Mori Silk ◽  
Turn Structure ◽  
Α Helix ◽  
Ir And Raman ◽  
Proteins And Peptides

Recently, considerable attention has been paid to Bombyx mori silk fibroin by a range of scientists from polymer chemists to biomaterial researchers because it has excellent physical properties, such as strength, toughness, and biocompatibility. These appealing physical properties originate from the silk fibroin structure, and therefore, structural determinations of silk fibroin before (silk I) and after (silk II) spinning are a key to make wider applications of silk. There are discrepancies about the silk I structural model, i.e., one is type II β-turn structure determined using many solid-state and solution NMR spectroscopies together with selectively stable isotope-labeled model peptides, but another is α-helix or partially α-helix structure speculated using IR and Raman methods. In this review, firstly, the process that led to type II β-turn structure by the authors was introduced in detail. Then the problems in speculating silk I structure by IR and Raman methods were pointed out together with the problem in the assignment of the amide I band in the spectra. It has been emphasized that the conformational analyses of proteins and peptides from IR and Raman studies are not straightforward and should be very careful when the proteins contain β-turn structure using many experimental data by Vass et al., (Chem. Rev., 2003, 103, 1917–1954). In conclusion, the author emphasized here that silk I structure should be type II β-turn, not α-helix.

Study on Antheraea Pernyi Silk Fibroin Porous Materials

2011 ◽  
Vol 332-334 ◽  
pp. 1718-1721
Author(s):  
Li Mao ◽  
Xi Long Wu ◽  
Yu Liu ◽  
Shen Zhou Lu
Keyword(s):  
Porous Materials ◽  
Silk Fibroin ◽  
Average Pore Size ◽  
Diffraction Method ◽  
Random Coil ◽  
X Ray Diffraction ◽  
Average Pore ◽  
Antheraea Pernyi ◽  
Thiocyanate Solution ◽  
Α Helix

Antheraea pernyi silk fibroin (ASF) solution was prepared by dissolving Antheraea pernyi silk fiber in lithium thiocyanate solution. The ASF/1,4-butanediol (ASF/BDO) blend porous materials were prepared with freeze-drying method. The structure of ASF was investigated by the X-ray diffraction method. The result showed that the structure of regenerated ASF scaffold was α-helix and random coil conformation. After ASF mixed with 5 wt% BDO, the structure of ASF was changed to β-sheet and the ASF scaffolds became water-insoluble. There were some small pores and fibrous structure in the big pores and the surface of pores was rough with a great many raised particles. The scaffolds with the average pore size of 300-1000μm and the porosity of 82-92% can efficiently be produced in this paper. Due to avoid the use of organic solvents or harsh chemicals, these new ASF based porous materials provide much more excellent biocompatibility and is expected to be applied to tissue regeneration scaffolds.

Structure of Antheraea pernyi/Bombyx mori Silk Fibroin Scaffolds

2012 ◽  
Vol 535-537 ◽  
pp. 2321-2325 ◽  
Author(s):  
Xiufang Wu ◽  
Yu Hong Jiao ◽  
Gui Yang Liu ◽  
Shu Qin Yan ◽  
Ming Zhong Li
Keyword(s):  
Pore Structure ◽  
Bombyx Mori ◽  
Silk Fibroin ◽  
Pore Diameter ◽  
Tissue Engineering Scaffolds ◽  
Average Pore ◽  
Antheraea Pernyi ◽  
Condensed Structure ◽  
Α Helix ◽  
Blend Ratios

As the tissue engineering scaffolds, the pore structure and condensed structure of silk fibroin scaffolds should be adjusted and controlled. In this study, Antheraea pernyi/Bombyx mori (A. p/B. m) silk fibroin blend scaffolds were prepared by freeze-drying. The influence of blend ratios on the pore structure and condensed structure of the scaffolds was investigated. The results showed that the average pore diameter of the blend scaffolds changed from 56 to 326 μm. Due to the difference of properties and the macromolecules aggregation status of two silk fibroin solutions, the pore diameter, content of α-helix and crystallinity of the scaffolds decreased with the increasing of the proportion of B. m silk fibroin. By adjusting the blend ratios, the pore structure and condensed structure of A. p/B. m silk fibroin blend scaffolds could be controlled.

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