Acute Toxicity Profiles of Aqueous and Ethanolic Extracts of Capsicum annum Seeds from South Western Uganda

The overall objective of our study is to evaluate the toxicity of ethanolic extracts of Hexalobus monopetalus leaves on the liver and kidney functions of rats in its therapeutic use. Thus, an ethanolic extract of the leaves was made, then a phytochemical screening of this extract. On the one hand, acute toxicity was measured in a 14-day feeding trial in Wistar rats, and on the other hand, a histopathological study of the organs removed was performed. The phytochemical screens of the ethanolic extracts obtained showed the presence of several phytochemical groups with therapeutic activity. Acute toxicity was noted at 3000 and 5000mg / kg PC doses of rats. Histopathological study revealed hepatic pycnosis at a dose of 5000 mg / kg PC in rats. Finally, no renal damage was observed

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Acute toxicity, anti-inflammatory, analgesic and antipyretic effects of aqueous and hydroethanolic extracts of Brenania brieyi (Rubiaceae)

Open Access Research Journal of Biology and Pharmacy ◽

10.53022/oarjbp.2021.3.1.0050 ◽

2021 ◽

Vol 3 (1) ◽

pp. 019-032

Author(s):

Nkundineza JC ◽

Nsonde Ntandou GF ◽

Boumba LS ◽

Kibamgou S ◽

Motondo E ◽

...

Keyword(s):

Acetic Acid ◽

Acute Toxicity ◽

Aqueous Extract ◽

Ethanolic Extract ◽

Tail Flick ◽

Pharmacological Effects ◽

Tail Flick Test ◽

Ethanolic Extracts ◽

Anti Inflammatory ◽

Inflammatory Effect

Brenania brieyi (Rubiaceae) is widely used in traditional Congolese medicine in the treatment of many pathologies that are manifested by inflammation, pain and fever. The objective of this study was to study the acute toxicity as well as to evaluate the antipyretic, analgesic and anti-inflammatory effects of the aqueous and hydro-ethanolic extracts of Brenania brieyibark on models of pyrexia, algesia and inflammation induced in rodents. The aqueous extract of Brenania brieyidoes not cause any mortality up to the dose of 4000 mg/kg, but promotes a slight increase in body weight. From 2000 mg/kg, the signs of toxicity observed were the significant decrease in mobility as well as the loss of alertness. At doses of 100 and 200 mg/kg, aqueous and hydro-ethanolic Brenania brieyiextracts showed a very significant anti-inflammatory effect (***p< 0.001) on edemas induced by carrageenin (1%), formaldehyde (2.5%) and histamine (1 mg/mL), greater than that of diclofenac at 10 mg/kg. At 200 mg/kg, both extracts showed a very significant analgesic effect (***p< 0.001), greater than that of paracetamol 100 mg/kg against pain induced by acetic acid 0.6% and formaldehyde 2.5%. Brenania brieyiwas slightly effective in the tail flick test. Brewer's yeast-induced hyperthermia was reduced by both extracts. However, the hydro-ethanolic extract proves to be more effective than the aqueous extract in all the tests carried out. These pharmacological effects would be related to the presence of alkaloids, tannins, flavonoids, anthraquinones, oses and saponosides.

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Evaluation of Toxicity of Ethanolic Extracts of Piper guineense and Capsicum annum on Dermestid Beetle, Demestes maculatus (DeGeer 1774), a Pest on Dried Catfish, Clarias gariepinus

Pakistan Journal of Zoology ◽

10.17582/journal.pjz/2018.50.4.1313.1317 ◽

2018 ◽

Vol 50 (4) ◽

Author(s):

Felicia Nkechi Ekeh ◽

Ifeanyi Oscar Ndimkaoha Aguzie ◽

Joy Ihuoma Nzei ◽

Chinenye Maria-Goretti Ohanu ◽

Godwin Ikechukwu Ngwu ◽

...

Keyword(s):

Clarias Gariepinus ◽

Piper Guineense ◽

Capsicum Annum ◽

Ethanolic Extracts

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Evaluation of antibacterial activity and acute toxicity of pomegranate (punica granatum l.) seed ethanolic extracts in swiss webster mice

Journal of Dentomaxillofacial Science ◽

10.15562/jdmfs.v2i2.536 ◽

2017 ◽

Vol 2 (2) ◽

pp. 119

Author(s):

Riani Setiadhi ◽

Irna Sufiawati ◽

Dewi Zakiawati ◽

Nanan Nur’aeny ◽

Wahyu Hidayat ◽

...

Keyword(s):

Body Weight ◽

Acute Toxicity ◽

Ethanolic Extract ◽

Lethal Effect ◽

Punica Granatum ◽

Control Group ◽

Toxic Dose ◽

Streptococcus Sanguis ◽

Ethanolic Extracts ◽

Pomegranate Seeds

Objective: To evaluate the safety of a pomegranate seeds ethanolic extract in Swiss Webster mice.Material and Methods: Pomegranate seed’s ethanolic extracts were evaluated for minimum inhibitory concentration against streptococcus sanguis ATCC 10556, using microdilution method through 96-wells microplate. In acute toxicity test, 12 females and 12 males Swiss Webster mice weighing 20–30 grams were divided into four groups, each consisting of 3 females and 3 males. Three groups were orally given of sample with concentrations 0.2% equivalent to 40 mg/kg, 0.4% equivalent to 80 mg/kg, and 0.8% equivalent to 160 mg/kg, while the control group was given distilled water. Observations were made at 30, 60, and 90 minutes, for 24 hours and 7 days.Results: The MIC against Streptococcus sanguis was on 2000 ppm concentration and until 24 hours observation no lethal effect occurred on the mice, but at the 7 days observation in the group of 0.8% per kilogram body weight, the lethal effect occurred at one mice.Conclusion: The ethanolic extract of pomegranate seeds has antibacterial effect and no acute systemic toxicity, which can be considered safe on the use as oral topical solution since the toxic dose is greater than 160 mg/kg of body weight.

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In Vivo Toxicity Study of Ethanolic Extracts of Evolvulus alsinoides & Centella asiatica in Swiss Albino Mice

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2019.209 ◽

2019 ◽

Vol 7 (7) ◽

pp. 1071-1076

Author(s):

Mukesh Kumar Yadav ◽

Santosh Kumar Singh ◽

Manish Singh ◽

Shashank Shekhar Mishra ◽

Anurag Kumar Singh ◽

...

Keyword(s):

Single Dose ◽

Acute Toxicity ◽

Centella Asiatica ◽

Toxicity Study ◽

Histopathological Analysis ◽

Acute Administration ◽

In Vivo Toxicity ◽

Evolvulus Alsinoides ◽

Ethanolic Extracts

AIM: We aimed to investigate several parameters after the in vivo acute and sub-acute administration of ethanolic extracts from E. alsinoides & C. asiatica. METHODS: Malignant Ovarian Germ Cell Tumors for in vivo toxicity study guidelines 423 and 407 of Organization for Economic Co-operation and Development (OECD) were followed for acute and sub-acute toxicity assays respectively. For LD50 evaluation, a single dose of ethanolic extracts of Evolvulus alsinoides L. (EEA) and ethanolic extracts of Centella asiatica (ECA) was orally administered to mice at doses of 200, 400, 800, 1600 and 2000 mg/kg. Then the animals were observed for 72 hours. For acute toxicity evaluation, a single dose of both extracts was orally administered to mice at doses of 300, 600, 1200 and 2000 mg/kg and the animals were observed for 14 days. In the sub-acute study, the extracts were orally administered to mice for 28 days at doses of 300, 600, 1200 and 2000 mg/kg. To assess the toxicological effects, animals were closely observed on general behaviour, clinical signs of toxicity, body weight, food and water intake. At the end of the study, it was performed biochemical and hematological evaluations, as well as histopathological analysis from the following organs: brain, heart, liver, and kidney. RESULTS: The oral administration of E. alsinoides and C. asiatica ethanolic extracts, i.e. EEA 300, EEA 600, EEA 1200, EEA 2000, ECA 300, ECA 600, ECA 1200 & ECA 2000 mg/kg doses showed no moral toxicity effect in LD50, acute and sub-acute toxicity parameters. CONCLUSION: In this study, we had found that E. alsinoides & C. asiatica extract at different doses cause no mortality in acute and sub-acute toxicity study. Also, histopathology of kidney, liver, heart, and brain showed no alterations in tissues morphology.

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