scholarly journals Fungal Contaminants and Mycotoxins in Nuts

2021 ◽  
Author(s):  
Giulia Mirabile ◽  
Patrizia Bella ◽  
Antonio Vella ◽  
Vincenzo Ferrantelli ◽  
Livio Torta

Contamination by fungi and mycotoxins in nuts has achieved much attention in recent years. In fact, the fungal metabolites produced by the species of Aspergillus, Penicillium (aflatoxins and ochratoxins), Fusarium (trichothecenes, zearalenones and fumonisins) and Alternaria (alternariotoxins) with toxic and/or carcinogenic effects are considered a threat to human and animal health. In this chapter we will discuss the main fungal taxa and related mycotoxins most frequently associated with these materials. In this regard, the first results on the level of contamination by fungi and mycotoxins in samples of almonds and pistachios of different origins will be reported. The main strategies to reduce the risk of contamination will also be recommended.

2021 ◽  
pp. 239-244
Author(s):  
Fabrizio Antolini ◽  
Antonio Giusti

Tourism is a very important economic activity for many nations and Italy is among those that particularly benefit from it. In fact, even during the period of pandemic, despite the crisis, tourism in Italy proved to be a particularly resilient sector: among all European countries, Italy is the one that recorded the highest number of total tourist nights-spent. However, tourism statistics are not yet exhaustive in describing a highly variable phenomenon at the territorial level. Even the tourism satellite accounts, so useful for sectoral planning at regional level, are compiled for the whole country. Instead, the territories do not always know themselves, while the enhancement of landscape resources is one of the major issues that has not always been adequately analyzed; also in the recently approved Italian PNRR/Next Generation EU. In this article, we aim to examine the trend of the tourism phenomenon in the various Italian regions over the past 15 years; a period marked by crises of different origins (economic, political, health) which slowed down the economic development of the third millennium. In particular, given the problems mentioned above, we decided to start the research by considering the arrivals in hotels and non-hotel establishments by Italian tourists. The hospitality business is in fact an important part of the tourism industry. We expect domestic tourism to have greater stability, being less affected by international problems. We then examined the tourism of Italians in Italy, in the various regions, from 2006 to 2020. This analysis allowed us to observe the tourism phenomenon in Italy from a different perspective, observing, region by region, the relationship between tourism within the region and tourism coming from others regions. The choice of arrivals, instead of night spent, reduces the influence of the specific type of tourism in each region. The first results appear interesting.


2015 ◽  
Vol 19 (1) ◽  
pp. 35-60 ◽  
Author(s):  
Oana Stanciu ◽  
Roxana Banc ◽  
Anamaria Cozma ◽  
Lorena Filip ◽  
Doina Miere ◽  
...  

AbstractThe quality of cereals is very important for both human and animal nutrition. Fusarium mycotoxins include a great number of compounds. Trichothecenes, zearalenone (ZEN) and fumonisins are the major Fusarium mycotoxins occurring in cereal grains, animal feeds and forages. Conditions that predispose to mycotoxin production by Fusarium species include humidity, temperature, aeration and substrate type. Even if a great number of fungal metabolites have been designated as mycotoxins, a small number are known to have significant animal/human health and economic significance. For this, the world-wide impact of mycotoxins on human and animal health is likely underestimated and the future in this area is to identify additional specific biomarkers and group of biomarkers that can be used to establish the exposition of human and animals to individual mycotoxins.


2020 ◽  
Vol 22 (99) ◽  
pp. 182-192
Author(s):  
I. V. Yatsenko ◽  
O. I. Parilovskyi ◽  
V. M. Zhylina

The paper formulates the procedure for forensic veterinary determination of the severity of damage to animal health. According to the severity of injuries to animals, it is necessary to distinguish between injuries of three degrees: mild, moderate and severe. Forensic veterinary examination to determine the severity of injuries is carried out by a forensic veterinary expert through a veterinary examination of the injured (subject) animal. During the examination of the subject animal, the forensic veterinary expert establishes data about the animal according to the animal’s passport or other document that replaces it (animal’s nickname, individual number, species, sex, age, breed, etc.). If there are no documents for the animal, they are limited to information about the animal received from its owner or guardian; establishes the anamnestic data of the examined animal according to the explanations of the owner of the animal or its guardian; finds out the circumstances of the injury to the investigator, coroner, owner or guardian of the animal; clarifies complaints from the owner about the state of health of the animal; gets acquainted with the case materials provided by the law enforcement agency; examines other available veterinary documents, if necessary, finds out other information. The forensic veterinary expert, estimating the statute of limitations for violation of the anatomical integrity of tissues and organs and their functions, proceeds from their usual duration. Defects in veterinary care should not be taken into account when assessing the severity of injuries. In such cases, the forensic veterinary expert is obliged to indicate the nature of the causal relationship between the injury and such consequences. Damage to diseased tissues and organs of an animal can be considered as severe as damage to these organs and tissues in clinically healthy animals, if there is a direct causal link between the damage and the adverse effect. If during the examination of the subject animal the expert finds different origins of bodily injuries, he establishes what weapon of injury could have been inflicted on each of them. If the injuries are of different ages, indicate the frequency, non-simultaneity and sequence of their application, indicate the time of infliction of each of the injuries, determine the severity of each of them. In cases of death of an animal in the presence of bodily injuries, the forensic veterinary expert, along with resolving other issues, establishes the presence or absence of a causal link between the injury and the death of the animal. In cases where there is a causal link between injury and death, they can be considered fatal. All the information obtained is reflected in the expert’s report or in the report of the expert study. The results in the expert’s opinion should be the result of the analysis of objective information established during the forensic veterinary examination of the subject. They must be detailed and scientifically sound.


2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 35-36
Author(s):  
Paige N Gott ◽  
Erin F Schwandt ◽  
Shelby M Ramirez ◽  
Erika G Hendel ◽  
G Raj Murugesan ◽  
...  

Abstract Mycotoxins are secondary fungal metabolites that contaminate a variety of feedstuffs and are detrimental to animal health and productivity. The risk of broad-spectrum mycotoxin exposure is elevated for ruminants due to the complexity of their diets. This study investigated the occurrence and contamination levels of mycotoxins in forage-based feeds including corn silage, haylage (including various cuttings of multiple forage sources), and straw samples from across the US and Canada. Samples were screened for the six major mycotoxin groups: aflatoxins, type A trichothecenes, type B trichothecenes (B-Trich), fumonisins (FUM), zearalenone (ZEN), and ochratoxin A via LC-MS/MS technique. Samples submitted for clinical health or performance concerns were excluded from the data set. The B-Trich occurred most frequently (95% positive) among 92 corn silage samples harvested in 2019 followed by ZEN (34%) and FUM (9%). Mean contamination levels (parts per billion, ppb) on dry matter basis were 2,788 ppb, 456 ppb, and 194 ppb, respectively. Only 4% of silage samples were below the limit of detection (LOD) for all mycotoxins evaluated. Among 20 haylage samples from 2019 crop year, B-Trich were the most frequently detected, with 50% of samples positive at a mean concentration of 3,222 ppb. Twenty-one 2019 straw samples were submitted for analysis, with 100% testing positive for some level of B-Trich (mean 2,001 ppb) and 81% ZEN positive with an average of 640 ppb. Seventy-one percent of straw samples were positive for both B-Trich and ZEN. Preliminary mycotoxin survey results from US and Canada suggest B-Trich and ZEN are the most frequently occurring major mycotoxins in 2019 forage-based feed samples. Based on the frequency and levels of mycotoxin contamination identified in the current data set, continued analysis of forage-based feeds is warranted as mycotoxins pose a potential risk to the health, performance, and reproductive success of ruminants.


2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 172-173
Author(s):  
Erika Hendel ◽  
Shelby Ramirez ◽  
Paige Gott ◽  
G Raj Murugesan ◽  
Ursula Hofstetter

Abstract Mycotoxins are harmful secondary fungal metabolites and are of key concern to food and feed safety globally. These toxins are detrimental to animal health and can compromise animal performance even at low levels. Classic signs such as decreased feed intake and vomiting used as indicators for exposure overlook other costs of mycotoxicosis, including increasing the frequency and severity of disease via immune suppression, inciting inflammation, and modulating the gastrointestinal environment. This survey examines initial samples of the 2019 crop with previous year trends. New crop corn samples were submitted from September 2019 and consisted of corn (46%), corn silage (50%), and corn byproduct (4%). Samples were analyzed utilizing the liquid chromatography and tandem mass spectrometry (LC-MS/MS) method for six major mycotoxin groups: aflatoxins (Afla), type A trichothecenes (A-Trich), type B trichothecenes (B-Trich), fumonisins (FUM), zearalenone (ZEN), and ochratoxin-A (OTA). Data are presented for major mycotoxin classes in Table 1. Fewer samples are available thus far compared to the fall of 2018 (50 samples in 2019 vs. 135 samples in 2018), thus risk profile of this crop year is likely to change as the sample pool expands. Co-occurrence (≥ 2 mycotoxins) has decreased compared to 2018. The prevalence of B-Trich decreased compared with previous years, but levels are similar to 2018. Prevalence and levels of ZEN decreased from 2018, and are similar to 2017, while FUM is similar in prevalence to 2018, but average ppb numerically increased. As of yet, no Alfa has been detected; however, corn stored with higher moisture content has increased the risk for storage toxins. Mycotoxin risk of this harvest season is still coming into focus as harvest delays have affected sample submission. Due to continued risk of multi-mycotoxin contamination, multiple mitigation strategies are needed beyond just adsorption, including biotransformation, support of the immune system and liver function.


2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 36-37
Author(s):  
Erin F Schwandt ◽  
Paige N Gott ◽  
Erika G Hendel ◽  
Shelby M Ramirez ◽  
G Raj Murugesan ◽  
...  

Abstract Mycotoxins are secondary fungal metabolites that are detrimental to animal health and productivity. This study investigated occurrence and contamination levels of mycotoxins in the 2019 US corn harvest, including corn grain and corn byproduct feed ingredients (distillers dried grains, gluten feed, etc.). Corn samples from the 2019 harvest and corn DDGS and other byproducts from October 2019 through April 2020 were screened via the LC-MS/MS technique for the presence of six major mycotoxin groups: aflatoxins, type A trichothecenes, type B trichothecenes (B-Trich), fumonisins (FUM), zearalenone (ZEN) and ochratoxin A. Parameters of the main toxins found were compared to the two prior harvest years using the Kruskal-Wallis Test (Prism7, GraphPad, La Jolla, CA) and are presented in Table 1. Mean toxin count per sample in corn grain was similar in 2019 as 2018, with just over two toxins per sample. Mean B-Trich level (parts per billion, ppb) was elevated in 2019 from 2017. Fumonisin contamination increased in 2019 compared to 2017, and prevalence was similar to 2018. Zearalenone contamination and prevalence were similar in 2019 compared to 2018 levels. Co-contamination in corn byproduct feeds was steady, averaging nearly three toxins per sample. Mean B-Trich contamination was higher than the 2017 crop, and FUM contamination was higher in 2019 than 2017 and 2018. Zearalenone levels in 2019 byproducts are the highest seen in the past three years. Preliminary results of the 2019 corn-based feed ingredients survey suggest mycotoxin occurrence and contamination levels are approaching those observed in the challenging 2018 crop. Furthermore, there are continued concerns for co-contamination and greater ZEN contamination that may impose negative effects on growth, health and reproductive performance in ruminants.


2003 ◽  
Vol 57 (3-4) ◽  
pp. 191-199 ◽  
Author(s):  
Zoran Masic ◽  
Milan Adamovic ◽  
Sandra Djilas ◽  
Zeljko Mihaljev

Depending on the age and production category, cattle show different sensitivity towards certain mycotoxins. Microflora of the rumen degrades to a different degree and inactivates mycotoxins. In the work are presented the most important mycotoxicoses of cattle caused by fungal metabolites from the genera Fusarium, Aspergillus and Penicillium. Poisoning of cattle in our area is most often caused by Zearalenone, Dioxinivalenol, T-2 toxin, Ochratoxin A and Aflatoxin, but in the work are also presented Fumonisin B1 and B2. The work also describes preventive possibilities and protection of animal health from the effects of mycotoxins.


2018 ◽  
Vol 72 (1) ◽  
pp. 14-21
Author(s):  
Dragoljub Jovanovic ◽  
Radmila Markovic ◽  
Stamen Radulovic ◽  
Svetlana Grdovic ◽  
Milena Krstic ◽  
...  

Background. Mycotoxins are toxic secondary metabolites, which are synthesized from a large number of intermediates from the primary metabolism of saprophytic molds. Aflatoxins, due to their genotoxic and carcinogenic effects, are the most important group of mycotoxins from the aspect of their impact on human and animal health. Of all known aflatoxins, AFB1 is the most frequent, with the most harmful impact on human and animal health. Scope and Approach. Due to their prevalence and toxicity, monitoring the presence of aflatoxins in the food chain is required. The scope of this paper is to provide information on the presence of aflatoxins in animal feed and in milk. This paper describes temperature increases in Europe that are contributing to the increased presence of aflatoxins in food, as well as aflatoxin prevention and protection measures. Key Findings and Conclusions. During the last decade, serious contamination of corn with aflatoxins was recorded in southern Europe. In the summer of 2012, Serbia recorded high concentrations of aflatoxins in corn and milk. Based on climate change data, it is expected that aflatoxin contamination in corn will become more frequent.


Biomolecules ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 295
Author(s):  
Marco Masi ◽  
Mariagioia Petraretti ◽  
Antonino De Natale ◽  
Antonino Pollio ◽  
Antonio Evidente

Fungi are among the biotic agents that can cause deterioration of building stones and cultural heritage. The most common methods used to control fungal spread and growth are based on chemical pesticides. However, the massive use of these synthetic chemicals produces heavy environmental pollution and risk to human and animal health. Furthermore, their use is time dependent and relies on the repetition of treatments, which increases the possibility of altering building stones and culture heritage through environmental contamination. One alternative is the use of natural products with high antifungal activity, which can result in reduced toxicity and deterioration of archeological remains. Recently, three fungal strains, namely Aspergillus niger, Alternaria alternata and Fusarium oxysporum, were isolated as damaging agents from the external tuff wall of the Roman remains “Villa of Poppea” in Oplontis, Naples, Italy. In this manuscript, three selected fungal metabolites, namely cyclopaldic acid, cavoxin and epi-epoformin, produced by fungi pathogenic for forest plants, were evaluated as potential antifungal compounds against the above fungi. Cavoxin and epi-epoformin showed antifungal activity against Asperigillus niger and Fusarium oxysporum, while cyclopaldic acid showed no activity when tested on the three fungi. The same antifungal activity was observed in vitro experiments on infected stones of the Neapolitan yellow tuff (NYT), a volcanic lithotype widely diffused in the archeological sites of Campania, Italy. This study represents a first step in the use of these two fungal metabolites to allow better preservation of artworks and to guarantee the conditions suitable for their conservation.


Author(s):  
Verica Juric ◽  
Igor Jajic ◽  
Tatjana Savkovic ◽  
Biljana Abramovic ◽  
Milutin Ristic ◽  
...  

HACCP (Hazard Analysis and Critical Control Point) is a concept which identifies, evaluates, and controls hazards, significant for food safety. It is applicable to the entire food chain, "from farm to table". Prevention of a food borne mycotoxin contamination of commodities is the most rational and cost-effective method for preventing adverse effects of fungal metabolites on human and animal health. Deoxynivalenol (DON) belongs to the group of mycotoxins produced by certain Fusarium species, which can damage several vital organs, or demonstrate immunotoxic effect, when ingested in small amounts for a longer period of time. Of particular concern is exposure of children to this mycotoxin through cereals, which are believed to lead to reduced weight gain and decreased liver weights. For that reason, we tried to present HACCP concept for preventing wheat contamination with deoxynivalenol. To be able to apply this system, hazards must be identified and risks assessed, and for that purpose a real picture of area (region) in which preventive measures shall be applied, need to be established. According to the results of the study conducted in the laboratory at our department, DON contaminated wheat samples in the region of Vojvodina accounted for 41,6% in 2004 and 2005, whereas amounts of deoxynivalenol ranged from 57 to 1840 ?g/kg.


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