Evaluation of the Antimicrobial Activity of Olive and Rosemary Leave Extracts Prepared with Different Solvents Against Antibiotic-Resistant Escherichia coli

Background: This study was done to investigate the antimicrobial activity of rosemary and olive extracts on antibiotic-resistant Escherichia coli isolated from quail feces in Zabol city. Methods: Ethanolic and methanolic extracts of rosemary (leaf) and olive (leaf) plants were prepared using a rotary apparatus. Also, E. coli strains were isolated from poultry feces samples, the minimum inhibitory concentration and the minimum bactericidal concentration were determined by the microdilution method. Results: The lowest values of MIC and MBC against E. coli were 12.5 ppm and 25 ppm for rosemary ethanolic extract and 25 ppm and 50 ppm for rosemary methanolic extract, respectively. The lowest values of MIC and MBC against E. coli were 12.5 ppm and 25 ppm for olive ethanolic extract and 6.25 ppm and 12.5 ppm for olive methanolic extract, respectively. Conclusions: In general, methanol solvent and olive extract are highly effective against E. coli. Due to the obtained results and increasing resistance of bacteria to chemical antibiotics, it is suggested that with further studies on olives and the use of methanol solvent in the extraction of plant extracts, antibacterial compounds of olives and other plants be used in the treatment of bacterial infections.

Download Full-text

Screening of antibacterial and antifungal activities of selected Macedonian wild mushrooms

Zbornik Matice srpske za prirodne nauke ◽

10.2298/zmspn1324333n ◽

2013 ◽

pp. 333-340 ◽

Cited By ~ 8

Author(s):

Daniela Nikolovska-Nedelkoska ◽

Natalija Atanasova-Pancevska ◽

Haris Amedi ◽

Dafina Veleska ◽

Emilija Ivanova ◽

...

Keyword(s):

Antimicrobial Activity ◽

Inhibitory Concentration ◽

Methanolic Extract ◽

Antimicrobial Properties ◽

Inhibitory Effects ◽

Antibacterial And Antifungal Activities ◽

Microdilution Method ◽

Methanolic Extracts ◽

Antibacterial And Antifungal

Regarding the development of novel safe antimicrobials of natural origin, macrofungi became attractive for the researchers in the last decade. In this study, antimicrobial potential of methanolic extracts of six wild macromycetes (Boletus lupinus, Flammulina velutypes, Phellinus igniarius, Sarcodon imbricatus, Tricholoma aurantium, Xerocomus ichnusanus) was evaluated. In vitro antimicrobial activity was investigated by the microdilution method and minimum inhibitory concentration (MIC) was determined. Testing was conducted against eleven microorganisms, including six strains of bacteria and five species of fungi. Extracts showed selective antimicrobial properties while the activities depended both on the species of microorganism and on the type and concentration of extract. The evaluated extracts demonstrated antimicrobial activity, exhibiting more potent inhibitory effects on the growth of bacteria than on fungi. The highest antibacterial and antifungal activity was observed in methanolic extract of polypore fungus P. igniarius.

Download Full-text

Investigating the antimicrobial activity of extract prepared by ultrasound against Escherichia coli isolated from poultry stool

Pharmaceutics and Pharmacology Research ◽

10.31579/2693-7247/018 ◽

2020 ◽

Vol 3 (2) ◽

pp. 01-05

Author(s):

Elham Javadian

Keyword(s):

Escherichia Coli ◽

Antimicrobial Activity ◽

Minimum Inhibitory Concentration ◽

Withania Somnifera ◽

Inhibitory Concentration ◽

Rosmarinus Officinalis ◽

Mentha Piperita ◽

Antibiotic Resistant ◽

Rotary Machine ◽

Microdilution Method

Background: The aim of this study was to investigate the antimicrobial activity of Mentha piperita , Withania somnifera and Rosmarinus officinalis extract on antibiotic resistant Escherichia coli isolated from poultry stool in Zabol city. Materials & Methods: The extract of Mentha piperita, Rosmarinus officinalis and Withania somnifera was prepared by rotary machine. Escherichia coli strains were isolated from poultry stool samples. Minimum inhibitory concentration and minimum bactericidal concentration were determined by microdilution method. Results: The results of this study showed that Mentha piperita extract showed the highest inhibitory ratio compared to other extracts. The minimum inhibitory concentration was 3.1 ppm, with 5 strains inhibited at this concentration. Discussion: The results of this study could be an effective strategy for production and preparation of medicinal plants to help treating E. coli infections.

Download Full-text

PRELIMINARY PHYTOCHEMICAL AND ANTIMICROBIAL SCREENING OF UDHARA VAYUHARA CHURNA

INDIAN DRUGS ◽

10.53879/id.58.06.11787 ◽

2021 ◽

Vol 58 (06) ◽

pp. 64-67

Author(s):

Maruthi T. Ekbote ◽

◽

Rajashekar K. V ◽

Shankarappa L ◽

Bharathi D. R. ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Methanolic Extract ◽

Acorus Calamus ◽

Aqueous Extracts ◽

Piper Longum ◽

Polyherbal Formulation ◽

Methanolic Extracts ◽

Phytochemical Investigation

Ayurvedic medicines are being used increasingly to fight or prevent common diseases. Udhara vayuhara churna is a polyherbal formulation containing Piper longum (Piperceae) Zingiber officinalis (Zingiberaceae), Elettaria cardamom (Zingiberaceae), Plumbago zeylanica (Plumbaginaceae), Carum carvi (Umbelliferae), Acorus calamus (Araceae) and Embilica officinalis (Euphorbiaceae). Methanolic and aqueous extracts of Udhara vayuhara churna were screened for their antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Aspergillus niger and Candida albicans. The results of antimicrobial activity of aqueous and methanolic extracts of the Udhara vayuhara churna indicated that methanolic extract inhibited the growth of one or more test pathogens than aqueous extract. Udhara vayuhara churna formulation extracts showed a broad spectrum of antimicrobial activity. Phytochemical investigation revealed the presence of tannins, saponins, alkaloids, glycosides, flavonoids and essential oils. This may be due to the multifunctional effect of all the seven plant ingredients of Udhara Vayuhara churn.

Download Full-text

ANTIMICROBIAL ACTIVITY OF AQUEOUS AND ETHANOLIC LEAF EXTRACTS OF ANACARDIUM OCCIDENTALE

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i12.29073 ◽

2018 ◽

Vol 11 (12) ◽

pp. 474

Author(s):

Shobha Kl ◽

Amita Shobha Rao ◽

Pai Ksr ◽

Sujatha Bhat

Keyword(s):

Escherichia Coli ◽

Antimicrobial Activity ◽

Klebsiella Pneumoniae ◽

Bacterial Infections ◽

Ethanolic Extract ◽

Culture Collection ◽

Anacardium Occidentale ◽

Diffusion Method ◽

Leaf Extracts

Objective: The objective of this study was to evaluate the antimicrobial activity of leaves of Anacardium occidentale (A. occidentale) against microorganisms including multidrug-resistant (MDR) bacteria. Methods: Agar well diffusion method was employed to demonstrate the antimicrobial activity of leaves A. occidentale. Ethanol and aqueous extracts of the leaves were used against microorganisms, which included American type culture collection strains of Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Enterococcus faecalis, and Pseudomonas aeruginosa and the clinical isolates of Streptococcus pneumoniae, Candida albicans, MDR Escherichia coli, and MDR Klebsiella pneumoniae. Results: The ethanolic extract of leaves of A. occidentale showed significant antimicrobial activity. Aqueous extract had mild antifungal activity. Conclusion: Ethanolic extract of leaves of A. occidentale could be a good source for the antibacterials to combat MDR bacterial infections. Further studies are necessary for these potent plant extracts to evaluate the in vivo efficacy and toxicity.

Download Full-text

Synergism of the Combination of Traditional Antibiotics and Novel Phenolic Compounds against Escherichia coli

Pathogens ◽

10.3390/pathogens9100811 ◽

2020 ◽

Vol 9 (10) ◽

pp. 811

Author(s):

Md. Akil Hossain ◽

Hae-Chul Park ◽

Sung-Won Park ◽

Seung-Chun Park ◽

Min-Goo Seo ◽

...

Keyword(s):

Escherichia Coli ◽

Rattus Norvegicus ◽

Inhibitory Concentration ◽

Epicatechin Gallate ◽

Antibiotic Resistant ◽

E Coli ◽

Pathogenic Escherichia Coli ◽

Resistant Strains ◽

Novel Approaches ◽

Rapid Emergence

Pathogenic Escherichia coli (E. coli)-associated infections are becoming difficult to treat because of the rapid emergence of antibiotic-resistant strains. Novel approaches are required to prevent the progression of resistance and to extend the lifespan of existing antibiotics. This study was designed to improve the effectiveness of traditional antibiotics against E. coli using a combination of the gallic acid (GA), hamamelitannin, epicatechin gallate, epigallocatechin, and epicatechin. The fractional inhibitory concentration index (FICI) of each of the phenolic compound-antibiotic combinations against E. coli was ascertained. Considering the clinical significance and FICI, two combinations (hamamelitannin-erythromycin and GA-ampicillin) were evaluated for their impact on certain virulence factors of E. coli. Finally, the effects of hamamelitannin and GA on Rattus norvegicus (IEC-6) cell viability were investigated. The FICIs of the antibacterial combinations against E. coli were 0.281–1.008. The GA-ampicillin and hamamelitannin-erythromycin combinations more effectively prohibited the growth, biofilm viability, and swim and swarm motilities of E. coli than individual antibiotics. The concentration of hamamelitannin and GA required to reduce viability by 50% (IC50) in IEC-6 cells was 988.54 μM and 564.55 μM, correspondingly. GA-ampicillin and hamamelitannin-erythromycin may be potent combinations and promising candidates for eradicating pathogenic E. coli in humans and animals.

Download Full-text

Antimicrobial Activity of Opuntia cochenillifera (L.) Mill Fruit and Cladode Extracts

International Journal of Pharmacology Phytochemistry and Ethnomedicine ◽

10.18052/www.scipress.com/ijppe.3.84 ◽

2016 ◽

Vol 3 ◽

pp. 84-89

Author(s):

Pooja Suryawanshi ◽

G.M. Vidyasagar

Keyword(s):

Antimicrobial Activity ◽

Minimum Inhibitory Concentration ◽

Inhibitory Concentration ◽

Methanolic Extract ◽

Maximum Activity ◽

Methanolic Extracts ◽

Agar Well Diffusion Assay ◽

Diffusion Assay

In the present study the antimicrobial activity of chloroform and methanolic extracts of Opuntiacochenillifera for both cladode and fruits was investigated. Methanolic extract was found to be an effective against the microbes namely, E. coli,Bacillus subtilis,Staphylococcus aureus, Pseudomonas aeruginosaCandidaalbicanC.glabrataC.haemuloniiC.Tropicalis. Maximum activity was observed against E. coli, B. Subtilisand,C.albican and C.glabrata at 40mg/ml. Agar well diffusion assay was used to determine minimum inhibitory concentration of all test microorganisms.

Download Full-text

ANTIMICROBIAL ACTIVITY OF METHANOLIC EXTRACT OF LUDWIGIA PARVIFLORA L. AGAINST STANDARD BACTERIAL STRAINS AND COMPARISON OF ITS ACTIVITY WITH THAT OF STANDARD ANTIBIOTICS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i3.30818 ◽

2019 ◽

pp. 430-432

Author(s):

SHIBU GEORGE ◽

MEVLIN JOY

Keyword(s):

Antimicrobial Activity ◽

Bacterial Infections ◽

Methanolic Extract ◽

Diffusion Method ◽

Bacterial Strains ◽

Disk Diffusion Method ◽

E Coli ◽

Whole Plant ◽

The Common ◽

Susceptibility Tests

Objective: The objective of this study was to evaluate the antimicrobial activity of methanolic extract of Ludwigia parviflora L. using standard bacterial strains and compare its activity with that of standard antibiotics. Methods: The antibacterial activity and antibiotic susceptibility tests were done by disk diffusion method using MTCC bacterial strains. Results: The study revealed that the methanolic extract of the whole plant of L. parviflora L. was effective to inhibit the growth of Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Escherichia coli. Among the tested strains, S. aureus, P. aeruginosa, K. pneumoniae, and E. coli were more susceptible to the methanolic extract of L. parviflora than the commonly using antibiotic tetracycline 30 mcg. The activity of methanolic extract was also higher than the activity of gentamicin 10 mcg against the P. aeruginosa. Conclusion: The study concluded that the crude methanolic extract of the whole plant of L. parviflora L. is a good source for antibacterial agent against S. aureus, P. aeruginosa, K. pneumoniae, and E. coli. Hence, this plant can be used as a natural alternative to the common antibiotics such as gentamicin and tetracycline against common bacterial infections after validating its pharmacological and toxicological activities.

Download Full-text

Evaluation of the antibacterial and modulatory activities of ethanolic excract of Calotropis procera (Aiton) W.T. Aiton against multiresistant bacterial strains

Anales de Biología ◽

10.6018/analesbio.43.19 ◽

2021 ◽

pp. 205-209

Author(s):

Yuri Geraldo ◽

Livia Leandro ◽

Ana Raquel Silva ◽

Fábia Campina ◽

Ana Carolina Araújo ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Inhibitory Concentration ◽

Ethanolic Extract ◽

Calotropis Procera ◽

Subinhibitory Concentration ◽

Bacterial Strains ◽

Resistant Species ◽

Microdilution Method

The objective of this study was to evaluate the antibacterial and modulatory activities of ethanolic extract of Calotropis procera (Aiton) W.T. Aiton against resistant species. By microdilution method, the minimum inhibitory concentration (MIC) of the extract and modulation of the subinhibitory concentration MIC/8 to norfloxacine, gentamicin and imipenem against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. There was obtained 512 μg/mL to Pseudomonas aeruginosa. To Staphylococcus aureus, modulation showed synergism to norfloxacin and gentamicin, with imipenem against Pseudomonas aeruginosa and gentamicin against Escherichia coli. Based on these results, more studies are needed to test the antibacterial activity of the extract. El objetivo de este estudio fue evaluar la actividad antibacteriana y moduladora del extracto etanólico de Calotropis procera (Aiton) W.T. Aiton contra cepas multirresistentes de bacterias. Por el método de microdilución, fueron definidas la concentración inhibidora mínima (MIC) del extracto y la modulación con la concentración inhibidora CIM / 8 del extracto con norfloxacina, gentamicina e imipenem contra Staphylococcus aureus, Escherichia coli y Pseudomonas aeruginosa. Se obtuvo 512 μg/mL para Pseudomonas aeruginosa. Se descubrió sinergismo en el caso de Staphylococcus aureus, en la modulación con norfloxacina y gentamicina, mientras que con imipenem frente a Pseudomonas aeruginosa y con gentamicina para Escherichia coli. Con base en estos resultados, se necesitan más estudios para probar la actividad antibacteriana del extracto.

Download Full-text

Biological Properties of Structurally Related α-Helical Cationic Antimicrobial Peptides

Infection and Immunity ◽

10.1128/iai.67.4.2005-2009.1999 ◽

1999 ◽

Vol 67 (4) ◽

pp. 2005-2009 ◽

Cited By ~ 103

Author(s):

Monisha G. Scott ◽

Hong Yan ◽

Robert E. W. Hancock

Keyword(s):

Escherichia Coli ◽

Antimicrobial Activity ◽

Tumor Necrosis ◽

Biological Properties ◽

Cationic Antimicrobial Peptide ◽

Antibiotic Resistant ◽

E Coli ◽

Resistant Strains ◽

Conventional Antibiotics ◽

Necrosis Factor

ABSTRACT A series of α-helical cationic antimicrobial peptide variants with small amino acid changes was designed. Alterations in the charge, hydrophobicity, or length of the variant peptides did not improve the antimicrobial activity, and there was no statistically significant correlation between any of these factors and the MIC forPseudomonas aeruginosa, Escherichia coli, orSalmonella typhimurium. Individual peptides demonstrated synergy with conventional antibiotics against antibiotic-resistant strains of P. aeruginosa. The peptides varied considerably in the ability to bind E. coli O111:B4 lipopolysaccharide (LPS), and this correlated significantly with their antimicrobial activity and ability to block LPS-stimulated tumor necrosis factor and interleukin-6 production. In general, the peptides studied here demonstrated a broad range of activities, including antimicrobial, antiendotoxin, and enhancer activities.

Download Full-text

Phytochemical Extracts of Leucas Aspera and Dahlia Pinnata Exhibit Antimicrobial Properties on Escherichia Coli and Enterococcus Faecalis.

Current Biotechnology ◽

10.2174/2211550109999201027201433 ◽

2020 ◽

Vol 09 ◽

Author(s):

Yamuna Saravanan ◽

Bala Subramaniam Devaraj ◽

Nithesh Kumar Velusamy ◽

Pooja Shree Soundirarajan ◽

Kumaravel Kandaswamy

Keyword(s):

Escherichia Coli ◽

Antimicrobial Activity ◽

Enterococcus Faecalis ◽

Bacterial Infections ◽

Aqueous Extracts ◽

Bacterial Strains ◽

Efficacy Evaluation ◽

E Coli ◽

Methanol Extracts ◽

Leucas Aspera

Background:: Phytochemicals such as tannins, alkaloids, flavonoids, and peptides been found to have antimicrobial activity against a variety of bacterial strains. Objective:: However, optimal extraction procedures for these phytochemicals and their efficacy evaluation against certain pathogenic bacterial strains remain unclear. Method:: Therefore, in this study phytochemicals from Leucas aspera (L. aspera) and Dahlia pinnata (D. pinnata) were extracted by hot and cold extraction methods using water and methanol as solvents. In addition, antimicrobial activity of L. aspera and D. pinnata extracts against bacterial strains such as the gram-negative Escherichia coli (E. coli) and the gram-positive Enterococcus faecalis (E. faecalis) were performed by Minimal Inhibitory Concentration (MIC) and CFU quantification assays. Results:: The majority of the phytochemicals such as protein, carbohydrate, tannins, flavonoids, phenols, and saponins were present in our extracts but steroids were absent in the extract. Protein, tannins, flavonoids, phenols, and saponins were present in both L. aspera and D. pinnata. The yield of proteins was high (1.990 ± 0.091 mg/ml) in methanol extracts of L. aspera and low (0.199 mg/ml) in aqueous extracts. However, the yield of tannins was high (1.713 ± 0.079 mg/ml) in methanol extracts of D. pinnata and low (0.528 ± 0.136 mg/ml) aqueous extracts. The MIC of D. pinnata extracts were found to be 200 mg/ml for both E. coli and E. faecalis. However the L. aspera extracts had a MIC of 100 mg/ml and 200 mg/ml on E. coli and E. faecalis, respectively. Conclusion:: This article demonstrated the potential use of phytochemicals as novel antimicrobial compounds against bacterial infections.

Download Full-text