scholarly journals ANTIMICROBIAL ACTIVITY OF METHANOLIC EXTRACT OF LUDWIGIA PARVIFLORA L. AGAINST STANDARD BACTERIAL STRAINS AND COMPARISON OF ITS ACTIVITY WITH THAT OF STANDARD ANTIBIOTICS

2019 ◽  
pp. 430-432
Author(s):  
SHIBU GEORGE ◽  
MEVLIN JOY
Keyword(s):  
Antimicrobial Activity ◽  
Bacterial Infections ◽  
Methanolic Extract ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Whole Plant ◽  
The Common ◽  
Susceptibility Tests

Objective: The objective of this study was to evaluate the antimicrobial activity of methanolic extract of Ludwigia parviflora L. using standard bacterial strains and compare its activity with that of standard antibiotics. Methods: The antibacterial activity and antibiotic susceptibility tests were done by disk diffusion method using MTCC bacterial strains. Results: The study revealed that the methanolic extract of the whole plant of L. parviflora L. was effective to inhibit the growth of Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Escherichia coli. Among the tested strains, S. aureus, P. aeruginosa, K. pneumoniae, and E. coli were more susceptible to the methanolic extract of L. parviflora than the commonly using antibiotic tetracycline 30 mcg. The activity of methanolic extract was also higher than the activity of gentamicin 10 mcg against the P. aeruginosa. Conclusion: The study concluded that the crude methanolic extract of the whole plant of L. parviflora L. is a good source for antibacterial agent against S. aureus, P. aeruginosa, K. pneumoniae, and E. coli. Hence, this plant can be used as a natural alternative to the common antibiotics such as gentamicin and tetracycline against common bacterial infections after validating its pharmacological and toxicological activities.

SEARCH FOR ANTIMICROBIAL AGENTS: QUINOXALINE AS A PROMISING TARGET

INDIAN DRUGS ◽  
2017 ◽  
Vol 54 (07) ◽  
pp. 5-9
Author(s):  
R. G Ingle ◽  
◽  
S. J. Wadher
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Agents ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Disk Diffusion Method ◽  
Gram Negative ◽  
E Coli ◽  
Promising Target ◽  
Quinoxaline Derivatives

A set of ten new 2,3-diphenyl-6-sulfonamido quinoxaline derivatives was synthesized and screened for antimicrobial activity by disk diffusion method. Test derivatives R3, R5, R’’1 and R’’2 show promising results against bacterial strains S. aureus gram positive and E. coli gram negative organism with the concentration 1000 μg/mL in disk diffusion method. Rest of the derivatives show sensitivity against the same organisms. All the synthesized derivatives were confirmed by their spectral data.

scholarly journals Study of the in vitro Anti-bacterial Susceptibility of the Commercially Available Antibiotics in Suspension or Solution Forms Collected within Dhaka Metropolis

2020 ◽  
Vol 36 (2) ◽  
pp. 79-83
Author(s):  
Asma Akter Bhuiyan ◽  
Mehran Mosharraff Hossain Niloy ◽  
Anamika Chakrabarty ◽  
Syeda Muntaka Maniha ◽  
Rashed Noor
Keyword(s):  
Bacterial Infections ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
E Coli ◽  
Antibiotic Solution ◽  
Test Organisms ◽  
Antibacterial Susceptibility ◽  
Susceptibility Tests ◽  
The City

Consumption of a range of antibiotics upon bacterial infections is a common chemotherapeutic practice. Current study attempted to detect the in vitro anti-bacterial activities of such finished medicaments against some of the selected bacterial strains in order to examine their sustainable effectiveness against microorganisms. A total of six categories of samples were randomly collected from different pharmacies within the city of Dhaka. The antibacterial susceptibility tests were conducted employing the Kirby-Bauer (agar well diffusion) method. The test bacterial strains used in this study were Escherichia coli, Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa and P. putida and P. fluorescence. While E. coli was found to be sensitive towards all the antibiotics used, others showed resistance to a significant extent. flucloxacillin, cephradine and salbutamol were noticed to be completely inactive against Pseudomonas species. Besides, the salbutamol was found only to suppress the growth of E. coli but the other five test organisms were completely resistant against this antibiotic solution. Bangladesh J Microbiol, Volume 36 Number 2 December 2019, pp 79-83

scholarly journals STUDY OF VARIOUS FIVE EXTRACTS OF TRACHYSPERMUM AMMI SEEDS ON SELECTED FOUR GRAM-NEGATIVE COOKED FOOD SPOILAGE BACTERIAL STRAINS BY DISK DIFFUSION METHOD

2015 ◽  
Vol 3 (9SE) ◽  
pp. 1-3
Author(s):  
Usha Masih
Keyword(s):  
Antimicrobial Activity ◽  
Cold Water ◽  
Bacterial Species ◽  
Antibacterial Properties ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Disk Diffusion Method ◽  
Gram Negative ◽  
E Coli ◽  
Trachyspermum Ammi

Bacterial foodborne diseases are caused by consumption of foods contaminated with bacteria or their toxins. This study evaluated antibacterial properties of Trachyspermum ammi Acetone, ethanol, methanol, cold water and hard water extracts of spice (Ajowan) seeds against four gram negative strains of pathogenic foodborne bacteria, E. coli O157:H7   ATCC 43888, ATCC 25922, ATCC 8739 and ATCC 43895 that cause infection and intoxication. E. coli ATCC 43888 was observed to be highly susceptible to all extracts of ajowan with absolute zones of inhibition in the range of 16mm – 23mm in diameter. This study demonstrated that spice extracts have antimicrobial activity against food-borne bacterial species and should be considered as potential antibacterial agents for addition to ready meals. The spice contain high amount of secondary metabolites due to these metabolites they have high antimicrobial activity and it can be used as good bio- preserver and it can also use for medicinal purpose.

scholarly journals Comparative Study on the Antimicrobial Efficacy of Methanolic Extract of Root, Callus and Fruit Extracts of Myxopyrum smilacifolium Blume

2014 ◽  
Vol 2 (4) ◽  
pp. 521-524
Author(s):  
RP Praveen ◽  
Ashalantha Nair
Keyword(s):  
Antimicrobial Activity ◽  
Methanolic Extract ◽  
Fungal Strain ◽  
Antimicrobial Efficacy ◽  
Bacterial Strains ◽  
E Coli ◽  
Root Extracts ◽  
Fruit Extracts ◽  
Root Callus ◽  
And Staphylococcus Aureus

The aim of the present study was to compare the antimicrobial efficacy of methanolic extract of root, callus and fruit of Myxopyrum smilacifolium Blume. Antimicrobial activity was tested using agar well diffusion with four bacterial strains viz: Escherechia coli, Enterococcus faecalis, Bacillus subtilis and Staphylococcus aureus of which E. coli alone was gram negative. The fungal strain employed was Candida albicans. Root extracts shown to be effective only against B. subtilis. Fruit extracts showed the maximum antimicrobial activity against all the microbial species considered for the current study except against S. aureus. Highlight of the present study was the antimicrobial activity of callus extracts. DOI: http://dx.doi.org/10.3126/ijasbt.v2i4.11362  Int J Appl Sci Biotechnol, Vol. 2(4): 521-524 

Trigona laeviceps Propolis from Thailand: Antimicrobial, Antiproliferative and Cytotoxic Activities

2009 ◽  
Vol 37 (05) ◽  
pp. 855-865 ◽  
Author(s):  
Supawadee Umthong ◽  
Songchan Puthong ◽  
Chanpen Chanchao
Keyword(s):  
Antimicrobial Activity ◽  
Methanolic Extract ◽  
Water Extract ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Cytotoxic Activities ◽  
E Coli ◽  
Anticancer Properties ◽  
Crude Extracts ◽  
Sw620 Cells

Propolis is one of the natural bee products which has long been used as a crude preventative and prophylactic medicine, and has been reported to possess antibacterial, antiviral, anti-inflammatory, antioxidative and anticancer properties. Propolis of the stingless bee, Trigona laeviceps, was extracted by water or methanol at 35% (w/v) yielding a crude water or a methanolic extract at 60 and 80 mg/ml, respectively, which is 17.1 and 22.9% (w/w) of the total propolis, respectively. The antimicrobial activity of both crude extracts was assayed on four selected pathogenic microbes by using the agar well diffusion method. The results suggested that both water and methanolic crude extracts have some antimicrobial activities, water extract has greater antimicrobial activity than methanolic extract. The relative order of sensitivity of the four microbes were, however, the same between the two extracts from the most to least sensitive, S. aureus > E. coli ≫ C. albicans ⋙ A. niger, with indeed no observed growth inhibition of A. niger at all. Antiproliferative and cytotoxic affects were tested on the colon carcinoma cell line, SW620, using the three parameters: (1) MTT assay; (2) cell morphology; and (3) the fragmentation of genomic DNA. The water extract of propolis showed a higher antiproliferative activity than that of methanolic extract to SW620 cells, additionally both appeared to cause cell death by necrosis.

Antimicrobial activity of raw soybean, soybean flour and roasted soybean extracted by ethanol-hexane method

2017 ◽  
Vol 119 (10) ◽  
pp. 2277-2286 ◽  
Author(s):  
Sireerat Laodheerasiri ◽  
Nirasha Horana Pathirage
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Antimicrobial Compounds ◽  
Soybean Flour ◽  
Hexane Extraction ◽  
Disk Diffusion Method ◽  
Content Type ◽  
E Coli ◽  
Inhibitory Compounds

Purpose The purpose of this paper is to determine the antimicrobial compounds extracted from various types of soybean materials; raw soybean, soybean flour and roasted soybean. Bacteria growth inhibition was tested in E.coli and S. aureus by disk diffusion method. Efficiency of antimicrobial activities were compared among the extracted solution. Design/methodology/approach Soybean (Glycine max) isoflavones contain the ability to inhibit the growth of many strains of microorganisms. The solid-liquid extraction, ethanol-hexane extraction, was modified to isolate the inhibitory compounds from the three different types of soybean materials. All crude extracts at various concentrations performed under different extracted solutions (75, 50, 25, 12.5, 6.25 and 3.125 percent) were tested with E. coli. and S. aureus to determine the antimicrobial activities and the minimum inhibition concentration (MIC). Disk diffusion method was chosen to study the antimicrobial activity of isoflavones. Findings Soybean flour extract inhibited the growth of E. coli and S. aureus more effective than roasted soybean and raw soybean, respectively. The MIC of inhibitory compounds extracted from soybean flour was 6.25 percent (0.031 mg/ml), roasted soybean was 25 percent (0.125 mg/ml) and raw soybean was 50 percent (0.250 mg/ml). Crude extracts of soybean flour and roasted soybean showed better results than raw soybean. All the three of extracted soybeans inhibited the growth of E. coli better than S. aureus. Originality/value Ethanol-hexane extraction was successfully used to isolate the antimicrobial compounds from raw soybean, soybean flour and roasted soybean. Comparison of antimicrobial activity showed that soybean flour contained the highest inhibition activity.

scholarly journals PHYTOCHEMICAL SCREENING AND ANTIMICROBIAL ACTIVITY OF BLUMEA MOLLIS (D.DON) MERRILL

2019 ◽  
pp. 142-145
Author(s):  
JYOTHILEKSHMY V ◽  
ANJALI NAIR G ◽  
VYSHAK K ◽  
RADHIKA R NAIR ◽  
AMRITA MURALIKRISHNAN ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Phenolic Acids ◽  
Methanolic Extract ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Whole Plant ◽  
Analytical Response ◽  
Antibacterial And Antifungal ◽  
Chloroform Methanol ◽  
Gallic Acid Equivalent

Objective: The objective of this research was to carry out the preliminary phytochemical screening and to evaluate the antimicrobial activity of the methanolic extract of the Blumea mollis. Methods: In the present work, the phytochemicals of the whole plant were extracted using petroleum ether, chloroform, methanol, ethanol, and distilled water. These were then screened for triterpenes/steroids, alkaloids, anthraquinones, coumarins, flavonoids, saponins, tannins, and phenolic acids using standard methods. Further, the antimicrobial activity of the methanolic extract was determined using Gram-positive and negative bacteria. Agar well diffusion method was employed for antimicrobial activity study, and the zone of inhibition was found out. Results: The intensity of the color produced or the precipitate formed was used as the analytical response to these tests. Flavonoids, tannins, and phenolic acids showed positive results for phytochemical screening. The concentration of total phenols and flavonoids of the decoction was 45.5±0.2000 μg/ml gallic acid equivalent and 42.3±0.3606 μg/ml quercetin equivalent, respectively. Staphylococcus aureus, Escherichia coli, and Candida albicans were inhibited by the methanolic extract of B. mollis in agar well diffusion test. Conclusion: The results of the present study showed that the methanolic extract of B. mollis contains glycosides, phenolics, tannins, and flavonoids. Plant possesses significant antibacterial and antifungal property; the potent antimicrobial activity of the plant could be attributed to the presence of flavonoids and phenolic compounds in it.

Phytochemical Extracts of Leucas Aspera and Dahlia Pinnata Exhibit Antimicrobial Properties on Escherichia Coli and Enterococcus Faecalis.

2020 ◽  
Vol 09 ◽  
Author(s):  
Yamuna Saravanan ◽  
Bala Subramaniam Devaraj ◽  
Nithesh Kumar Velusamy ◽  
Pooja Shree Soundirarajan ◽  
Kumaravel Kandaswamy
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Enterococcus Faecalis ◽  
Bacterial Infections ◽  
Aqueous Extracts ◽  
Bacterial Strains ◽  
Efficacy Evaluation ◽  
E Coli ◽  
Methanol Extracts ◽  
Leucas Aspera

Background:: Phytochemicals such as tannins, alkaloids, flavonoids, and peptides been found to have antimicrobial activity against a variety of bacterial strains. Objective:: However, optimal extraction procedures for these phytochemicals and their efficacy evaluation against certain pathogenic bacterial strains remain unclear. Method:: Therefore, in this study phytochemicals from Leucas aspera (L. aspera) and Dahlia pinnata (D. pinnata) were extracted by hot and cold extraction methods using water and methanol as solvents. In addition, antimicrobial activity of L. aspera and D. pinnata extracts against bacterial strains such as the gram-negative Escherichia coli (E. coli) and the gram-positive Enterococcus faecalis (E. faecalis) were performed by Minimal Inhibitory Concentration (MIC) and CFU quantification assays. Results:: The majority of the phytochemicals such as protein, carbohydrate, tannins, flavonoids, phenols, and saponins were present in our extracts but steroids were absent in the extract. Protein, tannins, flavonoids, phenols, and saponins were present in both L. aspera and D. pinnata. The yield of proteins was high (1.990 ± 0.091 mg/ml) in methanol extracts of L. aspera and low (0.199 mg/ml) in aqueous extracts. However, the yield of tannins was high (1.713 ± 0.079 mg/ml) in methanol extracts of D. pinnata and low (0.528 ± 0.136 mg/ml) aqueous extracts. The MIC of D. pinnata extracts were found to be 200 mg/ml for both E. coli and E. faecalis. However the L. aspera extracts had a MIC of 100 mg/ml and 200 mg/ml on E. coli and E. faecalis, respectively. Conclusion:: This article demonstrated the potential use of phytochemicals as novel antimicrobial compounds against bacterial infections.

Preliminary phytochemical screening and antimicrobial activities of plant extract of Elaeocarpus ganitrus Roxb.

2016 ◽  
Vol 5 (09) ◽  
pp. 4885 ◽  
Author(s):  
Khushbu Pandey ◽  
Mahendra Singh* ◽  
Bharat Pandey ◽  
Anshulika Upadhyaya ◽  
Kamal K. Pande
Keyword(s):  
Antimicrobial Activity ◽  
Rhizopus Oryzae ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Bacterial Strains ◽  
E Coli ◽  
Antibacterial And Antifungal ◽  
Anti Fungal

The present study was carried out for phytochemical screening of principle bioactive compounds and antimicrobial activity in Elaeocarpus ganitrus Roxb., Phytochemical analysis revealed the presence of saponin, terpenoid, steroid, saponin, flavonoid, tannin and alkaloid. The petroleum, ether, chloroform, methanol, acetone and aqueous extracts were subjected to antimicrobial activity against bacterial strains Staphylococcus aureus, Pseudomonas, E. coli and Bacillus subtilis against anti-fungal strains A.awamori, A.fumigatus, Rhizopus oryzae, Trichoderma viridae and C.oryzae. The antibacterial and antifungal activity was evaluated by disc-diffusion method.

scholarly journals In vitro Synergistic Antimicrobial Activity of Romanian Propolis and Antibiotics against Escherichia coli Isolated from Bovine Mastitis

2015 ◽  
Vol 43 (2) ◽  
pp. 327-334 ◽  
Author(s):  
Mihaela NICULAE ◽  
Laura STAN ◽  
Emoke PALL ◽  
Anamaria Ioana PAȘTIU ◽  
Iulia Maria BALACI ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Bovine Mastitis ◽  
Antibacterial Properties ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Ethanolic Extracts

The study was aimed to characterize the chemical composition and the antimicrobial activity of Romanian propolis ethanolic extracts (EEP) against antibiotic-sensitive and antibiotic-resistant E. coli strains isolated from bovine mastitis. The preliminary antimicrobial screening was performed by a disk diffusion method, followed by determination of minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) based on broth microdilution assay; further, the synergistic action of propolis with antimicrobial drugs was assessed by a disk diffusion method on agar containing subinhibitory concentrations of propolis. For the chemical characterisation of EEP, the flavonoids (flavones/flavonols, flavanones/dihydroflavonols) and total phenolics were evaluated by spectrophotometric methods. The phenolic compounds of these extracts were also determined using HPLC. The results indicated for Romanian propolis ethanolic extracts the typical poplar composition profile with flavonoids and phenolic acids as main biological active compounds, with chromatographic analysis data confirmed also spectrophotometrically. In addition, positively correlated with the chemical composition, a strong antimicrobial efficacy was exhibited towards E. coli strains, along with interesting synergistic interaction with antibiotics that can be further investigated to obtain propolis-based formulation with antibacterial properties. Subsequent in vitro and in vivo studies evaluating the safety and efficacy are intended to consider propolis in veterinary therapeutic protocols.

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