scholarly journals Comparing the Effect of Two Methods of High-Intensity Interval Training (HIIT) and High-Intensity Continuous Training (HICT) on Damage and Antioxidant Indices of Liver in Male Wistar Rats

2020 ◽  
Vol 8 (1) ◽  
Author(s):  
Aghaali Ghasemnian ◽  
Mozhgan Usefpour ◽  
Ahmad Rahmani ◽  
Zeinab Iddehloei
Keyword(s):  
Superoxide Dismutase ◽  
High Intensity ◽  
Liver Tissue ◽  
Wistar Rats ◽  
Interval Training ◽  
Control Group ◽  
High Intensity Interval Training ◽  
Continuous Training ◽  
Male Wistar Rats ◽  
High Intensity Interval

Background: Nowadays, one of the widely used training methods is high-intensity interval training (HIIT). In addition, researchers have stated that long-term, high-intensity training is associated with the production of free radicals in the body. Free radicals also damage liver cells, resulting in increased liver enzymes. Objectives: The objective of this study was to investigate the effect of HIIT, compared to high-intensity continuous training (HICT), on damage and antioxidant indices of the liver in male Wistar rats. Methods: Twenty-two adult male rats were randomly divided into three groups of control (n = 6), HIIT (n = 8), and HICT (n = 8). Training protocols included HIIT and HICT on a treadmill for eight weeks (five days in a week). Standard water and food were provided for rats ad libitum. Forty-eight hours after the last training session, blood and tissue samples were collected, and serum enzymes were measured by the methods recommended by the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) method. The level of enzyme activity of superoxide dismutase in the liver tissue was measured by Randox Commercial Kit (Cat. No. SD 126). One-way analysis of variance (ANOVA) and Tukey’s post hoc tests were used to analyze the data. Results: The results showed that eight weeks of HIIT and HICT had no effect on the alanine aminotransferase (ALT) level (P = 0.14), but eight weeks of HIIT significantly decreased the serum aspartate aminotransferase (AST) level compared to the control group (P = 0.04). The level of liver superoxide dismutase (SOD) enzyme activity significantly increased in the HIIT and HICT groups compared to the control group (P = 0.001). HICT also led to a significant increase in the SOD level compared to HIIT (P = 0.03). Conclusions: The research results suggested that eight weeks of HIIT led to a significant reduction in serum AST levels, and HIIT had a lower effect on the increased SOD activity in liver tissue compared to HICT. It might be stated that HIIT is safer than HICT, and it has fewer destructive effects on liver tissue.

scholarly journals Comparing the Effect of Two Methods of High-Intensity Interval Training (HIIT) and High-Intensity Continuous Training (HICT) on Damage and Antioxidant Indices of Liver in Male Wistar Rats

2020 ◽  
Vol 8 (1) ◽  
Author(s):  
Aghaali Ghasemnian ◽  
Mozhgan Usefpour ◽  
Ahmad Rahmani ◽  
Zeinab Iddehloei
Keyword(s):  
Superoxide Dismutase ◽  
High Intensity ◽  
Liver Tissue ◽  
Wistar Rats ◽  
Interval Training ◽  
Control Group ◽  
High Intensity Interval Training ◽  
Continuous Training ◽  
Male Wistar Rats ◽  
High Intensity Interval

Background: Nowadays, one of the widely used training methods is high-intensity interval training (HIIT). In addition, researchers have stated that long-term, high-intensity training is associated with the production of free radicals in the body. Free radicals also damage liver cells, resulting in increased liver enzymes. Objectives: The objective of this study was to investigate the effect of HIIT, compared to high-intensity continuous training (HICT), on damage and antioxidant indices of the liver in male Wistar rats. Methods: Twenty-two adult male rats were randomly divided into three groups of control (n = 6), HIIT (n = 8), and HICT (n = 8). Training protocols included HIIT and HICT on a treadmill for eight weeks (five days in a week). Standard water and food were provided for rats ad libitum. Forty-eight hours after the last training session, blood and tissue samples were collected, and serum enzymes were measured by the methods recommended by the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) method. The level of enzyme activity of superoxide dismutase in the liver tissue was measured by Randox Commercial Kit (Cat. No. SD 126). One-way analysis of variance (ANOVA) and Tukey’s post hoc tests were used to analyze the data. Results: The results showed that eight weeks of HIIT and HICT had no effect on the alanine aminotransferase (ALT) level (P = 0.14), but eight weeks of HIIT significantly decreased the serum aspartate aminotransferase (AST) level compared to the control group (P = 0.04). The level of liver superoxide dismutase (SOD) enzyme activity significantly increased in the HIIT and HICT groups compared to the control group (P = 0.001). HICT also led to a significant increase in the SOD level compared to HIIT (P = 0.03). Conclusions: The research results suggested that eight weeks of HIIT led to a significant reduction in serum AST levels, and HIIT had a lower effect on the increased SOD activity in liver tissue compared to HICT. It might be stated that HIIT is safer than HICT, and it has fewer destructive effects on liver tissue.

scholarly journals Comparison of Effects of High-Intensity Interval Training and Continuous Training on Memory and Correlation with Antioxidant Enzyme Activity in the Rat Brain

2021 ◽  
Vol 19 (2) ◽  
Author(s):  
Hossein Taheri Chadorneshin ◽  
Shila Nayebifar ◽  
Seyed-Hosein Abtahi-Eivary ◽  
Hossein Nakhaei
Keyword(s):  
High Intensity ◽  
Wistar Rats ◽  
Interval Training ◽  
Long Term Memory ◽  
High Intensity Interval Training ◽  
Continuous Training ◽  
Sod Activity ◽  
Term Memory ◽  
High Intensity Interval

Objectives: This study aimed to investigate the effects of six-week continuous training (CT) and high-intensity interval training (HIIT) protocols on brain superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) activities and their associations with short-, mid-, and long-term memory in albino Wistar rats. Methods: In this study, 18 male albino Wistar rats (5 months old) were randomly assigned to three equal (n = 6) groups of sedentary control (SC), CT, and HIIT. Both CT and HIIT regimens were performed for 6 consecutive days per week for 6 weeks. Results: Both HIIT and CT regimens increased short-, mid-, and long-term memory, and the alterations were greater following HIIT than CT. In addition, both HIIT and CT regimens significantly increased SOD activity, with a higher elevation following HIIT than CT. Moreover, brain SOD activity positively correlated with short-, mid-, and long-term memory. However, neither CT nor HIIT had a significant effect on brain GPX and CAT activities. Conclusions: The HIIT regimen is highly potential, as opposed to the CT regimen, to improve memory function through a greater increase in the SOD activity of the brain.

scholarly journals The effect of eight weeks of high intensity interval training and crocin consumption on the apoptotic genes expression in the liver tissue of male rats under chronic doxorubicin induction

2020 ◽  
pp. 323-335
Keyword(s):  
High Intensity ◽  
Liver Tissue ◽  
Training Session ◽  
Interval Training ◽  
Male Rats ◽  
Maximum Speed ◽  
High Intensity Interval Training ◽  
Male Wistar Rats ◽  
Irreversible Effects ◽  
High Intensity Interval

Background and Aim: The use of doxorubicin (Dox) in chemotherapy has irreversible effects on liver tissue. Therefore, the role of exercise activities and the use of antioxidants consumption on the mechanism of apoptosis induced by (Dox are not yet fully understood. Therefore, the aim of the present study was to investigate the effect of High Intensity Interval Training (HIIT) and crocin consumption on liver tissue apoptosis in male rats under chronic Dox induction. Materials and Methods: In this experimental study, 40 male Wistar rats (mean weight 200±20 g)and age range of 8 weeks divided into five groups including; 1: healthy control groups, 2: Dox (2 mg/kg in 7 doses), 3: Dox+crocin (10 mg/kg), 4: Dox+HIIT, and 5: Dox+HIIT+crocin. The training groups ran for 8 weeks, 5 days a week, at 2-minute intervals and with an intensity of 80 to 90% of the maximum speed. 48 hours after the last training session, liver biopsy were performed to assess the fibrosis and expression of Bax and Bcl-2 genes by (Real time-PCR) method. The results of statistical analysis were analyzed using by one-way (ANOVA), at p≤0.05. Results: Dox significantly increased Bax expression compared to Bax/Bcl-2 and also decreased Bcl-2 expression in liver tissue of the patient groups (p=0.001). In contrast, crocin and the combination of exercise and crocin decreased Bax expression compared to Bax/Bcl-2 and increased Bcl-2 expression in experimental groups compared to Dox group (p=0.001). Conclusion: It seems the high intensity interval training with consumption of crocin has significant effect on the decrease of apoptosis in liver tissue in male rats subjected to chronic doxorubicin injection.

High intensity interval training increases the expression of hippocampus BDNF gene and decreases the serum tnf-α in Diabetic Rat

2020 ◽  
Vol 42 (5) ◽  
pp. 591-600
Author(s):  
Shaghayegh Abbasi ◽  
Neda Khaledi ◽  
Hossein Askari
Keyword(s):  
Gene Expression ◽  
High Intensity ◽  
Wistar Rats ◽  
Interval Training ◽  
Functional Test ◽  
High Intensity Interval Training ◽  
Bdnf Gene ◽  
Tnf Α ◽  
Male Wistar Rats ◽  
High Intensity Interval

Background: Diabetics have many disabilities, including neuronal damege hippocampus, and BDNF is an effective factor in this field. This factor has the most impact on the hippocampus, also TNF-α is an inflammatory factor that increases in diabetes, the purpose of this study was to investigate the effect of 6 weeks of High Intensity interval training on the gene expression of brain-derived neurotrophic factor (BDNF) in the hippocampus of male Wistar rats. Methods: For this study, 48 male Wistar rats (4 weeks old) with 150± 10 g weight were categorized in 4 groups of: diabetic rats (n= 12), exercise diabetic (n= 12), control (n= 12) and exercise control (n= 12). For induction of diabetes, peritoneal injection of STZ solution (50 mg/kg) was used. After a week of familiarization with the environment and practice, high Intensity interval training protocol consisted of 3 days of training per week for 6 weeks with 50% to 110% of maximum oxygen consumption was performed. 24 hours after the completion of the exercise, the functional test was taken and the animals were autopsy 48 hours after the functional test. Finally, BDNF gene expression was evaluated using Real Time PCR technique. Results: Regarding the results, it was found that the high Intensity interval training intensified the expression of the BDNF gene and decreased the expression of the TNF-α protein. also The weight gain of the hippocampus has been observed along with increased expression of BDNF gene in the diabetic high Intensity interval training group. Conclusion: Exercise can help prevent hippocampus tissue loss, and also prevent memory damage that caused by diabetes. Also, high Intensity interval training are effective in maintaining the physical fitness of diabetics, therefore high Intensity interval training are recommended for improvement in the physical condition and life of diabetics.

scholarly journals OR-003 Time-sequential Changes of Myocardial AMPK and PGC-1α Expression during Detraining between High-intensity Interval Training and Moderate-intensity Continuous Training on Wistar Rats

2018 ◽  
Vol 1 (2) ◽  
Author(s):  
Guohuan Cao ◽  
Xinyi Chen ◽  
Hao Su
Keyword(s):  
High Intensity ◽  
Wistar Rats ◽  
Interval Training ◽  
Moderate Intensity ◽  
Specific Information ◽  
High Intensity Interval Training ◽  
Continuous Training ◽  
High Intensity Interval ◽  
Expression Characterization

Objective Without appropriate training stimulus, the athlete experiences a loss of the physiological adaptations brought about by exercise. In most of highly trained athletes, short of training induces a rapid decline in VO2max, but it remains above control values. However, there is no specific information for normal people about the effects of detraining during certain period aerobic training. Therefore, the purpose of this study was to examine the effects of detraining between high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on myocardial AMPK and PGC-1α expression characterization time-sequential changes in Wistar rats, as well as the changing characteristics between AMPK and PGC-1α expression characterization and cardiacrespiratory fitness (CRF). Moreover, the potential mechanism for exercise arrangement was also investigated. Methods In this study, 27 four-month-old male Wistar rats were randomly divided into the sedentary control group (C), MICT group (M) and HIIT group (H).Animals in the training groups ran on a treadmill 5 days/week during 6 weeks. HIIT group (70%-90%-50%VO2max) and MICT (50%VO2max) group was ran for 50min exercise every training day. All the rats free to gather the food and drinking water. All rats were measured the VO2max after a week adaptive training and then the M group and H group began to exercise intervention. After 6 weeks of training, rats were randomly selected from each group at the 24h, the 3rd day, the 7th day and the 10th day. The Maximal oxygen uptake test was carried out before the samples were taken, and the abdominal aortic blood, myocardium and other tissues were collected after anesthesia. The expression characterization of AMPK and PGC-1α was tested by Western blotting analysis. All statistical analyses were performed using SPSS 17.0 and GraphPad Prism 5.01 for Windows. Data was presented as mean and standard deviation（SD), unless otherwise stated. The two-way ANOVA (intervention × time) with repeated measures were used to analyze differences of HIIT and MICT with time-sequential. One-way ANOVA was used to compare the difference between time-sequential among the groups for each variable. The relationship between variables was assessed using the Pearson correlation coefficient. The expression characterization of the detraining effect was also assessed using Cohen’s d effect sizes (ES) and thresholds (<0.5=small; 0.5~0.79=moderate;≥0.8=large). The level of significance was set at P<0.05 and the confidence intervals at 95%. Results VO2max showed a gradual downward trend in both H and M groups throughout the 10 days detraining periods. Detraining in the 10th day, training cessation resulted in the VO2max of H and M group were significantly lower than detraining 24h. (P<0.05). Detraining in 3rd day, myocardial AMPK and PGC-1α increased in H group, it was significantly higher than the C group (P<0.05), but there is no differences in the other detraining days(P>0.05). Furthermore, detraining in the 7th day myocardial PGC-1α decreased in H group, this value was significantly lower than detraining 24h (P<0.05). Detraining in 7th day, myocardial AMPK and PGC-1α started decreasing, but it was not significant than C group or other detraining days (P>0.05). Conclusions (1)The present data suggest that 6 weeks HIIT and MICT can increase the expression of myocardial AMPK and PGC-1α, the VO2max training effects disappeared after 10 days detraining. (2)Detraining during the 3rd day and the 7th day was the critical time point for retraining, endurance training intervention should be arranging among these days. (3)The VO2max time-sequential changes was partially consistent with AMPK and PGC-1α expression characterization, but AMPK and PGC-1α expression characterization was more sensitive than VO2max.

High-intensity interval training improves interventricular synchrony and strain under hypoxic stress in sedentary males

2020 ◽  
Vol 41 (Supplement_2) ◽  
Author(s):  
Y.C Huang ◽  
J.S Wang
Keyword(s):  
High Intensity ◽  
Interval Training ◽  
Moderate Intensity ◽  
Control Group ◽  
Funding Source ◽  
Peak Strain ◽  
High Intensity Interval Training ◽  
The Difference ◽  
High Intensity Interval ◽  
Longitudinal Strains

Abstract Background Interventricular interactions in increased RV afterload such as hypoxia stress, which affects both synchrony and function in an in-series effect as well as a parallel effect arising from leftward septal shift. Improved myocardial contractility is a critical circulatory adaptation to exercise training, however, the types of exercise that can improve interventricular synchrony under hypoxic environment have not yet been established. Purpose This study investigates how high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) influence on the interventricular synchrony in response to normobaric hypoxia in sedentary men. Methods Fifty-four sedentary males were randomized to perform HIIT (3-minute intervals at 40% and 80% VO2peak, n=18), MICT (sustained 60% VO2peak, n=18) for 30 minutes/day, 5 days/week for 6 weeks and the control group (CTL, n=18). Synchrony measurements at apical 4-chamber view including (1) intra-delay, the difference in time to peak strain (TS) between segmental septal-to-lateral ventricular or atrial walls, and (2) inter-delay, the difference in TS between RV or RA free wall and LV or LA lateral wall. The data were acquired by 2-dimensional speckle tracking echocardiography at rest under hypoxic condition (12% FIO2, simulated an altitude of 4,500 m) before and after the interventions. Results HIIT had significantly elevated radial and longitudinal strains in both LA and LV (p&lt;0.05). As the results showed, HIIT was superior than MICT in improvement of longitudinal intra-delay of LV; furthermore, only HIIT simultaneously ameliorated both radial and longitudinal synchrony at apex. Although the HIIT enhanced intraventricular synchrony in both motions, whereas the interventricular synchrony deteriorated at radial motion. In atrium synchrony, although both groups augmented the intra-LA synchrony, however, only HIIT reduced the inter-delay between LA and RA at the roof motion. LV end-systolic volume (ESV) significantly correlated with the longitudinal inter-delay of ventricle (r=−0.53, p&lt;0.05), whereas the LV end-diastolic volume (EDV) correlated with the inter-delay of atrium in roof motion (r=0.40, p&lt;0.05). Conclusion We found differences between HIIT and MICT in segmental intra- and inter- synchrony. HIIT enhanced both ventricular or atrial synchrony, and further increased the EDV coupled with decreased ESV. These findings give new insight into cardiac adaptation to difference endurance training and the long-term impact of such changes warrants future study in cardiac diseases. Funding Acknowledgement Type of funding source: Public grant(s) – National budget only. Main funding source(s): National Science Council of Taiwan

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