scholarly journals Emergence of SCCmec Type I Obtained from Clinical Samples in Shiraz Teaching Hospitals, South-West of Iran

2015 ◽  
Vol 8 (5) ◽  
Author(s):  
Hadi Sedigh Ebrahim-Saraie ◽  
Mohammad Motamedifar ◽  
Jamal Sarvari ◽  
Seyedeh Mahsan Hoseini Alfatemi
Keyword(s):  
Sccmec Type ◽  
Teaching Hospitals ◽  
Clinical Samples ◽  
Type I ◽  
South West

The Characterization of mecA Gene and SCCmec Typing in Clinical Samples of MRSA

2019 ◽  
Vol 13 (2) ◽  
pp. 1
Author(s):  
Ali Jawad ◽  
Aziyah Abdul Aziz
Keyword(s):  
Methicillin Resistance ◽  
Sccmec Type ◽  
Meca Gene ◽  
Teaching Hospitals ◽  
Clinical Samples ◽  
Type I ◽  
Biochemical Tests ◽  
Type Iii ◽  
Sccmec Typing ◽  
Type Iv

MRSA is a major pathogen worldwide and its infections are associated with increased morbidity and mortality, in comparison with other S. aureus infections. The study was designed to characterize the mecA gene and staphylococcal cassette chromosome (SCCmec) in methicillin resistance S. aureus (MRSA). A total of 20 presumptive S. aureus strains were collected from one of the teaching hospitals in Selangor. Using standard biochemical tests, all the isolates were verified as S. aureus. When tested against cefoxitin, 80% of the isolates were confirmed as MRSA. All the MRSA isolates were further subjected to polymerase chain reaction (PCR) to detect the presence of mecA gene. Nine out of the 16 MRSA isolates (56%) were mecA positive, whereas the remaining four were mecA negative. The 16 MRSA with positive mecA gene were further subjected to SCCmec typing of type I, II, III, IV and V. The most frequent SCCmec types were type III (56%) followed by type II (33%), and type IV (11%). None of the isolates were of SCCmec type I or V. Our study indicates that SCCmec type III is predominant among the isolates which is in agreement with other studies conducted on clinical strains of MRSA.  

scholarly journals The SCCmec Types and Antimicrobial Resistance among Methicillin-Resistant Staphylococcus Species Isolated from Dogs with Superficial Pyoderma

2021 ◽  
Vol 8 (5) ◽  
pp. 85
Author(s):  
Yuttana Chanayat ◽  
Areerath Akatvipat ◽  
Jeff B. Bender ◽  
Veerasak Punyapornwithaya ◽  
Tongkorn Meeyam ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Sccmec Type ◽  
Small Animal ◽  
Control Measures ◽  
Infection Prevention And Control ◽  
Clinical Samples ◽  
Biochemical Tests ◽  
Methicillin Resistant ◽  
Type V ◽  
Staphylococcal Cassette Chromosome

This study characterizes clinical methicillin-resistant staphylococcal (MRS) isolates obtained from superficial pyoderma infections in dogs. Our interest was to determine the staphylococcal cassette chromosome mec (SCCmec) type and the antimicrobial susceptibility among MRS isolates from clinical cases. Skin swabs were collected and cultured. Staphylococcus species were identified and characterized with biochemical tests and MALDI-TOF-MS and antimicrobial susceptibility testing by disk diffusion. mecA detection and staphylococcal cassette chromosome mec (SCCmec) typing were achieved by PCR. Of the 65 clinical samples, 56 (86.2%) staphylococcal infections were identified. Twelve (21%) of 56 isolates were MRS infections. All MRS isolates were multidrug resistant. The ccrC and class-C2 mec, which were SCCmec type V, were the most prevalent (66.7%) among the 12 MRS isolates. The predominant SCCmec type V was found in S. aureus, S. intermedius group, S. lentus, S. xylosus, and S. arlettae. Treatment failure is a concern with the emergence of highly resistant MRS in dogs associated with superficial pyoderma. The detection of type V SCCmec MRS has previously been reported among veterinarians and dog owners but not in Northern Thailand. These infections serve as a reminder to improve infection prevention and control measures including reducing environmental contamination and potential zoonotic exposures to MRS. In addition, educational awareness of these risks in small animal hospitals needs to be increased among veterinary hospital staff, clients, and patients.

scholarly journals Virulence Factors Found in Nasal Colonization and Infection of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates and Their Ability to Form a Biofilm

Toxins ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 14
Author(s):  
Thamiris Santana Machado ◽  
Felipe Ramos Pinheiro ◽  
Lialyz Soares Pereira Andre ◽  
Renata Freire Alves Pereira ◽  
Reginaldo Fernandes Correa ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Sccmec Type ◽  
Protein A ◽  
Invasive Infection ◽  
Type I ◽  
Methicillin Resistant ◽  
Type Iv ◽  
Spa Gene ◽  
The City

Hospitalizations related to Methicillin-resistant Staphylococcus aureus (MRSA) are frequent, increasing mortality and health costs. In this way, this study aimed to compare the genotypic and phenotypic characteristics of MRSA isolates that colonize and infect patients seen at two hospitals in the city of Niterói—Rio de Janeiro, Brazil. A total of 147 samples collected between March 2013 and December 2015 were phenotyped and genotyped to identify the protein A (SPA) gene, the mec staphylococcal chromosomal cassette (SCCmec), mecA, Panton-Valentine Leucocidin (PVL), icaC, icaR, ACME, and hla virulence genes. The strength of biofilm formation has also been exploited. The prevalence of SCCmec type IV (77.1%) was observed in the colonization group; however, in the invasive infection group, SCCmec type II was prevalent (62.9%). The Multilocus Sequence Typing (MLST), ST5/ST30, and ST5/ST239 analyses were the most frequent clones in colonization, and invasive infection isolates, respectively. Among the isolates selected to assess the ability to form a biofilm, 51.06% were classified as strong biofilm builders. Surprisingly, we observed that isolates other than the Brazilian Epidemic Clone (BEC) have appeared in Brazilian hospitals. The virulence profile has changed among these isolates since the ACME type I and II genes were also identified in this collection.

scholarly journals The face of hypervirulent Klebsiella pneumoniae isolated from clinical samples of two Iranian teaching hospitals

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Rahimeh Sanikhani ◽  
Mohammad Moeinirad ◽  
Hamid Solgi ◽  
Azar Hadadi ◽  
Fereshteh Shahcheraghi ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Antimicrobial Resistance ◽  
Klebsiella Pneumoniae ◽  
High Resistance ◽  
Multidrug Resistant ◽  
Teaching Hospitals ◽  
Clinical Samples ◽  
Clonal Relatedness ◽  
The Face ◽  
Global Concern

AbstractHypervirulent Klebsiella pneumoniae (hvKp) has emerged as a pathogen of global concern. In this study, both phenotypic and genotypic tests were used to detect hvKp. Antimicrobial resistance profiles and clonal relatedness of clinical isolates were also determined. We found that 34.2% (163/477) of the isolates were tellurite resistant, and among them 102 hvKp isolates detected with iucA or iutA or peg-344 as molecular markers. The blaSHV (80.4%), followed by blaCTX-M-15 (76.5%) and blaTEM (67.6%), blaOXA-48 (53.9%), and blaNDM-1 (32.3%) were detected, while blaKPC-1 was not present in any hvKp isolates. It was found that the majority of hvKp isolates belonged to capsular serotype K20 and ompK36 group C, which is related to clonal group (CG) 23 (e.g. ST23). A high percentage of multidrug-resistant hvKp (76.6%) and high resistance to imipenem (67%) indicated a serious problem that should be addressed in the clinical setting.

scholarly journals The Face of Hypervirulent Klebsiella Pneumoniae (hvKp) Isolated from Clinical Samples of Two Iranian Teaching Hospitals

2021 ◽  
Author(s):  
Rahimeh Sanikhani ◽  
Mohammad Moeinirad ◽  
Hamid Solgi ◽  
Azar Haddadi ◽  
Fereshteh Shahcheraghi ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Klebsiella Pneumoniae ◽  
Multidrug Resistant ◽  
Teaching Hospitals ◽  
Clinical Samples ◽  
Clinical Settings ◽  
Urgent Problem ◽  
Clonal Relatedness ◽  
The Face ◽  
Global Concern

Abstract Hypervirulent Klebsiella pneumoniae (hvKp) has emerged as a pathogen of global concern. In this study, both phenotypic and genotypic tests were used to detect hvKp. Antimicrobial resistance profiles and clonal relatedness of clinical isolates were also determined. We found that 62.6% of the isolates were tellurite resistant and among them iucA or iutA or peg344 as hvKp molecular markers, were positive. The blaSHV (81.4%), followed by blaCTX−M15 (75.5%) and blaTEM (67.6%), blaOXA−48 (33.7%), blaNDM−1 (32.3%) were detected, while blaKPC−1 was not present in any hvKp isolates. It was found that the majority of hvKp isolates belonged to capsular serotype K20 and ompK36 group C, which is related to CG23 (e.g. ST23). A high percentage of multidrug-resistant hvKp (MDR-hvKp) and high resistance to imipenem (66%) indicated that there is an urgent problem that should be addressed in the clinical settings.

scholarly journals Profile.

2016 ◽  
Vol 4 (1) ◽  
pp. 5
Author(s):  
Bruna Gerardon Batista ◽  
Pedro Alves d' Azevedo
Keyword(s):  
Health Care ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Sccmec Type ◽  
Genetic Profile ◽  
Type I ◽  
Porto Alegre ◽  
Type Iii ◽  
Sccmec Types ◽  
Common Infections

Methicillin-resistant Staphylococcus aureus (MRSA) is anopportunistic pathogen that affectspublic health representing the most common infections related to health care and community. MRSA infections are classified as health care-associated MRSA (HA-MRSA) and community-associated MRSA (CA-MRSA) defined by the bacteria genetic profile. This study conducts a molecular characterization of eighty-one MRSA isolates from a hospital in Porto Alegre, RS, Brazil, in a period from January to June of 2012. A multiplex PCR was performed to determine the SCCmec types. From the 81 isolates, 24 (30%) were type I, 10 (12%) type II, 21 (26%) type III, 4 (5%) type IVa, 12 (15%) isolates were type IVc, 1 isolate was type I and IVc (1%) and 1 was type III and IVc (1%) simultaneously, while non-typable isolates corresponded to 8 isolates (10%). However most of the isolates were carrying SCCmec types related to HA-MRSA, the results reveal a change in the epidemiology, considering the decrease of the incidence of SCCmec type III and the increase of isolates being typed as SCCmec I.

PI3K Pathway in Gynecologic Malignancies

2013 ◽  
pp. e218-e221
Author(s):  
Helga B. Salvesen ◽  
Henrica Maria Werner ◽  
Camilla Krakstad
Keyword(s):  
Gynecologic Cancer ◽  
Predictive Biomarkers ◽  
Pi3k Pathway ◽  
Clinical Samples ◽  
Type I ◽  
Cancer Type ◽  
Gynecologic Malignancies ◽  
Ovarian Carcinomas ◽  
Pik3ca Mutations ◽  
Pathway Activation

Alterations in PI3K signaling are common in gynecologic malignancies. Alterations detected vary with gynecologic cancer type, histologic subtypes within these, and clinical phenotypes. The distinction into type I and type II endometrial and ovarian carcinomas is reflected in distribution of changes detected in several of the PI3K members. PIK3CA mutations and amplifications are common in endometrial, ovarian, and cervical cancers. PTEN mutations and deletions are frequent in endometrial cancers. Several immunohistochemical studies of protein expression have explored these and other potential surrogate markers for PI3K pathway activation. Biomarkers to measure level of PI3K activity in clinical samples are not established. Whether amplifications, mutations, and deletions of the PI3K pathway members, and in particular change in their expression levels, result in clinically relevant pathway activation needs to be further explored. Also, to what extent these alterations drive the tumor behavior and are critical targets for therapeutics to improve patient survival needs to be further tested to establish predictive biomarkers for response to PI3K inhibition.

scholarly journals The Face of Hypervirulent Klebsiella Pneumoniae (hvKp) Isolated from Clinical samples of Two Iranian Teaching Hospitals

2021 ◽  
Author(s):  
Rahimeh Sanikhani ◽  
Mohammad Moeinirad ◽  
Hamid Solgi ◽  
Azar Hadadi ◽  
Fereshteh Shahcheraghi ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Klebsiella Pneumoniae ◽  
Multidrug Resistant ◽  
Teaching Hospitals ◽  
Clinical Samples ◽  
Clinical Settings ◽  
Urgent Problem ◽  
Clonal Relatedness ◽  
The Face ◽  
Global Concern

Abstract Hypervirulent Klebsiella pneumoniae (hvKp) has emerged as a pathogen of global concern. In this study, both phenotypic and genotypic tests were used to detect hvKp. Antimicrobial resistance profiles and clonal relatedness of clinical isolates were also determined. We found that 62.6% of the isolates were tellurite resistant and among them iucA or iutA or peg344 as hvKp molecular markers, were positive. The blaSHV (81.4%), followed by blaCTX−M15 (75.5%) and blaTEM (67.6%), blaOXA−48 (33.7%), blaNDM−1 (32.3%) were detected, while blaKPC−1 was not present in any hvKp isolates. It was found that the majority of hvKp isolates belonged to capsular serotype K20 and ompK36 group C, which is related to CG23 (e.g. ST23). A high percentage of multidrug-resistant hvKp (MDR-hvKp) and high resistance to imipenem (66%) indicated that there is an urgent problem that should be addressed in the clinical settings.

scholarly journals PUVAsol therapy in the management of vitiligo: outcome in a dermatology clinic in South-West Nigeria

2019 ◽  
Vol 5 (2) ◽  
pp. 247
Author(s):  
Patrick O. Echekwube ◽  
Olayinka A. Olasode ◽  
Emmanuel O. Onayemi
Keyword(s):  
Low Cost ◽  
Treatment Options ◽  
Tertiary Hospital ◽  
Adult Patients ◽  
University Teaching ◽  
Teaching Hospitals ◽  
Extensive Disease ◽  
Dermatology Clinic ◽  
South West ◽  
University Teaching Hospitals

<p class="abstract"><strong>Background:</strong> Vitiligo is a depigmentary dermatosis which currently has no cure but there are different treatment options available to treat affected patients with varying results. The aim of the study was to ascertain the effect of PUVAsol therapy offered to adult patients with vitiligo at a tertiary hospital in Nigeria.</p><p class="abstract"><strong>Methods:</strong> All consecutive adult patients with vitiligo who presented at the Obafemi Awolowo University Teaching Hospitals complex, Ile-lfe and gave consent were recruited for the study which was for a period of 6 months. Patients with limited disease were treated with topical PUVAsol and those with extensive disease were treated with oral PUVAsol. The outcome of therapy for the patients was classified into three categories as follows: progressed (P), stable (S) and repigmented (R). The repigmented group was further sub-classified into fair (R1) and good (R2) representing ≤50% and &gt;50% repigmentation of areas affected respectively.<strong></strong></p><p class="abstract"><strong>Results:</strong> After 6 months of therapy, most of the patients (92%) had repigmentation of their lesions while the remaining had either stable lesions (6%) or progression of their lesions (2%). Amongst the patients whose lesions got repigmented, 96% of them had repigmentation in less than half of the areas affected by vitiligo which was a fair outcome.</p><p class="abstract"><strong>Conclusions:</strong> PUVAsol is a treatment modality for vitiligo with some repigmentation of lesions following therapy for 6 months. PUVAsol therapy could be recommended in resource poor settings because of its low cost and availability in most parts of Nigeria.</p>

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