Mucin Stain on Frozen Sections

1999 ◽  
Vol 123 (5) ◽  
pp. 378-380
Author(s):  
Suchetha Soans ◽  
Lorenzo M. Galindo ◽  
Fernando U. Garcia
Keyword(s):  
Colonic Mucosa ◽  
Frozen Section ◽  
Staining Technique ◽  
Turnaround Time ◽  
Staining Procedure ◽  
Normal Colonic Mucosa ◽  
Frozen Sections ◽  
Tissue Sections ◽  
Paget Disease ◽  
Frozen Tissue

Abstract Context.—The importance of frozen-section diagnoses in the practice of pathology cannot be overemphasized. In some cases, the use of a mucin stain can greatly aid in the diagnosis. Since few methods for mucin staining have been described that can be used in the frozen-section setting, we developed one such staining procedure for mucin. Objective.—To develop a rapid mucicarmine staining technique to be used on frozen sections that does not significantly delay overall turnaround time. Design.—A standard mucicarmine staining technique was modified by using a concentrated mucicarmine stain and a microwave oven, to reduce the total staining time to 3 minutes or less. Frozen tissue from normal colonic mucosa was used as a control, and skin from extramammary Paget disease for evaluation of margins was used as a case. Results.—The rapid mucicarmine stain successfully demonstrated the presence of mucin on frozen-tissue sections. Mucin stained deep rose, and the connective tissue stained green. Conclusion.—This rapid and simple mucin staining technique can be used on frozen sections with no significant effect on the overall turnaround time, thereby aiding rapid diagnosis on frozen sections.

scholarly journals Rapid brain structure and tumour margin detection on whole frozen tissue sections by fast multiphotometric mid-infrared scanning

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tim Kümmel ◽  
Björn van Marwick ◽  
Miriam Rittel ◽  
Carina Ramallo Guevara ◽  
Felix Wühler ◽  
...  
Keyword(s):  
Imaging System ◽  
Frozen Section ◽  
Computational Effort ◽  
Primary Hepatocellular Carcinoma ◽  
Measuring System ◽  
Sufficient Information ◽  
Tissue Samples ◽  
Mid Infrared ◽  
Tissue Sections ◽  
Frozen Tissue

AbstractFrozen section analysis is a frequently used method for examination of tissue samples, especially for tumour detection. In the majority of cases, the aim is to identify characteristic tissue morphologies or tumour margins. Depending on the type of tissue, a high number of misdiagnoses are associated with this process. In this work, a fast spectroscopic measurement device and workflow was developed that significantly improves the speed of whole frozen tissue section analyses and provides sufficient information to visualize tissue structures and tumour margins, dependent on their lipid and protein molecular vibrations. That optical and non-destructive method is based on selected wavenumbers in the mid-infrared (MIR) range. We present a measuring system that substantially outperforms a commercially available Fourier Transform Infrared (FT-IR) Imaging system, since it enables acquisition of reduced spectral information at a scan field of 1 cm2 in 3 s, with a spatial resolution of 20 µm. This allows fast visualization of segmented structure areas with little computational effort. For the first time, this multiphotometric MIR system is applied to biomedical tissue sections. We are referencing our novel MIR scanner on cryopreserved murine sagittal and coronal brain sections, especially focusing on the hippocampus, and show its usability for rapid identification of primary hepatocellular carcinoma (HCC) in mouse liver.

Versatility of biotin-labeled lectins and avidin-biotin-peroxidase complex for localization of carbohydrate in tissue sections.

1982 ◽  
Vol 30 (2) ◽  
pp. 157-161 ◽  
Author(s):  
S M Hsu ◽  
L Raine
Keyword(s):  
Colonic Mucosa ◽  
Simple Technique ◽  
Medullary Carcinoma ◽  
Normal Breast ◽  
Normal Colonic Mucosa ◽  
Colonic Adenocarcinoma ◽  
Carcinoma Of The Breast ◽  
Cytoplasmic Staining ◽  
Tissue Sections ◽  
Membranous Staining

Carbohydrate residues in tissue can be localized by a very sensitive, specific, and simple technique, utilizing a biotin-labeled lectin followed by an avidin-biotin-peroxidase complex (ABC). Distribution of peanut receptors in benign and malignant tissues was found to be quite different. Normal colonic mucosa occasionally shows small dot staining in the supranuclear cytoplasm, while the tumor cells of colonic adenocarcinoma tend to show diffuse cytoplasmic or membranous staining. Normal breast ductal epithelium shows membranous staining at luminal borders, while medullary carcinoma of the breast shows distinct membranous and cytoplasmic staining. The use of this avidin-biotin-peroxidase complex technique may contribute to understanding the process of tumorigenesis as well as providing a sensitive method to determine early stages of malignant transformation.

scholarly journals Mixed glycosidase pretreatment reduces nonspecific binding of antibodies to frozen tissue sections.

1985 ◽  
Vol 33 (7) ◽  
pp. 695-698 ◽  
Author(s):  
L P Andrews ◽  
R K Clark ◽  
I Damjanov
Keyword(s):  
Monoclonal Antibody ◽  
Renal Tissue ◽  
Frozen Sections ◽  
Tissue Sections ◽  
Mouse Tissues ◽  
Background Staining ◽  
Frozen Tissue ◽  
Nonspecific Staining ◽  
Immunohistochemical Studies ◽  
Monoclonal Antibody Binding

Indirect immunohistochemical studies of frozen mouse tissues with mouse monoclonal antibodies yield, in general, suboptimal results primarily because of indiscriminate binding of secondary antibody to all mouse immunoglobulins, i.e., to the monoclonal reagent and to endogenous immunoglobulin nonspecifically trapped in the tissue. To reduce this nonspecific staining, frozen sections of mouse kidney were treated enzymatically. Optimal results were obtained following a 2 hr treatment with 20 mg/ml of mixed glycosidases (MG). This treatment reduced the nonspecific background staining of the interstitial spaces and blood vessels, but did not affect the reactivity of structurally bound immunoglobulin G (IgG) in the glomeruli or alter the reactivity of mouse renal tissue to the monoclonal antibody that recognizes an oligosaccharide antigenic determinant (SSEA-1). Eluates from enzyme-treated frozen tissue sections contained normally immunoreactive IgG in the form of dimers. These data indicate that MG treatment of frozen sections could be safely used to reduce the content of nonstructurally bound immunoglobulins in frozen tissues and thus improve the visualization of specific monoclonal antibody binding.

Measurement of the AST to LD Ratio in Parathyroid Tissue Suspension Can Precisely Differentiate a Hyperfunctioning Parathyroid

2020 ◽  
Vol 105 (8) ◽  
pp. e2764-e2769
Author(s):  
Toyone Kikumori ◽  
Takahiro Ichikawa ◽  
Takahiro Inaishi ◽  
Noriyuki Miyajima ◽  
Masahiro Shibata ◽  
...  
Keyword(s):  
Primary Hyperparathyroidism ◽  
Operating Characteristic ◽  
Frozen Section ◽  
Characteristic Curve ◽  
Treatment Success ◽  
Parathyroid Tissue ◽  
Turnaround Time ◽  
Frozen Sections ◽  
Intraoperative Parathyroid Hormone ◽  
Excised Tissue

Abstract Background Frozen section of excised tissue is used to confirm removal of the etiology of primary hyperparathyroidism in the current era of intraoperative parathyroid hormone measurement and provides safeguards for surgeons. We recently reported that the aspartate aminotransferase (AST)/lactate dehydrogenase (LD) ratio in tissue suspension can accurately distinguish normal parathyroid tissue from other tissues. Therefore, we hypothesized that this ratio may also be applied to distinguish hyperfunctioning parathyroid tissue (HPT) from other tissues. Methods We prospectively analyzed 22 patients who underwent parathyroidectomy for primary hyperparathyroidism (benign, 21; malignant, 1) from July 2018 to October 2019. In total, 27 specimens were examined. Approximately 1 mm3 of minced HPT as confirmed by frozen sections was suspended in 1 mL of normal saline and AST and LD levels were measured. The AST/LD ratios of other tissues (normal parathyroid tissue, thyroid gland, adipose tissue, and others; n = 94) were obtained from our previous report. Results The AST/LD ratio of benign HPT was consistently higher than that of other tissues (P < 0.001). The optimal cut-off value was 0.36 according to the receiver operating characteristic curve, with 100% sensitivity and specificity. The AST/LD ratio in malignant HPT was also markedly lower than that in benign HPT. Conclusion This method might be a new adjunct for intraoperative differentiation of HPT with an accuracy and turnaround time comparable with those of frozen sections, minimal cost, and no need for dedicated pathological staff. Additionally, this method might increase the treatment success rate in settings with limited medical resources.

Study of vitrified, unstained frozen tissue sections by cryoimmunoelectron microscopy

1991 ◽  
Vol 100 (1) ◽  
pp. 227-236
Author(s):  
I. Sabanay ◽  
T. Arad ◽  
S. Weiner ◽  
B. Geiger
Keyword(s):  
Heavy Metal ◽  
High Resolution ◽  
High Contrast ◽  
Frozen Sections ◽  
Microscope Examination ◽  
Tissue Sections ◽  
Novel Approach ◽  
Cytoplasmic Filaments ◽  
Frozen Tissue ◽  
Ultrathin Frozen Sections

We describe the development and application of a novel approach to high-resolution ultrastructural analysis of cells and tissues. It is based on the preparation of ultrathin frozen sections of fixed tissues, rinsing of the sections, followed by their embedding on the grid in a layer of vitrified ice, and direct observation with a cryoelectron microscope. Examination of smooth muscle, kidney and heart tissues showed that although no heavy metal staining was used, high-contrast images are obtained. Fine details of cytoplasmic filaments and organelles, membranes and membrane-associated structures, as well as connective-tissue elements are all visible. The new method is suitable for immunolabeling, including high resolution localization of specific molecules within the cytoplasm.

scholarly journals Role of Intraoperative Frozen Section Diagnosis in the Management of Patients with CNS Lesions: Evaluation of 60 Consecutive Intraoperative Frozen Sections from 1991 to 1993

2020 ◽  
Vol 1 (1) ◽  
Author(s):  
Seyed Mirsattari ◽  
Fraser W. Saunders
Keyword(s):  
Frozen Section ◽  
Imaging Techniques ◽  
Frozen Sections ◽  
Tissue Samples ◽  
Tissue Sections ◽  
Intraoperative Management ◽  
Clinical Diagnoses ◽  
Cns Lesions

To determine the role of intraoperative frozen sections (FSs) in the management of patients with central nervous system (CNS) lesions, 60 consecutive intraoperative clinical diagnoses of CNS lesions were presented and compared with concomitantly obtained FS diagnoses. Clinical diagnoses were established byhistory, physical examination, imaging techniques, and gross appearance of the abnormal tissue in situ. Tissue samples were obtained intraoperatively and processed for FS diagnoses. The findings of the FS diagnoses were reported to the operating room and compared with the clinical diagnoses. The remainingbiopsy samples were used to prepare paraffin-embedded tissue sections from which the definitive diagnoses were made. Comparison of the clinical and FS diagnoses, using paraffin-embedded tissue as the true diagnosis, shows that FS diagnosis has a limited contribution to intraoperative patient management by the neurosurgeon. The rate of diagnostic failures between the two techniques was very similar; clinical diagnoses and FSs were misinterpreted in 12 and 11 of the 60 cases, respectively. Compared to a clinical diagnosis, the intraoperative FS technique provided no significant improvement in diagnosis and management; it altered the intraoperative management of the patients in 2 of 60 cases.

Polymorphic Epithelial Mucin (PEM) Epitopes in the Estimation of the Sequence of Neoplastic Changes in Colon Adenocarcinoma

1993 ◽  
Vol 79 (1) ◽  
pp. 66-70
Author(s):  
Barbara Slesak ◽  
Antonina Harlozinska ◽  
Jadwiga Lapinska ◽  
Zbigniew Knapik
Keyword(s):  
Monoclonal Antibodies ◽  
Colonic Mucosa ◽  
Malignant Lesion ◽  
Colon Adenocarcinoma ◽  
Clear Correlation ◽  
Normal Colonic Mucosa ◽  
Tissue Sections ◽  
Adjacent Mucosa ◽  
Malignant Lesions ◽  
Cellular Abnormalities

Aims The relation between the expression of PEM epitopes and the sequence of neoplastic changes in individual patients with colon adenocarcinoma was investigated. Methode Immunohistochemical staining was performed using four monoclonal antibodies to PEM epitopes (139H2, 140C1, 115D8, 115F5) on tissue sections from frankly malignant lesions, adjacent mucosa, colonic mucosa taken 1 cm and 5 cm from the lesion and normal control mucosa. Results A clear correlation between PEM expression and the site of mucosa sampling was demonstrated. The expression of PEM molecules defined by all studied monoclonal antibodies was highest in colon adenocarcinoma, similar but slightly weaker in adjacent mucosa and colon mucosa taken 1 cm from the frankly malignant lesion, lowest in mucosa 5 cm from the lesion, and virtually absent in control normal colonic mucosa. Conclusions The immunological changes could be one of the first signs of a premalignant process and the estimation of PEM epitopes could show some cellular abnormalities not detectable by standard pathomorphological criteria.

scholarly journals Twenty Challenging Minutes to Take the Right Decision

2019 ◽  
Vol 4 (03) ◽  
Author(s):  
Dr. Priyanka P Shetty ◽  
Dr. Ramadas Naik ◽  
Dr Vineeth G. Nair ◽  
Dr Shubha Sudhakar ◽  
Dr Ali Abdul Latheef
Keyword(s):  
Diagnostic Accuracy ◽  
Predictive Value ◽  
Frozen Section ◽  
Turnaround Time ◽  
Frozen Sections ◽  
Formalin Fixed Paraffin ◽  
Frozen Section Diagnosis ◽  
Formalin Fixed Paraffin Embedded ◽  
The Right ◽  
Sensitivity Specificity

Background: The diagnostic accuracy of frozen section is an important source of information and a high risk procedure in surgical pathology. This is an important tool, not only in the management of surgical patients but also has a pivotal role in institution’s quality assurance in histopathology. Aims and objectives: To analyze indications, efficacy, accuracy, limitations, deferrals and turnaround time for all frozen sections in correlation with histopathology. Materials and methods: A retrospective analysis of all the frozen samples was done in correlation with permanent sections from June 2016 till July 2017. The diagnostic accuracy, sensitivity, specificity, turnaround time, deferrals and limitations were analysed. Results: A total of 45 specimens for frozen sections were sent to the department of Pathology during the time period. Out of these, 97.8% (n=44) were accepted and 2.2% (n=1) were deferred. Of the remaining 44 specimens, 1 (2.3%) was discordant and 43 (97.7%) were concordant to permanent diagnoses. Specimens were primarily from the ovary, breast and lymph nodes. The overall sensitivity, specificity, positive predictive value and negative predictive value of the frozen section compared to formalin fixed paraffin embedded sections (as gold standard) were 97.7%, 100%, 100% and 100% respectively. The average turn-around time for frozen section diagnosis was 23 minutes. Conclusion:  Frozen section diagnosis is an efficient, rapid, reliable and highly accurate tool in surgical diagnosis and management. The multidisciplinary approach helped avoiding limitations ensuring optimal patient care.

RESEARCH ON VALUE OF INTRAOPERATIVE FROZEN SECTION EXAMINATION OF THE COMMON TUMORS AT HUE UNIVERSITY HOSPITAL

2011 ◽  
pp. 67-73
Author(s):  
Cong Thuan Dang ◽  
Thi Thu Thao Le
Keyword(s):  
Sensitivity And Specificity ◽  
Frozen Section ◽  
Analysis Data ◽  
Poor Quality ◽  
University Hospital ◽  
Frozen Sections ◽  
Intraoperative Frozen Section ◽  
Frozen Section Examination ◽  
Incorrect Diagnosis ◽  
The Common

Background: To evaluate the accuracy and the pitfalls of frozen section examination in diagnosis the common tumors at Hue University Hospital. Materials and method: A retrospective analysis data of 99 consecutive patients from 2007 to 2009 were evaluated and analyzed the major pitfalls. In our 99 patients, 100% cases we compared histological diagnosis on frozen sections with those on paraffin sections. Results: The majority of frozen section examinations were the thyroid lesions 37.4%, breast lesions 25.2%, lymph nodes 16.1%, ovary 9.1% and less common in other diseases (12.1%). The accuracy, sensitivity and specificity of the intraoperative frozen section examination were 93.9%, 89.1% and 98.1% respectively. The main factors causing incorrect diagnosis in frozen section are: Misinterpretation, poor quality of frozen sections, improper sampling in sectioning and difficult to result interpretation. Conclusion: The frozen section analysis of suspect lesions displays good sensitivity and specificity characteristics.

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