Metabolomic profiling of crayfish haemolymph distinguishes sister species and sex: implications for conservation, aquaculture and physiological studies

2020 ◽  
Vol 25 (1) ◽  
pp. 89-101
Author(s):  
Emily D. Lette ◽  
Nathan G. Lawler ◽  
Quinton F. Burnham ◽  
Mary C. Boyce ◽  
Rodney Duffy ◽  
...  
Keyword(s):  
Captive Breeding ◽  
Biological Significance ◽  
Freshwater Crayfish ◽  
Liquid Chromatography Mass Spectrometry ◽  
Captive Breeding Program ◽  
Metabolomic Profiling ◽  
Potential Applications ◽  
Males And Females ◽  
Univariate Analyses ◽  
Metabolomic Approach

Abstract Hairy marron (Cherax tenuimanus Smith) are critically endangered freshwater crayfish found only in a single river in south-west Australia. Conservation efforts have included a captive breeding program, which has been largely unsuccessful, despite the closely related smooth marron (Cherax cainii Austin) being successfully bred for aquaculture. Using an untargeted liquid chromatography-mass spectrometry (LC-MS) metabolomic approach we created a profile of the metabolites in the haemolymph for males and females of the two species of marron. A non-lethal method was used to collect haemolymph and 84 reproducible annotated metabolites were identified. Variation in the levels of some metabolites were detected between species and between sexes within species. Multivariate analyses clearly differentiated the congeneric species and univariate analyses identified differences between species, sex and for some metabolite interactions between species and sex. This study created a baseline metabolome dataset for the two species and began to investigate the biological significance of metabolites that varied between species. We have shown metabolomics could be used for targeted studies to potentially assist reproductive success. This approach will be beneficial for conservation and aquaculture practices with potential applications for other aquatic taxa worldwide.

Analysis of gene origin in the first adult returns to the Cultus sockeye salmon captive breeding program

2011 ◽  
Vol 12 (6) ◽  
pp. 1469-1483 ◽  
Author(s):  
Ruth E. Withler ◽  
Robert H. Devlin ◽  
Steve Latham ◽  
Chris C. Wood ◽  
K. Janine Supernault
Keyword(s):  
Sockeye Salmon ◽  
Captive Breeding ◽  
Breeding Program ◽  
Captive Breeding Program ◽  
Gene Origin

scholarly journals Metabolomic profiling in patients with metabolic syndrome

Kardiologiia ◽  
2020 ◽  
Vol 60 (3) ◽  
pp. 37-43
Author(s):  
E. O. Korobkova ◽  
M. V. Kozhevnikova ◽  
I. S. Ilgisonis ◽  
G. A. Shakaryants ◽  
S. A. Appolonova ◽  
...  
Keyword(s):  
Risk Factors ◽  
Mass Spectrometry ◽  
Metabolic Syndrome ◽  
Amino Acids ◽  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Liquid Chromatography Mass Spectrometry ◽  
Metabolomic Profile ◽  
Additional Risk ◽  
Metabolomic Profiling

Objective. To identify biomarkers, which are most specific for patients with metabolic syndrome (MS) using metabolomic profiling.Materials and Methods. Metabolomic profiling of patients with MS and comparison of their profile with the profile of volunteers was performed using high-performance liquid chromatography-mass-spectrometry.Results. The metabolomic profile of MS patients differed in several amino acids, including choline, cysteine, and serine and in the acylcarnitine group (р<0.05 for all comparisons).Conclusion. The metabolites most specific for MS patients were identified. Increased concentrations of a combination of amino acids and carnitines can be considered as possible additional risk factors for cardiovascular diseases.

scholarly journals First Captive Breeding Program for the Endangered Pyrenean Sculpin (Cottus hispaniolensis Bacescu-Master, 1964)

Water ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 2986
Author(s):  
Joan Manubens ◽  
Oriol Comas ◽  
Núria Valls ◽  
Lluís Benejam
Keyword(s):  
Captive Breeding ◽  
Ex Situ Conservation ◽  
Breeding Program ◽  
Ex Situ ◽  
Major Influence ◽  
Freshwater Species ◽  
Non Profit ◽  
Captive Breeding Program ◽  
Critical Phases ◽  
Conservation Plans

The strong decline of freshwater fish species in Europe implies that further ex-situ conservation plans should be implemented in the near future. The present study reflects our experience with the Pyrenean sculpin (Cottus hispaniolensis Bacescu-Mester, 1964)—a small cottid endemic to the Hispano-French Garona River basin. In recent years, the Spanish Pyrenean sculpin population has reached a limit situation. Because of that, the non-profit association ADEFFA—with support from the public administration—started the first captive breeding program for this species in 2006. Fourteen years later, this study presents the results and evaluates the different steps of the program, with the aim of discussing and improving the ex-situ conservation plans for this and other cold freshwater species. There is a description and a comparison between six consecutive phases during the captive breeding process: nesting behaviour, courtship, egg fixation, parental care (incubation), hatching and survival during juvenile development. The purposes of this project are to: (1) identify the most determining phases for a successful captive breeding; (2) identify the factors that had a major influence to the success of the critical phases; and (3) increase the number of the offspring. This study is based on thirty-three wild individuals collected from Garona River (Val d’Aran, Spanish Pyrenees). During the program, twelve couples spawned in captive conditions, with around 2300 eggs laid. Eight couples bred successfully, with 751 hatched individuals and 608 juveniles reared. The analysis of each step of the captive breeding does not reveal significant differences between phases, so it can be concluded that they are all critical at the same level. In the literature, similar study-cases of captive breeding programs identify incubation and survival phases as the most critical. Consequently, the management made for this project has probably allowed to overcome in part the main impediments described in other similar programs.

scholarly journals DMSO Reductase Family: Phylogenetics and Applications of Extremophiles

2019 ◽  
Vol 20 (13) ◽  
pp. 3349 ◽  
Author(s):  
Jose María Miralles-Robledillo ◽  
Javier Torregrosa-Crespo ◽  
Rosa María Martínez-Espinosa ◽  
Carmen Pire
Keyword(s):  
Redox Reactions ◽  
Molecular Mechanisms ◽  
Biological Significance ◽  
Anaerobic Respiration ◽  
Biogeochemical Cycles ◽  
Free Software ◽  
Transfer Processes ◽  
Expression Of Genes ◽  
Potential Applications ◽  
Domains Of Life

Dimethyl sulfoxide reductases (DMSO) are molybdoenzymes widespread in all domains of life. They catalyse not only redox reactions, but also hydroxylation/hydration and oxygen transfer processes. Although literature on DMSO is abundant, the biological significance of these enzymes in anaerobic respiration and the molecular mechanisms beyond the expression of genes coding for them are still scarce. In this review, a deep revision of the literature reported on DMSO as well as the use of bioinformatics tools and free software has been developed in order to highlight the relevance of DMSO reductases on anaerobic processes connected to different biogeochemical cycles. Special emphasis has been addressed to DMSO from extremophilic organisms and their role in nitrogen cycle. Besides, an updated overview of phylogeny of DMSOs as well as potential applications of some DMSO reductases on bioremediation approaches are also described.

Differences in skeletal characters between the disjunct eastern and western populations of ring-billed gulls (Larus delawarensis)

1984 ◽  
Vol 62 (6) ◽  
pp. 1067-1074 ◽  
Author(s):  
Beatrice M. Termaat ◽  
John P. Ryder
Keyword(s):  
Discriminant Function ◽  
Multivariate Analyses ◽  
Correct Identification ◽  
Adult Males ◽  
Lake Of The Woods ◽  
Larus Delawarensis ◽  
Males And Females ◽  
Two Populations ◽  
Univariate Analyses ◽  
Discriminant Function Analyses

Univariate analyses of variance, multivariate analyses of variance (Manova), and discriminant function analyses were performed on measurements of 51 skeletal characters of adult males and females from the disjunct eastern and western populations of the ring-billed gull (Larus delawarensis) in Canada. The analyses established sufficient differences in skeletal characters to permit correct identification of the origin of 34 of 38 males (90%) and 40 of 42 females (95%). The populations are now expanding in range to the extent where new colonies are established in the Lake of the Woods, Ontario region, the historical hiatus between the two populations. Further expansion and contact may well erase the existing skeletal differences of individuals in these populations.

Risk Assessment of Inbreeding and Outbreeding Depression in a Captive-Breeding Program

2014 ◽  
Vol 28 (2) ◽  
pp. 529-540 ◽  
Author(s):  
NJAL ROLLINSON ◽  
DAVE M. KEITH ◽  
AIMEE LEE S. HOUDE ◽  
PAUL V. DEBES ◽  
MEGHAN C. MCBRIDE ◽  
...  
Keyword(s):  
Risk Assessment ◽  
Captive Breeding ◽  
Breeding Program ◽  
Outbreeding Depression ◽  
Captive Breeding Program
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