Antifungal and antibacterial activity of the different parts of mature Benincasa hispida against various microbial infectious agents

The emergence of multidrug resistant bacterial pathogens and the increase of antimicrobial resistance constitutes a major health challenge, leading to intense research efforts being focused on the discovery of novel antimicrobial compounds. In this study, endophytes were isolated from different parts of Sandwithia guyanensis plant (leaves, wood and latex) belonging to the Euphorbiaceae family and known to produce antimicrobial compounds, and chemically characterised using Molecular Network in order to discover novel antimicrobial molecules. One fungal endophyte extract obtained from S. guyanensis latex showed significant antimicrobial activity with Minimal Inhibitory Concentration on methicillin-resistant Staphylococcus aureus at 16 µg/mL. The chemical investigation of this fungus (Lecanicillium genus) extract led to the isolation of 5 stephensiolides compounds, four of which demonstrated antibacterial activity. Stephensiolide I and G showed the highest antibacterial activity on MRSA with a MIC at 4 and 16 µg/mL respectively.

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Study of the effect of the different parts of Morinda citrifolia L. (noni) on the green synthesis of silver nanoparticles and their antibacterial activity

Applied Surface Science ◽

10.1016/j.apsusc.2020.147855 ◽

2021 ◽

Vol 537 ◽

pp. 147855

Author(s):

Violeta Morales-Lozoya ◽

Heriberto Espinoza-Gómez ◽

Lucía Z. Flores-López ◽

Erika Lis Sotelo-Barrera ◽

Alfredo Núñez-Rivera ◽

...

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Activity ◽

Green Synthesis ◽

Morinda Citrifolia ◽

Different Parts

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ANTIBACTERIAL ACTIVITY OF ISOLATED ENDOPHYTIC FUNGI FROM RAUVOLFIA SERPENTINA (L.) BENTH. EX KURZ

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2016v8i11.9733 ◽

2016 ◽

Vol 8 (11) ◽

pp. 38 ◽

Cited By ~ 3

Author(s):

Rajshree Sahu ◽

Suneel Kumar ◽

Ravindra Prasad Aharwal ◽

Sardul Singh Sandhu

Keyword(s):

Escherichia Coli ◽

Antibacterial Activity ◽

Bacillus Subtilis ◽

Klebsiella Pneumoniae ◽

Endophytic Fungi ◽

Fungal Strain ◽

Penicillium Citrinum ◽

Rauvolfia Serpentina ◽

Molecular Sequencing ◽

Different Parts

Objective: The aim of the present study was to isolate the endophytic fungi from medicinal plant Rauvolfia serpentina (L.) Benth. ex Kurz. (Family Apocynaceae) and observed their antibacterial activity against bacteria as well as the molecular characterization of most potent fungal strain. Methods: Collection and isolation of endophytic fungi from different parts (root, shoot, leaves) of Rauvolfia serpentina plant. Screening of endophytic fungi for antibacterial activity was scrutinised against six bacteria viz. Bacillus subtilis, Enterococcus sp., Klebsiella pneumoniae, Escherichia coli, Salmonella typhimurium and Streptococcus pyogenes by using Agar well diffusion method. For molecular sequencing of potent fungi, the DNA was extracted, quantified and amplified by using two oligonucleotide primers ITS4 and ITS6 in PCR.Results: A total seven endophytic fungi Aspergillus niger, Penicillium citrinum, Cladosporium sp., Curvularia lunata, Aspergillus sp., Alternaria sp. and Aspergillus fumigatus were isolated from different parts of Rauvolfia serpentina and fungal strain Penicillium citrinum was shown the maximum zone of inhibition against Bacillus subtilis (23.0±0.12 mm), Escherichia coli (19.9±0.16 mm), Streptococcus pyrogens (19.2±0.59 mm), Enterococcus sp., (17.2±0.08 mm), Klebsiella pneumoniae (18.9±0.16 mm) and Salmonella typhimurium (15.1±0.16 mm). The molecular sequencing of the potent fungi was done by primers (ITS4 and ITS6) which showed strong specificity with fungal DNA and the percentages of identical matches of ITS4 and ITS6 DNA sequences in the GeneBank database (NCBI) were determined to 98 %.Conclusion: In the present study, the endophytic fungal strain Penicillium citrinumshowed the potential source of antibacterial bioactive compounds and molecular sequencing of this fungus helps in future to determine the various metabolic pathways that are responsible for the production of such type of novel compounds.

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Antioxidant and antibacterial activity of different parts of Leucas aspera

Asian Pacific Journal of Tropical Biomedicine ◽

10.1016/s2221-1691(12)60037-9 ◽

2012 ◽

Vol 2 (3) ◽

pp. 176-180 ◽

Cited By ~ 44

Author(s):

Ai Lan Chew ◽

Jeyanthi James Antony Jessica ◽

Sreenivasan Sasidharan

Keyword(s):

Antibacterial Activity ◽

Leucas Aspera ◽

Different Parts

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Essential oil composition and antibacterial activity of the different parts ofThymus maroccanusBall: an endemic species in Morocco

Natural Product Research ◽

10.1080/14786419.2013.768989 ◽

2013 ◽

Vol 27 (18) ◽

pp. 1700-1704 ◽

Cited By ~ 6

Author(s):

R. Belaqziz ◽

F. Bahri ◽

A. Romane ◽

S. Antoniotti ◽

X. Fernandez ◽

...

Keyword(s):

Antibacterial Activity ◽

Essential Oil ◽

Endemic Species ◽

Essential Oil Composition ◽

Oil Composition ◽

Different Parts

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Phytochemical, Antioxidant and Antimicrobial studies of Lannea egregia Engl. & K. Krause (Anacardiaceae) extracts and chromatographic fractions

Journal of Phytomedicine and Therapeutics ◽

10.4314/jopat.v19i1.4 ◽

2020 ◽

Vol 19 (1) ◽

pp. 348-363

Author(s):

Philip A. Idowu ◽

Loveth C. Ekemezie ◽

Charles O. Olaiya

Keyword(s):

Antibacterial Activity ◽

Ethyl Acetate ◽

Antioxidant Capacity ◽

Reducing Power ◽

Stem Bark ◽

Diffusion Method ◽

Total Phenolic ◽

Phytochemical Screening ◽

Crude Extracts ◽

Different Parts

Screening ‘new’ medicinal plants of traditional importance for bioactive components is a sure way of discovering novel therapeutic agents to treat diseases. This study, therefore investigated the presence of phytochemical, antioxidant and antibacterial components of the extracts of Lannea egregia. Phytochemical screening was done by standard methods. Antibacterial activity of the extracts of Lannea egregia was determined by agar well diffusion method while the minimum inhibitory concentration (MIC) was determined by agar dilution method. The antioxidant capacity of the crude extracts was determined through the evaluation of total flavonoid content, total phenolic content, ferric reducing power, total antioxidant capacity and 2,2-diphenyl-1-picryhydrazyl. The phytochemical screening of the different parts of this plant revealed the presence of tannins, saponins, alkaloids, flavonoids, steroids, terpenoids, emodins, phlobatannins, anthocyanins, coumarins and phenolics. Phlobatannins was observed to be absent in the stem bark. The crude extracts obtained from the leaves, stem bark and roots of this plant exhibited good antibacterial activity against typed strains of Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa and Escherichia coli. The diameter of the zone of inhibition ranged from 9.0 to 26.0 mm at 100 mg/mL for all the plant parts. The ethyl acetate leaf extract of this plant possessed the highest antibacterial activity with MIC and MBC values of the range of (3.125 to ˃50 mg/mL) and (12.5 to ˃50 mg/mL) respectively. The zone inhibition of the chromatographic fractions of both plants ranged 15-23 mm. Antioxidant study of the extracts of the leaf of L. egregia revealed that the ethyl acetate and methanol extracts have good antioxidant potentials comparable to that of ascorbic acid control. This study has revealed that the extracts from different parts of L. egregia possess good antibacterial and antioxidant activities which could be a function of the various phytochemicals detected in the plant. Keywords: Lannea egregia, Phytochemical, Antibacterial, Antioxidant, Column chromatography.

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