scholarly journals Phytochemical, Antioxidant and Antimicrobial studies of Lannea egregia Engl. & K. Krause (Anacardiaceae) extracts and chromatographic fractions

2020 ◽  
Vol 19 (1) ◽  
pp. 348-363
Author(s):  
Philip A. Idowu ◽  
Loveth C. Ekemezie ◽  
Charles O. Olaiya
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Antioxidant Capacity ◽  
Reducing Power ◽  
Stem Bark ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Crude Extracts ◽  
Different Parts

Screening ‘new’ medicinal plants of traditional importance for bioactive components is a sure way of discovering novel therapeutic agents to treat diseases. This study, therefore investigated the presence of phytochemical, antioxidant and antibacterial components of the extracts of Lannea egregia. Phytochemical screening was done by standard methods. Antibacterial activity of the extracts of Lannea egregia was determined by agar well diffusion method while the minimum inhibitory concentration (MIC) was determined by agar dilution method. The antioxidant capacity of the crude extracts was determined through the evaluation of total flavonoid content, total phenolic content, ferric reducing power, total antioxidant capacity and 2,2-diphenyl-1-picryhydrazyl. The phytochemical screening of the different parts of this plant revealed the presence of tannins, saponins, alkaloids, flavonoids, steroids, terpenoids, emodins, phlobatannins, anthocyanins, coumarins and phenolics. Phlobatannins was observed to be absent in the stem bark. The crude extracts obtained from the leaves, stem bark and roots of this plant exhibited good antibacterial activity against typed strains of Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa and Escherichia coli. The diameter of the zone of inhibition ranged from 9.0 to 26.0 mm at 100 mg/mL for all the plant parts. The ethyl acetate leaf extract of this plant possessed the highest antibacterial activity with MIC and MBC values of the range of (3.125 to ˃50 mg/mL) and (12.5 to ˃50 mg/mL) respectively. The zone inhibition of the chromatographic fractions of both plants ranged 15-23 mm. Antioxidant study of the extracts of the leaf of L. egregia revealed that the ethyl acetate and methanol extracts have good antioxidant potentials comparable to that of ascorbic acid control. This study has revealed that the extracts from different parts of L. egregia possess good antibacterial and antioxidant activities which could be a function of the various phytochemicals detected in the plant. Keywords: Lannea egregia, Phytochemical, Antibacterial, Antioxidant, Column chromatography.

scholarly journals Evaluation of Antioxidant and Antibacterial Properties of Magnolia champaca L (Magnoliaceae) Stem Bark Extract

2020 ◽  
Vol 23 (2) ◽  
pp. 96-102
Author(s):  
Md Mahadi Hasan ◽  
Masuma Akter ◽  
Md Ekramul Islam ◽  
Md Aziz Abdur Rahman ◽  
Mst Shahnaj Parvin
Keyword(s):  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Antibacterial Properties ◽  
Reducing Power ◽  
Stem Bark ◽  
Total Antioxidant ◽  
Pharmacologically Active

The current study was designated to explore the antioxidant and antibacterial properties of the methanolic extract of Magnolia champaca stem bark and its different fractions. Antioxidant activity was assessed using total antioxidant capacity, ferric reducing power, DPPH, hydroxyl and hydrogen peroxide scavenging assay. Antibacterial activity was evaluated against five gram positive and five gram negative bacteria using disc diffusion assay method. Among the different fractions, chloroform fraction (CHF) and ethyl acetate fraction (EAF) showed the highest antioxidant activity whereas aqueous fraction (AQF) showed lowest activity in DPPH radical scavenging assay with IC50 of 12.12, 22.41 and 55.16 μg/ml, respectively. Both of the extracts CHF and EAF also exhibited highest total antioxidant capacity, ferric reducing power and hydrogen peroxide scavenging activity with concentration dependent manner when compared to standard BHT. Moderate to potent antibacterial activity was observed against all tested organisms compared to standard azithromycin. The results from the present study revealed that the different fractions of stem bark of M. champaca specially CHF and EAF possess antioxidant and antibacterial property which support its use in traditional medicine and suggesting that the plant may be further investigated to discover its pharmacologically active natural products. Bangladesh Pharmaceutical Journal 23(2): 96-102, 2020

scholarly journals Analysis of the Active Constituents and Evaluation of the Biological Effects of Quercus acuta Thunb. (Fagaceae) Extracts

Molecules ◽  
2018 ◽  
Vol 23 (7) ◽  
pp. 1772 ◽  
Author(s):  
Mi-Hyeon Kim ◽  
Dae-Hun Park ◽  
Min-Suk Bae ◽  
Seung-Hui Song ◽  
Hyung-Ju Seo ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Antibiotic Resistant ◽  
Mrsa Strains ◽  
Quercus Acuta

We evaluated the antioxidant and antibacterial activity of hexnane, ethyl acetate, acetone, methanol, ethanol, and water extracts of the Quercus acuta leaf. The antioxidant properties were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, reducing power, and total phenolic content. Antibacterial activity was assessed against general infectious pathogens, including antibiotic-resistant clinical isolates. The methanolic extract showed the highest DPPH radical scavenging activity and total phenolic content, while the reducing power was the highest in the water extract. The ethyl acetate extract showed the best antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA) strains. Additionally, it displayed antibacterial activity against Staphylococcus aureus KCTC1928, Micrococcus luteus ATCC 9341, Salmonella typhimurium KCTC 1925, Escherichia coli KCTC 1923, and eight MRSA strains. These results present basic information for the possible uses of the ethanolic and ethyl acetate extracts from Q. acuta leaf in the treatment of diseases that are caused by oxidative imbalance and antibiotic-resistant bacterial infections. Six active compounds, including vitamin E, which are known to possess antioxidant and antibacterial activity, were identified from the extracts. To the best of our knowledge, this is the first study that reports the chemical profiling and antibacterial effects of the various QA leaf extracts, suggesting their potential use in food therapy or alternative medicine.

scholarly journals Caralluma tuberculata N.E.Br Manifests Extraction Medium Reliant Disparity in Phytochemical and Pharmacological Analysis

Molecules ◽  
2021 ◽  
Vol 26 (24) ◽  
pp. 7530
Author(s):  
Muhammad Waleed Baig ◽  
Madiha Ahmed ◽  
Nosheen Akhtar ◽  
Mohammad K. Okla ◽  
Bakht Nasir ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Ethyl Acetate ◽  
Antioxidant Capacity ◽  
Leukemia Cell ◽  
Reducing Power ◽  
Acetone Extract ◽  
In Vitro Assays ◽  
Total Phenolic ◽  
Human Leukemia ◽  
Distilled Water

Solubility of phytoconstituents depends on the polarity of the extraction medium used, which might result in the different pharmacological responses of extracts. In line with this, ethnomedicinally important food plant (i.e., Caralluma tuberculata extracts) have been made in fourteen distinct solvent systems that were then analyzed phytochemically via total phenolic amount estimation, total flavonoid amount estimation, and HPLC detection and quantification of the selected polyphenols. Test extracts were then subjected to a battery of in vitro assays i.e., antioxidants (DDPH scavenging, antioxidant capacity, and reducing power estimation), antimicrobial (antibacterial, antifungal, and antileishmanial), cytotoxic (brine shrimps, THP-1 human leukemia cell lines and normal lymphocytes), and protein kinase inhibition assays. Maximum phenolic and flavonoid contents were computed in distilled water–acetone and acetone extracts (i.e., 16 ± 1 μg/mg extract and 8 ± 0.4/mg extract, respectively). HPLC-DAD quantified rutin (0.58 µg/mg extract) and gallic acid (0.4 µg/mg extract) in methanol–ethyl acetate and methanol extracts, respectively. Water–acetone extract exhibited the highest DPPH scavenging of 36 ± 1%. Total reducing potential of 76.0 ± 1 μg/mg extract was shown by ethanol chloroform while maximum total antioxidant capacity was depicted by the acetone extract (92.21 ± 0.70 μg/mg extract). Maximal antifungal effect against Mucor spp., antileishmanial, brine shrimp cytotoxicity, THP-1 cell line cytotoxicity, and protein kinase inhibitory activities were shown by ethyl acetate-methanol (MIC: 50 µg/disc), n-hexane (IC50: 120.8 ± 3.7 µg/mL), ethyl acetate (LD50: 29.94 ± 1.6 µg/mL), distilled water–acetone (IC50: 118 ± 3.4 µg/mL) and methanol–chloroform (ZOI: 19 ± 1 mm) extracts, respectively. Our findings show the dependency of phytochemicals and bioactivities on the polarity of the extraction solvent and our preliminary screening suggests the C. tuberculata extract formulations to be tested and used in different ailments, however, detailed studies remain necessary for corroboration with our results.

scholarly journals Skrining Fitokimia dan Uji Aktivitas Antibakteri Fraksi Etil Asetat dan n-Heksana dari Daun Mangga Kasturi (Mangifera casturi Kosterm.)

KOVALEN ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 227-233
Author(s):  
Dwi Lestari ◽  
Desy Fitriani ◽  
Serli Anngraeni
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Propionibacterium Acnes ◽  
Ethyl Acetate Fraction ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Disk Diffusion Method ◽  
Study Results ◽  
Mango Leaves ◽  
Weak Antibacterial Activity

Kasturi mango (Mangifera casturi Kosterm.) is a typical mango of South Kalimantan. Kasturi mango leaves are reported to have antioxidant activity and are potential for treating various diseases, including diseases related to antibacterial. This study examines the antibacterial activity of ethyl acetate fraction and the n-hexane fraction of mango musk leaves on bacteria that cause acne. Experimental research started with plant determination, making extracts and fractions, phytochemical screening, and antibacterial testing against Staphylococcus aureus and Propionibacterium acnes using the disk diffusion method. The study results found that the ethyl acetate and n-hexane fractions had weak antibacterial activity against S. aureus and P. acnes bacteria, which cause acne

scholarly journals EVALUATION OF BIOACTIVITY OF JAGGERY PREPARED USING PLANT MUCILAGE AS CLARIFICANT

2018 ◽  
Vol 11 (11) ◽  
pp. 294
Author(s):  
Harish Nayaka Ma ◽  
Lava Chikkappaiah ◽  
Venkatesh Ks ◽  
Gunashree Bs ◽  
Sudharshan S
Keyword(s):  
Antibacterial Activity ◽  
Aloe Vera ◽  
Reducing Power ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Total Phenol Content ◽  
Total Flavonoids ◽  
Death Time ◽  
Reducing Power Assay

Objective: The aim of the study was to prepare the jaggery using plant mucilage as clarificants and to evaluate the bioactivity of jaggery by determining total phenols, total flavonoids, reducing power, antibacterial activity, and antihelmintic activity.Methods: Jaggery was prepared from sugarcane variety Co-80632 using five plant mucilage as clarificants, namely Aloe vera, flax seeds, fenugreek, purslane, and malabar spinach at three different concentrations, i.e., 0.1%, 0.2%, and 0.4% of raw sugarcane juice. The characteristic bioactivities such as total phenol content by Folin–Ciocalteu method, flavonoids content by spectrophotometer method, reducing power assay by measuring the absorbance at 700 nm, antibacterial activity by well-diffusion method, and antihelmintic activity by in vitro were determined.Results: The highest total phenolic content was observed in jaggery prepared using plant mucilage clarificant of A. vera (JAV4 [3.68±0.02]) and Fenugreek seeds (JFG4 [3.61±0.02]) at 0.4% followed by them at 0.2% and 0.1% concentration, respectively. Similarly, a significant amount of total flavonoids content was elevated only in 0.4% plant mucilage clarificants of Aloe vera-treated experimental group jaggery (0.73±0.01) compared to control (0.61±0.02). Further, the antioxidant activity of jaggery was evaluated by reducing power. The increased absorbance at 700 nm indicated the presence of reducing power. At 10 mg/ml concentration, an absorbance unit of 1.93 and 1.80 was observed for jaggery prepared using 0.4% concentration of A. vera and fenugreek seed mucilage clarificants. Antihelmintic activity of jaggery showed dosage depended on the pattern where with the increase in the concentration of mucilage, the antihelmintic activity enhanced. A. vera mucilage showed maximum antihelmintic activity at 0.4% concentration with paralytic time (28.6 min) and death time (39.6 min).Conclusion: Jaggery prepared using plants mucilaginous clarificants is the richest source of polyphenols such as phenolic acids and flavonoids, thereby contributed to the enhancement of bioactivity such as antioxidant, antibacterial, and antihelmintic activity. Thus, jaggery prepared using plant mucilage as clarificants is a novel nutraceutical product which can supplement the nutrients along with various medicinal properties through antioxidant, antibacterial, and antihelmintic activity.

scholarly journals Metabolomic Profiling and Antioxidant Activities of Breonadia salicina Using 1H-NMR and UPLC-QTOF-MS Analysis

Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6707
Author(s):  
Dorcas B. Tlhapi ◽  
Isaiah D. I. Ramaite ◽  
Chinedu P. Anokwuru
Keyword(s):  
Antioxidant Activity ◽  
1H Nmr ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Stem Bark ◽  
Free Radical Scavenging ◽  
Caffeoylquinic Acid ◽  
Crude Extracts ◽  
Different Parts

Breonadia salicina (Vahl) Hepper and J.R.I. Wood is widely used in South Africa and some other African countries for treatment of various infectious diseases such as diarrhea, fevers, cancer, diabetes and malaria. However, little is known about the active constituents associated with the biological activities. This study is aimed at exploring the metabolomics profile and antioxidant constituents of B. salicina. The chemical profiles of the leaf, stem bark and root of B. salicina were comprehensively characterized using proton nuclear magnetic resonance (1H-NMR) spectroscopy and ultra-performance liquid chromatography with quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS). The antioxidant activities of the crude extracts, fractions and pure compounds were determined using the DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging and reducing power assays. A total of 25 compounds were tentatively identified using the UPLC-QTOF-MS. Furthermore, the 1H-NMR fingerprint revealed that the different parts of plant had differences and similarities among the different crude extracts and fractions. The crude extracts and fractions of the root, stem bark and leaf showed the presence of α-glucose, β-glucose, glucose and fructose. However, catechin was not found in the stem bark crude extracts but was found in the fractions of the stem bark. Lupeol was present only in the root crude extract and fractions of the stem bark. Furthermore, 5-O-caffeoylquinic acid was identified in the methanol leaf extract and its respective fractions, while the crude extracts and fractions from the root and dichloromethane leaf revealed the presence of hexadecane. Column chromatography and preparative thin-layer chromatography were used to isolate kaempferol 3-O-(2″-O-galloyl)-glucuronide, lupeol, d-galactopyranose, bodinioside Q, 5-O-caffeoylquinic acid, sucrose, hexadecane and palmitic acid. The crude methanol stem bark showed the highest antioxidant activity in the DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging activity with an IC50 value of 41.7263 ± 7.6401 μg/mL, whereas the root crude extract had the highest reducing power activity with an IC0.5 value of 0.1481 ± 0.1441 μg/mL. Furthermore, the 1H-NMR and UPLC-QTOF-MS profiles showed the presence of hydroxycinnamic acids, polyphenols and flavonoids. According to a literature survey, these phytochemicals have been reported to display antioxidant activities. Therefore, the identified hydroxycinnamic acid (caffeic acid), polyphenol (ellagic acid) and flavonoids (catechin and (epi) gallocatechin) significantly contribute to the antioxidant activity of the different parts of plant of B. salicina. The results obtained in this study provides information about the phytochemistry and phytochemical compositions of Breonadia salicina, confirming that the species is promising in obtaining constituents with medicinal potential primarily antioxidant potential.

scholarly journals In vitro Antioxidant Properties of Bersama abyssinica Stem Bark Extracts

Proceedings ◽  
2019 ◽  
Vol 40 (1) ◽  
pp. 21
Author(s):  
Kouadio Ibrahime Sinan ◽  
Kouadio Bene ◽  
Abdurrahman Aktumsek ◽  
Gokhan Zengin
Keyword(s):  
Ethyl Acetate ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Water Extract ◽  
Reducing Power ◽  
Stem Bark ◽  
Sub Saharan Africa ◽  
Total Phenolic ◽  
Bersama Abyssinica

Bersama abyssinica, belonging to the Melianthaceae family, is distributed across Sub Saharan Africa. Decoctions of the bark, leaves, and roots of B. abyssinica have been extensively used in traditional medicine to manage many stomach complications such as colic, diarrhea, dysentery, and intestinal worms. In this study, we examined three extracts (ethyl acetate, methanol and water) obtained from B. abyssinica stem barks in terms of antioxidant properties. The antioxidant abilities were investigated by different chemical methods, including free radical scavenging (DPPH and ABTS), reducing power (CUPRAC and FRAP), metal chelating and phosphomolybdenum assay. In addition, total phenolic and flavonoid contents in the extracts were calculated. The highest level of phenolics was determined in water extract (230.83 mg GAE/g extract), followed by methanol (216.79 mg GAE/g extract) and ethyl acetate (100.57 mg GAE/g extract). In same line with total phenolic content, the best antioxidant properties were noted for water and methanol extracts. Our findings suggested that B. abyssinica stem bark extracts could be considered as promising sources of natural antioxidants.

scholarly journals PHYTOCHEMICAL SCREENING AND PHARMACOLOGICAL EVALUATION OF DIFFERENT EXTRACTS OF PLANT CASEARIA TOMENTOSA LEAVES

2017 ◽  
Vol 9 (4) ◽  
pp. 166
Author(s):  
Ritu Tyagi ◽  
Abha Shukla ◽  
Rishi Kumar Shukla
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Klebsiella Pneumonia ◽  
Phytochemical Screening ◽  
Antioxidant Power

Objective: In present study different extracts of the leaves of plant Casearia tomentosa was investigated for the phytochemical screening, total phenolic content and various pharmacological activities like antioxidant, antidiabetic, antibacterial.Methods: Leaves of C. tomentosa were successively extracted by soxhlet extraction method using petroleum ether, chloroform, ethyl acetate and ethanol. The preliminary phytochemical investigation of different extracts was carried out according to standard methods. Antioxidant activity evaluated by 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant potential (FRAP) method. Total phenolic content was evaluated by Folin-ciocalteau method. Antidiabetic activity estimated by α-amylase and α-glucosidase inhibitory assay while antibacterial activity was performed by disk diffusion method.Results: C. tomentosa leaves show the presence of various phytochemicals like alkaloids, flavonoids, carbohydrates, glycosides, protein etc. Results of antioxidant studies revealed that the ethanol extract possessed highest 2, 2-diphenyl-1-picryl-hydrazyl (IC50 31.87±0.65 μg/ml) activity, ferric reducing antioxidant power (43.78±0.65μM/ml, FRAP value=2.30). Among different extracts, ethanol extract shows highest (228.17±0.63 mg gallic acid equivalents (GAE)/gram of dry mass) amount of total phenolic content. The ethyl acetate extract exhibited highest inhibition on α-amylase (IC50 262.08±1.26 μg/ml) and α-glucosidase (IC50 459.25±1.80 μg/ml) enzymes. Results of antibacterial activity revealed that all extracts show inhibition against selected bacterial strains like Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia and Salmonella typhi.Conclusion: The present study suggests that Casearia tomentosa leaves can be used as a potential source of natural antioxidant and natural antidiabetic, antibacterial agents.

scholarly journals A STUDY ON PHYTOCHEMICAL SCREENING, ANTIOXIDANT, ANTIMICROBIAL AND α-ΑMYLASE INHIBITORY ACTIVITIES OF CRUDE EXTRACTS OF THE STEM BARK OF PISONIA GRANDIS, R.BR

2017 ◽  
Vol 10 (11) ◽  
pp. 129
Author(s):  
Vishu Mohan ◽  
Mohan Kumar Ramasamy ◽  
Srikalyani Vemuri ◽  
Ilango Kaliappan
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Stem Bark ◽  
Antidiabetic Activity ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Flavonoid Content ◽  
Phenolic And Flavonoid

  Objective: The aim of the present study is to determine the phytochemical screening, antioxidant activity and α-amylase inhibitory activity of the crude hexane, ethyl acetate and ethanolic stem bark extract of Pisonia grandis.Methods: The evaluation of antioxidant and antimicrobial activity, total phenolic, and flavonoid content were assessed using 2,2-diphenyl-1- picrylhydrazyl, Folin–Ciocalteu’s reagent, and aluminum chloride assay, respectively. The antidiabetic activity was assessed for porcine pancreatic α-amylase for the stem bark of P. grandis. Results: Phytochemical screening confirmed the presence of phenolic, flavonoids, tannins, saponins, terpenoids, and steroids in all the three extracts. The antioxidant activity showed 148.2 μg/ml, total phenolic content (gallic acid equivalent), 0.0665±0.0002 mg/g, flavonoid content (quercetin equivalent), 0.6061±0.1817 mg/g, and inhibitory concentration 50% values were found to be 40.42 μg/ml and showed better in ethyl acetate extract. The antidiabetic activity exhibited mimic action with insulin due to the presence of pinnatol in the stem bark and leaves of P. grandis. Conclusion: P. grandis stem bark crude ethyl acetate extract showed strong antioxidant activity, high phenolic, and flavonoid content. The antimicrobial activity was studied in both Gram-positive and Gram-negative strains against ampicillin and rifampicin as reference drugs. Antidiabetic activity shows effective result by α-amylase inhibitory activity.

scholarly journals Study on Biological Activity of Litsea monopetala From Panchthar District of Nepal

2020 ◽  
Vol 25 (2) ◽  
pp. 113-118
Author(s):  
Subash Khanal ◽  
Bishan Datt Bhatt
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Bioactive Substances ◽  
Ic50 Values ◽  
Acetone Extracts ◽  
Dpph Radical Scavenging

The Panchthar, a hilly district of Eastern Nepal, has several medicinal plants which are used by indegenous people for the treatments of different diseases. In the present work, phytochemical and biological activities of hexane, ethyl acetate, acetone, and methanol extracts of Litsea monopetala leaves from the Pachthar district were analyzed. The phytochemical screening demonstrated the presence of major secondary metabolites like flavonoids, alkaloids, terpenoids, polyphenols, glycosides, and proteins. The antibacterial activity was estimated by the disc diffusion method against Bacillus substilus and Escherichia coli. The result showed that ethyl acetate extract exhibit the highest zone of inhibition (ZOI) value of 18 mm/disc. Acetone extract also showed significant antibacterial activity at the concentration of 100 mg/mL. Antioxidant activity from DPPH radical scavenging assay displayed the inhibitory concentration (IC50) values of dried methanol and acetone extracts were 30.74 μg/mL and 202.86 μg/mL, respectively. The methanol extract has the highest total phenolic content of 360.14 mg GAE/g extract and the highest total flavonoid content of 27.13 mg QE/g extract. The present study revealed that Litsea monopetala plant from the Panchthar district is a source of several bioactive substances and can be used in several drugs formulation.

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