Antioxidant activity of Tinospora cordifolia leaf extracts through non-enzymatic method

Background: Medicinal plants are considered a rich source of ingredients, which can be used in drug development and synthesis. Tinospora cordifolia (Wild.) Hook.f. & Thomson, commonly known as guduchi, heart-leaved moonseed and giloya is a herbaceous vine of the family Menispermaceae, has several beneficial properties including antioxidant activity. Aim: The present study was carried out to analyze the antioxidant activity of leaf and stem extracts of Tinospora cordifolia by using DPPH (1,1-Diphenyl-2-picrylhydrazyl) and ABTS (2,2´-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)) free radical scavenging assays. Materials and Methods: Dried and powdered leaves and stem of T. cordifolia were extracted with methanol. Ascorbic acid was taken as standard. Total phenolic content was estimated by using Folin-ciocalteu's reagent while total flavonoid content by aluminium chloride reagent to find the correlation of polyphenols with antioxidant activity. ABTS assay of methanolic leaf and stem extracts showed the highest scavenging activity as compared to the DPPH assay. Results: Methanolic stem extract showed higher phenolic and flavonoid content along with antioxidant activity as compared to the methanolic leaf extract. Conclusion: The stem extract exhibited more antioxidant activity than the leaf extract with regards to the all parameters analyzed.

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Antimicrobial and antioxidant activity of the crude leaf extracts of Pycnostachys urticifolia Hook. (Lamiaceae)

Planta Medica ◽

10.1055/s-0035-1565645 ◽

2015 ◽

Vol 81 (16) ◽

Author(s):

JR Naidoo ◽

Y Naidoo ◽

J Van Staden

Keyword(s):

Antioxidant Activity ◽

Leaf Extracts ◽

Antimicrobial And Antioxidant Activity

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Characterization of Phytoconstituents from Alcoholic Extracts of Four Woody Species and Their Potential Uses for Management of Six Fusarium oxysporum Isolates Identified from Some Plant Hosts

Plants ◽

10.3390/plants10071325 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1325

Author(s):

Mohamed Z. M. Salem ◽

Abeer A. Mohamed ◽

Hayssam M. Ali ◽

Dunia A. Al Al Farraj

Keyword(s):

Antioxidant Activity ◽

Free Radical ◽

Benzoic Acid ◽

Fusarium Oxysporum ◽

Leaf Extract ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Bark Extract ◽

Leaf Extracts ◽

Conium Maculatum

Background: Trees are good sources of bioactive compounds as antifungal and antioxidant activities. Methods: Management of six molecularly identified Fusarium oxysporum isolates (F. oxy 1, F. oxy 2, F. oxy 3, F. oxy 4, F. oxy 5 and F. oxy 6, under the accession numbers MW854648, MW854649, MW854650, MW854651, and MW854652, respectively) was assayed using four extracts from Conium maculatum leaves, Acacia saligna bark, Schinus terebinthifolius wood and Ficus eriobotryoides leaves. All the extracts were analyzed using HPLC-VWD for phenolic and flavonoid compounds and the antioxidant activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and β-carotene-linoleic acid (BCB) bleaching assays. Results: In mg/kg extract, the highest amounts of polyphenolic compounds p-hydroxy benzoic, benzoic, gallic, and rosmarinic acids, with 444.37, 342.16, 311.32 and 117.87, respectively, were observed in C. maculatum leaf extract; gallic and benzoic acids with 2551.02, 1580.32, respectively, in A. saligna bark extract; quinol, naringenin, rutin, catechol, and benzoic acid with 2530.22, 1224.904, 798.29, 732.28, and 697.73, respectively, in S. terebinthifolius wood extract; and rutin, o-coumaric acid, p-hydroxy benzoic acid, resveratrol, and rosmarinic acid with 9168.03, 2016.93, 1009.20, 1156.99, and 574.907, respectively, in F. eriobotryoides leaf extract. At the extract concentration of 1250 mg/L, the antifungal activity against the growth of F. oxysporum strains showed that A. saligna bark followed by C. maculatum leaf extracts had the highest inhibition percentage of fungal growth (IPFG%) against F. oxy 1 with 80% and 79.5%, F. oxy 2 with 86.44% and 78.9%, F. oxy 3 with 86.4% and 84.2%, F. oxy 4 with 84.2, and 82.1%, F. oxy 5 with 88.4% and 86.9%, and F. oxy 6 with 88.9, and 87.1%, respectively. For the antioxidant activity, ethanolic extract from C. maculatum leaves showed the lowest concentration that inhibited 50% of DPPH free radical (3.4 μg/mL). Additionally, the same extract observed the lowest concentration (4.5 μg/mL) that inhibited BCB bleaching. Conclusions: Extracts from A. saligna bark and C. maculatum leaves are considered potential candidates against the growth of F. oxysporum isolates—a wilt pathogen—and C. maculatum leaf as a potent antioxidant agent.

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In vitro anti-bacterial activity of Tinospora cordifolia leaf extract and its phytochemical screening

Journal of Biomedical Sciences ◽

10.3126/jbs.v5i2.23633 ◽

2019 ◽

Vol 5 (2) ◽

pp. 10-17

Author(s):

B. Praiwala ◽

S. Priyanka ◽

N. Raghu ◽

N. Gopenath ◽

A. Gnanasekaran ◽

...

Keyword(s):

Cell Division ◽

Leaf Extract ◽

Antibacterial Property ◽

Standard Procedure ◽

Tinospora Cordifolia ◽

Diffusion Method ◽

Main Concern ◽

Phytochemical Screening ◽

Leaf Extracts ◽

Screening Assay

Background: Antimicrobial resistance is the main concern worldwide to combat infectious. Over the years studies on leaf extracts Tinospora cordifolia have demonstrated the potent role its antibacterial property. The current study is an attempt to test its antibacterial property against Escherichia coli cell division. Material and methods: Phytochemical screening assay of T. cordifolia leaf extract was done using standard procedure and the results showed the presence of alkaloid, carbohydrate, terpenoid, steroid, tannin, amino acid, flavonoid and glycoside components. Results: HPLC analysis revealed the presence of berberine in T. cordifolia leaf extract. Further E. coli cells were treated with berberine to study its efficacy in inhibiting cell division. Antibacterial assay was performed by using disc diffusion method. Conclusion: Among aqueous, methanolic, ethanolic, chloroform, hexane and acetone extract only methanolic extract showed zone of inhibition.

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FRACTIONATION OF THE N-HEXANE EXTRACT OF GARCINIA BANCANA MIQ. (MANGGIS HUTAN) LEAVES AND ITS ANTIOXIDANT ACTIVITY BASED ON 1,1-DIPHENYL-2- PICRYLHYDRAZYL AND FERRIC REDUCING ANTIOXIDANT POWER ASSAYS

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2018.v10s1.90 ◽

2018 ◽

Vol 10 (1) ◽

pp. 407

Author(s):

Dewi Kumala Putri ◽

Berna Elya ◽

Nuraini Puspitasari

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Hexane Extract ◽

Phytochemical Screening ◽

Active Fraction ◽

Leaf Extracts ◽

Antioxidant Power ◽

Dpph Method ◽

Ferric Reducing Antioxidant Power

Objective: To assess the antioxidant activity from another part of the plant, in this study, leaf extracts in n-hexane were fractionated.Methods: Ten fractions were obtained and tested in vitro for antioxidant activity using two methods, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferricreducing antioxidant power (FRAP), to identify the most active fraction.Results: The IC50 of the most active fraction was 36.24 μg/mL using the DPPH method, and the EC50 was 39.54 μg/mL using the FRAP method. Themost active fraction was also shown to contain terpenoids.Conclusion: The most active fraction of an n-hexane extract of the leaves of Gacinia bancana Miq., which was tested by both DPPH and FRAP methodshad antioxidant activities with IC50 and EC50 values of 36.2482 μg/mL and 39.5442 μg/mL, respectively. Phytochemical screening showed that activefraction contains terpenoids.

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Determination of Chemical Constituents and Antioxidant Activities of Leaves and Stems from Jatropha cinerea (Ortega) Müll. Arg and Jatropha cordata (Ortega) Müll. Arg

Plants ◽

10.3390/plants10020212 ◽

2021 ◽

Vol 10 (2) ◽

pp. 212

Author(s):

Yeimi Cecilia Vega-Ruiz ◽

Corina Hayano-Kanashiro ◽

Nohemí Gámez-Meza ◽

Luis Angel Medina-Juárez

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Biological Effects ◽

Chemical Constituents ◽

Weather Conditions ◽

Antioxidant Compounds ◽

Leaf Extracts ◽

Methanolic Extracts ◽

Northwestern Region ◽

High Concentrations

Jatropha species have been shown to be an important source of secondary metabolites with different biological effects. Jatropha cinerea (Ortega) Müll. Arg and Jatropha cordata (Ortega) Müll. Arg are distributed in the Northwestern region of Mexico, are adapted to extreme weather conditions and are widely used (stems, leaves, and sap) in traditional medicine. The aim of the present study was to carry out the phytochemical characterization and the evaluation of the antioxidant activity in methanolic extracts of stems and leaves from J. cinerea and J. cordata. The compounds present in the extracts of both species were characterized by ESI-IT-MS/MS and quantified by HPLC-DAD. The results showed that the stem extracts of both species are rich in phenolic acids, while the leaf extracts are rich in flavonoids. Some of the main compounds found were gallic acid, gentisic acid, 3,4-Dihydroxybenzoic acid, vitexin, isovitexin, and catechol. Both species showed high concentrations of phenols and total flavonoids and antioxidant activity. J. cordata showed the highest antioxidant capacity and the highest concentration of phenolic compounds. Overall, both Jatropha species are a natural source of antioxidant compounds with potential biotechnological uses.

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Phytochemical Analysis, Antioxidant Activity and In Vitro Growth Inhibition of Struvite Crystals by I pomoea Eriocarpa Leaf Extracts

Journal of Food Biochemistry ◽

10.1111/jfbc.12205 ◽

2015 ◽

Vol 40 (2) ◽

pp. 148-160 ◽

Cited By ~ 6

Author(s):

Moonjit Das ◽

Himaja Malipeddi ◽

N. Arunai Nambiraj ◽

Reshma Rajan

Keyword(s):

Antioxidant Activity ◽

Growth Inhibition ◽

Phytochemical Analysis ◽

In Vitro Growth ◽

Leaf Extracts

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Identification of phytoconstituents of Tragia involucrata leaf extracts and evaluate their correlation with anti-inflammatory & antioxidant properties

Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry ◽

10.2174/1871523020666210126144506 ◽

2021 ◽

Vol 20 ◽

Author(s):

Vinodhini Velu ◽

Swagata Banerjee ◽

Vidya Rajendran ◽

Gaurav Gupta ◽

Dinesh Kumar Chellappan ◽

...

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Petroleum Ether ◽

Ethyl Acetate Extract ◽

Petroleum Ether Extract ◽

Inflammatory Activity ◽

Ether Extract ◽

Membrane Stabilization ◽

Leaf Extracts ◽

Anti Inflammatory

Aims: The present investigation was aimed at exploring the phytoconstituents using Gas Chromatography Mass Spectroscopy and to evaluate antioxidant and anti-inflammatory properties of the leaf extracts. Materials and Methods: The extracts were obtained sequentially with petroleum ether, ethyl acetate and water using soxhlet apparatus. The anti-inflammatory property of the identified compounds using GC- MS spectroscopy was evaluated in silico. The antioxidant activity was performed by DPPH and H2O2 method whereas anti-inflammatory study was carried out by HRBC membrane stabilization method. Terpenoids were found to be major constituents in petroleum ether extract while, phenols and flavonoids were predominantly found in ethyl acetate extract. Results and Discussion: The GC-MS analysis of the extract revealed six major molecules including Squalene, 19β, 28- epoxyleanan-3-ol and 2-tu-Butyl-5-chloromethyl-3-methyl-4-oxoimidazolidine-1-carboxylic acid. The ethyl acetate extract showed a significant antioxidant activity (P<0.01) in both DPPH method (70.87 %) and H2O2 method (73.58%) at 200 µg mL-1 . Increased membrane stabilization of petroleum ether extract was observed in the in vitro anti-inflammatory activity study. A strong relationship between the terpenoid content and anti-inflammatory activity was obtained from the correlation (0.971) and docking study. Conclusion: These results justify T. involucrata to be a rich source of terpenoids with potent antiinflammatory property.

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EFFECT OF DIFFERENT SOLVENTS ON ANTIOXIDANT ACTIVITY OF LEAF EXTRACTS OF CALOTROPIS PROCERA AND AZADIRACHTA INDICA

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i1.15145 ◽

2016 ◽

Vol 10 (1) ◽

pp. 268

Author(s):

Sharmistha Banerjee ◽

Shuchi Kaushik ◽

Rajesh Singh Tomar

Keyword(s):

Antioxidant Activity ◽

Total Phenolics ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Calotropis Procera ◽

Peroxide Radical ◽

Scavenging Activity ◽

Leaf Extracts ◽

Dry Extract ◽

Leaf Powder

ABSTRACTObjective: This study was performed to identify the phytochemicals and comparatively evaluate the antioxidant activity of Calotropis procera andAzadirachta indica by detection of total phenolics, hydrogen peroxide radical scavenging activity, and estimation of condensed tannins in differentsolvent systems and at different temperatures.Methods: Leaves of C. procera and A. indica were extracted in water, methanol by soaking dried leaf powder at room temperature and also by boilingthe leaf powder in water for 30 minutes. Phytochemical tests were performed in all of the extracts. The antioxidant activity was determined byhydrogen peroxide radical scavenging activity. Quantitative estimation of total phenolics and hydrolysable tannins was also performed.Results: The total phenolics in both leaf extracts was obtained maximum in boiled extract (40.7±1.20 mg gallic acid equivalent [GAE]/g dry extractin C. procera and 33.66±1.45 mg GAE/g dry extract in A. indica). The amount of hydrolysable tannins in both leaf extracts was found to be highest inmethanol (150±1.88 mg catechin equivalent/g dry extract in C. procera and 144.8±2.63 mg catechin equivalent/g dry extract in A. indica).Conclusion: The study showed promising results indicating that these plants are a good source of antioxidants. The majority of phytochemicals wereextracted in distilled water and methanol acts as a good solvent for extraction of tannins, whereas an increase in temperature leads to poor extractionof tannins.Keywords: Antioxidant, Phytochemicals, Phenolics, Radical, Tannins.© 2017 The Authors. Published by Innovare Academic Sciences Pvt Ltd. This is an open access article under the CC BY license (http://creativecommons.

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