Characterization of the bioactive constituents of Nymphaea alba rhizomes and evaluation of anti-biofilm as well as antioxidant and cytotoxic properties

Understanding the bioactive constituents and physicochemical components in cereals can provide insights into their potential health benefits and food applications. This study evaluated some bioactive constituents, carbohydrate profiles and pasting properties of 16 Striga-resistant hybrids, with yellow-orange kernel color and semi-flint to flint kernel texture, grown in two replications at two field locations in Nigeria. Carotenoids were quantified using HPLC, while other analyses were carried out using standard laboratory methods. The ranges of major carotenoids (μg/g) across the two locations varied from 2.6 to 9.6 for lutein, from 2.1 to 9.7 for zeaxanthin, from 0.8 to 2.9 for β-cryptoxanthin, from 1.4 to 4.1 for β-carotene; with total xanthophylls and provitamin A carotenoids (pVAC) ranging from 5.4 to 17.1 and 1.4 to 4.1 μg/g, respectively. Tannins content ranged from 2.1 to 7.3 mg/g, while phytate ranged from 0.4 to 7.1%. Starch, free sugar, amylose and amylopectin ranged from 40.1 to 88.9%, 1.09 to 6.5%, 15.0 to 34.1%, and 65.9 to 85.0%, respectively. Peak and final viscosities ranged from 57.8 to 114.9 and 120.3 to 261.6 Rapid Visco Units (RVU), respectively. Total xanthophylls, β-carotene, tannins, phytate, sugar, amylose and amylopectin levels, as well as peak and final viscosities, varied significantly (p < 0.05) across the hybrids. Amylose was significantly correlated (p < 0.05) with total xanthophylls, β-carotene, pVAC, phytate and pasting temperature (r = 0.3, 0.3, 0.4, 0.3, 0.3, respectively), but starch significantly correlated with tannins (r = 0.3). Hence, the Striga-resistant yellow-orange maize hybrids have a good combination of bioactive constituents, carbohydrate profile and pasting properties, which are partly influenced by hybrid.

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Protective effect of Pelargonium graveolens against carbon tetrachloride-induced hepatotoxicity in mice and characterization of its bioactive constituents by HPLC–PDA–ESI–MS/MS analysis

Medicinal Chemistry Research ◽

10.1007/s00044-014-1218-3 ◽

2014 ◽

Vol 24 (4) ◽

pp. 1438-1448 ◽

Cited By ~ 8

Author(s):

Eman Al-Sayed ◽

Olli Martiskainen ◽

Sayed H. Seif el-Din ◽

Abdel-Nasser A. Sabra ◽

Olfat A. Hammam ◽

...

Keyword(s):

Carbon Tetrachloride ◽

Protective Effect ◽

Bioactive Constituents ◽

Pelargonium Graveolens ◽

Esi Ms ◽

Ms Analysis

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Comparative Study on Excretive Characterization of Main Components in Herb Pair Notoginseng-Safflower and Single Herbs by LC–MS/MS

Pharmaceutics ◽

10.3390/pharmaceutics10040241 ◽

2018 ◽

Vol 10 (4) ◽

pp. 241 ◽

Cited By ~ 1

Author(s):

Ying-Yuan Lu ◽

Jin-Yang Song ◽

Yan Li ◽

Yu-Qing Meng ◽

Ming-Bo Zhao ◽

...

Keyword(s):

Oral Administration ◽

Gradient Elution ◽

Fecal Excretion ◽

Bioactive Constituents ◽

Rat Urine ◽

Liquid Chromatography Tandem Mass ◽

Main Components ◽

Cumulative Excretion

The herbal medicine combination of notoginseng-safflower has been commonly used clinically for the prevention and treatment of cardiovascular diseases. A reliable liquid chromatography-tandem mass spectrometry (LC–MS/MS) method was developed for simultaneous determination of six bioactive components (hydroxysafflor yellow A, notoginsenoide R1, ginsenoside Rb1, Re, Rd, and Rg1) in rat urine and feces after oral administration of notoginseng total saponins (NS), safflower total flavonoids (SF), and the combination of NS and SF (CNS). The chromatographic separation was achieved on a Waters HSS T3 column under gradient elution with acetonitrile and water containing formic acid as the mobile phase. The calibration curves were linear, with correlation coefficient (r) > 0.99 for six components. The intra- and interday precision (RSD) and accuracy (RE) of QC samples were within −14.9% and 14.9%, respectively. The method was successfully applied to study of the urinary and fecal excretion of six bioactive constituents following oral administration of NS, SF, and CNS in rats. Compared to the single herb, the cumulative excretion ratios of six constituents were decreased in the herbal combination. The study indicated that the combination of notoginseng and safflower could reduce the renal and fecal excretion of the major bioactive constituents and promote their absorption in rats.

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Biological effects and chemical characterization of Iris schachtii Markgr. extracts: A new source of bioactive constituents

Food and Chemical Toxicology ◽

10.1016/j.fct.2017.08.004 ◽

2018 ◽

Vol 112 ◽

pp. 448-457 ◽

Cited By ~ 17

Author(s):

Andrei Mocan ◽

Gokhan Zengin ◽

Adriano Mollica ◽

Ahmet Uysal ◽

Erdogan Gunes ◽

...

Keyword(s):

Biological Effects ◽

Chemical Characterization ◽

Bioactive Constituents

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Chemical characterization of main bioactive constituents in Paeonia ostii seed meal and GC‐MS analysis of seed oil

Journal of Food Biochemistry ◽

10.1111/jfbc.13088 ◽

2019 ◽

Vol 44 (1) ◽

Cited By ~ 2

Author(s):

Xiao Tian ◽

Sen Guo ◽

Shanshan Zhang ◽

Peisheng Li ◽

Tianyi Wang ◽

...

Keyword(s):

Seed Oil ◽

Chemical Characterization ◽

Seed Meal ◽

Bioactive Constituents ◽

Ms Analysis

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Characterization of Fatty Acids, Polysaccharides, Amino Acids, and Minerals in Marine Macroalga Chaetomorpha crassa and Evaluation of Their Potentials in Skin Cosmetics

Molecules ◽

10.3390/molecules26247515 ◽

2021 ◽

Vol 26 (24) ◽

pp. 7515

Author(s):

Haresh S. Kalasariya ◽

Nikunj B. Patel ◽

Akanksha Yadav ◽

Kahkashan Perveen ◽

Virendra Kumar Yadav ◽

...

Keyword(s):

Amino Acids ◽

Marine Macroalgae ◽

Bioactive Components ◽

Bioactive Constituents ◽

Methanolic Extracts ◽

Health Promoting ◽

Potential Benefits ◽

Beauty Care ◽

Marine Macroalga

Cosmetic industries are highly committed to finding natural sources of functional active constituents preferable to safer materials to meet consumers’ demands. Marine macroalgae have diversified bioactive constituents and possess potential benefits in beauty care products. Hence, the present study was carried out to characterize the biochemical profile of marine macroalga Chaetomorpha crassa by using different techniques for revealing its cosmetic potentials. In results, the FTIR study characterized the presence of different bioactive functional groups that are responsible for many skin-beneficial compounds whereas six and fifteen different important phycocompounds were found in GCMS analysis of ethanolic and methanolic extracts, respectively. In the saccharide profile of C. crassa, a total of eight different carbohydrate derivatives were determined by the HRLCMS Q-TOF technique, which showed wide varieties of cosmetic interest. In ICP AES analysis, Si was found to be highest whereas Cu was found to be lowest among other elements. A total of twenty-one amino acids were measured by the HRLCMS-QTOF technique, which revealed the highest amount of the amino acid, Aspartic acid (1207.45 nmol/mL) and tyrosine (106.77 nmol/mL) was found to be the lowest in amount among other amino acids. Their cosmetic potentials have been studied based on previous research studies. The incorporation of seaweed-based bioactive components in cosmetics has been extensively growing due to its skin health-promoting effects.

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Endocannabinoid Metabolome Characterization of Transitional and Mature Human Milk

Nutrients ◽

10.3390/nu10091294 ◽

2018 ◽

Vol 10 (9) ◽

pp. 1294 ◽

Cited By ~ 5

Author(s):

Adriana Gaitán ◽

JodiAnne Wood ◽

Fan Zhang ◽

Alexandros Makriyannis ◽

Carol Lammi-Keefe

Keyword(s):

Human Milk ◽

Infant Health ◽

Cannabinoid Receptor ◽

Bioactive Constituents ◽

Mature Milk ◽

Health And Development ◽

Two Stages

Recognized as the gold standard, human milk (HM) is an extremely complex yet fascinating biofluid tailored to meet an infant’s nutritional requirements throughout development. Endocannabinoids and endocannabinoid-like compounds (endocannabinoid metabolome, ECM) are endogenous lipid mediators derived from long-chain polyunsaturated fatty acids that have been identified in HM. Previous research has shown that arachidonoylglycerol might play a role in establishing the infant’s suckling response during lactation by activating the type 1 cannabinoid receptor in the infant’s brain. The mechanisms of action and the role of the ECM in HM are not fully understood. Transitional and mature milk samples were collected from lactating women (n = 24) for ECM characterization, quantification, and to evaluate differences among the two stages. HM samples were analyzed by liquid chromatography-mass spectrometry. Identified members of the ECM were: arachidonoylethanolamine, palmitoylethanolamine, oleoylethanolamine, docosahexaenoylethanolamine, eicoapentaenoylethanolamine, eicosenoylethanolamine, arachidonoylglycerol, palmitoyglycerol, oleoylglycerol, docosahexaenoylglycerol, eicosapentaenoylglycerol, eiconenooylglycerol, arachidonic acid, docosahexaenoic acid, and eicosapentaenoic acid. Only docosahexaenoylglycerol was different across transitional and mature milk (p ≤ 0.05). Data from this cohort suggest that bioactive constituents in HM may also play a role in infant health and development. Future studies can be developed based on this study’s data to help elucidate specific roles for each ECM member in addition to understanding how the ECM modulates infant health.

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