The Clinical Significance Of Circulating Mir-21, Mir-142, Mir-143, And Mir-146a In Patients With Prostate Cancer

Prostate cancer (PCa) is the most common type of solid tissue cancer among men in western countries. To enlighten the underlying molecular mechanisms in the pathophysiology of PCa and to help the development of new diagnostics and treatment models, we planned to determine the levels of circulating miR-21, miR-142, miR-143, miR-146a, and RNU 44 levels as controls for the early diagnosis of PCa. The circulating miRNA levels in peripheral blood samples from 43 localized PCa patients, 12 metastatic PCa (MET) patients, and as a control group, 42 benign prostate hyperplasia (BPH) patients with a total of 97 volunteers were determined the by PCR method. No differences in the ΔCT values were found among the groups. In PCa and PCaMet groups the expression of miR21 and miR142 were higher compared to the BHP group. No other differences were observed among the other groups. miR21 expression in the PCa group was 6.29 folds upregulated whereas in the PCaMet group 10.84 folds upregulated. When the total expression of miR142 is evaluated, it showed a positive correlation with mir21 and mir 146 (both p<0.001). Also, the expression of miR146 shows a positive correlation with both miR21 and miR143 (both p<0.001). Expression of miRNAs was found to be an independent diagnostic factor in patients with Gleason score, PSA, and free PSA levels. In conclusion, our study showed that co-expression of miR-21, miR-142, miR-143, and miR-146a and the upregulation of miR-21 resulted in increased prostate carcinoma cell growth. In the PCaMet group, miR21 is the most upregulated of all miRNAs. These miRNA markers that are expressed in different levels may provide a novel diagnostic tool to help diagnose PCa with aggressive behavior.

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The Association between Trichomonas Vaginalis Infection and The Risk of Benign Prostate Hyperplasia, Prostate Cancer, and Bladder Cancer in Patients: A Nationwide Population-Based Case-Control Study

10.21203/rs.3.rs-740634/v2 ◽

2021 ◽

Author(s):

Hung Yi Yang ◽

Ruei-Yu Su ◽

Chi-Hsiang Chung ◽

Kuo-Yang Huang ◽

Wu-Chien Chien ◽

...

Keyword(s):

Prostate Cancer ◽

Bladder Cancer ◽

Trichomonas Vaginalis ◽

Case Control Study ◽

Benign Prostate Hyperplasia ◽

Case Control ◽

Control Group ◽

Prostate Hyperplasia ◽

Benign Prostate ◽

Control Study

Abstract Introduction: Trichomonas vaginalis infection is one of the most widespread sexually transmitted infections in the world. There are approximately 276 million cases worldwide. Most men remain undiagnosed and untreated because they are asymptomatic. The chronic inflammation induced by persistent infection may increase the risk of developing genitourinary cancers. In this study, we aimed to investigate the association between trichomoniasis and benign prostate hyperplasia (BPH), prostate cancer (PCa), and bladder cancer (BC) in Taiwan.Material and method: We designed a case-control study by using the database of the National Health Insurance program in Taiwan. We used the International Classification of Diseases, 9th Revision classifications to classify all the medical conditions in the case and control groups. All odds ratios (ORs) and 95% confidence intervals (CIs) were analyzed using multivariable logistic regression to adjust for all comorbidities and variables.Result: From 2000 to 2015, we enrolled a total of 62,544 individuals as the case group and 187,632 as the control group. Trichomoniasis exposure had a significant association with BPH and PCa (adjusted OR: BPH = 2.685, 95% CI = 1.233–4.286, P = 0.013; PCa = 5.801, 95% CI = 1.296–26.035, P = 0.016). The relative risk was much higher if patients had both trichomoniasis and depression (adjusted OR = 7.682, 95% CI = 5.730–9.451, P < 0.001).Conclusion: Men with trichomoniasis had a significantly higher risk of developing BPH and PCa than those without. Healthcare professionals should not only pay more attention to disease treatment, but also to public health education.

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The Weight of HLA-DPA1 rs3077 Single Nucleotide Polymorphism in Prostate Cancer, a Multicenter Study

Prostate Cancer ◽

10.1155/2021/5539851 ◽

2021 ◽

Vol 2021 ◽

pp. 1-5

Author(s):

Mohammed Jayed Alenzi ◽

Amany A. Ghazy ◽

Diaa-Eldin Taha

Keyword(s):

Prostate Cancer ◽

Specific Antigen ◽

Serum Levels ◽

Arab Countries ◽

Control Group ◽

Single Nucleotide ◽

Genetic Transmission ◽

Free Psa ◽

Protective Allele ◽

Total Prostate Specific Antigen

Prostate cancer (PCa) has almost the highest genetic transmission that mimics an autosomal dominance hereditary pattern of cancers in some families. Its incidence in Arab countries was reported to be steadily increasing. Aim. To determine the relevance of HLA-DPA1 rs3077 (A/G) SNP with prostate cancer’s risk and/or severity. Subjects and Methods. Forty PCa patients and forty age matched patients with benign prostatic hyperplasia (BPH), as a control group, were enrolled in the study. Serum levels of urea, creatinine, total prostate-specific antigen (PSA), and free PSA were measured. PSA ratio was determined as well. Genotyping of HLA-DPA1 rs3077 (A/G) SNP was done using real-time PCR. Results. The measured lab parameters, except free PSA, were significantly higher among PCa patients in comparison to controls ( P < 0.001 ∗ ). Moreover, PSA ratio was significantly high among PCa patients ( P < 0.001 ∗ ). HLA-DPA1 rs3077 GG genotype was more frequent in PCa patients and the associated OR was 2.546 ( P = 0.059 ), while AA genotype was more frequent in the control group and the associated OR was 0.145 ( P = 0.081 ). Frequency of G allele was higher among PCa patients than the control group while A allele frequency was significantly decreased ( P = 0.034 ∗ ) (protective allele). On multivariate analysis, there is no significant correlation found between HLA-DPA1 rs3077 SNP and PSA ratio (OR = 4.5, 95% CI = 1.2–17.4, P = 0.856 ). Conclusion. HLA-DPA1 rs3077 G allele could be a risk factor for prostate cancer. However, HLA-DPA1 rs3077 SNP has no relation to PCa severity.

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The Value of P504s, 34βE12, Ki-67 and PSA in Diagnosis and Prognosis of Prostate Cancer

Nepalese Journal of Cancer ◽

10.3126/njc.v4i1.31849 ◽

2020 ◽

Vol 4 (1) ◽

pp. 88-93

Author(s):

Jin-Qi Song ◽

Ya-Nan Zhou ◽

Gang-Liang Tu ◽

Hui Xu

Keyword(s):

Prostate Cancer ◽

Gleason Score ◽

Poor Prognosis ◽

Specific Antigen ◽

Control Group ◽

Ki 67 ◽

Prostate Hyperplasia ◽

Diagnosis And Prognosis ◽

Diagnosis Rate ◽

The Difference

Background: In recent years, the incidence of prostate cancer (PCa) is increasing. Advanced PCa has a poor prognosis and high cost, which seriously affects the quality of life of patients. Therefore, how to improve the diagnosis rate and prognosis of early PCa is the focus of clinical research. This paper aims to investigate the value of P504s, 34βE12, Ki-67 and prostate-specific antigen (PSA) in the diagnosis and prognosis of PCa. Objective: To investigate the expression of P504s, 34βE12, Ki67 and PSA in prostate tissues and their clinical significance. Methods: Twenty four cases in the study group were selected from PCa confirmed by pathology in the urology department of Chengde Affiliated Hospital from October 2018 to August 2020, and 33 cases of Benign Prostate Hyperplasia (BPH) confirmed by pathology in the same period were selected as the control group. The expression of P504s, 34βE12 and Ki-67 in prostate tissues were determined by immunohistochemistry. Results: The positive expression rates of P504s, 34βE12 and TPSA or F/TPSA in PCa patients were 95.8%, 12.5% and 87.5%, respectively. The positive rates in BPH patients were 9.1%, 93.9% and 27.3%. The difference between the two groups was statistically significant (p<0.05). PCa bone or lymph node metastasis was positively correlated with Ki-67 (r=0.423, p<0.05) and Gleason score (r=0.446, p<0.05), indicating a stronger correlation with Gleasonscore. Conclusion: The combined detection of P504s, 34βE12 and PSA is helpful for the diagnosis and differential diagnosis of PCa. High Gleason score and ki-67 expression may indicate high risk of PCa metastasis and poor prognosis.

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The expression levels of microRNAs that have the androgen receptor in localized prostate cancer as a target.

Journal of Clinical Oncology ◽

10.1200/jco.2014.32.4_suppl.206 ◽

2014 ◽

Vol 32 (4_suppl) ◽

pp. 206-206

Author(s):

Katia Ramos Moreira Leite ◽

Manuel Garcia Florez ◽

Sabrina Thalita Reis ◽

Nayara Viana ◽

Betina S. Katz ◽

...

Keyword(s):

Prostate Cancer ◽

Metastatic Disease ◽

Biochemical Recurrence ◽

Protein Translation ◽

Localized Prostate Cancer ◽

Control Group ◽

Prostate Hyperplasia ◽

Control Of Gene Expression ◽

Expression Levels ◽

The Mean

206 Background: Prostate cancer (PC) depends on the androgen activity being the androgen blockage the main treatment in the setting of metastatic disease. Mechanisms of resistance in metastatic tumors are not completely known. MicroRNAs (miRNA) are small non-coding molecules that have as canonical mechanism the negative control of gene expression promoting messenger RNA (mRNA) degradation or impairing protein translation. The role of miRNAs that have as target the AR are still unknown. Our objective is to study the expression profile of miRNAs that have AR as target in localized prostate cancer. Methods: The expression levels of miRNAs that have as target AR (miR9, 34a, 34c, 185, 130a, 299, 421, 371, 541) were studied using qRT-PCR in the surgical specimens of 83 patients that underwent radical prostatectomy to treat localized prostate cancer. The expression levels of miRNAs were correlated to Gleason score (GS), pre-operatory PSA, pathological stage and biochemical recurrence (PSA>0.2 ng/mL) in a mean follow up of 45.9 months. The mean age was 62.9, mean GS was 7.2, mean PSA was 7.93 ng/mL, and 42 (50.6%) patients were staged pT2. Twenty-four (28.9%) patients presented biochemical recurrence. Specimens of six patients submitted to open surgery to treat benign prostate hyperplasia (BPH) composed the control group. Results: The mean expression of AR was 1.63, being overexpressed in 59% of the cases. The mean expression levels of the nine studied miRNAs was miR9=3.06, miR34a=0.76, miR34c=1.14, miR185=1.65, miR130a=1.9, miR299=6.23, miR421=2.84, miR371=1.41, and miR541=0.93. The univariate analysis showed that pT2 tumors have higher expression of miR371(p=0.009). Conclusions: In localized PC the higher expression of miR371 is related with organ-confined disease. This miRNA could be important in the control of AR and the comprehension of its role in PC might bring alternatives to treat metastatic disease. Funded by FAPESP – 2012/50163-0.

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Symptoms of Acute Posttraumatic Stress Disorder in Prostate Cancer Patients Following Radical Prostatectomy

American Journal of Men s Health ◽

10.1177/1557988310365168 ◽

2010 ◽

Vol 5 (1) ◽

pp. 84-89 ◽

Cited By ~ 17

Author(s):

Ioannis Anastasiou ◽

Konstantina G. Yiannopoulou ◽

Anastasios Mihalakis ◽

Nikolaos Hatziandonakis ◽

Constantinos Constantinides ◽

...

Keyword(s):

Prostate Cancer ◽

Posttraumatic Stress Disorder ◽

Radical Prostatectomy ◽

Posttraumatic Stress ◽

Stress Disorder ◽

Educational Status ◽

Control Group ◽

Ptsd Symptoms ◽

Prostate Hyperplasia ◽

Benign Prostate

Psychological morbidity is increasingly reported in cancer survivors. The authors’ objective was to determine the presence of acute posttraumatic stress disorder (PTSD) symptoms in prostate cancer (PC) patients following radical prostatectomy. Fifteen patients who underwent radical prostatectomy for localized prostate cancer were assessed for the presence of PTSD-related symptoms by completing the Davidson Trauma Scale (DTS), a month following the procedure. A group of 20 patients who underwent surgery for benign prostate hyperplasia (BPH) served as the control group. PTSD total scores were significantly higher in PC patients when compared with BPH patients, whose PTSD scores did not differ from those reported in the general population (32.6 ± 18.5 vs. 11.3 ± 9.7, p = .001) . PTSD did not vary among PC patients when adjusted for educational status. PTSD symptoms are common among patients undergoing radical prostatectomy and independent of their educational level. Research investigating these aspects of posttreatment psychological adjustment is needed for developing well-targeted psychological interventions.

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MMP-9 Overexpression Due to TIMP-1 and RECK Underexpression is Associated with Prognosis in Prostate Cancer

The International Journal of Biological Markers ◽

10.5301/jbm.2011.8831 ◽

2011 ◽

Vol 26 (4) ◽

pp. 255-261 ◽

Cited By ~ 15

Author(s):

Sabrina Thalita Reis ◽

José Pontes-Junior ◽

Alberto Azoubel Antunes ◽

Juliana Moreira De Sousa-Canavez ◽

Marcos Francisco Dall'Oglio ◽

...

Keyword(s):

Prostate Cancer ◽

Statistical Significance ◽

Specific Pattern ◽

Control Group ◽

Negative Regulators ◽

Prostate Hyperplasia ◽

Fresh Frozen ◽

Polymerase Chain ◽

Tissue Specimens

Background Extracellular matrix homeostasis is strictly maintained by a coordinated balance between the expression of metalloproteinases (MMPs) and their inhibitors. The purpose of this study was to investigate whether the expression of MMP-9 and its specific inhibitors, TIMP-1 and RECK, are expressed in a reproducible, specific pattern and if the profiles are related to prognosis and clinical outcome in prostate cancer (PC). Methods MMP-9, TIMP-1, and RECK expression levels were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) in fresh-frozen malignant tissue specimens collected from 79 patients with clinically localized PC submitted to radical prostatectomy (RP). Frozen benign prostatic tissue from another 10 men with prostate cancer, also submitted to RP, was analyzed to determine if the profile of gene expression was maintained. The control group consisted of 11 patients with benign prostate hyperplasia (BPH). Results In the tumor samples, MMP-9 was overexpressed by 9.2 times, and TIMP-1 and RECK were underexpressed (0.75 and 0.80 times, respectively). Overexpression of MMP-9 was significantly related to PSA levels above 10 ng/mL (p=0.033). In addition, MMP-9 overexpression was related to biochemical recurrence, with a marginal statistical significance (p=0.089). MMP-9 was also overexpressed in benign tissues of patients with PC, as were TIMP-1 and RECK, in contrast to their underexpression in tumor samples. Conclusion Our results show that MMP-9 is overexpressed and its negative regulators are underexpressed in PC tissue, emphasizing a possible role of MMP-9 in the carcinogenesis process. Additionally, we noticed a relationship between MMP-9 overexpression and increased levels of PSA, an important prognostic factor. In benign tissue adjacent to tumors, the MMP-9 equilibrium is likely maintained because the expression of its negative regulators is preserved.

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The Risk of Prostate Cancer Associated With Common Mutations of AR Gene in Iranian Population: a Perspective Study of Personalized Treatment

10.21203/rs.3.rs-1185476/v1 ◽

2021 ◽

Author(s):

Marzieh Rahbaran ◽

Maryam Hassani Doabsari ◽

Farshad Sharifi ◽

Mahdi Afshari ◽

Mandana Hasanzad

Keyword(s):

Prostate Cancer ◽

Iranian Population ◽

Control Group ◽

Target Area ◽

Genotype Distribution ◽

Prostate Hyperplasia ◽

Sequencing Method ◽

And Control ◽

First Time ◽

Control Study

Abstract Background: Prostate cancer (PC) is one of the most common cancers among men. Genetic predisposition is emerging as a risk factor for PC development. The Androgen receptor (AR) gene is associated with the development and prognosis of PC. Understanding the AR mutations is very important in the precision treatment of PC-resistant patients to androgen deprivation therapy. In this study, we investigate any association between common AR mutations with the risk of PC.Methods and results: In this case-control study, blood samples were collected from 121 radical prostatectomy (RP) patients who were pathologically diagnosed with PC and 120 benign prostate hyperplasia (BPH) subjects as a control group. The targeted area of the AR gene was amplified by PCR and confirmed by the Sanger sequencing method. The target area of the AR gene screened for 124 alterations in intron 7, 44 mutations in exon 8, and 52 variants in the 3'UTR region. rs113528927 DelIns AC>ACACACCAC had the most frequent mutant alleles between case and control groups, but this genotype distribution among the two recruited groups was not significant. Only one mutation, c.2644C>A, was observed in exon 8 in BPH subjects, and six alterations were detected in 3'UTR.Conclusions: For the first time in the Iranian population, AR common mutations were screened in PC patients, and our results indicate no relationship with the risk of PC, which means that other potential molecular risk factors may be engaged for PC in our population.

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Assesment of anogenital distance as a marker in diagnosis of prostate cancer

Archivio Italiano di Urologia e Andrologia ◽

10.4081/aiua.2019.3.163 ◽

2019 ◽

Vol 91 (3) ◽

Cited By ~ 2

Author(s):

Aytac Sahin ◽

Musab Ali Kutluhan ◽

Tuncay Toprak ◽

Yasin Vural ◽

Ahmet Ürkmez ◽

...

Keyword(s):

Prostate Cancer ◽

Serum Testosterone ◽

Control Group ◽

Anogenital Distance ◽

Prostate Hyperplasia ◽

Sample Number ◽

Androgen Exposure ◽

Significant Difference ◽

The Mean ◽

Prenatal Androgen

Objectives: Anogenital distance (AGD), the distance from the sexual organs to the anus, is a sexually dimorphic feature in mammals. In this study, we investigated the relationship between anogenital distance and prostate cancer (PCa). Methods: 52 patients diagnosed with PCa and 60 patients with benign prostate hyperplasia as a control group were included in the study. AGDAP (cephalad insertion of the penis to the center of the anus) and AGDAS (posterior base (first fold) of the scrotum to the center of the anus) measurements of patients were done and noted before biopsy. Results: The mean ages of 52 patients diagnosed with PCa and 60 patients with benign prostatic hyperplasia (BPH) were 67.70 ± 7.74 and 67.03 ± 7.89, respectively. There was no statistically significant difference in terms of age and serum testosterone levels of the patients diagnosed with prostate cancer or BPH (p > 0.05). Mean PSA values of patients diagnosed with prostate cancer wto be statistically higher than patients with BPH (p = 0.000). The mean AGDAP measurements of patients diagnosed with prostate cancer were statistically higher than those diagnosed with BPH (p = 0.000) and there was no significant difference in AGDAS measurements.(p = 0.823; p > 0.05). Conclusions: Androgen exposure is thought to play a role in the development PCa. Also AGD may be an indicator of prenatal androgen activity. In our study, we found a direct correlation between AGDAP and PCa. In order to reach a definitive conclusion, randomized controlled trials with larger sample number are needed.

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The Association Between Trichomonas Vaginalis Infection and the Risk of Benign Prostate Hyperplasia, Prostate Cancer, and Bladder Cancer in Patients: a Nationwide Population-based Case-control Study

10.21203/rs.3.rs-740634/v1 ◽

2021 ◽

Author(s):

Hung Yi Yang ◽

Ruei-Yu Su ◽

Chi-Hsiang Chung ◽

Hsin-Chung Lin ◽

Kuo-Yang Huang ◽

...

Keyword(s):

Prostate Cancer ◽

Bladder Cancer ◽

Trichomonas Vaginalis ◽

Case Control Study ◽

Benign Prostate Hyperplasia ◽

Case Control ◽

Control Group ◽

Prostate Hyperplasia ◽

Benign Prostate ◽

Control Study

Abstract Background: Trichomonas vaginalis infection is one of the most widespread sexually transmitted infections in the world. There are approximately 276 million cases worldwide. Most men remain undiagnosed and untreated because they are asymptomatic. The chronic inflammation induced by persistent infection may increase the risk of developing genitourinary cancers. In this study, we aimed to investigate the association between trichomoniasis and benign prostate hyperplasia (BPH), prostate cancer (PCa), and bladder cancer (BC) in Taiwan.Material and method: We designed a case-control study by using the database of the National Health Insurance program in Taiwan. We used the International Classification of Diseases, 9th Revision classifications to classify all the medical conditions in the case and control groups. All odds ratios (ORs) and 95% confidence intervals (CIs) were analyzed using multivariable logistic regression to adjust for all comorbidities and variables.Result: From 2000 to 2015, we enrolled a total of 62,544 individuals as the case group and 187,632 as the control group. Trichomoniasis exposure had a significant association with BPH and PCa (adjusted OR: BPH = 2.685, 95% CI = 1.233–4.286, P = 0.013; PCa = 5.801, 95% CI = 1.296–26.035, P = 0.016). The relative risk was much higher if patients had both trichomoniasis and depression (adjusted OR = 7.682, 95% CI = 5.730–9.451, P < 0.001).Conclusion: Men with trichomoniasis had a significantly higher risk of developing BPH and PCa than those without. Healthcare professionals should not only pay more attention to disease treatment, but also to public health education.

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Long Noncoding RNA TCONS_00027385 Acts as a miR-874-5p Sponge to Suppress the Progression of Prostate Cancer Through Regulating ASCC2 Expression

10.21203/rs.3.rs-728951/v1 ◽

2021 ◽

Author(s):

jianxin han ◽

Ning Tao ◽

Zhenlei Zhao ◽

Yanpei Gu ◽

Fan Xue ◽

...

Keyword(s):

Prostate Cancer ◽

Cell Proliferation ◽

Cell Lines ◽

Noncoding Rna ◽

Molecular Mechanisms ◽

Biological Effects ◽

Negative Relationship ◽

Luciferase Reporter ◽

Control Group

Abstract Background: A novel pyrrolo indole alkaloids, named Robustanoids A, was isolated from Coffea canephora beans, and it inhibits proliferation of prostate cancer (PCa) cells. However, the molecular mechanism linking Robustanoids A to the tumorigenesis of PCa is not yet clear. Methods: We investigated the expression of lncRNAs in PCa cells with Robustanoids A and control group by microarray analysis. The expression level of TCONS_00027385 in PCa tissues and cell lines was detected by qRT-PCR. Additionally, we conducted functional experiments to investigate the biological effects of TCONS_00027385 on the development of PCa both in vitro and in vivo. Furthermore, bioinformatic analysis, luciferase reporter experiment, RIP assay, pulldown assay, and protein chip were performed to investigate the oncogenic molecular mechanisms of TCONS_00027385.Results: In our current study, we focused on TCONS_00027385, which was up-regulated in PCa tissues and cell lines. The high expression of TCONS_00027385 was related to the progression of PCa. Function assays revealed that silencing TCONS_00027385 inhibited PCa cell proliferation and induced apoptosis, while over-expression of TCONS_00027385 remarkably played an opposite role. A deeper investigation showed that TCONS_00027385 acted as a sponge for hsa-miR-874-5p in PCa, and ASCC2 was a target of miR-874-5p in the downstream. Moreover, a positive association between TCONS_00027385 with ASCC2 and a negative relationship between miR-874-5p and TCONS_00027385 (or ASCC2) were also founded. According to the rescue assay, inhibiting ASCC2 could partially suppress the oncogenic effect on cell proliferation and apoptosis in PCa caused by the overexpression of TCONS_00027385.Conclusion: TCONS_00027385 acted as a competing endogenous RNA (ceRNA) for miR-874-5p to regulate the expression of ASCC2. TCONS_00027385 regulated the miR-874-5p/ASCC2 axis to promote PCa progression.

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