scholarly journals Use of adipose-derived stem cells in lymphatic tissue engineering and regeneration

2021 ◽  
Vol 48 (5) ◽  
pp. 559-567
Author(s):  
Antonio Jorge Forte ◽  
Daniel Boczar ◽  
Rachel Sarabia-Estrada ◽  
Maria T. Huayllani ◽  
Francisco R. Avila ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Systematic Review ◽  
Tissue Engineering ◽  
Stem Cells ◽  
Stem Cell ◽  
Growth Factor ◽  
Cochrane Central Register ◽  
Adipose Derived Stem Cells ◽  
Lymphatic Tissue ◽  
Factor C

The potential to differentiate into different cell lines, added to the easy and cost-effective method of extraction, makes adipose-derived stem cells (ADSCs) an object of interest in lymphedema treatment. Our study’s goal was to conduct a comprehensive systematic review of the use of ADSCs in lymphatic tissue engineering and regeneration. On July 23, 2019, using PubMed/MEDLINE, Cochrane Clinical Answers, Cochrane Central Register of Controlled Trials, and Embase databases, we conducted a systematic review of published literature on the use of ADSCs in lymphatic tissue engineering and regeneration. There were no language or time frame limitations, and the following search strategy was applied: ((Adipose stem cell) OR Adipose-derived stem cell)) AND ((Lymphedema) OR Breast Cancer Lymphedema). Only original research manuscripts were included. Fourteen studies fulfilled the inclusion criteria. Eleven studies were experimental (in vitro or in vivo in animals), and only three were clinical. Publications on the topic demonstrated that ADSCs promote lymphangiogenesis, and its effect could be enhanced by modulation of vascular endothelial growth factor-C, interleukin-7, prospero homeobox protein 1, and transforming growth factor-β1. Pilot clinical studies included 11 patients with breast cancer-related lymphedema, and no significant side effects were present at 12-month follow-up. Literature on the use of ADSCs in lymphatic tissue engineering and regeneration demonstrated promising data. Clinical evidence is still in its infancy, but the scientific community agrees that ADSCs can be useful in regenerative lymphangiogenesis. Data collected in this review indicate that unprecedented advances in lymphedema treatment can be anticipated in the upcoming years.

Adipose-derived stem cells enhance human breast cancer growth and cancer stem cell-like properties through adipsin

Oncogene ◽  
2018 ◽  
Vol 38 (6) ◽  
pp. 767-779 ◽  
Author(s):  
Hideaki Goto ◽  
Yohei Shimono ◽  
Yohei Funakoshi ◽  
Yoshinori Imamura ◽  
Masanori Toyoda ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Stem Cells ◽  
Stem Cell ◽  
Cancer Stem Cell ◽  
Human Breast Cancer ◽  
Cancer Growth ◽  
Adipose Derived Stem Cells ◽  
Human Breast ◽  
Breast Cancer Growth

scholarly journals Inhibition of Viability, Proliferation, Cytokines Secretion, Surface Antigen Expression, and Adipogenic and Osteogenic Differentiation of Adipose-Derived Stem Cells by Seven-Day Exposure to 0.5 T Static Magnetic Fields

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Jian Wang ◽  
Bo Xiang ◽  
Jixian Deng ◽  
Darren H. Freed ◽  
Rakesh C. Arora ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Growth Factor ◽  
Osteogenic Differentiation ◽  
Surface Antigen ◽  
Antigen Expression ◽  
Marker Genes ◽  
Dna Integrity ◽  
Adipose Derived Stem Cells ◽  
Surface Antigen Expression

After seven-day exposure to 0.5-Tesla Static Magnetic Field (SMF), Adipose-derived Stem Cells (ASCs) and those labeled by superparamagnetic iron oxide (SPIO) nanoparticles were examined for viability by methyl thiazol tetrazolium (MTT) assay, proliferation by cell counting and bromodeoxyuridine (BrdU) incorporation, DNA integrity by single cell gel electrophoresis, surface antigen by flow cytometry analysis, and the expression of cytokines and genetic markers by reverse transcription-PCR and underwent adipogenic and osteogenic differentiation assessed by quantifying related specific genes expression. The SMF slightly reduced cell viability and proliferation and inhibited the expression of CD49d, CD54, and CD73 but did not damage DNA integrity. The SMF slightly downregulated the expression of cytokines including Vascular Endothelial Growth Factor (VEGF), Insulin-like Growth Factor-1 (IGF-1), Transforming Growth Factor Beta 1 (TGF-β1), genetic markers comprising Stem Cell Antigen-1 (Sca1), Octamer-4 (Oct-4), ATP-binding Cassette Subfamily B Member 1 (ABCB1), adipogenic marker genes containing Lipoprotein Lipase (LPL), Peroxisome Proliferator-Activated Receptor Gamma (PPAR-γ), and osteogenic marker genes including Secreted Phosphor-protein 1 (SPP1) and Osterix (OSX). Exposure to 0.5 T SMF for seven days inhibited viability, proliferation, surface antigen expression, cytokine secretion, stem cell genetic marker expression, and adipogenic and osteogenic differentiation but did not affect the DNA integrity in ASCs with or without SPIO labeling.

scholarly journals Skin Tissue Engineering: Application of Adipose-Derived Stem Cells

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Agnes S. Klar ◽  
Jakub Zimoch ◽  
Thomas Biedermann
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Stem Cell ◽  
Engineering Application ◽  
Stromal Vascular Fraction ◽  
Skin Tissue ◽  
Tissue Engineering Application ◽  
Adipose Derived Stem Cells ◽  
Skin Tissue Engineering ◽  
Stem Cell Source

Perception of the adipose tissue has changed dramatically over the last few decades. Identification of adipose-derived stem cells (ASCs) ultimately transformed paradigm of this tissue from a passive energy depot into a promising stem cell source with properties of self-renewal and multipotential differentiation. As compared to bone marrow-derived stem cells (BMSCs), ASCs are more easily accessible and their isolation yields higher amount of stem cells. Therefore, the ASCs are of high interest for stem cell-based therapies and skin tissue engineering. Currently, freshly isolated stromal vascular fraction (SVF), which may be used directly without any expansion, was also assessed to be highly effective in treating skin radiation injuries, burns, or nonhealing wounds such as diabetic ulcers. In this paper, we review the characteristics of SVF and ASCs and the efficacy of their treatment for skin injuries and disorders.

scholarly journals Oncological Risk in Autologous Stem Cell Donation for Novel Tissue-Engineering Approaches to Postmastectomy Breast Regeneration

2019 ◽  
Vol 13 ◽  
pp. 117822341986489
Author(s):  
Niamh O’Halloran ◽  
Sonja Khan ◽  
Katie Gilligan ◽  
Roisin Dwyer ◽  
Michael Kerin ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Tissue Engineering ◽  
Stem Cells ◽  
Cancer Patients ◽  
Adipogenic Differentiation ◽  
Adipose Derived Stem Cells ◽  
Breast Cancer Patients ◽  
Differentiation Potential ◽  
Adipose Tissue Engineering ◽  
Different Sources

Adipose tissue engineering using adipose-derived stem cells (ADSCs) has emerged as an opportunity to develop novel approaches to postmastectomy breast reconstruction with the potential for an autologous tissue source with a natural appearance and texture. As of yet, the role of ADSCs in breast cancer development and metastasis is not completely understood; therefore, we must consider the oncological safety of employing an autologous source of ADSCs for use in breast regeneration. This study investigated the regenerative properties of ADSCs isolated from breast cancer patients, including those who had received neoadjuvant chemotherapy, and noncancer controls. The ADSCs were characterised for several parameters central to tissue regeneration, including cell viability, proliferation, differentiation potential, and cytokine secretion. A stem cell population was isolated and confirmed by flow cytometry and multilineage differentiation. There was no difference in cell phenotype or surface antigen expression between ADSCs from different sources. Adipose-derived stem cells isolated from the breast of cancer patients exhibited reduced adipogenic differentiation potential compared with ADSCs from other sources. The greatest degree of adipogenic differentiation was observed in ADSCs isolated from the subcutaneous abdominal fat of noncancer controls. The proliferation rate of ADSCs isolated from the breast of cancer patients was increased compared with other sources; however, it was decreased in ADSCs isolated from breast cancer patients who had recently been treated with neoadjuvant chemotherapy. A number of cytokines were detected in the cell conditioned media of ADSCs from different sources, including matrix metalloproteinase-2 (MMP-2), which was detected at higher levels in the secretome of ADSCs from breast cancer patients compared with noncancer controls. This study provides important information relating to the suitability of ADSCs as an autologous cell source for adipose tissue engineering in postcancer reconstruction. Results indicate that while the surface phenotype does not differ, the differentiation capacity, proliferative rate, and secreted cytokine profile are affected by the presence or treatment of breast cancer. These findings support further investigation into the regenerative potential of these ADSCs, if they are to be considered in clinical reconstructive strategies.

scholarly journals Polyurethane–polylactide-based material doped with resveratrol decreases senescence and oxidative stress of adipose-derived mesenchymal stromal stem cell (ASCs)

RSC Advances ◽  
2017 ◽  
Vol 7 (39) ◽  
pp. 24070-24084 ◽  
Author(s):  
K. Kornicka ◽  
D. Nawrocka ◽  
A. Lis-Bartos ◽  
M. Marędziak ◽  
K. Marycz
Keyword(s):  
Oxidative Stress ◽  
Tissue Engineering ◽  
Stem Cells ◽  
Stem Cell ◽  
Stress Factor ◽  
Adipose Derived Stem Cells ◽  
Stromal Stem Cell ◽  
Mesenchymal Stromal Stem Cell ◽  
And Oxidative Stress

The aim of this study was to evaluate the influence of resveratrol (RES)-doped polyurethane (TPU)–polylactide (PLA) biomaterials on the senescence and oxidative stress factor of adipose-derived stem cells (ASCs) for tissue engineering.

scholarly journals Neurospheres Induced from Human Adipose-Derived Stem Cells as a New Source of Neural Progenitor Cells

2019 ◽  
Vol 28 (1_suppl) ◽  
pp. 66S-75S ◽  
Author(s):  
Chunyang Peng ◽  
Li Lu ◽  
Yajiao Li ◽  
Jingqiong Hu
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Progenitor Cells ◽  
Neural Progenitor Cells ◽  
Neural Progenitor ◽  
Adipose Derived Stem Cells ◽  
Fibroblast Growth ◽  
Adipose Derived Stem Cell

Human adipose-derived stem cells are used in regenerative medicine for treating various diseases including osteoarthritis, degenerative arthritis, cartilage or tendon injury, etc. However, their use in neurological disorders is limited, probably due to the lack of a quick and efficient induction method of transforming these cells into neural stem or progenitor cells. In this study, we reported a highly efficient and simple method to induce adipose-derived stem cells into neural progenitor cells within 12 hours, using serum-free culture combined with a well-defined induction medium (epidermal growth factor 20 ng/ml and basic fibroblast growth factor, both at 20 ng/ml, with N2 and B27 supplements). These adipose-derived stem cell-derived neural progenitor cells grow as neurospheres, can self-renew to form secondary neurospheres, and can be induced to become neurons and glial cells. Real-time polymerase chain reaction showed significantly upregulated expression of neurogenic genes Sox2 and Nestin with a moderate increase in stemness gene expression. Raybio human growth factor analysis showed a significantly upregulated expression of multiple neurogenic and angiogenic cytokines such as brain-derived neurotrophic factor, glial cell line-derived neurotrophic growth factor, nerve growth factor, basic fibroblast growth factor and vascular endothelial growth factor etc. Therefore, adipose-derived stem cell-derived neurospheres can be a new source of neural progenitor cells and hold great potential for future cell replacement therapy for treatment of various refractory neurological diseases.

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