scholarly journals INVESTIGATING THE EFFECT OF SUBSTRATE, MYCORRHIZAL APPLICATION AND BULB SEPARATION ON THE GROWTH OF THE WILD ORCHID ANACAMPTIS PYRAMIDALIS

AGROFOR ◽  
2019 ◽  
Vol 3 (3) ◽  
Author(s):  
Elie KHOURY ◽  
Tony Kevork SAJYAN ◽  
Peter ZHELEV ◽  
Youssef Najib SASSINE

Pyramidal orchid (Anacamptis pyramidalis) is a wild terrestrial orchid widelyfound in Lebanon and the Mediterranean zone. Random collection and trade of theorchid for medicinal and edible use (salep) subjected it to a risk of extinction.Consequently, the current work aimed to propagate this orchid in vivo. Orchidbulbs were transplanted from the wild into four different soil substrates (Pinebark,Pinebark-Peat (1/1), Peat-Sand (1/1) and control: soil from the collection site)under controlled conditions of temperature and humidity. The effect of mycorrhizalapplication (MY: Yes) was tested in the different substrates except in control andorchid bulbs (son bulb and mother bulb) were planted combined (SB+MB) or afterseparation of the mother bulb (SB). Same treatments were repeated over twoconsecutive years. Results showed an earlier emergence of son bulbs (SB) grownin control substrate. Moreover, there was a significant difference in plant growthwith superiority for (SB) compared to (SB+MB) regarding plant length andelongation of first leaf except bulb dimensions (length and width) that were higherin (SB+MB). Mycorrhizal application enhanced the overall growth of plants and itseffect was the most obvious in the substrate Peat-Sand (1/1). In general, the bestgrowth of the orchid was observed at the level (SB)/(MY:Yes)/Peat-Sand (1/1).Results of the second experimental year confirmed those of the first year with animprovement of the rate of emergence by 13%. The in vivo propagation methodwas beneficial on improving the growth of A. pyramidalis ex situ and it could beadopted as an initiative for wild orchid conservation.

2020 ◽  
Vol 14 (6) ◽  
pp. 2219-2229
Author(s):  
A. Kindozandji ◽  
B.D. Datinon ◽  
K. Amevoin ◽  
A.N. Nondichao ◽  
A.I. Glitho ◽  
...  

Le niébé est l’une des légumineuses alimentaires la plus importante dans toutes les régions de l’Afrique Sub-saharienne. L’objectif de ce travail était d’étudier l’effet rémanent de Mavi Multinucléo polyhedrovirus (Mavi MNPV) après la récolte du niébé au champ. Le champ a été traité 5 fois au biopesticide Mavi MNPV à la fréquence de sept jours. Deux semaines après la récolte, deux plants ainsi que les graines de chaque traitement sur chaque unité parcellaire ayant reçu les traitements de Mavi MNPV de 107, 108 et 109 virions/ml ont été collectés et amenés au laboratoire avec le substrat de développement de la plante (sable). Aucune des formulations issues des substrats sable, racines, feuilles, tiges et graines n’a affecté la mortalité des larves L2 et L3 de Maruca vitrata et aucune différence significative n’a été observée entre les traitements et le témoin. Quels que soient les traitements, le taux d’émergence des adultes est élevé ; ce qui signifie que la variation de la concentration de Mavi MNPV ne conditionne pas sa présence dans le sol, les tiges, les feuilles, les racines et les graines. Le biopesticide Mavi MNPV n’est pas persistant dans la nature.Mots clés : Niébé, Mavi MNPV, Maruca vitrata, Effet rémanent   English Title: Study of the residual effect of Mavi MNPV in a cowpea field Cowpea is one of the most important food legumes in all regions of Sub-Saharan Africa. The objective of this research work was to study the persistence effect of Mavi Multinucleus polyhedrovirus (Mavi MNPV) after field harvest of the cowpea. The field was treated 5 times with Mavi MNPV biopesticide at seven days. Two weeks after harvest, two plants as well as the seeds from each treatment on each plot unit that received Mavi MNPV treatments of 107, 108 and 109 virions / ml were removed and brought to the laboratory with the plant development substrate (sand). None of the formulations from sand, root, leaf, stem and seed substrates did not affect the mortality of L2 and L3 larvae of Maruca vitrata and no significant difference was observed between treatments and control. Regardless of the treatments, the rate of emergence of adults is high; this means that the variation in the concentration of Mavi MNPV does not condition its presence in soil, stems, leaves, roots and seeds. Mavi MNPV biopesticide is not persistent in nature.Keywords: Cowpea, Mavi MNPV, Maruca vitrata, Residual effect.      


2016 ◽  
Vol 1 (2) ◽  
pp. 159
Author(s):  
Hanik Nuzulimah

This paper attempts to explore whether there is significant difference of achievement in vocabulary between students taught by using pictures and those are not. The subject of this study is the first year students of SLTP Muhammadiyah Simo Susukan, Semarang where the sample contains 50 students that is divided into experimental group (with treatment) and control group (without treatment). The data is gathered from participants’ score obtained from pre test and post test. Using t test as technique of data analysis, result shows that students taught by using pictures perform better than those are not. It means that there is significant difference between the two groups. Keywords: Vocabulary Teaching; Picture


1983 ◽  
Vol 103 (3) ◽  
pp. 293-301 ◽  
Author(s):  
Michael Warnhoff ◽  
Gunter Dorsch ◽  
Karl M. Pirke

Abstract. A perfusion system was developed in which isolated median eminences (ME) were stimulated in vitro by depolarizing agents such as potassium and veratridine. Potassium concentrations between 30 and 80 mm released increasing amounts of luteinizing hormone-releasing hormone (LRH) from the MEs of starved and control rats. Veratridine at a concentration of 50 μm caused a more prolonged LRH release in both starved and control animals. LRH secretion in vitro was slightly, though not under all conditions, significantly greater in rats starved for 5 days. The testosterone (T)-LH feedback was studied by castrating the animals and substituting various doses of T through implantation of T-releasing capsules of different sizes. The concentration in plasma, which can prevent the castration-induced much smaller in starved than in control rats. The in vitro release of LRH evoked by 80 mm potassium was not different for starved and fed rats under various feedback conditions. Both groups revealed decreased in vitro release of LRH when castrated animals were not substituted with T. The effect of castration was studied from 1 to 28 days. The plasma LH values rapidly increased in starved and control animals, indicating that the hypothalamic responsestration is not delayed by starvation. The release in vitro of LRH decreased from the first to the fifth day and remained constant thereafter. No significant difference between starved and fed rats was observed. The experiments indicate that the 'releasable pool' of LRH in vitro is greater under conditions of reduced LH release in vivo. The basic mechanism of depolarization-induced exocytosis of LRH from the ME is intact in starved animals.


2000 ◽  
Vol 132 (3) ◽  
pp. 373-386 ◽  
Author(s):  
David B. Orr ◽  
Charles P-C. Suh ◽  
Kenneth W. Mccravy ◽  
C. Wayne Berisford ◽  
Gary L. Debarr

AbstractInundative releases of Trichogramma exiguum Pinto and Platner were evaluated for suppression of the Nantucket pine tip moth, Rhyacionia frustrana (Comstock), in first-year loblolly pine, Pinus taeda L., plantations. Three releases, spaced 7 d apart, were made in three 0.4-ha plots during second-generation R. frustrana egg deposition. Each release included three cohorts of T. exiguum developmentally separated by 25 degree-days. Mean ± SD field release rate for each cohort was 328 238 ± 88 379 females/ha. Mean T. exiguum emergence under laboratory conditions for released cohorts was 96 ± 2%, with 74 ± 3% females, of which 1 ± 1% of females displayed brachyptery; female longevity was 18 ± 3 d. Field emergence averaged 96 ± 4%. Parasitism of R. frustrana eggs was significantly increased, ranging from 40 ± 19 to 73 ± 22% in T. exiguum-treated plots and 17 ± 17 to 67 ± 21% in control plots. Data from all treated plots combined showed R. frustrana egg survival (hatching) was significantly reduced by 46%, and larval populations were significantly reduced by 60%. There was no significant difference in the percentage of terminals damaged between T. exiguum-treated (31 ± 16%) and control plots (45 ± 10%); however, length of terminal damage was significantly lower in treated plots. The percentage of damage to top whorl shoots was significantly lower in T. exiguum-treated plots compared with control plots, but there was no significant difference in length of tunneling damage. Damage to remaining shoots was not significantly different between T. exiguum-treated and control plots. Microhabitat significantly influenced both mean maximum and minimum temperature and the number of consecutive hours per day that were at or above 35 °C (critical temperature for T. exiguum survival). Soil surface with no cover had the greatest number of hours at or above 35 °C, followed by soil surface with herbaceous cover, and canopies of small trees (0.4 m tall). Canopy habitats in larger trees (0.9–1.8 m tall) had the most moderate temperature conditions. Parasitoid emergence was significantly reduced in response to increasing number of consecutive hours at or above 35 °C. Predation of parasitoids prior to emergence was significantly affected by microhabitat and by the length of time capsules were in the field before T. exiguum emergence (i.e., cohort number).


2021 ◽  
Vol 7 (1) ◽  
pp. 5-9
Author(s):  
Gyem Dorji ◽  
Kanokwan Wetasin ◽  
Kuenzang Chhezom ◽  
Nargis Sultana ◽  
Minjur Dorji

Introduction: Neuroanatomy is considered as difficult subject as it consists mostly of complicated microscopic structures. However, understanding of the subject structure is very important for nursing students to know its functions and dysfunctions for enhancing nursing practices. To date, teaching neuroanatomy relies heavily on illustrations and the most common way is by using PowerPoint slides, but teachers are often faced with dilemma on how to use illustrations effectively. Therefore, this study was conducted to assess the effectiveness of different ways of presenting neuroanatomy illustrations in PowerPoint slides on nursing students’ short[1]term learning memory. Methods: The quasi-experimental study was conducted at Faculty of Nursing and Public Health, Khesar Gyalpo University of Medical Sciences of Bhutan between June 2020 and November 2020 by comparing an Experimental (n= 70) and a Control group (n= 69) of first year nursing students for their ability to answer questions based on those seven-way slides. Independent sample t-test was used for statistical analysis. Results: Significant difference was found at 95% CI between the performance of the Experimental and Control groups regarding Way 1 (Sequencing of labels) and Way 6 (Magnifying from the original structure) of presentation (tWay 1(135.99) = 2.695, p= .008, d = .458, 95% CI = [0.059, .385]; tWay 6(135.61) = 2.351, p= .020, d = .399, 95% CI = .031, .355], respectively). Conclusions: The use of different ways like sequencing the labels and magnifying from the original structure of presenting illustration in PowerPoint slides are found to be helpful in increasing short[1]term memory in students.


2018 ◽  
Vol 12 ◽  
pp. 56-62
Author(s):  
PRAGYA SINGH LODHI ◽  
DEEPAK SINGH ◽  
GUNJAN SHARMA

This study has been conducted to study the impact of diary writing on mental health of adolescent girls. A sample of 100 girlsfrom Shri Ram Collage Muzaffernagar (U.P.), who are studying in first year of graduation, was selected from accidental sampling method.Sample was divided into two groups, 50 in experimental group and 50 in control group. Diary writing was selected as the independent variable, which was appliedfor 3 months where girls used to write down their thoughts and feelings; independently 30 minutes each for 5 days a week. Mental health of girls was assessed using the ‘Mental Health Inventory’. Statistical analysis of the data was performed using the SPSS software (version 18) the result indicated that there was a significant difference between experimental and control group on the level of mental health of adolescent girls. The findings of this study indicate that the practice of diary writing significantly improved the level of mental health of adolescent girls.


2020 ◽  
Vol 25 ◽  
pp. 2515690X2092053 ◽  
Author(s):  
Zemene Demelash Kifle ◽  
Getnet Mequanint Adinew ◽  
Mestayet Geta Mengistie ◽  
Abyot Endale Gurmu ◽  
Engidaw Fentahun Enyew ◽  
...  

Background. The management and control of malaria has become gradually challenging due to the spread of drug-resistant parasites, lack of effective vaccine, and the resistance of vector to insecticides. Consequently, novel agents are urgently needed from different sources including from medicinal plants. In Ethiopia and Uganda, Myrica salicifolia root is traditionally claimed for the treatment of malaria. The aim of this study was to evaluate the in vivo antimalarial activity of root crude extract of M salicifolia. Methods. The parasite, Plasmodium berghei was used in this study since it is an appropriate parasite that is most commonly used because of its higher accessibility. A 4-day suppressive test was employed to evaluate the antimalarial effect of crude extract against early infection. The curative and prophylactic effect of the crude extract was further tested by Rane’s test and residual infection procedure. Parasitemia, survival time, packed cell volume, body weight, and rectal temperature of mice were used as evaluation parameters. Windows SPSS version 24 was used to analyze the data and analysis of variance followed by Tukey’s honestly significant difference to compare results between groups. Results. The root crude extract of M salicifolia significantly ( P < .05-.0001) suppressed parasitemia. The crude extract exhibited a chemosuppression of 40.90. Conclusion. The development of new antimalarial agents and the finding supports the traditional claims and previous in vitro studies.


2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Favour O. Omeiza ◽  
George O. Ademowo ◽  
Funmilola A. Ayeni

Abstract Background The menace of resistance to anti-malarial drugs is a great challenge to malaria control, necessitating the search for new anti-malarial agents. This search has led to the exploration of natural products for efficacy in malaria therapy. Omidun is the supernatant of fermenting maize (ogi) slurry that has been widely investigated and reported to possess several health benefits and it is used traditionally as solvent for preparing anti-malarial herbs. However, there is no information on the anti-malarial activity of omidun itself. This study was conducted to investigate the prophylactic, curative and suppressive anti-malarial potential of omidun. Methods Experimental mice in the curative group were infected with 1 × 106 cells of Plasmodium berghei strain ANKA and treated with either 0.2 ml of omidun containing 3 × 109 cfu/ml of viable lactic acid bacteria or 0.2 ml of 5 mg/kg of chloroquine (positive control) or 0.2 ml of saline (negative control) for 4 days from day 3 post infection. The prophylactic group of mice were pre-treated with either omidun, chloroquine or saline for 4 days before infection with P. berghei, while the suppressive group was treated with omidun or chloroquine or saline and infected with P. berghei simultaneously. A group of mice were uninfected but treated (with omidun and control samples), while a final group was uninfected and untreated (controls). Parasitaemia and histopathology analysis were done in all groups. Results The curative and suppressive groups showed a significant difference between the omidun-treated mice (100% parasitaemia reduction) and the untreated mice (54.5% parasitaemia increase). There was no significance difference between the omidun treatment and chloroquine (positive control) treatment in suppressive group as both treatment had 100% parasitaemia reduction. The omidun prophylactic treatment however did not show any parasitaemia suppression, but a significant difference was observed between the omidun treatment (85% increase) and the chloroquine (positive control) treatment (100% reduction) in the group. Omidun treatment is non-toxic to the kidney. Conclusion This study provides scientific evidence supporting omidun usage in the treatment of malaria. Consequently, further work may yield the specific component of omidun responsible for the anti-malarial activity.


2006 ◽  
Vol 27 (3) ◽  
pp. 983-992 ◽  
Author(s):  
Simone Meuter ◽  
Patrick Schaerli ◽  
Regula Stuber Roos ◽  
Oliver Brandau ◽  
Michael R. Bösl ◽  
...  

ABSTRACT Dendritic cells (DCs) have long been recognized as key regulators of immune responses. However, the process of their recruitment to peripheral tissues and turnover during homeostasis remains largely unknown. The chemokine CXCL14 (BRAK) is constitutively expressed in skin and other epithelial tissues. Recently, the human chemokine was proposed to play a role in the homeostatic recruitment of macrophage and/or DC precursors toward the periphery, such as skin. Although so far no physiological function could be demonstrated for the murine CXCL14, it shows a remarkable homology to the human chemokine. In order to elucidate the in vivo role of CXCL14, we generated a mouse defective for this chemokine. We studied various components of the immune system with emphasis on monocytes/macrophages and DC/Langerhans cell (LC) populations in different tissues during steady state but did not find a significant difference between knockout (CXCL14 − / −) and control mice. Functionally, LCs were able to become activated, to migrate out of skin, and to elicit a delayed type of hypersensitivity reaction. Overall, our data indicate that murine CXCL14 is dispensable for the homeostatic recruitment of antigen-presenting cells toward the periphery and for LC functionality.


2012 ◽  
Vol 1 (2) ◽  
pp. 57-60
Author(s):  
Gerry Uswak ◽  
Jinhwa Lee ◽  
Jennife Li

ABSTRACT Aim The aim of this study was to determine the efficacy of a new, light-activated toothbrush, (Soladey-J3X) incorporated with a TiO2 semiconductor and a solar panel (test) with a similar toothbrush but without the active semiconductor and solar panel (control). Materials and methods Forty-nine adults aged 19 to 34 years completed a double-blind, cross-over study with each participant using the test and control brush for a period of 3 weeks each. The mean differences between baseline plaque and gingivitis scores and, after the subjects used, the test and control brushes were analyzed by the paired t-test. Results The test and control brush demonstrated a reduction in overall plaque scores, but the difference between the two brushes was not statistically significant. The Soladey-J3X, however, showed a statistically significant difference in the overall mean reduction in gingivitis and on the buccal and interproximal surfaces compared to the control. Conclusion Both the test and the control toothbrushes induced a reduction in plaque scores after 3 weeks of use but the differences between the two type of brushes were not statistically significant. However, the test brush was more efficacious than the control in reducing overall gingival index scores and on the buccal and interproximal surfaces. Additional in vivo and longer term clinical trials are, however, warranted to fully investigate the mechanism and efficacy of TiO2 semiconductor toothbrushes on plaque biofilm and gingival inflammation. How to cite this article Uswak G, Hoover J, Lee J, Li J, Karunanayake C. A Double-blind Cross-over Study evaluating the Efficacy of a Light-activated Toothbrush. Int J Experiment Dent Sci 2012;1(2):57-60.


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