scholarly journals Effects of three commonly used diuretics on the urinary proteome

2013 ◽  
Author(s):  
Xundou Li ◽  
Mindi Zhao ◽  
Menglin Li ◽  
Lulu Jia ◽  
Youhe Gao
Keyword(s):  
The Body ◽  
P Value ◽  
Intragastric Administration ◽  
Protein Biomarkers ◽  
Healthy Human ◽  
Disease Biomarkers ◽  
Urinary Proteome ◽  
Human Orthologs ◽  
Before And After ◽  
Therapeutic Doses

Biomarker is the measurable change associated with a physiological or pathophysiological process. Unlike blood which has mechanisms to keep the internal environment homeostatic, urine is more likely to reflect changes of the body. In other words, urine is likely to be a better biomarker source than blood. However, the urinary proteome are affected by many factors. In this study, the effects of three commonly used diuretics (furosemide, hydrochlorothiazide and spirolactone ) on the urinary proteome were analyzed in rats. Urine samples were collected before and after the intragastric administration of diuretics at therapeutic doses and analyzed using LC-MS/MS. Based on quantification by Progenesis LC-MS software, there are 7, 5 and 2 proteins with the p value ≤0.05, a fold change ≥2, a spectral count ≥5 and FDR ≤1%, respectively. Most their human orthologs were considered to be stable in the healthy human urinary proteome. 10 of the 14 proteins have been reported as disease biomarkers in previous studies. So the effects of diuretics should be given more attention in future urinary protein biomarkers studies. The effects of diuretics on urinary proteome are different which can provide clues to elucidate the mechanisms of the diuretics.

scholarly journals Effects of three commonly used diuretics on the urinary proteome

2013 ◽  
Author(s):  
Xundou Li ◽  
Mindi Zhao ◽  
Menglin Li ◽  
Lulu Jia ◽  
Youhe Gao
Keyword(s):  
The Body ◽  
P Value ◽  
Intragastric Administration ◽  
Protein Biomarkers ◽  
Healthy Human ◽  
Disease Biomarkers ◽  
Urinary Proteome ◽  
Human Orthologs ◽  
Before And After ◽  
Therapeutic Doses

Biomarker is the measurable change associated with a physiological or pathophysiological process. Unlike blood which has mechanisms to keep the internal environment homeostatic, urine is more likely to reflect changes of the body. In other words, urine is likely to be a better biomarker source than blood. However, the urinary proteome are affected by many factors. In this study, the effects of three commonly used diuretics (furosemide, hydrochlorothiazide and spirolactone ) on the urinary proteome were analyzed in rats. Urine samples were collected before and after the intragastric administration of diuretics at therapeutic doses and analyzed using LC-MS/MS. Based on quantification by Progenesis LC-MS software, there are 7, 5 and 2 proteins with the p value ≤0.05, a fold change ≥2, a spectral count ≥5 and FDR ≤1%, respectively. Most their human orthologs were considered to be stable in the healthy human urinary proteome. 10 of the 14 proteins have been reported as disease biomarkers in previous studies. So the effects of diuretics should be given more attention in future urinary protein biomarkers studies. The effects of diuretics on urinary proteome are different which can provide clues to elucidate the mechanisms of the diuretics.

scholarly journals Potential urinary aging markers of 20-month-old rats

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2058 ◽  
Author(s):  
Xundou Li ◽  
Youhe Gao
Keyword(s):  
Biomarker Discovery ◽  
The Body ◽  
Disease Biomarkers ◽  
Urinary Proteome ◽  
Human Orthologs ◽  
Human Protein Atlas ◽  
Aging Conditions ◽  
Old Rats ◽  
Spectral Counts ◽  
Altered Proteins

Urine is a very good source for biomarker discovery because it accumulates changes in the body. However, a major challenge in urinary biomarker discovery is the fact that the urinary proteome is influenced by various elements. To circumvent these problems, simpler systems, such as animal models, can be used to establish associations between physiological or pathological conditions and alterations in the urinary proteome. In this study, the urinary proteomes of young (two months old) and old rats (20 months old; nine in each group) were analyzed using LC-MS/MS and quantified using the Progenesis LC-MS software. A total of 371 proteins were identified, 194 of which were shared between the young and old rats. Based on criteria of a fold change ≥2,P< 0.05 and identification in each rat of the high-abundance group, 33 proteins were found to be changed (15 increased and 18 decreased in old rats). By adding a more stringent standard (protein spectral counts from every rat in the higher group greater than those in the lower group), eight proteins showed consistent changes in all rats of the groups; two of these proteins are also altered in the urinary proteome of aging humans. However, no shared proteins between our results and the previous aging plasma proteome were identified. Twenty of the 33 (60%) altered proteins have been reported to be disease biomarkers, suggesting that aging may share similar urinary changes with some diseases. The 33 proteins corresponded to 28 human orthologs which, according to the Human Protein Atlas, are strongly expressed in the kidney, intestine, cerebellum and lung. Therefore, the urinary proteome may reflect aging conditions in these organs.

scholarly journals Pengaruh Aromaterapi Lavender secara Masase terhadap Tekanan Darah dan Tingkat Stres Mahasiswa Jurusan Keperawatan Poltekkes Palangka Raya

2020 ◽  
Vol 5 (2) ◽  
pp. 100-107
Author(s):  
Reny Sulistyowati ◽  
Syam�ani Syam�ani
Keyword(s):  
Blood Pressure ◽  
Univariate Analysis ◽  
T Test ◽  
The Body ◽  
Bivariate Analysis ◽  
Control Group ◽  
P Value ◽  
Stress Levels ◽  
Before And After ◽  
Distress Scale

Stress is an individual's adaptive response to various external pressures or demands and produces a variety of disorders including physical, emotional and behavioral disorders. Final year students often experience feelings of stress so that it can interfere with concentration to attend lectures and can even hamper completing education in a timely manner. There are several ways of managing stress, one of which is using aromatherapy. Aromatherapy is a non-pharmacological therapy, which is a type of therapy that applies direct body contact and has a therapeutic effect that combines physiological effects, caused by massage on the body, with psychological effects, which are derived from essential oils. The general purpose of the study is to analyze the effect of aromatherapy on blood pressure and stress levels by using the Kessler Psychological Distress Scale in final semester students. The research method used a quasi-experimental design with a pretest-posttest control group design approach, in 28 intervention groups, namely respondents who were given lavender aromatherapy massage and 28 respondents in the control group. The univariate analysis was conducted to show frequency distribution and bivariate analysis was conducted by independent sample T-test and dependent sample T-test. The results showed that there were significant differences in systolic blood pressure before and after the intervention in the intervention and the control group with a p-value of 0.01 < 0.05. Whereas the stress level variable found that there is no difference in stress levels in the intervention and the control group before and after the intervention with p-value 0.169> 0.05.

scholarly journals Application Model of Pranic Healing Therapy for Emotional Stress Using Accurate Bio-Well GDV Camera

2019 ◽  
Vol 125 ◽  
pp. 05005
Author(s):  
Cahyaning Puji Astuti ◽  
Melyana Nurul Widyawati ◽  
Suryono Suryono
Keyword(s):  
Emotional Stress ◽  
Controlled Trial ◽  
Pharmacological Therapy ◽  
The Body ◽  
Control Group ◽  
P Value ◽  
Test Results ◽  
Emotional Instability ◽  
Before And After ◽  
And Control

Pregnancy is a period of both hormonal and non-hormonal overall change in the body. Emotional instability is commonly experienced by women during this period. However, this somewhat complex issue is often not addressed optimally. Pharmacological therapies using CPZ and lithium are often administered, but they have effects for the body. Therefore, there is a need to administer non-pharmacological therapy that has minimum side effects. This research aims to increase the healthy energy within the body using Pranic Healing, which is measured by the Bio-Well GDV camera. This research employed the pretest-posttest method on two groups (treatment and control) using the Randomized Controlled Trial design. Pranic Healing therapy was performed for 30 minutes once a week for 3 weeks, while GDV measurement was performed once a week for 4 weeks. Paired t-test results for Pranic Healing on emotional pressure showed p-value of 0.0001 (<0.05) for posttest2 and p-value of 0.005 (<0.05) for posttest3. Meanwhile, the control group did not indicate any changes in emotional stress both before and after treatment. It is found that Pranic Healing as measured using a bio-well GDV camera is capable of improving health energy within the body by relieving emotional stress experienced in trimester III primigravida.

scholarly journals Integrative responses of neurons in nucleus tractus solitarius to visceral afferent stimulation and vestibular stimulation in vertical planes

2011 ◽  
Vol 301 (5) ◽  
pp. R1380-R1390 ◽  
Author(s):  
Yoichiro Sugiyama ◽  
Takeshi Suzuki ◽  
Vincent J. DeStefino ◽  
Bill J. Yates
Keyword(s):  
Copper Sulfate ◽  
Nucleus Tractus Solitarius ◽  
Vertical Plane ◽  
Vestibular Nuclei ◽  
Vestibular Stimulation ◽  
The Body ◽  
Head Movements ◽  
Intragastric Administration ◽  
Before And After ◽  
Triggering Mechanisms

Anatomical studies have demonstrated that the vestibular nuclei project to nucleus tractus solitarius (NTS), but little is known about the effects of vestibular inputs on NTS neuronal activity. Furthermore, lesions of NTS abolish vomiting elicited by a variety of different triggering mechanisms, including vestibular stimulation, suggesting that emetic inputs may converge on the same NTS neurons. As such, an emetic stimulus that activates gastrointestinal (GI) receptors could alter the responses of NTS neurons to vestibular inputs. In the present study, we examined in decerebrate cats the responses of NTS neurons to rotations of the body in vertical planes before and after the intragastric administration of the emetic compound copper sulfate. The activity of more than one-third of NTS neurons was modulated by vertical vestibular stimulation, with most of the responsive cells having their firing rate altered by rotations in the head-up or head-down directions. These responses were aligned with head position in space, as opposed to the velocity of head movements. The activity of NTS neurons with baroreceptor, pulmonary, and GI inputs could be modulated by vertical plane rotations. However, injection of copper sulfate into the stomach did not alter the responses to vestibular stimulation of NTS neurons that received GI inputs, suggesting that the stimuli did not have additive effects. These findings show that the detection and processing of visceral inputs by NTS neurons can be altered in accordance with the direction of ongoing movements.

scholarly journals Efektifitas Spiritual Emotional Freedom Technique (SEFT) dan menyanyi terhadap tekanan darah lansia di rumpelsos pucang gading semarang

2019 ◽  
Vol 3 (1) ◽  
pp. 14
Author(s):  
Sonhaji Sonhaji ◽  
Roland Lekatompessy
Keyword(s):  
Blood Pressure ◽  
Social Service ◽  
The Elderly ◽  
The Body ◽  
P Value ◽  
Test Results ◽  
Body System ◽  
Left Group ◽  
Average Blood Pressure ◽  
Before And After

The result of statistical analysis obtained data on the average blood pressure inthe left group before being given the intervention of 173,76/96,41 while after being given the intervention of 170,29/93,82. On the singing group before being given intervention, the average of blood pressure of 153,06/87,41 and after being given intervention tobe 168/87. Camparative test results in both groups between before and after ihe invention were obtained p – value = 0,00 (p- value p < 0,05). Conclusion was spiritual emotional freedom techneque (SEFT) is more effective than singing to reduce the blood pressurenof the elderly with hypertension in social service home, Pucang Gading, Semarang. One of the body system disorders that is often experienced by the elderly. Namely the cardiovascular system such as hypertension. Hypertension of elderly can caused bad condition such as heartdiscase, stroke and kidney failure, there fore, intervention is needed to maintain blood pressure within normal himits.

scholarly journals TERAPI ALTERNATIF PENGURANGAN RASA NYERI DAN KECEMASAN MELALUI SLOW STROKE BACK MASSAGE (SSBM) PADA INPARTU KALA I FASE AKTIF

2020 ◽  
Vol 2 (2) ◽  
pp. 211-218
Author(s):  
Diah Evawanna Anuhgera ◽  
Wilda Wahyuni Siregar ◽  
Nikmah Jalilah Ritonga ◽  
Desikawali Pardede
Keyword(s):  
Rating Scale ◽  
Structured Interview ◽  
The Body ◽  
Labor Pain ◽  
Control Group ◽  
P Value ◽  
Before And After ◽  
Quasi Experimental ◽  
First Stage Of Labor ◽  
Back Massage

Abstract Labor is always synonymous with the experience of pain and fear felt by mother. SSBM stimulates the body release endorphins which are having natural pain killing propoerties and reducing anxiety levels. This study aimed to determine the effect of slow stroke back massage (SSBM) on reduction of labor pain and anxiety during first stage of labor. A quasi-experimental study with purposive non-probability sampling techniwue is used for the people. Study was conducted on 36 samples (18 in experimental group anda 18 in control group). A structured interview schedule, albor assesment performa, numerical pain rating scale and DASS(Depresssion, Anxiety and Stress Scales) were used for data collection and the data were analyzed by paired and unpaired t test. There were statistically significant differences of anxiety and pain levels before and after intervention in the experiment and control group with p value 0.00.Result confirmed that SSBM is significantly effective in reducing labor pain and anxiety level during first stage of labor. Abstract Labor is always synonymous with the experience of pain and fear felt by mother. SSBM stimulates the body release endorphins which are having natural pain killing propoerties and reducing anxiety levels. This study aimed to determine the effect of slow stroke back massage (SSBM) on reduction of labor pain and anxiety during first stage of labor. A quasi-experimental study with purposive non-probability sampling techniwue is used for the people. Study was conducted on 36 samples (18 in experimental group anda 18 in control group). A structured interview schedule, albor assesment performa, numerical pain rating scale and DASS(Depresssion, Anxiety and Stress Scales) were used for data collection and the data were analyzed by paired and unpaired t test. There were statistically significant differences of anxiety and pain levels before and after intervention in the experiment and control group with p value 0.00.Result confirmed that SSBM is significantly effective in reducing labor pain and anxiety level during first stage of labor.

scholarly journals The potential urinary aging markers of 20-month-old rats

2015 ◽  
Author(s):  
Youhe Gao ◽  
Xundou Li
Keyword(s):  
Biomarker Discovery ◽  
Disease Biomarkers ◽  
Urinary Proteome ◽  
Pathological Conditions ◽  
Human Orthologs ◽  
Human Protein Atlas ◽  
Aging Conditions ◽  
Old Rats ◽  
Spectral Counts ◽  
Standard Protein

Urine is a very good source for biomarker discovery because it accumulates the changes of body. The urinary proteome is influenced by various factors, which is a major challenge in urinary biomarker discovery. To circumvent these problems, simpler systems, such as animal models, should be used to establish associations between physiological or pathological conditions and changes in the urinary proteome. In this study, the urinary proteome of young (2-month-old) and old rats (20-month-old; 9 in each group) were analyzed using LC-MS/MS and quantified using the Progenesis LC-MS software. A total of 371 proteins were identified, 194 of which were shared between young and old rats. Based on the criteria of a fold change ≥ 2, P < 0.05 and being identified in each rat in the high abundance group, 33 proteins were changed (15 up-regulated and 18 down-regulated in old rats). By adding a more stringent standard (protein spectral counts from every rat in the higher group greater than those in the lower group), 8 proteins were changed consistently in all rats of between the groups, 2 of which are also altered in the urinary proteome of aging humans. There are no shared proteins between our results and the previous aging plasma proteome. Twenty of the 33 (60 %) changed proteins have been reported to be disease biomarkers, which implies that aging may share similar urinary changes with some diseases. The 33 proteins corresponded to 28 human orthologs, which are strongly expressed in the kidney, intestine, cerebellum and lung, according to the human protein ATLAS. Therefore, the urinary proteome may reflect aging conditions in these organs.

scholarly journals The potential urinary aging markers of 20-month-old rats

2015 ◽  
Author(s):  
Youhe Gao ◽  
Xundou Li
Keyword(s):  
Biomarker Discovery ◽  
Disease Biomarkers ◽  
Urinary Proteome ◽  
Pathological Conditions ◽  
Human Orthologs ◽  
Human Protein Atlas ◽  
Aging Conditions ◽  
Old Rats ◽  
Spectral Counts ◽  
Standard Protein

Urine is a very good source for biomarker discovery because it accumulates the changes of body. The urinary proteome is influenced by various factors, which is a major challenge in urinary biomarker discovery. To circumvent these problems, simpler systems, such as animal models, should be used to establish associations between physiological or pathological conditions and changes in the urinary proteome. In this study, the urinary proteome of young (2-month-old) and old rats (20-month-old; 9 in each group) were analyzed using LC-MS/MS and quantified using the Progenesis LC-MS software. A total of 371 proteins were identified, 194 of which were shared between young and old rats. Based on the criteria of a fold change ≥ 2, P < 0.05 and being identified in each rat in the high abundance group, 33 proteins were changed (15 up-regulated and 18 down-regulated in old rats). By adding a more stringent standard (protein spectral counts from every rat in the higher group greater than those in the lower group), 8 proteins were changed consistently in all rats of between the groups, 2 of which are also altered in the urinary proteome of aging humans. There are no shared proteins between our results and the previous aging plasma proteome. Twenty of the 33 (60 %) changed proteins have been reported to be disease biomarkers, which implies that aging may share similar urinary changes with some diseases. The 33 proteins corresponded to 28 human orthologs, which are strongly expressed in the kidney, intestine, cerebellum and lung, according to the human protein ATLAS. Therefore, the urinary proteome may reflect aging conditions in these organs.

scholarly journals PENGARUH PEMBERIAN SQUARE STEPPING EXERCISE UNTUK MENINGKATKAN KESEIMBANGAN DINAMIS PADA LANSIA

2018 ◽  
Author(s):  
Indah Pramita ◽  
Agus Donny Susanto
Keyword(s):  
Musculoskeletal System ◽  
Dynamic Balance ◽  
The Elderly ◽  
The Body ◽  
P Value ◽  
Visual Ability ◽  
Before And After ◽  
Type Of Exercise ◽  
And Function ◽  
Stepping Exercise

Elderly is an advanced stage process that decreases the ability and function of the body. One of the declines that occur in the elderly is the musculoskeletal system in which it is very influential on balance. Square stepping exercise is one type of exercise that can be used in addition to increasing the strength of the musculoskeletal system, this exercise is also able to improve the visual ability. By increasing the ability of the musculoskeletal system, coupled with the visual ability is expected to improve balance in the elderly and reduce the risk of falling. The purpose of this research is to know the effect of Square stepping exercise on increasing the dynamic balance of elderly.This experimental study used a pre test and post test design. The sampling was done at Panti Wreda Sejahtera Denpasar. Elderly as many as 27 people who fit the criteria serve as a sample. The dynamic balance is evaluated by using the Time Up and Go test (TUG test) before and after the exercise. This study was conducted 3x a week for 4 weeks.Effect analysis test using Paired Sample test obtained p value = 0.001 with a balance increase of 27.92%. The conclusion of research that Giving Square Stepping Exercise can improve dynamic balance in elderly.Keywords: Elderly, Square Stepping Exercixe, Balance

Sign in / Sign up

Export Citation Format

Share Document