Preliminary phytochemical screening, antihyperglycemic, analgesic and toxicity studies on methanolic extract of aerial parts of Corchorus olitorius L.

The study was designed to evaluate the phytoconstituents present in the methanolic extract of aerial parts of Abelmoschus moschatus. The qualitative phytochemical screening of different extracts of aerial parts revealed the presence of some bioactive compounds. GC – MS analysis was performed using Shimadzu Gas chromatography-mass spectroscopy (Model Number: QP2010S) instrument. GC-MS detection of phytoconstituents was done by computer evaluation of mass spectra of samples through National Institute Standard and Technology (NIST II) and WILEY 8 library. GC – MS analysis detected the presence of 14 compounds. GC – MS profile of the methanolic extract revealed the presence of megastigmatrienone, phytol, loliolide, farnesyl acetate, methyl linoleates, gamma-sitosterol, cis, cis, cis-7,10,13-Hexadecatrienal, thymine, pyranone, coumarin, 2 – methoxy 4 – vinyl phenol, guanosine, chinasaure and 3- cyclopentyl propionic acid 2 dimethyl aminoethyl ester. The current study suggests that methanolic extracts of aerial parts of Abelmoschus moschatus contain phytoconstituents with antioxidant and cytoprotective activity. The study results will pave a way for the production of therapeutic agents which can be used for the treatment of various diseases.

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EXTRACTION AND CHARACTERIZATION OF IRAQI ARTEMISIA DRACUNCULUS DRIED AERIAL PARTS EXTRACT THOUGH HPLC AND GC-MS ANALYSIS WITH EVALUATION OF ITS ANTITUMOR ACTIVITY AGAINST 7, 12-DIMETHYLBENZE (a) ANTHRACENE INDUCED SKIN CANCER IN MICE

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i5.16875 ◽

2017 ◽

Vol 9 (5) ◽

pp. 34 ◽

Cited By ~ 1

Author(s):

Nabaa Mohammed Ibrahem

Keyword(s):

Skin Cancer ◽

High Performance ◽

Tumor Volume ◽

Methanolic Extract ◽

Tumor Burden ◽

Phytochemical Screening ◽

Bioactive Constituents ◽

Artemisia Dracunculus ◽

Aerial Parts ◽

Qualitative Estimation

Objective: This study focused on the extraction of bioactive constituents in Iraqi cultivated Artemisia dracunculus L dried aerial parts and phytochemical screening with qualitative estimation of three important flavonols (kampferol, quercetin and rutin). The study also included evaluating the antitumor effects (skin cancer) of methanolic extract for this plant.Methods: Extraction of bioactive constituents was carried out according to standard protocols using 85% methanol. The phytochemical screening has been done using chemical tests and GC/MS with qualitative estimation of three flavonols (kampferol, quercetin and rutin) utilized preparative high-performance liquid chromatography (PHPLC). Skin cancer in mice was induced by using 7, 12-dimethylbenze (a) anthracene to evaluate the antitumor effect of methanolic extract of Artemisia dracunculus when administered orally via intragastric feeding tube. Body weight, tumor volume, tumor burden, percent of tumor incidence and histopathological examination were measured in all tested animals.Results: Phytochemical screening revealed the presence of glycosides, volatile oils, alkaloids, tannin, terpenoids, phenolic compounds and flavonoids. The preparative high-performance liquid chromatography (PHPLC) analysis indicated that rutin had the highest concentration followed by quercetin while kampferol had the lowest concentration. The methanolic extract of the Artemisia dracunculus significantly reduced the percent of tumor incidence, tumor volume and tumor burden of skin cancer in mice (p<0.05).Conclusion: Dried aerial parts of Iraqi Artemisia dracunculus contain many bioactive constituents including three flavonoid compounds (quercetin, kampferol and rutin) that may be responsible for chemo preventive effect exerting by methanolic extract of Artemisia dracunculus against 7, 12-dimethylbenzeanthracine-induced skin cancer in mice.

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Phytochemical Screening and Antibacterial Activity of Libyan Globularia alypum

Khalij-Libya Journal of Dental and Medical Research ◽

10.47705/10.47705/kjdmr.215203 ◽

2021 ◽

pp. 11-22

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Methanolic Extract ◽

Diffusion Method ◽

Dilution Method ◽

Phytochemical Screening ◽

Globularia Alypum ◽

Aerial Parts ◽

Maximum Inhibition ◽

Different Strains

Aims. The aim of this study was to assess the phytochemical screening and antibacterial effect of the aerial parts of G. alypum cultivated in Libya. Methods. The methanolic extract of G. alypum (at concentrations 100 mg/ml, 300 mg/ml, 500 mg/ml and 700 mg/ml) were tested against 13 different strains of standard bacteria (ATCC) by four different methods; paper disc diffusion method, well diffusion method, broth dilution method and finally study the effect of extract on growth curve of bacterial cell were studied. Results. The results of phytochemical screening revealed the presence of flavonoids, phenols, reducing sugars, tannins, saponins, coumarins, steroids, terpenoids, carotenoids, antraquinones and glycosides. While the results of antibacterial tests showed remarkable inhibition of the bacterial growth, with maximum inhibition on growth of Methicillin Resistant Staphylococcus aureus (MRSA) and Staphylococcus aureus. Moderate effect on growth of Enterococcus faecalis, Bacillus subtilis, Aeromonas hydrophila and Salmonella poona. While, the lowest inhibition showed in Yersinia enterocolitica and Listeria monocytogenes. However, the extract did not have any effect on Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella typhimurium and Klabsiella pneumonia. Conclusion. The methanolic extract of Globularia alypumaerial parts contain the most important constituents with positive results during phytochemical screening, and have antibacterial activity against gram positive and gram-negative bacteria.

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EVALUTION OF IN VITRO ANTI-ANGIOGENESIS ACTIVITY ON METHANOLIC EXTRACT OF Clematis buchaniana PLANT

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i2.15348 ◽

2017 ◽

Vol 10 (2) ◽

pp. 227

Author(s):

Abhishek Tiwari

Keyword(s):

Anticancer Agent ◽

Methanolic Extract ◽

Folk Medicine ◽

Chorioallantoic Membrane ◽

Phytochemical Screening ◽

Active Constituents ◽

Physiological Processes ◽

Aerial Parts ◽

Pathological Conditions

Objective: Angiogenesis plays an important role in embryonic development and various physiological processes. However, excessive angiogenesis is associated with several pathological conditions including cancer. Clematis is a genus of about 300 species within the buttercup family Ranunculaceae. It is native to the India, where has been used as folk medicine for the treatment of various ailments. This work is aimed to evaluate the antiangiogenesis activity in the crude methanolic extract of Clematis buchaniana plant.Methods: The entire aerial parts of C. buchaniana were extracted by soxhelation in methanol. Then, the solvent was evaporated to dryness to yield the dried crude extract of C. buchaniana. Then, the extract was subjected to preliminary phytochemical screening to determine the active constituents for effective pharmacological activity. The in-vitro antiangiogenesis effects were later evaluated using chorioallantoic membrane model carried out by incubation in fresh chicken’s eggs.Results: The crude methanolic extract of C. buchaniana was found to have slight ability to inhibit angiogenesis that was evaluated by visualization.Conclusion: C. buchaniana plant extract inhibits angiogenesis by blocking normal vascularization in chick embryo. The ability of inhibiting angiogenic process in eggs by this extract can provide us an herbal anticancer agent in future for further scrutiny.Keywords: Antiangiogenesis, Chorioallantoic membrane, Incubation, Angiogenesis, Clematis buchaniana, Methanolic extract.

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α-Glucosidase Inhibition and Docking Studies of 5-Deoxyflavonols and Dihydroflavonols Isolated from Abutilon pakistanicum

Current Organic Chemistry ◽

10.2174/1385272823666191001224741 ◽

2019 ◽

Vol 23 (17) ◽

pp. 1857-1866

Author(s):

Munawar Hussain ◽

Zaheer Ahmed ◽

Shamsun N. Khan ◽

Syed A. A. Shah ◽

Rizwana Razi ◽

...

Keyword(s):

Methanolic Extract ◽

Docking Studies ◽

Hydroxyl Group ◽

Coumaric Acid ◽

In Vitro Activity ◽

Aerial Parts ◽

First Time ◽

Acid Esters ◽

Phenol Moiety

Three new 5-deoxyflavonoid and dihydroflavonoids 2, 3 and 4 have been isolated from the methanolic extract of Abutioln pakistanicum aerial parts, for which structures were elucidated explicitly by extensive MS- and NMR-experiments. In addition to these, 3,7,4′-trihydroxy-3′-methoxy flavonol (1) is reported for the first time from Abutioln pakistanicum. Compound 2 and 4 are p-coumaric acid esters while compounds 2–4 exhibited α-glucosidase inhibitory activity. Docking studies indicated that the ability of flavonoids 2, 3 and 4 to form multiple hydrogen bonds with catalytically important residues is decisive hence is responsible for the inhibition activity. The docking results signified the observed in-vitro activity quite well which is in accordance with previously obtained conclusion that phenol moiety and hydroxyl group are critical for the inhibition of α-glucosidase enzyme.

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Studies on antibacterial potential and phytochemical screening of different extract of Achyranthes aspera

International Journal of Bioassays ◽

10.21746/ijbio.2016.12.005 ◽

2016 ◽

Vol 5 (12) ◽

pp. 5138

Author(s):

Shyamji Shukla* ◽

Priyanka Soni ◽

Harish K. Kewat

Keyword(s):

Methanolic Extract ◽

Salmonella Typhi ◽

Maximum Activity ◽

Phytochemical Screening ◽

Bacterial Strains ◽

Antibacterial Compounds ◽

Achyranthes Aspera ◽

Biological Sources ◽

Almost All ◽

Antibacterial Potential

There is an alarming increase in the problem of resistance towards antibiotics amongst most of the pathogenic bacterial strains in recent years. This has drawn the attention of researchers around the world to search for novel and eco-friendly antibacterial compounds. Several biological sources have been explored in this respect but medicinal plants have taken a centre stage out of all. Plants have been known as a reservoir of number of bioactive compounds specially the antibacterial ones since time immemorial. Therefore, the present investigation was undertaken to analyze the antibacterial potential of the medicinal plant Achyranthes aspera. This study revealed that highest antibacterial activity was observed in the methanolic extract of stem against almost all test Bacteria. It showed maximum activity against E.coli (30 mm), followed by S. aureus (28 mm), Enterococcus sp.(25mm), Salmonella typhi ( 20 mm) and least activity was recorded in same extract against K.pneumoniae (6 mm). Four phytochemicals were screened in various solvent extracts. They are alkaloid, flavonoids, saponins and tannins.

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Stem Lettuce and Its Metabolites: Does the Variety Make Any Difference?

Foods ◽

10.3390/foods10010059 ◽

2020 ◽

Vol 10 (1) ◽

pp. 59

Author(s):

Janusz Malarz ◽

Klaudia Michalska ◽

Anna Stojakowska

Keyword(s):

Chemical Composition ◽

Plant Material ◽

Methanolic Extract ◽

Sesquiterpene Lactones ◽

Scavenging Activity ◽

Nmr Analysis ◽

Caffeic Acid Derivatives ◽

Aerial Parts ◽

Dpph Scavenging Activity ◽

Dpph Scavenging

The objective of the present study was to characterize chemical composition of hitherto unexamined aerial parts of Lactuca sativa var. angustana cv. Grüner Stern. In contrast to leafy and head varieties of the lettuces, asparagus lettuce grown in Europe is much less studied. Fractionation of a methanolic extract from leaves of L. sativa cv. Grüner Stern, supported with HPLC/DAD and 1H NMR analysis, led to the isolation and/or identification of numerous terpenoid and phenolic compounds, including five apocarotenoids—(-)-loliolide, (+)-dehydrovomifoliol, blumenol A, (6S,9S)-vomifoliol, and corchoionoside C; three sesquiterpene lactones; two lignans—((+)-syringaresinol and its 4-O-β-glucoside); five caffeic acid derivatives; and three flavonoids. Some of the compounds, to the best of our knowledge, have never been isolated from L. sativa before. Moreover, monolignols, phenolic acids and a tryptophan-derived alkaloid were found in the analyzed plant material. Stems, leaves and shoot tips of the asparagus lettuce were examined to assess their phenolics and sesquiterpene lactone content as well as DPPH scavenging activity. Another stem lettuce—L. sativa var. angustana cv. Karola, two cultivars of leafy lettuces and one species of wild lettuce—L. serriola, were also examined as a reference material using HPLC/DAD. The results have been discussed regarding our previous studies and the literature data available.

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Flavon- and Flavonolglycosides from Achillea pannonica Scheele

Zeitschrift für Naturforschung C ◽

10.1515/znc-2001-7-808 ◽

2001 ◽

Vol 56 (7-8) ◽

pp. 521-525 ◽

Cited By ~ 5

Author(s):

Denata Kasaj ◽

Liselotte Krenn ◽

Sonja Prinz ◽

Antje Hüfner ◽

Shi Shan Yuc ◽

...

Keyword(s):

Methanolic Extract ◽

2D Nmr ◽

Flavonoid Glycosides ◽

Spectroscopic Methods ◽

Esi Ms ◽

Aerial Parts ◽

Nmr Techniques ◽

Chemotaxonomic Study ◽

First Time ◽

C Nmr

The detailed investigation of a methanolic extract of aerial parts of Achillea pannonica SCHEELE. within a chemotaxonomic study led to the isolation of 6 flavonoid glycosides. Besides rutin, apigenin-7-O-glucopyranoside, luteolin-7-O-glucopyranoside, apigenin-7-O-rutinoside and acacetin-7-O-rutinoside, an unusual flavondiglucoside was isolated. Its structure was established by UV, 1HNMR and 13C NMR spectroscopic methods including 2D-NMR techniques and ESI-MS as luteolin-7,4′-O-β-diglucoside. This substance is reported for the first time in the genus Achillea. Chemotaxonomic aspects are discussed briefly

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Antidiabetic and Antioxidant Activities of Major Flavonoids of Cynanchum acutum L. (Asclepiadaceae) Growing in Egypt

Zeitschrift für Naturforschung C ◽

10.1515/znc-2008-9-1008 ◽

2008 ◽

Vol 63 (9-10) ◽

pp. 658-662 ◽

Cited By ~ 15

Author(s):

Ghada A. Fawzy ◽

Hossam M. Abdallah ◽

Mohamed S. A. Marzouk ◽

Fathy M. Soliman ◽

Amany A. Sleem

Keyword(s):

Blood Glucose ◽

Spectroscopic Data ◽

Methanolic Extract ◽

Antioxidant Activities ◽

First Report ◽

Insulin Levels ◽

Butanol Fraction ◽

Aerial Parts ◽

First Time

Seven flavonoids were isolated from the butanol fraction of the methanolic extract of the aerial parts of Cynanchum acutum L. (Asclepiadaceae). All of which have been isolated for the first time from the genus Cynanchum. Their structures were established as quercetin 3-O-β-galacturonopyranoside (1), quercetin 7-O-β-glucopyranoside (2), tamarixtin 3-O-β-galacturonopyranoside (3), kaempferol 3-O-β-galacturonopyranoside (4), 8-hydroxyquercetin 3-O-β-galactopyranoside (5), tamarixtin 3-O-α-rhamnopyranoside (6), and tamarixtin 7-O-α-arabinopyranoside (7) on the basis of their chromatographic properties, chemical and spectroscopic data. The major isolated flavonoids 1, 2 and 3 were found to exhibit significant antioxidant and antidiabetic activities (by measuring blood glucose and insulin levels). This is the first report about the antioxidant and antidiabetic activities of compounds 1 - 3.

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PHYTOCHEMICAL SCREENING AND PHARMACOLOGICAL EVALUATION OF AEGLE MARMELOS FRUIT

INDIAN DRUGS ◽

10.53879/id.57.04.12172 ◽

2020 ◽

Vol 57 (04) ◽

pp. 59-64

Author(s):

Pratibha Thaware ◽

Pushpa Karale ◽

Mahesh Karale ◽

Pranati Chavan

Keyword(s):

Methanolic Extract ◽

Radical Scavenging ◽

Reducing Power ◽

Pharmacological Action ◽

Diffusion Method ◽

Phytochemical Screening ◽

Aegle Marmelos ◽

Pharmacological Screening ◽

Hplc Data

The objective of this study was to evaluate the phytochemical and pharmacological screening of fruit extracts of Aegle marmelos using various in vitro methods. The antioxidant activity of the extracts was evaluated by using DPPH free radical scavenging and reducing power method, while well diffusion method was used for antimicrobial activity. Phytochemical screening indicated the presence of flavonoids, and phenolics were abundantly present in methanolic and aqueous extracts of A. marmelos. Methanolic extract showed significant antioxidant potential, which was however inferior to that of ascorbic acid. The methanolic extract showed maximum zone of inhibition against the S. aureus (18 mm) and it was higher than that of the standard tetracycline. The HPLC data predict that the presence of scopoletin, marmelosin and umbelliferone, which may contribute to their pharmacological action.

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